Lysis protein S of phage lambda functions in Saccharomyces cerevisiae.

Abstract:

:The lambda S lysis gene was cloned into a Saccharomyces cerevisiae expression vector under GAL1 control. Induction with galactose in S. cerevisiae terminated cell growth and prevented colony formation. Several membrane proteins immunoreactive with anti-S antibody accumulated in the membranes, indicating that sodium dodecyl sulfate-resistant oligomers of S are formed, similar to those observed in the membranes of Escherichia coli cells killed by expression of the S gene. These observations suggest that the S gene product functions as a cytotoxic protein in the yeast cytoplasmic membrane as it does in the bacterial membrane.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Garrett J,Bruno C,Young R

doi

10.1128/jb.172.12.7275-7277.1990

subject

Has Abstract

pub_date

1990-12-01 00:00:00

pages

7275-7

issue

12

eissn

0021-9193

issn

1098-5530

journal_volume

172

pub_type

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