Amino acid residues in LuxR critical for its mechanism of transcriptional activation during quorum sensing in Vibrio fischeri.

abstract：:The distribution of glycerophospholipid-cholesterol acyltransferase in selected bacterial species was examined. Enzyme activity was demonstrated in cell-free growth media from all members of the family Vibrionaceae which were tested except Plesiomonas shigelloides. In each case, enzyme was produced in exponential to e...

journal_title：Journal of bacteriology

authors： MacIntyre S,Trust TJ,Buckley JT

更新日期：1979-07-01 00:00:00

abstract：:The Escherichia coli adhesin involved in diffuse adherence (AIDA-I) is a multifunctional autotransporter protein that mediates bacterial aggregation and biofilm formation, as well as adhesion and invasion of cultured epithelial cells. To elucidate the structure-function relationships of AIDA-I, we performed transposon...

journal_title：Journal of bacteriology

authors： Charbonneau ME,Mourez M

更新日期：2007-12-01 00:00:00

abstract：:We describe here the identification and characterization of two Listeria monocytogenes (Tn917-LTV3) relA and hpt transposon insertion mutants that were impaired in growth after attachment to a model surface. Both mutants were unable to accumulate (p)ppGpp in response to amino acid starvation, whereas the wild-type str...

journal_title：Journal of bacteriology

authors： Taylor CM,Beresford M,Epton HA,Sigee DC,Shama G,Andrew PW,Roberts IS

更新日期：2002-02-01 00:00:00

abstract：:The homotrimeric enzyme Mt-Cam from Methanosarcina thermophila is the archetype of the gamma class of carbonic anhydrases. A search of databases queried with Mt-Cam revealed that a majority of the homologs comprise a putative subclass (CamH) in which there is major conservation of all of the residues essential for the...

journal_title：Journal of bacteriology

authors： Zimmerman SA,Tomb JF,Ferry JG

更新日期：2010-03-01 00:00:00

abstract：:Cefsulodin lyses actively growing Escherichia coli by binding specifically to penicillin-binding proteins (PBPs) 1a and 1b. Recent findings (F. García del Portillo, M. A. de Pedro, D. Joseleau-Petit, and R. D'Ari, J. Bacteriol. 171:4217-4221, 1989) have linked cefsulodin-induced lysis to septation during the first div...

journal_title：Journal of bacteriology

authors： Jacoby GH,Young KD

更新日期：1991-01-01 00:00:00

abstract：:The global regulator CsrA (carbon storage regulator) is an RNA binding protein that coordinates central carbon metabolism, activates flagellum biosynthesis and motility, and represses biofilm formation in Escherichia coli. CsrA activity is antagonized by the untranslated RNA CsrB, to which it binds and forms a globula...

journal_title：Journal of bacteriology

authors： Suzuki K,Wang X,Weilbacher T,Pernestig AK,Melefors O,Georgellis D,Babitzke P,Romeo T

更新日期：2002-09-01 00:00:00

abstract：:Purified superdormant spores of Bacillus cereus, B. megaterium, and B. subtilis isolated after optimal heat activation of dormant spores and subsequent germination with inosine, d-glucose, or l-valine, respectively, germinate very poorly with the original germinants used to remove dormant spores from spore populations...

journal_title：Journal of bacteriology

authors： Ghosh S,Zhang P,Li YQ,Setlow P

更新日期：2009-09-01 00:00:00

abstract：:Antiserum was made against a single 30S protein, 30S-7. The amount of complement fixed by total 30S protein in the presence of this antiserum indicated that protein 30S-7 was the major antigen in the mixture of proteins. Each of the 30S ribosomal proteins was tested for cross-reactivity with anti-30S-7. This was done ...

journal_title：Journal of bacteriology

authors： Strnad BC,Sypherd PS

更新日期：1971-11-01 00:00:00

abstract：:Cyclic adenosine 3',5'-monophosphate isolated from Mycobacterium smegmatis cells was identified by thin-layer chromatography, stepwise conversion to adenosine 5'-monophosphate and adenosine, ultraviolet absorption spectrum, phosphate analysis, and detection by two relatively specific radioisotopic methods. ...

journal_title：Journal of bacteriology

authors： Lee CH

更新日期：1977-12-01 00:00:00

abstract：:Analysis of a Yersinia pestis Delta caf1A mutant demonstrated that the Caf1A usher is required for the assembly and secretion of the fraction 1 capsule. The capsule assembled into thin fibrils and denser aggregates on the bacterial surface. Pilus-like fibers were also detected on the surface of Y. pestis. The capsule ...

journal_title：Journal of bacteriology

authors： Runco LM,Myrczek S,Bliska JB,Thanassi DG

更新日期：2008-05-01 00:00:00

abstract：:The regulatory system that controls the expression of the Ti plasmid-borne octopine degradation (uad) and transfer (tra) genes in Agrobacterium tumefaciens was studied. A deletion mutant derived from the cointegrate plasmid R702::Ti-B6S3 was isolated, which was compatible with a wild-type Ti plasmid and which had reta...

journal_title：Journal of bacteriology

authors： Klapwijk PM,Schilperoort RA

更新日期：1979-08-01 00:00:00

abstract：:Two types of mutants unable to excrete hemolysin were obtained when E. coli 5K carrying the multicopy hemolytic recombinant plasmid pANN202-312 was mutagenized with Mu d1. One type is altered in the plasmid hly-specific gene, hlyBb, but the other is caused by an insertion of Mu d1 into a chromosomal locus. ...

journal_title：Journal of bacteriology

authors： Juarez A,Goebel W

更新日期：1984-09-01 00:00:00

abstract：:Genes from Bacillus subtilis predicted to encode a phosphate-specific transport (Pst) system were shown by mutation to affect high-affinity Pi uptake but not arsenate resistance or phosphate (Pho) regulation. The transcription start of the promoter upstream of the pstS gene was defined by primer extension. The promote...

journal_title：Journal of bacteriology

authors： Qi Y,Kobayashi Y,Hulett FM

更新日期：1997-04-01 00:00:00

abstract：:Among 30 conjugative plasmids of enteric bacteria from 23 incompatibility (Inc) groups, we found 19 (from 12 Inc groups) which can complement defects caused by a defective single-stranded DNA-binding protein of Escherichia coli K-12. The genes which are responsible for the complementation from three of these plasmids ...

journal_title：Journal of bacteriology

authors： Golub EI,Low KB

更新日期：1985-04-01 00:00:00

abstract：:Insertional mutagenesis was conducted on Bacillus subtilis cells to screen for mutants resistant to catabolite repression. Three classes of mutants that were resistant to glucose-promoted but not mannitol-promoted catabolite repression were identified. Cloning and sequencing of the mutated genes revealed that the muta...

journal_title：Journal of bacteriology

authors： Paulsen IT,Chauvaux S,Choi P,Saier MH Jr

更新日期：1998-02-01 00:00:00

abstract：:Acid titrations of intact and butanol-treated cells of Streptococcus faecalis revealed that nearly all of the intracellular K(+) ions could diffuse into the suspending medium in association with small anions, including ribonucleic acid breakdown products, when the cell membrane was damaged. In contrast, nearly all of ...

journal_title：Journal of bacteriology

authors： Marquis RE,Porterfield N,Matsumura P

更新日期：1973-05-01 00:00:00

abstract：:The nucleotide sequence of the Escherichia coli colicin I receptor gene (cir) has been determined. The predicted mature protein consists of 599 amino acids and has a molecular weight of 67,169. Several previously noted characteristics of other E. coli outer membrane protein sequences were also identified in the sequen...

journal_title：Journal of bacteriology

authors： Nau CD,Konisky J

更新日期：1989-02-01 00:00:00

abstract：:The activity of the ATP:GTP 3'-pyrophosphotransferase (guanosine pentaphosphate synthetase I [GPSI]) from Streptomyces antibioticus is stimulated maximally by methanol at 20% (vol/vol) in assay mixtures. Although the enzyme is not activated by ribosomes, its activity is stimulated by tRNA (uncharged or charged) and by...

journal_title：Journal of bacteriology

authors： Jones GH

更新日期：1994-03-01 00:00:00

abstract：:Dienes, Louis (Massachusetts General Hospital, Boston, Mass.), Bojana M. Bandur, and Sarabelle Madoff. Development of L-type growth in Neisseria gonorrhoeae cultures. J. Bacteriol. 87:1471-1476. 1964.-Strains of Neisseria gonorrhoeae (22) were studied immediately after isolation from patients. After 24 to 48 hr of inc...

journal_title：Journal of bacteriology

authors： DIENES L,BANDUR BM,MADOFF S

更新日期：1964-06-01 00:00:00

abstract：:A major phenotypic trait of the Mycobacterium avium complex is the ability to produce rough and smooth colony variants. The chemical basis of this morphological variation is the loss of an antigenic surface structure, termed glycopeptidolipid (GPL), by rough variants. Using M. avium serovar 2 strain 2151 as a model sy...

journal_title：Journal of bacteriology

authors： Eckstein TM,Inamine JM,Lambert ML,Belisle JT

更新日期：2000-11-01 00:00:00

abstract：:Nucleoside diphosphate kinase (Ndk) is a ubiquitous enzyme which functions in balancing the nucleotide pool of the cell. We have recently reported that in addition to being intracellular in both mucoid and nonmucoid Pseudomonas aeruginosa, Ndk is also secreted into the extracellular environment by mucoid P. aeruginosa...

journal_title：Journal of bacteriology

authors： Kamath S,Chen ML,Chakrabarty AM

更新日期：2000-07-01 00:00:00

abstract：:An ultraviolet (UV)-sensitive mutator gene, mutU, was identified in Escherichia coli K-12. The mutation mutU4 is very close to uvrD, between metE and ilv, on the E. coli chromosome. It was recessive as a mutator and as a UV-sensitive mutation. The frequency of reversion of trpA46 on an F episome was increased by mutU4...

journal_title：Journal of bacteriology

authors： Siegel EC

更新日期：1973-01-01 00:00:00

abstract：:ComX and CSF are Bacillus subtilis extracellular signaling peptides. Many different strains of B. subtilis do not communicate due to strain-specific variation of ComX. We demonstrate that CSF is a species-specific signaling molecule that partially compensates for the lack of ComX-mediated communication between differe...

journal_title：Journal of bacteriology

authors： Pottathil M,Jung A,Lazazzera BA

更新日期：2008-06-01 00:00:00

abstract：:Enzymes involved in (methyl)phenol degradation of Pseudomonas putida H are encoded by the catabolic operon (phlA-L) on plasmid pPGH1. Transcription of this operon by the sigma54 (RpoN)-containing RNA polymerase is positively controlled by the gene product of the divergently transcribed phlR in response to the availabi...

journal_title：Journal of bacteriology

authors： Müller C,Petruschka L,Cuypers H,Burchhardt G,Herrmann H

更新日期：1996-04-01 00:00:00

abstract：:When cells of two auxotrophic mutants of Sulfolobus acidocaldarius are mixed and incubated on solid medium, they form stable genetic recombinants which can be selected, enumerated, and characterized. Any of a variety of auxotrophic markers can recombine with each other, and the phenomenon has been observed at temperat...

journal_title：Journal of bacteriology

authors： Grogan DW

更新日期：1996-06-01 00:00:00

abstract：:A mutation in Escherichia coli leads to the loss of ribosomal protein L24, severely impaired growth, and a temperature-sensitive phenotype. The mutation was shown to be in rplX, the gene for protein L24, and was due to the alteration of an AAA codon to a TAA stop codon at position 61 in rplX that resulted in a 20-amin...

journal_title：Journal of bacteriology

authors： Nishi K,Dabbs ER,Schnier J

更新日期：1985-09-01 00:00:00

abstract：:Site-directed mutagenesis has been used to identify a number of charged residues essential for the transport activity of the PheP protein. These residues are highly conserved in the cluster of amino acid transporters. However, some other conserved residues and a number of aromatic residues have been shown not to be es...

journal_title：Journal of bacteriology

authors： Pi J,Wookey PJ,Pittard AJ

更新日期：1993-11-01 00:00:00

abstract：:Using a transcriptional fusion to the lacZ gene, we have analyzed the anaerobic regulation of the hydrogenase 1 (hya) operon in response to different anaerobic growth conditions and to mutations in regulatory genes. We found that the transcription of the hya operon was induced when the growth condition was changed fro...

journal_title：Journal of bacteriology

authors： Brøndsted L,Atlung T

更新日期：1994-09-01 00:00:00

abstract：:The region of the Rhizobium leguminosarum biovar viciae Sym plasmid pRL1JI, responsible for the production and secretion of a previously described 50-kilodalton protein (R. A. de Maagd, C. A. Wijffelman, E. Pees, and B. J. J. Lugtenberg, J. Bacteriol. 170:4424-4427, 1988), was cloned and its nucleotide sequence was de...

journal_title：Journal of bacteriology

authors： de Maagd RA,Wijfjes AH,Spaink HP,Ruiz-Sainz JE,Wijffelman CA,Okker RJ,Lugtenberg BJ

更新日期：1989-12-01 00:00:00

abstract：:Anaerobic microbial toluene catabolism is initiated by addition of fumarate to the methyl group of toluene, yielding (R)-benzylsuccinate as first intermediate, which is further metabolized via beta-oxidation to benzoyl-coenzyme A (CoA) and succinyl-CoA. A specific succinyl-CoA:(R)-benzylsuccinate CoA-transferase activ...

journal_title：Journal of bacteriology

authors： Leutwein C,Heider J

更新日期：2001-07-01 00:00:00