Chromosomal mutation that affects excretion of hemolysin in Escherichia coli.

abstract：:Cells of the osmotolerant yeast Saccharomyces rouxii were transformed to protoplasts in good yield (85%) by digesting cell walls with snail-gut enzyme in the presence of 10 mM dithioerythritol, 0.1 M sodium phosphate buffer (pH 6.8), and 2.0 M KCl. The requirement for 2.0 M KCl compares with that for S. bisporus var. ...

journal_title：Journal of bacteriology

authors： Arnold WN,Garrison RG

更新日期：1979-03-01 00:00:00

abstract：:Endonuclease V is specific for single-stranded DNA or for duplex DNA that contains uracil or that is damaged by a variety of agents (B. Demple and S. Linn, J. Biol. Chem. 257:2848-2855, 1982). Thus, it may be a versatile DNA repair enzyme. The protein was purified to apparent homogeneity, and from its N-terminal seque...

journal_title：Journal of bacteriology

authors： Guo G,Ding Y,Weiss B

更新日期：1997-01-01 00:00:00

abstract：:Dienes, Louis (Massachusetts General Hospital, Boston, Mass.), Bojana M. Bandur, and Sarabelle Madoff. Development of L-type growth in Neisseria gonorrhoeae cultures. J. Bacteriol. 87:1471-1476. 1964.-Strains of Neisseria gonorrhoeae (22) were studied immediately after isolation from patients. After 24 to 48 hr of inc...

journal_title：Journal of bacteriology

authors： DIENES L,BANDUR BM,MADOFF S

更新日期：1964-06-01 00:00:00

abstract：:We have overexpressed and purified the Helicobacter pylori Fur protein and analyzed its interaction with the intergenic regions of divergent genes involved in iron uptake (frpB and ceuE) and oxygen radical detoxification (katA and tsaA). DNase I footprint analysis showed that Fur binds specifically to a high-affinity ...

journal_title：Journal of bacteriology

authors： Delany I,Pacheco AB,Spohn G,Rappuoli R,Scarlato V

更新日期：2001-08-01 00:00:00

abstract：:PB15 is an Hfr strain of Escherichia coli K-12. It arose from an F' strain carrying a temperature-sensitive F-gal by an event which blocked the detachment of F-gal in the normally reversible integration process. In PB15, the detachment of F-gal by a second mechanism can now be detected: this mechanism results in the e...

journal_title：Journal of bacteriology

authors： Bergquist PL,Adelberg EA

更新日期：1972-07-01 00:00:00

abstract：:The Czc system of Alcaligenes eutrophus mediates resistance to cobalt, zinc, and cadmium through ion efflux catalyzed by the CzcCB2A cation-proton antiporter. DNA sequencing of the region upstream of the czcNICBADRS determinant located on megaplasmid pMOL30 revealed the 5' end of czcN and a gene for a MgtC-like protei...

journal_title：Journal of bacteriology

authors： Grosse C,Grass G,Anton A,Franke S,Santos AN,Lawley B,Brown NL,Nies DH

更新日期：1999-04-01 00:00:00

abstract：:Bacteriophage 7-7-1, a member of the family Myoviridae, infects the soil bacterium Agrobacterium sp. strain H13-3. Infection requires attachment to actively rotating bacterial flagellar filaments, with flagellar number, length, and rotation speed being important determinants for infection efficiency. To identify the s...

journal_title：Journal of bacteriology

authors： Gonzalez F,Helm RF,Broadway KM,Scharf BE

更新日期：2018-09-10 00:00:00

abstract：:In the fungus Podospora anserina, the interaction between the nonallelic incompatible R and V genes has two consequences: a lytic reaction due to the synthesis of specific proteolytic enzymes, and a quenching in protein and ribonucleic acid synthesis. The incompatibility reaction when vegetative or sexual R and V cell...

journal_title：Journal of bacteriology

authors： Labarére J,Bègueret J,Bernet J

更新日期：1974-11-01 00:00:00

abstract：:The hook-basal body (HBB) is a key intermediate structure in the flagellar assembly pathway in Salmonella typhimurium. The FlgM protein inhibits the flagellum-specific transcription factor sigma28 in the absence of the intact HBB structure and is secreted out of the cell following HBB completion. The flk gene encodes ...

journal_title：Journal of bacteriology

authors： Karlinsey JE,Tsui HC,Winkler ME,Hughes KT

更新日期：1998-10-01 00:00:00

abstract：:The penultimate step in the biosynthesis of riboflavin (vitamin B2) involves the condensation of 3,4-dihydroxy-2-butanone 4-phosphate with 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione, which is catalyzed by 6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase). Pathogenic Brucella species adapted to an intr...

journal_title：Journal of bacteriology

authors： Zylberman V,Klinke S,Haase I,Bacher A,Fischer M,Goldbaum FA

更新日期：2006-09-01 00:00:00

abstract：:The proteome of Haemophilus influenzae strain Rd KW20 was analyzed by liquid chromatography (LC) coupled with ion trap tandem mass spectrometry (MS/MS). This approach does not require a gel electrophoresis step and provides a rapidly developed snapshot of the proteome. In order to gain insight into the central metabol...

journal_title：Journal of bacteriology

authors： Kolker E,Purvine S,Galperin MY,Stolyar S,Goodlett DR,Nesvizhskii AI,Keller A,Xie T,Eng JK,Yi E,Hood L,Picone AF,Cherny T,Tjaden BC,Siegel AF,Reilly TJ,Makarova KS,Palsson BO,Smith AL

更新日期：2003-08-01 00:00:00

abstract：:Analysis of the Escherichia coli K10 capsule gene cluster identified two regions, regions 1 and 3, conserved between different group III capsule gene clusters. Region 1 encodes homologues of KpsD, KpsM, KpsT, and KpsE proteins, and region 3 encodes homologues of the KpsC and KpsS proteins. An rfaH mutation abolished K...

journal_title：Journal of bacteriology

authors： Clarke BR,Pearce R,Roberts IS

更新日期：1999-04-01 00:00:00

abstract：:Members of the family Halobacteriaceae in the domain Archaea are obligate extreme halophiles. They occupy a variety of hypersaline environments, and their cellular biochemistry functions in a nearly saturated salty milieu. Despite extensive study, a detailed analysis of their growth kinetics is missing. To remedy this...

journal_title：Journal of bacteriology

authors： Robinson JL,Pyzyna B,Atrasz RG,Henderson CA,Morrill KL,Burd AM,Desoucy E,Fogleman RE 3rd,Naylor JB,Steele SM,Elliott DR,Leyva KJ,Shand RF

更新日期：2005-02-01 00:00:00

abstract：:Twenty-four independent phosphate starvation-inducible (psi) transcriptional fusions made with Mu d1(lacZbla) were analyzed by sequencing the psi::lacZ(Mu d1) chromosomal junctions by using DNAs amplified with the polymerase chain reaction or mini-Mu cloning. Our DNA sequence analysis showed that the MuR DNA in Mu d1 ...

journal_title：Journal of bacteriology

authors： Metcalf WW,Steed PM,Wanner BL

更新日期：1990-06-01 00:00:00

abstract：:Transfer of conjugative plasmids requires relaxases, proteins that cleave one plasmid strand sequence specifically. The F plasmid relaxase TraI (1,756 amino acids) is also a highly processive DNA helicase. The TraI relaxase activity is located within the N-terminal approximately 300 amino acids, while helicase motifs ...

journal_title：Journal of bacteriology

authors： Dostál L,Schildbach JF

更新日期：2010-07-01 00:00:00

abstract：:Pep 5 and nisin are cationic peptide antibiotics which in addition to their membrane-disruptive action induce autolysis in staphylococci. To investigate the mechanism of lysis induction, the influence of the peptides on the activity of the N-acetylmuramoyl-L-alanine amidase of Staphylococcus simulans 22 was studied. I...

journal_title：Journal of bacteriology

authors： Bierbaum G,Sahl HG

更新日期：1987-12-01 00:00:00

abstract：:The intracellular levels of cyclic adenosine 3',5'-monophosphate (cyclic AMP) were measured at various intervals during growth and morphogenesis in Arthrobacter crystallopoietes. Cyclic AMP levels remained relatively constant throughout growth in spherical cells grown in glucose-based media. Immediately after inoculat...

journal_title：Journal of bacteriology

authors： Hamilton RW,Achberger EC,Kolenbrander PE

更新日期：1977-02-01 00:00:00

abstract：:Although 95% of the enterotoxin B produced by Staphylococcus aureus appears during the latter part of the exponential phase of growth, growth per se is not necessary for toxin synthesis. A procedure is described whereby a concentrated suspension (at least 6 x 10(10) cells per ml) of a 16-hr culture of S. aureus was fo...

journal_title：Journal of bacteriology

authors： Markus Z,Silverman GJ

更新日期：1969-02-01 00:00:00

abstract：:Superoxide dismutase (SOD) and catalase (CAT) levels were determined for several aerobically grown halophilic vibrios and compared with those found in aerobically grown Escherichia coli K-12. The SOD levels ranged from 25 to 103.6 U/mg of protein for the vibrios compared with 44.6 U/mg of protein for E. coli. The CAT ...

journal_title：Journal of bacteriology

authors： Daily OP,Debell RM,Joseph SW

更新日期：1978-05-01 00:00:00

abstract：:During development of the symbiotic soil bacterium Sinorhizobium meliloti into nitrogen-fixing bacteroids, DNA replication and cell division cease and the cells undergo profound metabolic and morphological changes. Regulatory genes controlling the early stages of this process have not been identified. As a first step ...

journal_title：Journal of bacteriology

authors： Barnett MJ,Hung DY,Reisenauer A,Shapiro L,Long SR

更新日期：2001-05-01 00:00:00

abstract：:Chronic lung infections in cystic fibrosis (CF) could be treated more effectively if the effects of antimicrobials on pathogens in situ were known. Here, we compared changes in the microbial community composition and pathogen growth rates in longitudinal studies of seven pediatric CF patients undergoing intravenous an...

journal_title：Journal of bacteriology

authors： Neubauer C,Kasi AS,Grahl N,Sessions AL,Kopf SH,Kato R,Hogan DA,Newman DK

更新日期：2018-11-26 00:00:00

abstract：:We constructed a conditional mutant of pbpA in which transcription of the gene was placed under the control of an IPTG (isopropyl-beta-D-thiogalactopyranoside)-inducible promoter in order to explore the role of PBP1 in growth, cell wall structure, and cell division. A methicillin-resistant strain and an isogenic methi...

journal_title：Journal of bacteriology

authors： Pereira SF,Henriques AO,Pinho MG,de Lencastre H,Tomasz A

更新日期：2007-05-01 00:00:00

abstract：:The effects of the free bile acids (FBAs) cholic acid (CA), deoxycholic acid (DCA), and chenodeoxycholic acid on the bioenergetics and growth of lactobacilli and bifidobacteria were investigated. It was found that these FBAs reduced the internal pH levels of these bacteria with rapid and stepwise kinetics and, at cert...

journal_title：Journal of bacteriology

authors： Kurdi P,Kawanishi K,Mizutani K,Yokota A

更新日期：2006-03-01 00:00:00

abstract：:Bacillus brevis 47 contains two cell wall proteins termed the outer wall protein (OWP) and middle wall protein (MWP), each of which forms hexagonal arrays in the cell wall. A 6-kilobase BglII-BclI fragment of B. brevis 47 DNA cloned into Bacillus subtilis with a derivative of pHW1 as a vector directed the synthesis of...

journal_title：Journal of bacteriology

authors： Tsuboi A,Uchihi R,Tabata R,Takahashi Y,Hashiba H,Sasaki T,Yamagata H,Tsukagoshi N,Udaka S

更新日期：1986-10-01 00:00:00

abstract：:A study was made to determine whether factors other than the availability of phosphorus were involved in the regulation of synthesis of teichoic and teichuronic acids in Bacillus subtilis subsp. niger WM. First, the nature of the carbon source was varied while the dilution rate was maintained at about 0.3 h-1. Irrespe...

journal_title：Journal of bacteriology

authors： Kruyssen FJ,de Boer WR,Wouters JT

更新日期：1980-10-01 00:00:00

abstract：:The glpD (MSMEG_6761) gene encoding glycerol-3-phosphate dehydrogenase was shown to be crucial for M. smegmatis to utilize glycerol as the sole carbon source. The glpD gene likely forms the glpFKD operon together with glpF and glpK, encoding a glycerol facilitator and glycerol kinase, respectively. The gylR (MSMEG_675...

journal_title：Journal of bacteriology

authors： Bong HJ,Ko EM,Song SY,Ko IJ,Oh JI

更新日期：2019-11-20 00:00:00

abstract：:Accumulation of certain nonmetabolizable sugar-phosphates (including α-methyl glucoside-6-phosphate) in Escherichia coli is growth inhibitory and elicits the glucose-phosphate stress response. The transcription factor SgrR activates transcription of the small RNA SgrS under stress conditions. SgrS represses translatio...

journal_title：Journal of bacteriology

authors： Sun Y,Vanderpool CK

更新日期：2011-01-01 00:00:00

abstract：:Radiorespirometric and enzymatic analyses reveal that glucose-grown cells of Rhizobium japonicum isolates I-110 and L1-110, both derivatives of R. japonicum strain 3I1b110, possess an active tricarboxylic acid cycle and metabolize glucose by simultaneous operation of the Embden-Meyerhof-Parnas and Entner-Doudoroff pat...

journal_title：Journal of bacteriology

authors： Mulongoy K,Elkan GH

更新日期：1977-07-01 00:00:00

abstract：:Deoxyribonucleic acid (DNA), pulse labeled after ultraviolet irradiation of excision-defective mutants of Haemophilus influenzae, is of lower single strand molecular weight than that of unirradiated cells but approaches the size of DNA from unirradiated cells upon further incubation in growth medium. This gap-filling ...

journal_title：Journal of bacteriology

authors： Small GD

更新日期：1975-10-01 00:00:00

abstract：:The dibenzofuran-degrading bacterial strain DPO360 represents a new species of the genus Terrabacter together with the previously described dibenzofuran-mineralizing bacterial strain DPO1361 (K.-H. Engesser, V. Strubel, K. Christoglou, P. Fischer, and H. G. Rast, FEMS Microbiol. Lett. 65:205-210, 1989; V. Strubel, Ph....

journal_title：Journal of bacteriology

authors： Schmid A,Rothe B,Altenbuchner J,Ludwig W,Engesser KH

更新日期：1997-01-01 00:00:00