Transcriptional organization of the czc heavy-metal homeostasis determinant from Alcaligenes eutrophus.

Abstract:

:The Czc system of Alcaligenes eutrophus mediates resistance to cobalt, zinc, and cadmium through ion efflux catalyzed by the CzcCB2A cation-proton antiporter. DNA sequencing of the region upstream of the czcNICBADRS determinant located on megaplasmid pMOL30 revealed the 5' end of czcN and a gene for a MgtC-like protein which is transcribed in the orientation opposite that of czc. Additional open reading frames upstream of czc had no homologs in the current databases. Using oligonucleotide-probed Northern blotting experiments, a 500-nucleotide czcN message and a 400-nucleotide czcI message were found, and the presence of 6, 200-nucleotide czcCBA message (D. Van der Lelie et al., Mol. Microbiol. 23:493-503, 1997) was confirmed. Induction of czcN, czcI, czcCBA, and czcDRS followed a similar pattern: transcription was induced best by 300 microM zinc, less by 300 microM cobalt, and only slightly by 300 microM cadmium. Reverse transcription-PCR gave evidence for additional continuous transcription from czcN to czcC and from czcD to czcS, but not between czcA and czcD nor between czcS and a 131-amino-acid open reading frame following czcS. The CzcR putative response regulator was purified and shown to bind in the 5' region of czcN. A reporter strain carrying a czcNIC-lacZ-czcBADRS determinant on plasmid pMOL30 was constructed, as were DeltaczcR and DeltaczcS mutants of this strain and of AE128(pMOL30) wild type. Experiments on (i) growth of these strains in liquid culture containing 5 mM Zn2+, (ii) induction of the beta-galactosidase in the reporter strains by zinc, cobalt, and cadmium, and (iii) cDNA analysis of czcCBA mRNA synthesis under inducing and noninducing conditions showed that the CzcRS two-component regulatory system is involved in Czc regulation.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Grosse C,Grass G,Anton A,Franke S,Santos AN,Lawley B,Brown NL,Nies DH

doi

10.1128/JB.181.8.2385-2393.1999

keywords:

subject

Has Abstract

pub_date

1999-04-01 00:00:00

pages

2385-93

issue

8

eissn

0021-9193

issn

1098-5530

journal_volume

181

pub_type

杂志文章
  • Lysozyme-promoted association of protein I molecules in the outer membrane of Escherichia coli.

    abstract::Incubation of whole envelopes prepared from sonically oscillated Escherichia coli K-12 cultures with lysozyme in vitro resulted in the appearance of a protein species with an apparent molecular weight double that of outer membrane protein I. Similar dimers were also detected in purified outer membranes and whole envel...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.132.2.411-418.1977

    authors: Chopra I,Howe GB,Ball PR

    更新日期:1977-11-01 00:00:00

  • Induction of anaerobic gene expression in Rhodobacter capsulatus is not accompanied by a local change in chromosomal supercoiling as measured by a novel assay.

    abstract::In the photosynthetic bacterium Rhodobacter capsulatus, the enzyme DNA gyrase has been implicated in the expression of genes for anaerobic metabolic processes such as nitrogen fixation and photosynthesis. To assess the involvement of supercoiling in anaerobic gene expression, we have developed an assay to detect in vi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.9.4836-4843.1989

    authors: Cook DN,Armstrong GA,Hearst JE

    更新日期:1989-09-01 00:00:00

  • Deoxyribonucleic acid sequence organization of a yeast plasmid.

    abstract::Two-micrometer deoxyribonucleic acid (DNA), a circular plasmid of Saccharomyces cerevisiae, contains two nontandem repeated sequences which are inverted with respect to one another. These repeated sequences together account for 21% of the molecule length. Restriction endonuclease analysis and electron microscopy demon...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.129.1.472-481.1977

    authors: Livingston DM,Klein HL

    更新日期:1977-01-01 00:00:00

  • Properties of Bacillus megaterium temperature-sensitive germination mutants.

    abstract::Bacillus megaterium mutants JV-9 and JV-10 are temperature sensitive for initiation of spore germination. At 46 C, they did not lose heat resistance, dipicolinic acid, or absorbance, indicating that the temperature-sensitive blocks are very early in the sequence of initiation reactions. Strain JV-9 was temperature sen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.121.1.197-203.1975

    authors: Vary JC

    更新日期:1975-01-01 00:00:00

  • Studies of intestinal microflora. VI. Effect of x irradiation on the fecal microflora of the rat.

    abstract::The effect of sublethal, midlethal, and lethal doses of total-body X irradiation on the fecal microbial population of the rat was studied. No changes were observed in animals receiving sublethal doses of X ray. Midlethal and lethal doses produced an increase in the numbers of fecal coliforms, staphylococci, streptococ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.2.378-382.1967

    authors: Klainer AS,Gorbach S,Weinstein L

    更新日期:1967-08-01 00:00:00

  • Bacteriostatic activity of serum against staphylococci.

    abstract::Cybulska, Janina (State Institute of Hygiene, Warsaw, Poland), and J. Jeljaszewicz. Bacteriostatic activity of serum against staphylococci. J. Bacteriol. 91:953-962. 1966.-Antistaphylococcal activity of normal serum against strains exhibiting various patterns of coagulase, clumping-factor, and staphylokinase productio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.3.953-962.1966

    authors: Cybulska J,Jeljaszewicz J

    更新日期:1966-03-01 00:00:00

  • The Pseudomonas stutzeri-Specific Regulatory Noncoding RNA NfiS Targets katB mRNA Encoding a Catalase Essential for Optimal Oxidative Resistance and Nitrogenase Activity.

    abstract::Pseudomonas stutzeri A1501 is a versatile nitrogen-fixing bacterium capable of living in diverse environments and coping with various oxidative stresses. NfiS, a regulatory noncoding RNA (ncRNA) involved in the control of nitrogen fixation in A1501, was previously shown to be required for optimal resistance to H2O2; h...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00334-19

    authors: Zhang H,Zhan Y,Yan Y,Liu Y,Hu G,Wang S,Yang H,Qiu X,Liu Y,Li J,Lu W,Elmerich C,Lin M

    更新日期:2019-09-06 00:00:00

  • Role of protein degradation in the survival of carbon-starved Escherichia coli and Salmonella typhimurium.

    abstract::When an Escherichia coli K-12 culture was starved for glucose, 50% of the cells lost viability in about 6 days. When a K-12 mutant lacking five distinct peptidase activities, CM89, was starved in the same manner, viability was lost much more rapidly; 50% of the cells lost viability in about 2 days, whereas a parent st...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.157.3.758-763.1984

    authors: Reeve CA,Bockman AT,Matin A

    更新日期:1984-03-01 00:00:00

  • Identification and characterization of IS1411, a new insertion sequence which causes transcriptional activation of the phenol degradation genes in Pseudomonas putida.

    abstract::A new insertion sequence (IS element), IS1411, was identified downstream of the phenol degradation genes pheBA that originated from plasmid DNA of Pseudomonas sp. strain EST1001. According to sequence analysis, IS1411 belongs to a new family of IS elements that has recently been named the ISL3 family (J. Mahillon and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.20.5306-5312.1998

    authors: Kallastu A,Hõrak R,Kivisaar M

    更新日期:1998-10-01 00:00:00

  • The dimerization function of MinC resides in a structurally autonomous C-terminal domain.

    abstract::Limited proteolysis of the Escherichia coli cell division inhibitor MinC reveals that its dimerization function resides in a structurally autonomous C-terminal domain. We show that cytoplasmic MinC is poised near the monomer-dimer equilibrium and propose that it only becomes entirely dimeric once recruited to the memb...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.22.6684-6687.2001

    authors: Szeto TH,Rowland SL,King GF

    更新日期:2001-11-01 00:00:00

  • Transport of nonmetabolizable opines by Agrobacterium tumefaciens.

    abstract::We have examined the uptake of [14C]octopine and [14C]nopaline by Agrobacterium tumefaciens strains containing the C58 chromosomal background in medium suitable for the induction of vir genes. All strains tested could transport both of these opines, regardless of the presence or type of Ti plasmid (octopine or nopalin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.2.903-905.1991

    authors: Krishnan M,Burgner JW,Chilton WS,Gelvin SB

    更新日期:1991-01-01 00:00:00

  • Phosphopantetheine adenylyltransferase from Escherichia coli: investigation of the kinetic mechanism and role in regulation of coenzyme A biosynthesis.

    abstract::Phosphopantetheine adenylyltransferase (PPAT) from Escherichia coli is an essential hexameric enzyme that catalyzes the penultimate step in coenzyme A (CoA) biosynthesis and is a target for antibacterial drug discovery. The enzyme utilizes Mg-ATP and phosphopantetheine (PhP) to generate dephospho-CoA (dPCoA) and pyrop...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00732-07

    authors: Miller JR,Ohren J,Sarver RW,Mueller WT,de Dreu P,Case H,Thanabal V

    更新日期:2007-11-01 00:00:00

  • Isolation and partial characterization of protein E, a major protein found in certain Escherichia coli K-12 mutant strains: relationship to other outer membrane proteins.

    abstract::Escherichia coli outer membrane protein E was purified, and its amino acid composition and N-terminal amino acid were determined. The purified protein was shown to be immunologically and electrophoretically identical to proteins Ic (U. Henning, W. Schmidmayr, and I. Hindennach, Mol. Gen. Genet. 154:293-298, 1977) and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.139.2.418-423.1979

    authors: Chai TJ,Foulds J

    更新日期:1979-08-01 00:00:00

  • Purification and properties of phytate-specific phosphatase from Bacillus subtilis.

    abstract::An enzyme which liberates Pi from myo-inositol hexaphosphate (phytic acid) was shown to be present in culture filtrates of Bacillus subtilis. It was purified until it was homogeneous by ultracentrifugation, but it still showed two isozymes on polyacrylamide gel electrophoresis. The enzyme differed from other previousl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.151.3.1102-1108.1982

    authors: Powar VK,Jagannathan V

    更新日期:1982-09-01 00:00:00

  • Methods for the determination of deoxyribonucleic Acid homologies in streptomyces.

    abstract::Variations of the membrane filter technique for deoxyribonucleic acid (DNA) hybridizations were studied with respect to Streptomyces species. At the temperatures required for specific hybridization of DNA with the high melting temperature (T(m)) characteristic of Streptomyces, large amounts (up to 97%) of filter-bound...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.104.3.1086-1094.1970

    authors: Okanishi M,Gregory KF

    更新日期:1970-12-01 00:00:00

  • Genetic and molecular analyses of the gene encoding staphylococcal enterotoxin D.

    abstract::The gene (entD) encoding staphylococcal enterotoxin D (SED) has been located on a 27.6-kilobase penicillinase plasmid designated pIB485. This plasmid was present in all SED-producing strains tested. The entD gene was cloned on a 2.0-kilobase DNA fragment and was expressed in Escherichia coli. Sequence analysis of this...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.9.4799-4806.1989

    authors: Bayles KW,Iandolo JJ

    更新日期:1989-09-01 00:00:00

  • Cell-free mercury(II)-reducing activity in a plasmid-bearing strain of Escherichia coli.

    abstract::The ability to reduce Hg(II) to Hg(0), which is determined by a plasmid-borne gene in Escherichia coli, is conferred by a Hg(II)-inducible activity which is located in the cytoplasm rather than in the periplasmic space of the cell. This Hg(II)-reducing activity can be isolated from the supernatant of a 160,000 x g cen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.119.1.242-249.1974

    authors: Summers AO,Sugarman LI

    更新日期:1974-07-01 00:00:00

  • Evidence of complex transcriptional, translational, and posttranslational regulation of the extracytoplasmic function sigma factor sigmaE in Mycobacterium tuberculosis.

    abstract::The extracytoplasmic factor (ECF) sigma factor sigma(E) is one of the most studied sigma factors of Mycobacterium tuberculosis. It has been shown to be involved in virulence as well as in survival under conditions of high temperature, alkaline pH, and exposure to detergents and oxidative stress. Unlike many ECF sigma ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00622-08

    authors: Donà V,Rodrigue S,Dainese E,Palù G,Gaudreau L,Manganelli R,Provvedi R

    更新日期:2008-09-01 00:00:00

  • Cell growth inhibition upon deletion of four toxin-antitoxin loci from the megaplasmids of Sinorhizobium meliloti.

    abstract::Toxin and antitoxin (TA) gene pairs are addiction systems that are present in many microbial genomes. Sinorhizobium meliloti is an N2-fixing bacterial symbiont of alfalfa and other leguminous plants, and its genome consists of three large replicons, a circular chromosome (3.7 Mb) and the megaplasmids pSymA (1.4 Mb) an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01104-13

    authors: Milunovic B,diCenzo GC,Morton RA,Finan TM

    更新日期:2014-02-01 00:00:00

  • Tyrosine 106 of CheY plays an important role in chemotaxis signal transduction in Escherichia coli.

    abstract::CheY is the response regulator in the signal transduction pathway of bacterial chemotaxis. Position 106 of CheY is occupied by a conserved aromatic residue (tyrosine or phenylalanine) in the response regulator superfamily. A number of substitutions at position 106 have been made and characterized by both behavioral an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.14.4208-4215.1996

    authors: Zhu X,Amsler CD,Volz K,Matsumura P

    更新日期:1996-07-01 00:00:00

  • Two nifA-like genes required for expression of alternative nitrogenases by Azotobacter vinelandii.

    abstract::Two nifA-like genes, designated anfA and vnfA, have been identified in Azotobacter vinelandii. The anfA gene is located upstream from the nitrogenase-3 structural gene cluster (anfHDGK) and is preceded by a sequence that is potentially part of a ntrA-dependent promoter. The product of anfA appears to be required for e...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.6.3258-3267.1989

    authors: Joerger RD,Jacobson MR,Bishop PE

    更新日期:1989-06-01 00:00:00

  • Bacteriocin activity of Streptococcus pneumoniae is controlled by the serine protease HtrA via posttranscriptional regulation.

    abstract::The blp locus of a type 6A strain of Streptococcus pneumoniae encodes a two-peptide bacteriocin, pneumocin MN, which mediates intraspecies competition during mouse nasopharyngeal colonization. This locus is regulated by a quorum-sensing mechanism consisting of a dedicated two-component regulatory system and a peptide ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01213-08

    authors: Dawid S,Sebert ME,Weiser JN

    更新日期:2009-03-01 00:00:00

  • Effective symbiosis between Rhizobium etli and Phaseolus vulgaris requires the alarmone ppGpp.

    abstract::The symbiotic interaction between Rhizobium etli and Phaseolus vulgaris, the common bean plant, ultimately results in the formation of nitrogen-fixing nodules. Many aspects of the intermediate and late stages of this interaction are still poorly understood. The R. etli relA gene was identified through a genome-wide sc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.15.5460-5469.2005

    authors: Moris M,Braeken K,Schoeters E,Verreth C,Beullens S,Vanderleyden J,Michiels J

    更新日期:2005-08-01 00:00:00

  • A complex network regulates expression of eps and other virulence genes of Pseudomonas solanacearum.

    abstract::We have discovered an unusual and complex regulatory network used by the phytopathogen Pseudomonas solanacearum to control transcription of eps, which encodes for production of its primary virulence factor, the exopolysaccharide EPS I. The major modules of this network were shown to be three separate signal transducti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.5.1259-1267.1995

    authors: Huang J,Carney BF,Denny TP,Weissinger AK,Schell MA

    更新日期:1995-03-01 00:00:00

  • Draft genome sequence of the quality control strain Enterococcus faecalis ATCC 29212.

    abstract::Enterococcus faecalis ATCC 29212, a vancomycin-sensitive strain, has been extensively used as a representative control strain for clinical and laboratory experiments. Here we report the draft genome and annotation of this strain, containing 3,027,060 bp, with a G+C content of 37.2% in 126 contigs (≥ 500 bp). ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01423-12

    authors: Kim EB,Kopit LM,Harris LJ,Marco ML

    更新日期:2012-11-01 00:00:00

  • Complete genome sequence of Halalkalicoccus jeotgali B3(T), an extremely halophilic archaeon.

    abstract::Halalkalicoccus jeotgali B3(T), isolated from salt-fermented seafood from South Korea, is an extremely halophilic archaeon belonging to the family Halobacteriaceae. Here, we present the complete genome sequence of the type strain H. jeotgali B3(T) (3,698,650 bp, with a G+C content of 62.5%), which consists of one chro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00663-10

    authors: Roh SW,Nam YD,Nam SH,Choi SH,Park HS,Bae JW

    更新日期:2010-09-01 00:00:00

  • Lag in adaptation to lactose as a probe to the timing of permease incorporation into the cell membrane.

    abstract::If bacteria are incapable of forming and incorporating proteins into the cytoplasmic membranes in all phases of the cell cycle, then not all cells from an asynchronous culture should be capable of growth when switched to a new carbon and energy source whose metabolism requires new membrane function. The transfer of an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.124.1.435-444.1975

    authors: Koch AL

    更新日期:1975-10-01 00:00:00

  • Effects of the Calvin cycle on nicotinamide adenine dinucleotide concentrations and redox balances of Xanthobacter flavus.

    abstract::The levels of reduced and oxidized nicotinamide adenine dinucleotides were determined in Xanthobacter flavus during a transition from heterotrophic to autotrophic growth. Excess reducing equivalents are rapidly dissipated following induction of the Calvin cycle, indicating that the Calvin cycle serves as a sink for ex...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.16.4637-4639.2000

    authors: van Keulen G,Dijkhuizen L,Meijer WG

    更新日期:2000-08-01 00:00:00

  • Analysis of the dynamics of a Bacillus subtilis spore germination protein complex during spore germination and outgrowth.

    abstract::Germination of Bacillus subtilis spores is normally initiated when nutrients from the environment interact with germinant receptors (GRs) in the spores' inner membrane (IM), in which most of the lipids are immobile. GRs and another germination protein, GerD, colocalize in the IM of dormant spores in a small focus term...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02274-14

    authors: Troiano AJ Jr,Zhang J,Cowan AE,Yu J,Setlow P

    更新日期:2015-01-01 00:00:00

  • Transcriptional analysis of the Streptomyces glaucescens tetracenomycin C biosynthesis gene cluster.

    abstract::A 12.6-kb DNA fragment from Streptomyces glaucescens GLA.0 containing the 12 genes for tetracenomycin (TCM) C biosynthesis and resistance enabled Streptomyces lividans to produce TCM C. Transcriptional analysis of the tcmPG intergenic region in this cluster established the presence of two divergent promoters. The tcmI...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.12.3887-3892.1993

    authors: Decker H,Hutchinson CR

    更新日期:1993-06-01 00:00:00