A novel cell surface polysaccharide in Pseudomonas putida WCS358, which shares characteristics with Escherichia coli K antigens, is not involved in root colonization.


:Previously we have shown that flagella and the O-specific polysaccharide of lipopolysaccharide play a role in colonization of the potato root by plant growth-promoting Pseudomonas strains WCS374 and WCS358. In this paper, we describe a novel cell surface-exposed structure in Pseudomonas putida WCS358 examined with a specific monoclonal antibody. This cell surface structure appeared to be a polysaccharide, which was accessible to the monoclonal antibody at the outer cell surface. Further study revealed that it does not contain 2-keto-3-deoxyoctonate, heptose, or lipid A, indicating that it is not a second type of lipopolysaccharide. Instead, the polysaccharide shared some characteristics with K antigen described for Escherichia coli. From a series of 49 different soil bacteria tested, only one other potato plant growth-promoting Pseudomonas strain reacted positively with the monoclonal antibody. Mutant cells lacking the novel antigen were efficiently isolated by an enrichment method involving magnetic antibodies. Mutant strains defective in the novel antigen contained normal lipopolysaccharide. One of these mutants was affected in neither its ability to adhere to sterile potato root pieces nor its ability to colonize potato roots. We conclude that the bacterial cell surface of P. putida WCS358 contains at least two different polysaccharide structures. These are the O-specific polysaccharide of lipopolysaccharide, which is relevant for potato root colonization, and the novel polysaccharide, which is not involved in adhesion to or colonization of the potato root.


J Bacteriol


Journal of bacteriology


de Weger LA,Bloemberg GV,van Wezel T,van Raamsdonk M,Glandorf DC,van Vuurde J,Jann K,Lugtenberg BJ




Has Abstract


1996-04-01 00:00:00












  • Analysis of the binding site of the LysR-type transcriptional activator TcbR on the tcbR and tcbC divergent promoter sequences.

    abstract::The TcbR transcriptional activator protein, which is encoded by the tcbR gene of Pseudomonas sp. strain P51 (J. R. van der Meer, A. C. J. Frijters, J. H. J. Leveau, R. I. L. Eggen, A. J. B. Zehnder, and W. M. de Vos, J. Bacteriol. 173:3700-3708, 1991), was purified from overproducing Escherichia coli cells by using a ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Leveau JH,de Vos WM,van der Meer JR

    更新日期:1994-04-01 00:00:00

  • Roles of catabolite activator protein sites centered at -81.5 and -41.5 in the activation of the Klebsiella aerogenes histidine utilization operon hutUH.

    abstract::The Klebsiella aerogenes hutUH operon is preceded by a promoter region, hut(P), that contains two divergent promoters (hutUp and Pc) which overlap and are alternately expressed. In the absence of the catabolite gene activator protein-cyclic AMP (CAP-cAMP) complex, Pc is predominantly expressed while hutUp is largely r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Osuna R,Janes BK,Bender RA

    更新日期:1994-09-01 00:00:00

  • Dependence of Escherichia coli hyperbaric oxygen toxicity on the lipid acyl chain composition.

    abstract::This study examines certain membrane-related aspects of oxygen poisoning in Escherichia coli K1060 (fabB fadE lacI) and its parent strain, K-12 Ymel. Cells were grown to exponential or stationary phase in a minimal medium and exposed to air plus 300 lb/in2 of O2 as a suspension in minimal salts. After an initial lag, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Harley JB,Santangelo GM,Rasmussen H,Goldfine H

    更新日期:1978-06-01 00:00:00

  • Genetic regulation of glycogen biosynthesis in Escherichia coli: in vitro effects of cyclic AMP and guanosine 5'-diphosphate 3'-diphosphate and analysis of in vivo transcripts.

    abstract::Glycogen accumulation in Escherichia coli is inversely related to the growth rate and occurs most actively when cells enter the stationary phase. The levels of the three biosynthetic enzymes undergo corresponding changes under these conditions, suggesting that genetic control of enzyme biosynthesis may account for at ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Romeo T,Preiss J

    更新日期:1989-05-01 00:00:00

  • Characterization of a periplasmic ATP-binding cassette iron import system of Brachyspira (Serpulina) hyodysenteriae.

    abstract::The nucleotide sequence of the pathogenic spirochete Brachyspira hyodysenteriae bit (for "Brachyspira iron transport") genomic region has been determined. The bit region is likely to encode an iron ATP-binding cassette transport system with some homology to those encountered in gram-negative bacteria. Six open reading...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Dugourd D,Martin C,Rioux CR,Jacques M,Harel J

    更新日期:1999-11-01 00:00:00

  • Loss and repair of conjugal fertility and infectivity of the resistance factor and sex factor in Escherichia coli.

    abstract::Hirota, Yukinori (University of Osaka, Osaka, Japan), Toshio Fujii, and Yukinobu Nishimura. Loss and repair of conjugal fertility and infectivity of the resistance factor and sex factor in Escherichia coli. J. Bacteriol. 91:1298-1304. 1966.-The drug-resistance factor, R, and the sex factor, F, have homologous traits, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hirota Y,Fujii T,Nishimua Y

    更新日期:1966-03-01 00:00:00

  • Extracellular collagenase from Trichophyton schoenleinii.

    abstract::The dermatophyte Trichophyton schoenleinii elaborates an extracellular collagenase which is specific for native collagen and gelatin and is separable from an enzyme which is active against Azocoll. The fungal enzyme differs from the bacterial collagenases in its acid pH and small molecular weight. In addition, the fun...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Rippon JW

    更新日期:1968-01-01 00:00:00

  • Relationship between proton motive force and motility in Spirochaeta aurantia.

    abstract::The effects of various metabolic inhibitors on the motility of Spirochaeta aurantia were investigated. After 15 min in sodium arsenate buffer, 90% of cells remained motile even though adenosine triphosphate levels dropped from 5.6 to 0.1 nmol/mg (dry weight) of cells. After 70 min in sodium arsenate, 5% of cells were ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Goulbourne EA Jr,Greenberg EP

    更新日期:1980-09-01 00:00:00

  • Selection of fimbriate transductants of Salmonella typhimurium dependent on motility.

    abstract::The ability to form type 1 fimbriae (Fim(+)) was readily transduced to 159 out of 161 wild-type motile Fim(-) FIRN strains of Salmonella typhimurium with phage P22 propagated on a Fim(+) donor strain. Fim(+) clones were isolated from about 35% of tests after the fimbriate bacteria in the transduction mixture had been ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Old DC,Duguid JP

    更新日期:1971-09-01 00:00:00

  • The mRNA for an inducible chloramphenicol acetyltransferase gene is cleaved into discrete fragments in Bacillus subtilis.

    abstract::cat-86 is a promoter-deficient plasmid gene that encodes chloramphenicol acetyltransferase (CAT). Insertion of a promoter at a site 144 base pairs 5' to the cat-86 coding sequence activates transcription of the gene and allows cat-86 to specify chloramphenicol-inducible CAT activity in Bacillus subtilis. Induction of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ambulos NP Jr,Duvall EJ,Lovett PS

    更新日期:1987-03-01 00:00:00

  • Fusion of yeast spheroplasts.

    abstract::Spheroplasts of two different auxotrophic strains of Saccharomyces cerevisiae, both of mating type a, were fused with the aid of polyethylene glycol and calcium ions. After reversion to vegetative cells in solid media, the resulting zygotes were shown to be diploid cells of mating type aa. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: van Solingen P,van der Plaat JB

    更新日期:1977-05-01 00:00:00

  • Genome sequence of the chemolithoautotrophic bacterium Oligotropha carboxidovorans OM5T.

    abstract::Oligotropha carboxidovorans OM5(T) (DSM 1227, ATCC 49405) is a chemolithoautotrophic bacterium with the capability to utilize carbon monoxide, carbon dioxide, and hydrogen. It is also capable of heterotrophic growth under appropriate environmental conditions. Here we report the annotated genome sequence of the circula...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Paul D,Bridges S,Burgess SC,Dandass Y,Lawrence ML

    更新日期:2008-08-01 00:00:00

  • Rapid identification of bacterial genes that are lethal when cloned on multicopy plasmids.

    abstract::A procedure to identify genes that are lethal when cloned on multicopy plasmids was developed. It depends on the ability of mini-Mu plasmid elements to be used for both in vivo cloning and generalized transduction of enterobacterial genes. The feasibility of this procedure was demonstrated by using the tetA gene of Tn...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Berg CM,Liu L,Wang B,Wang MD

    更新日期:1988-01-01 00:00:00

  • Cloning and nucleotide sequencing of Rhizobium meliloti aminotransferase genes: an aspartate aminotransferase required for symbiotic nitrogen fixation is atypical.

    abstract::In Rhizobium meliloti, an aspartate aminotransferase (AspAT) encoded within a 7.3-kb HindIII fragment was previously shown to be required for symbiotic nitrogen fixation and aspartate catabolism (V. K. Rastogi and R.J. Watson, J. Bacteriol. 173:2879-2887, 1991). A gene coding for an aromatic aminotransferase located w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Watson RJ,Rastogi VK

    更新日期:1993-04-01 00:00:00

  • Cyclic diguanylic acid and cellulose synthesis in Agrobacterium tumefaciens.

    abstract::The occurrence of the novel regulatory nucleotide bis(3',5')-cyclic diguanylic acid (c-di-GMP) and its relation to cellulose biogenesis in the plant pathogen Agrobacterium tumefaciens was studied. c-di-GMP was detected in acid extracts of 32P-labeled cells grown in various media, and an enzyme responsible for its form...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Amikam D,Benziman M

    更新日期:1989-12-01 00:00:00

  • Characterization of the membrane-bound succinic dehydrogenase of Micrococcus lysodeikticus.

    abstract::The occurrence of succinic dehydrogenase [succinic:(acceptor) oxidoreductase, EC] in membrane fractions of Micrococcus lysodeikticus was investigated. The enzyme could be purified 10-fold, by deoxycholate treatment. Butanol extraction of membranes yielded an active fraction, nonsedimentable at 130,000 x g for...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Pollock JJ,Linder R,Salton MR

    更新日期:1971-07-01 00:00:00

  • Vibrio cholerae enterotoxin genes: nucleotide sequence analysis of DNA encoding ADP-ribosyltransferase.

    abstract::The nucleotide sequence of the DNA encoding the ADP-ribosyltransferase (A1) fragment of cholera enterotoxin was determined. A putative precursor of the A1 peptide contains an 18-amino acid leader peptide, and the mature A1 peptide contains 194 amino acids. The primary structure of the A1 fragment from cholera enteroto...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lockman HA,Galen JE,Kaper JB

    更新日期:1984-09-01 00:00:00

  • Salmonella produces an O-antigen capsule regulated by AgfD and important for environmental persistence.

    abstract::In this study, we show that Salmonella produces an O-antigen capsule coregulated with the fimbria- and cellulose-associated extracellular matrix. Structural analysis of purified Salmonella extracellular polysaccharides yielded predominantly a repeating oligosaccharide unit similar to that of Salmonella enterica serova...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Gibson DL,White AP,Snyder SD,Martin S,Heiss C,Azadi P,Surette M,Kay WW

    更新日期:2006-11-01 00:00:00

  • Selection for citrate synthase deficiency in icd mutants of Escherichia coli.

    abstract::Cultures of isocitrate dehydrogenase-deficient (icd) mutants were overgrown by double mutants (icd glt) lacking citrate synthase activity also. The icd mutants grew more slowly than wild-type cells or the double mutants because they accumulated an inhibitory metabolite (possibly citrate). Intracellular citrate levels ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lakshmi TM,Helling RB

    更新日期:1976-07-01 00:00:00

  • Oxygen control of the Bradyrhizobium japonicum hemA gene.

    abstract::The hemA gene of Bradyrhizobium japonicum, which encodes the first enzyme in the heme biosynthetic pathway, is regulated by oxygen. Up to ninefold induction of beta-galactosidase activity is seen when cultures of B. japonicum containing either a plasmid-encoded or a chromosomally integrated hemA-lacZ fusion are shifte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Page KM,Guerinot ML

    更新日期:1995-07-01 00:00:00

  • Proteolytic Cleavage of the Immunodominant Outer Membrane Protein rOmpA in Rickettsia rickettsii.

    abstract::Rickettsia rickettsii, the causative agent of Rocky Mountain spotted fever, contains two immunodominant proteins, rOmpA and rOmpB, in the outer membrane. Both rOmpA and rOmpB are conserved throughout spotted fever group rickettsiae as members of a family of autotransporter proteins. Previously, it was demonstrated tha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Noriea NF,Clark TR,Mead D,Hackstadt T

    更新日期:2017-02-28 00:00:00

  • Homology with a repeated Yersinia pestis DNA sequence IS100 correlates with pesticin sensitivity in Yersinia pseudotuberculosis.

    abstract::We have identified IS100 sequences in a specific subset of Yersinia pseudotuberculosis isolates that were also sensitive to the Y. pestis-produced bacteriocin, pesticin. In contrast, Y. pseudotuberculosis strains which did not contain IS100 sequences were not sensitive to pesticin. We propose that IS100 serves as a mo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: McDonough KA,Hare JM

    更新日期:1997-03-01 00:00:00

  • Genetics and biochemistry of pyrimidine biosynthesis in Bacillus subtilis: linkage between mutations resulting in a requirement for uracil.

    abstract::A number of independently derived uracil-requiring mutant strains of the parent strain 168 were mapped by inter-mutant transformation crosses. Only a few of these mutant strains were found to be transformable. Studies were performed in which these transformable uracil-requiring mutant strains were used as the recipien...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kelleher RJ Jr,Gooder H

    更新日期:1973-11-01 00:00:00

  • Flavobacterium johnsoniae gldN and gldO are partially redundant genes required for gliding motility and surface localization of SprB.

    abstract::Cells of the gliding bacterium Flavobacterium johnsoniae move rapidly over surfaces. Mutations in gldN cause a partial defect in gliding. A novel bacteriophage selection strategy was used to aid construction of a strain with a deletion spanning gldN and the closely related gene gldO in an otherwise wild-type F. johnso...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Rhodes RG,Samarasam MN,Shrivastava A,van Baaren JM,Pochiraju S,Bollampalli S,McBride MJ

    更新日期:2010-03-01 00:00:00

  • L-cysteine biosynthesis in Bacillus subtilis: identification, sequencing, and functional characterization of the gene coding for phosphoadenylylsulfate sulfotransferase.

    abstract::Random Tn917 mutagenesis of Bacillus subtilis followed by selection of lipoic acid auxotrophs led to the isolation of the cysH gene. The gene was sequenced and found to encode a phosphoadenylylsulfate sulfotransferase with a molecular mass of 27 kDa. Expression of lacZ fused to the cysH promoter was repressed by cyste...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Mansilla MC,de Mendoza D

    更新日期:1997-02-01 00:00:00

  • In vivo DNA binding of bacteriophage GA-1 protein p6.

    abstract::Bacteriophage GA-1 infects Bacillus sp. strain G1R and has a linear double-stranded DNA genome with a terminal protein covalently linked to its 5' ends. GA-1 protein p6 is very abundant in infected cells and binds DNA with no sequence specificity. We show here that it binds in vivo to the whole viral genome, as detect...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Alcorlo M,Salas M,Hermoso JM

    更新日期:2007-11-01 00:00:00

  • New aerobic benzoate oxidation pathway via benzoyl-coenzyme A and 3-hydroxybenzoyl-coenzyme A in a denitrifying Pseudomonas sp.

    abstract::A denitrifying Pseudomonas sp. is able to oxidize aromatic compounds compounds completely to CO2, both aerobically and anaerobically. It is shown that benzoate is aerobically oxidized by a new degradation pathway via benzoyl-coenzyme A (CoA) and 3-hydroxybenzoyl-CoA. The organism grew aerobically with benzoate, 3-hydr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Altenschmidt U,Oswald B,Steiner E,Herrmann H,Fuchs G

    更新日期:1993-08-01 00:00:00

  • Secretion of LamB-LacZ by the signal recognition particle pathway of Escherichia coli.

    abstract::LamB-LacZ fusion proteins have classically been used in studies of the general secretion pathway of Escherichia coli. Here we describe how increasing signal sequence hydrophobicity routes LamB-LacZ Hyb42-1 to the signal recognition particle (SRP) pathway. Secretion of this hydrophobic fusion variant (H*LamB-LacZ) was ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Bowers CW,Lau F,Silhavy TJ

    更新日期:2003-10-01 00:00:00

  • Role of glutamate dehydrogenase in ammonia assimilation in nitrogen-fixing Bacillus macerans.

    abstract::Pathways of ammonia assimilation into glutamic acid in Bacillus macerans were investigated by measurements of the specific activities of glutamate dehydrogenase (GDH), glutamine synthetase, and glutamate synthase. In ammonia-rich medium, GDH was the predominant pathway of ammonia assimilation. In nitrogen-fixing cells...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kanamori K,Weiss RL,Roberts JD

    更新日期:1987-10-01 00:00:00

  • Expression of genes kdsA and kdsB involved in 3-deoxy-D-manno-octulosonic acid metabolism and biosynthesis of enterobacterial lipopolysaccharide is growth phase regulated primarily at the transcriptional level in Escherichia coli K-12.

    abstract::We have cloned and sequenced a cluster of six open reading frames containing gene kdsA from Escherichia coli K-12. The gene encodes 3-deoxy-D-manno-octulosonate 8-phosphate synthetase (KDO-8-phosphate synthetase), which catalyzes formation of 3-deoxy-D-manno-octulosonic acid (KDO), an essential component of enterobact...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Strohmaier H,Remler P,Renner W,Högenauer G

    更新日期:1995-08-01 00:00:00