Abstract:
:Previously we have shown that flagella and the O-specific polysaccharide of lipopolysaccharide play a role in colonization of the potato root by plant growth-promoting Pseudomonas strains WCS374 and WCS358. In this paper, we describe a novel cell surface-exposed structure in Pseudomonas putida WCS358 examined with a specific monoclonal antibody. This cell surface structure appeared to be a polysaccharide, which was accessible to the monoclonal antibody at the outer cell surface. Further study revealed that it does not contain 2-keto-3-deoxyoctonate, heptose, or lipid A, indicating that it is not a second type of lipopolysaccharide. Instead, the polysaccharide shared some characteristics with K antigen described for Escherichia coli. From a series of 49 different soil bacteria tested, only one other potato plant growth-promoting Pseudomonas strain reacted positively with the monoclonal antibody. Mutant cells lacking the novel antigen were efficiently isolated by an enrichment method involving magnetic antibodies. Mutant strains defective in the novel antigen contained normal lipopolysaccharide. One of these mutants was affected in neither its ability to adhere to sterile potato root pieces nor its ability to colonize potato roots. We conclude that the bacterial cell surface of P. putida WCS358 contains at least two different polysaccharide structures. These are the O-specific polysaccharide of lipopolysaccharide, which is relevant for potato root colonization, and the novel polysaccharide, which is not involved in adhesion to or colonization of the potato root.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
de Weger LA,Bloemberg GV,van Wezel T,van Raamsdonk M,Glandorf DC,van Vuurde J,Jann K,Lugtenberg BJdoi
10.1128/jb.178.7.1955-1961.1996subject
Has Abstractpub_date
1996-04-01 00:00:00pages
1955-61issue
7eissn
0021-9193issn
1098-5530journal_volume
178pub_type
杂志文章abstract::The nucleotide sequence of the plasmid-encoded LlaKR2I restriction-modification (R-M) system of Lactococcus lactis subsp. lactis biovar diacetylactis KR2 was determined. This R-M system comprises divergently transcribed endonuclease (llaKR2IR) and methyltransferase (llaKR2IM) genes; located in the intergenic region is...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.22.5844-5854.1998
更新日期:1998-11-01 00:00:00
abstract::Swarmer cell differentiation is a complex process involving the activity of many gene products. In this report, we characterized the genetic locus of Tn5 insertion in each of 12 mutants defective in swarmer cell elongation. The mutations fell into four categories affecting either flagellar biosynthesis or energetics, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.3.823-828.1995
更新日期:1995-02-01 00:00:00
abstract::The general stress response in Alphaproteobacteria was recently described to depend on the alternative sigma factor σ(EcfG), whose activity is regulated by its anti-sigma factor NepR. The response regulator PhyR, in turn, regulates NepR activity in a partner-switching mechanism according to which phosphorylation of Ph...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06006-11
更新日期:2011-12-01 00:00:00
abstract::Studies of mitochondrial biogenesis in yeast have been hampered by a lack of suitable membrane markers in anaerobically grown cells subsequently grown in air. Cytochrome c peroxidase activity and subcellular location was studied to determine whether it would be a useful marker for an analysis of mitochondrial formatio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.4.1225-1235.1967
更新日期:1967-10-01 00:00:00
abstract::Using an oligonucleotide microarray, we searched for previously unrecognized transcription units in intergenic regions in the genome of Bacillus subtilis, with an emphasis on identifying small genes activated during spore formation. Nineteen transcription units were identified, 11 of which were shown to depend on one ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00534-10
更新日期:2010-10-01 00:00:00
abstract::DNA involved in the mobilization of broad-host-range plasmid R1162 was localized to a region of 2.7 kilobases within coordinates 3.4 to 6.1 kilobases on the R1162 map. By examining the transfer properties of plasmids containing cloned fragments of DNA from within this region, we showed that at least four trans-active ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.167.2.703-710.1986
更新日期:1986-08-01 00:00:00
abstract::Spectral analysis indicated the presence of a cytochrome cbb3 oxidase under microaerobic conditions in Azospirillum brasilense Sp7 cells. The corresponding genes (cytNOQP) were isolated by using PCR. These genes are organized in an operon, preceded by a putative anaerobox. The phenotype of an A. brasilense cytN mutant...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.21.5689-5696.1998
更新日期:1998-11-01 00:00:00
abstract::PhoB is the response regulator of the Pho regulon. It is composed of two distinct domains, an N-terminal receiver domain and a C-terminal output domain that binds DNA and interacts with sigma(70) to activate transcription of the Pho regulon. Phosphorylation of the receiver domain is required for activation of the prot...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.23.6592-6597.2000
更新日期:2000-12-01 00:00:00
abstract::The major role of RecA is thought to be in helping repair and restart stalled replication forks. During exponential growth, Bacillus subtilis recA cells exhibited few microscopically observable nucleoid defects. However, the efficiency of plating was about 12% of that of the parent strain. A substantial and additive d...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.9.2963-2968.2001
更新日期:2001-05-01 00:00:00
abstract::Agrobacterium tumefaciens R10 was mutagenized by using the promoter probe transposon Tn5-gusA7, and a library of approximately 5,000 transcriptional fusions was screened for octopine-inducible patterns of gene expression. Twenty-one mutants carrying strongly inducible gusA fusions, 20 of which showed defects in the ca...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.7.1872-1880.1996
更新日期:1996-04-01 00:00:00
abstract::An aryl aldehyde oxidoreductase from Nocardia sp. strain NRRL 5646 was purified 196-fold by a combination of Mono-Q, Reactive Green 19 agarose affinity, and hydroxyapatite chromatographies. The purified enzyme runs as a single band of 140 kDa on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The molecular ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.11.3482-3487.1997
更新日期:1997-06-01 00:00:00
abstract::Schlissel, Harvey J. (The University of Kansas, Lawrence), and C. P. Sword. Conditions affecting transformation of a group H streptococcus. J. Bacteriol. 92:1357-1363. 1966.-A defined transforming medium (DTM) containing buffer and 5 to 10 mug per ml of deoxyribonucleic acid was developed to study the physical and che...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.5.1357-1363.1966
更新日期:1966-11-01 00:00:00
abstract::In a previous search for mutants of Salmonella typhimurium that are defective in heme synthesis, one class that is apparently defective in 5-aminolevulinic acid (ALA) uptake (alu) was found. Here, I describe the characterization of these mutations. The mutations all map to a single locus near 77.5 min on the genetic m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.2.325-331.1993
更新日期:1993-01-01 00:00:00
abstract::Extracellular muramidase-2 of Enterococcus hirae ATCC 9790 was purified to homogeneity by substrate binding, guanidine-HCl extraction, and reversed-phase chromatography. A monoclonal antibody, 2F8, which specifically recognizes muramidase-2, was used to screen a genomic library of E. hirae ATCC 9790 DNA in bacteriopha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.5.1619-1625.1992
更新日期:1992-03-01 00:00:00
abstract::We have developed a method, using laser, optical tweezers and direct microscopic analysis of reproductive potential and membrane integrity, to assess single-cell viability in a stationary-phase Escherichia coli population. It is demonstrated here that a reduction in cell integrity, determined by using the fluorescent ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.19.5551-5555.2000
更新日期:2000-10-01 00:00:00
abstract::Mechanisms of Li+ stimulation of proline transport were studied in cells of Escherichia coli 7 and NR70, a mutant of strain 7 lacking adenosine triphosphatase (EC 3.6.1.3). An electrochemical potential difference of Li+ induced in an inward direction of energy-depleted cells caused a transient uptake of proline depend...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.2.560-564.1979
更新日期:1979-08-01 00:00:00
abstract::The complete sequences of the RhsB and RhsC elements of Escherichia coli K-12 have been determined. These sequence data reveal a new repeated sequence, called H-rpt (Hinc repeat), which is distinct from the Rhs core repetition that is found in all five Rhs elements. H-rpt is found in RhsB, RhsC, and RhsE. Characteriza...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.10.2799-2808.1993
更新日期:1993-05-01 00:00:00
abstract::Domingue, Gerald J. (Tulane University, New Orleans, La.), and William A. Pierce, Jr. Effect of partially purified streptococcal M protein on the in vitro phagocytosis of Streptococcus pyogenes. J. Bacteriol. 89:583-588. 1965.-M protein from Streptococcus pyogenes strain Bailey type 12 was adsorbed onto cells of a glo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.3.583-588.1965
更新日期:1965-03-01 00:00:00
abstract::Siderophore-mediated iron transport in Pseudomonas aeruginosa is dependent upon the cytoplasmic membrane-associated TonB1 energy coupling protein for activity. To assess the functional significance of the various regions of this molecule and to identify functionally important residues, the tonB1 gene was subjected to ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.6.1503-1513.2002
更新日期:2002-03-01 00:00:00
abstract::The interruption of the sblA gene of Streptomyces lividans was previously shown to lead to relief of glucose repression of the normally strongly glucose-repressed alpha-amylase gene. In addition to this relief, an early entry into stationary phase was observed when cells were grown in a minimal medium containing gluco...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00891-06
更新日期:2007-02-01 00:00:00
abstract::Regulation of the katB catalase gene in the anaerobic bacterium Bacteroides fragilis was studied. Northern blot hybridization analyses revealed that katB was transcribed as an approximately 1.6-kb monocistronic mRNA. The levels of katB mRNA increased > 15-fold when anaerobic, mid-logarithmic-phase cultures were expose...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.22.7033-7039.1997
更新日期:1997-11-01 00:00:00
abstract::Serpulina hyodysenteriae, the etiologic agent of swine dysentery, contains complex periplasmic flagella which are composed of multiple class A and class B polypeptides. To examine the role these proteins play in flagellar synthesis, structure, and function and to develop strains which may provide insight into the impo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.20.5959-5970.1995
更新日期:1995-10-01 00:00:00
abstract::Acetosyringone, a phenolic inducer of the virulence (vir) genes of Agrobacterium tumefaciens, inhibited the growth of the nopaline-type strains T37 and C58 incubated under acidic conditions. In the course of a 6-day incubation with acetosyringone, avirulent clones were produced in different proportions by strains T37 ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.17.5676-5685.1992
更新日期:1992-09-01 00:00:00
abstract::Four genes, dra, tpp, drm, and pup, that specify enzymes involved in the catabolism of nucleosides and deoxynucleosides in Escherichia coli are known to be very closely linked in the order dra-tpp-drm-pup. By infecting cells with the phage Mu-1 and isolating low-thymine-requiring derivatives of a strain lacking thymid...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.2.475-484.1975
更新日期:1975-02-01 00:00:00
abstract::Thorne, Curtis B. (Fort Detrick, Frederick, Md.). Transduction in Bacillus subtilis. J. Bacteriol. 83:106-111. 1962.-A bacteriophage, SP-10, isolated from soil carries out general transduction in Bacillus subtilis. Phage propagated on a streptomycin-resistant mutant of the wild-type strain W-23 was capable of transduc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.1.106-111.1962
更新日期:1962-01-01 00:00:00
abstract::A series of studies were performed on a Staphylococcus aureus strain thought to contain a pencillinase plasmid integrated into the host chromosome. Reassociation kinetics analysis of whole-cell deoxyribonucleic acid (DNA) in the presence of pure radioactive plasmid DNA revealed that plasmid-specific sequences were pre...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.3.1424-1428.1975
更新日期:1975-12-01 00:00:00
abstract::The effects of exogenous reducing agents on a number of biological properties of purified Chlamydia trachomatis LGV-434 and Chlamydia psittaci meningopneumonitis elementary bodies (EBs) have been examined in an attempt to identify in vitro correlates of early events in the differentiation of the infectious EB to the r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.161.1.25-31.1985
更新日期:1985-01-01 00:00:00
abstract::Calcium-dependent exonuclease activity was produced by sporulating cells of Bacillus subtilis 168. Nuclease activity was released into the culture medium at approximately the same time as sporulation proteases, and production of these enzymes was tied to DNA replication. Results suggest that nuclease production is a f...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.2.768-770.1982
更新日期:1982-02-01 00:00:00
abstract::The recA441 mutant of Escherichia coli, which has been thought to have thermoinducible constitutive RecA protease activity, is known to have two mutations within recA. We show here that the mutation that alters codon 38 actually confers temperature-independent constitutive protease activity; the second mutation in rec...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.163.1.407-409.1985
更新日期:1985-07-01 00:00:00
abstract::Fine details of the internal and external morphology of Cryptococcus neoformans as seen in ultrathin sections are described and illustrated with electron micrographs. The capsule characteristic of this species contained microfibrils (30 to 40 A in diameter) that appeared to radiate from the cell wall and to coil and i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.3.766-777.1967
更新日期:1967-09-01 00:00:00