Abstract:
:In a previous search for mutants of Salmonella typhimurium that are defective in heme synthesis, one class that is apparently defective in 5-aminolevulinic acid (ALA) uptake (alu) was found. Here, I describe the characterization of these mutations. The mutations all map to a single locus near 77.5 min on the genetic map, which is transcribed counterclockwise. Nutritional tests, genetic and physical mapping, and partial DNA sequence analysis revealed that alu mutants are defective in a periplasmic binding protein-dependent permease that also transports dipeptides, encoded by the dpp operon. The uptake of labeled ALA is defective in dpp mutants and is markedly increased in a strain that has elevated transcription of the dpp locus. Unlabeled L-leucyl-glycine competes with labeled ALA for uptake. In a strain carrying both a dpp-lac operon fusion and a functional copy of the dpp locus, the expression of beta-galactosidase is not induced by ALA, nor, in a hemL mutant, does expression of dpp change substantially during starvation for ALA. The dipeptide permease displays a relaxed substrate specificity that allows transport of the important nonpeptide nutrient ALA, whose structure is closely related to that of glycyl-glycine.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Elliott Tdoi
10.1128/jb.175.2.325-331.1993subject
Has Abstractpub_date
1993-01-01 00:00:00pages
325-31issue
2eissn
0021-9193issn
1098-5530journal_volume
175pub_type
杂志文章abstract::A tandem pair of nearly identical genes from Serpulina hyodysenteriae (B204) were cloned and sequenced. The full open reading frame of one gene and the partial open reading frame of the neighboring gene appear to encode secreted proteins which are homologous to, yet distinct from, the 39-kDa extracytoplasmic protein p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.2.444-448.1998
更新日期:1998-01-01 00:00:00
abstract::By means of electron microscope autoradiography of component cultures of Bacillus subtilis exposed to [(3)H]thymidine-labeled transforming deoxyribonucleic acid competent and noncompetent cells can be distinguished. Competence is not limited to a specific phase of the cell division cycle. With serial section electron ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.118.2.334-341.1974
更新日期:1974-05-01 00:00:00
abstract::We have identified recD mutants of Salmonella typhimurium by their ability to support growth of phage P22 abc (anti-RecBCD) mutants, whose growth is prevented by normal host RecBCD function. As in Escherichia coli, the recD gene of S. typhimurium lies between the recB and argA genes at min 61 of the genetic map. Plasm...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.13.4092-4103.1994
更新日期:1994-07-01 00:00:00
abstract::Glucose uptake by the heterofermentative lactic acid bacterium Oenococcus oeni B1 was studied at the physiological and gene expression levels. Glucose- or fructose-grown bacteria catalyzed uptake of [(14)C]glucose over a pH range from pH 4 to 9, with maxima at pHs 5.5 and 7. Uptake occurred in two-step kinetics in a h...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06038-11
更新日期:2011-12-01 00:00:00
abstract::The pckA gene of Rhizobium meliloti, encoding phosphoenolpyruvate carboxykinase, was isolated from a genomic cosmid library by complementation of the succinate growth phenotype of a Pck- mutant. The gene region was mapped by subcloning and Tn5 insertion mutagenesis. The DNA sequence for a 2-kb region containing the st...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.6.1452-1460.1995
更新日期:1995-03-01 00:00:00
abstract:UNLABELLED:The transmembrane Tsr protein of Escherichia coli mediates chemotactic responses to environmental serine gradients. Serine binds to the periplasmic domain of the homodimeric Tsr molecule, promoting a small inward displacement of one transmembrane helix (TM2). TM2 piston displacements, in turn, modulate the s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00274-15
更新日期:2015-08-01 00:00:00
abstract::The crystal structure of Escherichia coli PdxY, the protein product of the pdxY gene, has been determined to a 2.2-A resolution. PdxY is a member of the ribokinase superfamily of enzymes and has sequence homology with pyridoxal kinases that phosphorylate pyridoxal at the C-5' hydroxyl. The protein is a homodimer with ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.23.8074-8082.2004
更新日期:2004-12-01 00:00:00
abstract::During nitrogen-limited growth, transcription of glnA, which codes for glutamine synthetase, requires sigma 54-RNA polymerase and the phosphorylated from the nitrogen regulator I (NRI; also called NtrC). In cells in which the lac promoter controlled expression of the gene coding for NRI, increasing the intracellular c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.1.179-185.1992
更新日期:1992-01-01 00:00:00
abstract::The metD D-methionine transporter locus of Escherichia coli was identified as the abc-yaeE-yaeC cluster (now renamed metNIQ genes). The abc open reading frame is preceded by tandem MET boxes bracketed by the -10 and -35 boxes of a promoter. The expression driven by this promoter is controlled by the MetJ repressor and...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.17.4930-4932.2002
更新日期:2002-09-01 00:00:00
abstract::Four regulated promoters that direct the transcription of genes (i.e., korA, tra, kilB, and korB) involved in the transfer of the Streptomyces plasmid pIJ101 were isolated following the in vitro fusion of plasmid DNA fragments to a promoterless gene encoding the S. lividans extracellular enzyme beta-galactosidase. Int...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.11.5768-5775.1989
更新日期:1989-11-01 00:00:00
abstract::The vir-type IV secretion system of Agrobacterium is assembled from 12 proteins encoded by the virB operon and virD4. VirB1 is one of the least-studied proteins encoded by the virB operon. Its N terminus is a lytic transglycosylase. The C-terminal third of the protein, VirB1*, is cleaved from VirB1 and secreted to the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00480-07
更新日期:2007-09-01 00:00:00
abstract::Evidence of the presence of messenger ribonucleic acid (mRNA) in dormant spores of Bacillus subtilis has been obtained. The bulk RNA from spores was isolated and labeled in vitro with tritiated dimethyl sulfate. The spore RNA hybridized to 2.4 to 3.2% of the B. subtilis genome. The RNA hybridized to both the complemen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.119.2.514-521.1974
更新日期:1974-08-01 00:00:00
abstract::Anaerobic microbial toluene catabolism is initiated by addition of fumarate to the methyl group of toluene, yielding (R)-benzylsuccinate as first intermediate, which is further metabolized via beta-oxidation to benzoyl-coenzyme A (CoA) and succinyl-CoA. A specific succinyl-CoA:(R)-benzylsuccinate CoA-transferase activ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.14.4288-4295.2001
更新日期:2001-07-01 00:00:00
abstract::The expression of Escherichia coli purR, which encodes the pur regulon repressor protein, is autoregulated. Autoregulation at the level of transcription requires two operator sites, designated purRo1 and purRo2 (O1 and O2). Operator O1 is in the region of DNA between the transcription start site and the site for trans...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.10.5758-5766.1990
更新日期:1990-10-01 00:00:00
abstract:UNLABELLED:Clostridium perfringens type D strains are usually associated with diseases of livestock, and their virulence requires the production of epsilon toxin (ETX). We previously showed (J. Li, S. Sayeed, S. Robertson, J. Chen, and B. A. McClane, PLoS Pathog 7:e1002429, 2011, http://dx.doi.org/10.1371/journal.ppat....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00349-15
更新日期:2015-10-01 00:00:00
abstract::Cefsulodin lyses actively growing Escherichia coli by binding specifically to penicillin-binding proteins (PBPs) 1a and 1b. Recent findings (F. García del Portillo, M. A. de Pedro, D. Joseleau-Petit, and R. D'Ari, J. Bacteriol. 171:4217-4221, 1989) have linked cefsulodin-induced lysis to septation during the first div...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.1.1-5.1991
更新日期:1991-01-01 00:00:00
abstract::The uptake of homologous DNA by Haemophilus influenzae was studied as a function of the proton motive force in completely competent cultures in the pH range of 6 to 8. The composition and magnitude of the proton motive force were varied by using the ionophores valinomycin and nigericin (in the presence of various pota...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.157.3.868-873.1984
更新日期:1984-03-01 00:00:00
abstract::Expression of the tryptophanase (tna) operon of Escherichia coli is regulated by catabolite repression and by tryptophan-induced transcription antitermination at Rho-dependent termination sites in the leader region of the operon. Tryptophan induction is dependent on translation of a short leader peptide coding region,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.5.1774-1779.1997
更新日期:1997-03-01 00:00:00
abstract::Altenbern, Robert A. (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.). Growth of L forms of streptomycin-dependent Proteus mirabilis. J. Bacteriol. 85:619-622. 1963.-A streptomycin-dependent mutant of Proteus mirabilis produces L forms on penicillin-containing medium when dihydrostreptomycin is also p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.3.619-622.1963
更新日期:1963-03-01 00:00:00
abstract:UNLABELLED:Bacteriophytochrome photoreceptors (BphPs) and their cognate response regulators make up two-component signal transduction systems which direct bacteria to mount phenotypic responses to changes in environmental light quality. Most of these systems utilize single-domain response regulators to transduce signal...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00872-15
更新日期:2016-03-31 00:00:00
abstract::Six peptidase activities have been distinguished electrophoretically in cell extracts of Salmonella typhimurium with the aid of a histochemical stain. The activities can also be partially separated by chromatography on diethylaminoethyl-cellulose. These peptidases show overlapping substrate specificities. Mutants (pep...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.1.355-363.1974
更新日期:1974-10-01 00:00:00
abstract::Products of the approximately 9.5-kb virB operon are proposed to direct the export of T-DNA/protein complexes across the Agrobacterium tumefaciens envelope en route to plant cells. The presence of conserved nucleoside triphosphate (NTP)-binding domains in VirB4 and VirB11 suggests that one or both proteins couple ener...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.6.1723-1734.1993
更新日期:1993-03-01 00:00:00
abstract::Antiserum to Aeromonas hydrophila A6 cell envelopes was shown in a previous study (C. Y. F. Wong, G. Mayrhofer, M. W. Heuzenroeder, H. M. Atkinson, D. M. Quinn, and R. L. P. Flower, FEMS Immunol. Med. Microbiol. 15:233-241, 1996) to protect mice against lethal infection by this organism. In this study, colony blot ana...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.11.3397-3403.1997
更新日期:1997-06-01 00:00:00
abstract::Low-copy-number plasmids generally encode a partitioning system to ensure proper segregation after replication. Little is known about partitioning of linear plasmids in Streptomyces. SLP2 is a 50-kb low-copy-number linear plasmid in Streptomyces lividans, which contains a typical parAB partitioning operon. In S. livid...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01192-09
更新日期:2010-01-01 00:00:00
abstract::EspF of enteropathogenic Escherichia coli targets mitochondria and subverts a number of cellular functions. EspF consists of six putative Src homology 3 (SH3) domain binding motifs. In this study we identified sorting nexin 9 (SNX9) as a host cell EspF binding partner protein, which binds EspF via its amino-terminal S...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.8.3110-3115.2006
更新日期:2006-04-01 00:00:00
abstract::The Rhizobium phaseoli recA gene has been cloned by interspecific complementation of the Fec phenotype of bacteriophage lambda. The cloned gene restored the recombination proficiency and conferred resistance to DNA-damaging agents (methyl methanesulfonate and nitrofurantoin) to an Escherichia coli recA mutant. The dir...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.10.3035-3040.1991
更新日期:1991-05-01 00:00:00
abstract::The inner or cytoplasmic membrane fraction of the cell envelope of Escherichia coli was isolated by isopycnic centrifugation on sucrose gradients. The membrane proteins were analyzed by electrophoresis in sodium dodecyl sulfate-polyacrylamide gels (8.5%), and up to 56 bands were resolved. Different preparations gave v...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.117.3.947-953.1974
更新日期:1974-03-01 00:00:00
abstract::Sequencing the insertion sites of 8,865 Tn917 insertions in Enterococcus faecalis strain OG1RF identified a hot spot in the replication terminus region corresponding to 6% of the genome where 65% of the transposons had inserted. In E. faecalis, Tn917 preferentially inserted at a 29-bp consensus sequence centered on TA...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.21.7280-7289.2004
更新日期:2004-11-01 00:00:00
abstract::Klite, P. D. (Middle America Research Unit, Balboa Heights, Canal Zone). Intestinal bacterial flora and transit time of three neotropical bat species. J. Bacteriol. 90:375-379. 1965.-Quantitative studies on the intestinal bacterial flora of three neotropical bat species revealed the following average bacterial populat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.2.375-379.1965
更新日期:1965-08-01 00:00:00
abstract::Escherichia coli lactose permease mediates the proton-driven translocation of galactosides across the cytoplasmic membrane. To define regions important for membrane insertion as well as for biological function, we constructed plasmids encoding different portions of the lactose carrier. Among several lacY deletions, tw...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.9.5374-5381.1990
更新日期:1990-09-01 00:00:00