Abstract:
UNLABELLED:The 2-thiouridine (s(2)U) modification of the wobble position in glutamate, glutamine, and lysine tRNA molecules serves to stabilize the anticodon structure, improving ribosomal binding and overall efficiency of the translational process. Biosynthesis of s(2)U in Escherichia coli requires a cysteine desulfurase (IscS), a thiouridylase (MnmA), and five intermediate sulfur-relay enzymes (TusABCDE). The E. coli MnmA adenylates and subsequently thiolates tRNA to form the s(2)U modification. Bacillus subtilis lacks IscS and the intermediate sulfur relay proteins, yet its genome contains a cysteine desulfurase gene, yrvO, directly adjacent to mnmA. The genomic synteny of yrvO and mnmA combined with the absence of the Tus proteins indicated a potential functionality of these proteins in s(2)U formation. Here, we provide evidence that the B. subtilis YrvO and MnmA are sufficient for s(2)U biosynthesis. A conditional B. subtilis knockout strain showed that s(2)U abundance correlates with MnmA expression, and in vivo complementation studies in E. coli IscS- or MnmA-deficient strains revealed the competency of these proteins in s(2)U biosynthesis. In vitro experiments demonstrated s(2)U formation by YrvO and MnmA, and kinetic analysis established a partnership between the B. subtilis proteins that is contingent upon the presence of ATP. Furthermore, we observed that the slow-growth phenotype of E. coli ΔiscS and ΔmnmA strains associated with s(2)U depletion is recovered by B. subtilis yrvO and mnmA. These results support the proposal that the involvement of a devoted cysteine desulfurase, YrvO, in s(2)U synthesis bypasses the need for a complex biosynthetic pathway by direct sulfur transfer to MnmA. IMPORTANCE:The 2-thiouridine (s(2)U) modification of the wobble position in glutamate, glutamine, and lysine tRNA is conserved in all three domains of life and stabilizes the anticodon structure, thus guaranteeing fidelity in translation. The biosynthesis of s(2)U in Escherichia coli requires seven proteins: the cysteine desulfurase IscS, the thiouridylase MnmA, and five intermediate sulfur-relay enzymes (TusABCDE). Bacillus subtilis and most Gram-positive bacteria lack a complete set of biosynthetic components. Interestingly, the mnmA coding sequence is located adjacent to yrvO, encoding a cysteine desulfurase. In this work, we provide evidence that the B. subtilis YrvO is able to transfer sulfur directly to MnmA. Both proteins are sufficient for s(2)U biosynthesis in a pathway independent of the one used in E. coli.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Black KA,Dos Santos PCdoi
10.1128/JB.02625-14subject
Has Abstractpub_date
2015-06-01 00:00:00pages
1952-62issue
11eissn
0021-9193issn
1098-5530pii
JB.02625-14journal_volume
197pub_type
杂志文章abstract::Treick, R. W. (Indiana University, Bloomington), and W. A. Konetzka. Physiological state of Escherichia coli and the inhibition of deoxyribonucleic acid synthesis by phenethyl alcohol. J. Bacteriol. 88:1580-1584. 1964.-The effects of concentration of phenethyl alcohol (PEA) and the physiological state of the cells on ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.6.1580-1584.1964
更新日期:1964-12-01 00:00:00
abstract::A temperate bacteriophage that determines the expression of enterohemolysin was isolated from Escherichia coli O26 strain C3888. The genetic determinant associated with enterohemolysin production (E-Hly determinant) was cloned from EcoRI-digested bacteriophage DNA in vector plasmid pUC8. pUC8 recombinant plasmid pEO19...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.11.6469-6475.1990
更新日期:1990-11-01 00:00:00
abstract::Cell-free extracts with high nitrogenase activity were prepared by sonic oscillation and French press treatment from the blue-gree alga Anabaena cylindrica. Extracts were prepared from cells grown on a 95% N(2)-5% CO(2) gas mixture followed by a period of nitrogen starvation under an atmosphere of 95% argon-5% CO(2). ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.3.913-917.1971
更新日期:1971-03-01 00:00:00
abstract::The location and extent of the homology between plasmids ColE1 and p15A were determined by analysis of heteroduplexes formed between them as well as with a related plasmid, pBR322, and by hybridization of radioactive deoxyribonucleic acids to restriction fragments of p15A and ColE1. The homology between the plasmids c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.145.3.1305-1309.1981
更新日期:1981-03-01 00:00:00
abstract::Although BBA74 initially was described as a 28-kDa virulence-associated outer-membrane-spanning protein with porin-like function, subsequent studies revealed that it is periplasmic and downregulated in mammalian host-adapted spirochetes. To further elucidate the role of this protein in the Borrelia burgdorferi tick-ma...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01802-08
更新日期:2009-04-01 00:00:00
abstract::A genetic element integrating site specifically into a prokaryotic gene usually carries a copy of the 3' portion of that gene that restores the active gene even as the original is disrupted. A cryptic element in Mesorhizobium loti instead carries a copy of the 5' end of the tRNA gene into which it integrated. This has...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.3.859-860.2002
更新日期:2002-02-01 00:00:00
abstract::During conjugation, a single strand of DNA is cleaved at the origin of transfer (oriT) by the plasmid-encoded relaxase. This strand is then unwound from its complement and transferred in the 5'-to-3' direction, with the 3' end likely extended by rolling-circle replication. The resulting, newly synthesized oriT must th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00818-12
更新日期:2012-10-01 00:00:00
abstract::Polysaccharide-containing material was located by histochemical staining on ultrathin sections of Bacillus cereus and several clostridial species during various phases of growth. These components were located along the cell wall in young vegetative cells and along the developing cortex in sporulating cells. In B. cere...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.98.3.1342-1354.1969
更新日期:1969-06-01 00:00:00
abstract::We have isolated the gene encoding L-allo-threonine aldolase (L-allo-TA) from Aeromonas jandaei DK-39, a pyridoxal 5'-phosphate (PLP)-dependent enzyme that stereospecifically catalyzes the interconversion of L-allo-threonine and glycine. The gene contains an open reading frame consisting of 1,014 nucleotides correspon...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.11.3555-3560.1997
更新日期:1997-06-01 00:00:00
abstract::A new bacteriocin, termed lactococcin A (LCN-A), from Lactococcus lactis subsp. cremoris LMG 2130 was purified and sequenced. The polypeptide contained no unusual amino acids and showed no significant sequence similarity to other known proteins. Only lactococci were killed by the bacteriocin. Of more than 120 L. lacti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.12.3879-3887.1991
更新日期:1991-06-01 00:00:00
abstract::Chlamydiae are widespread Gram-negative pathogens of humans and animals. Salicylidene acylhydrazides, developed as inhibitors of type III secretion system (T3SS) in Yersinia spp., have an inhibitory effect on chlamydial infection. However, these inhibitors also have the capacity to chelate iron, and it is possible tha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01677-14
更新日期:2014-08-15 00:00:00
abstract::We have examined the role of the uvrC gene in UV excision repair by studying incision, excision, repair synthesis, and DNA strand reformation in Escherichia coli mutants made permeable to nucleoside triphosphates by toluene treatment. After irradiation, incisions occur normally in uvrC cells in the presence of nicotin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.137.1.397-408.1979
更新日期:1979-01-01 00:00:00
abstract::The cytoskeletal proteins HMW1 and HMW2 are components of the terminal organelle of the cell wall-less bacterium Mycoplasma pneumoniae. HMW1 is required for a tapered, filamentous morphology but exhibits accelerated turnover in the absence of HMW2. Here, we report that a reciprocal dependency exists between HMW1 and H...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.24.8221-8228.2004
更新日期:2004-12-01 00:00:00
abstract::The potassium, calcium, magnesium, and zinc ion content of cells in exponential and synchronously growing cultures of Escherichia coli B/r was determined with an X-ray fluorescence spectrometer and an atomic absorption spectrophotometer. Cellular potassium, calcium, and magnesium content increased smoothly during the ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.126.3.1089-1095.1976
更新日期:1976-06-01 00:00:00
abstract::The induction of paraffin oxidation in intact cells of Pseudomonas aeruginosa was investigated. Oxidation of (14)C-heptane by cell-free extracts of adapted cells showed that the activity of whole cells is a reliable reflection of the synthesis of the first enzyme in the degradation of n-alkanes. Induction was signific...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.3.706-712.1968
更新日期:1968-09-01 00:00:00
abstract::The effects of exogenous reducing agents on a number of biological properties of purified Chlamydia trachomatis LGV-434 and Chlamydia psittaci meningopneumonitis elementary bodies (EBs) have been examined in an attempt to identify in vitro correlates of early events in the differentiation of the infectious EB to the r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.161.1.25-31.1985
更新日期:1985-01-01 00:00:00
abstract::A novel genetic scheme allowed us to isolate extragenic suppressor mutations that restored mutant OmpF assembly. One group of these mutations, termed asmB for assembly suppressor mutation B, permitted mutant OmpF assembly in a non-allele-specific manner. Genetic mapping analyses placed the asmB mutations at the 2-min ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.17.5138-5143.1996
更新日期:1996-09-01 00:00:00
abstract::The application of mitomycin C induction to 114 genetically diverse Streptococcus agalactiae strains generated 36 phage suspensions. On electron microscopy of the phage suspensions, it was possible to assign the phages to the Siphoviridae family, with three different morphotypes (A, B, and C). Phage genetic diversity ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00426-09
更新日期:2009-08-01 00:00:00
abstract::Weaver, Judith R. (Iowa State University, Ames), and P. A. Pattee. Inducible resistance to erythromycin in Staphylococcus aureus. J. Bacteriol. 88:574-580. 1964.-The dissociated resistance of Staphylococcus aureus to erythromycin was examined and was found to possess the characteristics of an inducible enzyme. The ind...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.3.574-580.1964
更新日期:1964-09-01 00:00:00
abstract:UNLABELLED:The GlnPQ transporter from Lactococcus lactis has the remarkable feature of having two substrate-binding domains (SBDs) fused to the N terminus of the transmembrane domain (TMD), and thus four SBDs are present in the homodimeric complex. Although X-ray structures and ligand binding data are available for bot...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00685-15
更新日期:2015-11-09 00:00:00
abstract::Host range mutants of phage T1 (T1h), which productively infected tonB mutants of Escherichia coli, were isolated. The phage mutants were inactivated by isolated outer membranes of E. coli in contrast to the wild-type phage, which only adsorbed reversibly. For the infection process, the tonB function is apparently onl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.135.1.190-197.1978
更新日期:1978-07-01 00:00:00
abstract::In this study, we report the DNA sequence and biological analysis of a mycobacterial mercury resistance operon encoding a novel Hg(2+) transporter. MerH was found to transport mercuric ions in Escherichia coli via a pair of essential cysteine residues but only when coexpressed with the mercuric reductase. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01063-08
更新日期:2009-01-01 00:00:00
abstract::The accumulation of imidazoleglycerol phosphate during growth of Neurospora crassa in the presence of 3-amino-1,2,4-triazole was found to cause derepression of tryptophan synthetase and to inhibit the induction of kynureninase. Accumulation of indoleglycerol phosphate in response to growth in the presence of indole ac...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.5.1608-1614.1968
更新日期:1968-05-01 00:00:00
abstract::The murG gene of Escherichia coli encodes the N-acetylglucosaminyltransferase responsible for the final step in the formation of the lipid-linked disaccharide-pentapeptide subunit of peptidoglycan. Using trypsin to probe maxicell spheroplasts, we show that this enzyme is peripherally associated with the inner face of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.6.1841-1843.1993
更新日期:1993-03-01 00:00:00
abstract::Clostridium difficile is the etiological agent of antibiotic-associated diarrhea. Among the factors that may play a role in infection are S-layer proteins (SLPs). Previous work has shown these to consist mainly of two components, resulting from the cleavage of a precursor encoded by the slpA gene. The high-molecular-w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.14.3886-3897.2002
更新日期:2002-07-01 00:00:00
abstract::Melanin production is a major virulence factor for Cryptococcus neoformans, an organism causing life-threatening infections in an estimated 10% of AIDS patients. In order to characterize the events involved in melanin synthesis, an enzyme having diphenol oxidase activity was purified and its gene was cloned. The enzym...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.3.656-664.1994
更新日期:1994-02-01 00:00:00
abstract::LaBrec, Eugene H., Herman Schneider, Thomas J. Magnani, and Samuel B. Formal. Epithelial cell penetration as an essential step in the pathogenesis of bacillary dysentery. J. Bacteriol. 88:1503-1518. 1964.-A parent strain of Shigella flexneri 2a and a colonial mutant derived from it were studied in three animal models....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.5.1503-1518.1964
更新日期:1964-11-01 00:00:00
abstract::The crystal structure of Escherichia coli PdxY, the protein product of the pdxY gene, has been determined to a 2.2-A resolution. PdxY is a member of the ribokinase superfamily of enzymes and has sequence homology with pyridoxal kinases that phosphorylate pyridoxal at the C-5' hydroxyl. The protein is a homodimer with ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.23.8074-8082.2004
更新日期:2004-12-01 00:00:00
abstract::The YycFG two-component system is the only signal transduction system in Bacillus subtilis known to be essential for cell viability. This system is highly conserved in low-G+C gram-positive bacteria, regulating important processes such as cell wall homeostasis, cell membrane integrity, and cell division. Four other ge...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01936-06
更新日期:2007-04-01 00:00:00
abstract::The 12 incD repeats in the F plasmid each contain about 60 bits of information, which is three times the amount of conservation that a single protein would need to distinguish the repeats from the rest of the Escherichia coli genome. This is the first reported discovery of a case of threefold excess information, and i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.11.3558-3560.1992
更新日期:1992-06-01 00:00:00