Abstract:
:Chlamydiae are widespread Gram-negative pathogens of humans and animals. Salicylidene acylhydrazides, developed as inhibitors of type III secretion system (T3SS) in Yersinia spp., have an inhibitory effect on chlamydial infection. However, these inhibitors also have the capacity to chelate iron, and it is possible that their antichlamydial effects are caused by iron starvation. Therefore, we have explored the modification of salicylidene acylhydrazides with the goal to uncouple the antichlamydial effect from iron starvation. We discovered that benzylidene acylhydrazides, which cannot chelate iron, inhibit chlamydial growth. Biochemical and genetic analyses suggest that the derivative compounds inhibit chlamydiae through a T3SS-independent mechanism. Four single nucleotide polymorphisms were identified in a Chlamydia muridarum variant resistant to benzylidene acylhydrazides, but it may be necessary to segregate the mutations to differentiate their roles in the resistance phenotype. Benzylidene acylhydrazides are well tolerated by host cells and probiotic vaginal Lactobacillus species and are therefore of potential therapeutic value.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Bao X,Gylfe A,Sturdevant GL,Gong Z,Xu S,Caldwell HD,Elofsson M,Fan Hdoi
10.1128/JB.01677-14subject
Has Abstractpub_date
2014-08-15 00:00:00pages
2989-3001issue
16eissn
0021-9193issn
1098-5530pii
JB.01677-14journal_volume
196pub_type
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journal_title:Journal of bacteriology
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doi:10.1128/jb.177.17.5028-5034.1995
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doi:10.1128/JB.00462-08
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journal_title:Journal of bacteriology
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1994-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1987-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2007-10-01 00:00:00
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更新日期:2011-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1979-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.3.571-574.1965
更新日期:1965-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1973-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2020-06-09 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1979-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1973-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.11.3429-3438.1992
更新日期:1992-06-01 00:00:00