Suppressor analysis reveals a role for SecY in the SecA2-dependent protein export pathway of Mycobacteria.

abstract：:The expression of the positively charged human protein secretory leukocyte protease inhibitor (SLPI) in Escherichia coli causes severe cellular toxicity. After induction of SLPI synthesis in a high-level-expression strain, SGE61, the growth of the strain is arrested and total protein and RNA synthesis rates decline by...

journal_title：Journal of bacteriology

authors： Miller KW,Evans RJ,Eisenberg SP,Thompson RC

更新日期：1989-04-01 00:00:00

abstract：:Vibrio parahaemolyticus possesses two types of flagella, polar and lateral, powered by distinct energy sources, which are derived from the sodium and proton motive forces, respectively. Although proton-powered flagella in Escherichia coli and Salmonella enterica serovar Typhimurium have been extensively studied, the m...

journal_title：Journal of bacteriology

authors： Boles BR,McCarter LL

更新日期：2000-02-01 00:00:00

abstract：:The urease activity of Ureaplasma urealyticum was found to be located in the cytoplasmic fraction and consisted of multiple stable enzyme forms representing at least four biotypes. ...

journal_title：Journal of bacteriology

authors： Delisle GJ

更新日期：1977-06-01 00:00:00

abstract：:Band 7 proteins, which encompass members of the stomatin, prohibitin, flotillin, and HflK/C protein families, are integral membrane proteins that play important physiological roles in eukaryotes but are poorly characterized in bacteria. We have studied the band 7 proteins encoded by the cyanobacterium Synechocystis sp...

journal_title：Journal of bacteriology

authors： Boehm M,Nield J,Zhang P,Aro EM,Komenda J,Nixon PJ

更新日期：2009-10-01 00:00:00

abstract：:Accumulation of certain nonmetabolizable sugar-phosphates (including α-methyl glucoside-6-phosphate) in Escherichia coli is growth inhibitory and elicits the glucose-phosphate stress response. The transcription factor SgrR activates transcription of the small RNA SgrS under stress conditions. SgrS represses translatio...

journal_title：Journal of bacteriology

authors： Sun Y,Vanderpool CK

更新日期：2011-01-01 00:00:00

abstract：:We have cloned fragments of DNA (up to 13 kb), from Pseudomonas putida AJ1, that code for two stereospecific haloalkanoate dehalogenases. These enzymes are highly specific for D and L substrates. The two genes, designated hadD and hadL, have been isolated and independently expressed in Escherichia coli and P. putida h...

journal_title：Journal of bacteriology

authors： Barth PT,Bolton L,Thomson JC

更新日期：1992-04-01 00:00:00

abstract：:Two-micrometer deoxyribonucleic acid (DNA), a circular plasmid of Saccharomyces cerevisiae, contains two nontandem repeated sequences which are inverted with respect to one another. These repeated sequences together account for 21% of the molecule length. Restriction endonuclease analysis and electron microscopy demon...

journal_title：Journal of bacteriology

authors： Livingston DM,Klein HL

更新日期：1977-01-01 00:00:00

abstract：:Auxotrophs of Acinetobacter calcoaceticus blocked in each reaction of the synthetic pathway from chorismic acid to tryptophan were obtained after N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis. One novel class was found to be blocked in both anthranilate and p-aminobenzoate synthesis; these mutants (trpG) require p-...

journal_title：Journal of bacteriology

authors： Sawula RV,Crawford IP

更新日期：1972-11-01 00:00:00

abstract：:Pseudomonas sp. strain PCL1171 displays colony phase variation between opaque phase I and translucent phase II colonies, thereby regulating the production of secondary metabolites and exoenzymes. Complementation and sequence analysis of 26 phase II mutants and of 13 wild-type phase II sectors growing out of phase I co...

journal_title：Journal of bacteriology

authors： van den Broek D,Chin-A-Woeng TF,Bloemberg GV,Lugtenberg BJ

更新日期：2005-01-01 00:00:00

abstract：:Five lacY mutants with amber stop codons at known positions were each placed into 12 different suppressor strains. The 60 amino acid substitutions obtained in this manner were tested for growth on lactose-minimal medium plates and for transport of lactose, melibiose, and thiomethylgalactoside. Most of the amino acid s...

journal_title：Journal of bacteriology

authors： Huang AM,Lee JI,King SC,Wilson TH

更新日期：1992-08-01 00:00:00

abstract：:Eight species representative of the serological diversity of the Mycoplasmatales were tested for their ability to incorporate radiolabeled nucleic acid precursors into acid-insoluble material. Cultures in complex growth medium were centrifuged and resuspended in minimal essential medium (Eagle). For Acholeplasma laidl...

journal_title：Journal of bacteriology

authors： McIvor RS,Kenny GE

更新日期：1978-08-01 00:00:00

abstract：:An important feature of Escherichia coli pathogenesis is an ability to withstand extremely acidic environments of pH 2 or lower. This acid resistance property contributes to the low infectious dose of pathogenic E. coli species. One very efficient E. coli acid resistance system encompasses two isoforms of glutamate de...

journal_title：Journal of bacteriology

authors： Ma Z,Richard H,Tucker DL,Conway T,Foster JW

更新日期：2002-12-01 00:00:00

abstract：:Finnerty, W. R. (University of Iowa, Iowa City), and R. E. Kallio. Origin of palmitic acid carbon in palmitates formed from hexadecane-1-C(14) and tetradecane-1-C(14) by Micrococcus cerificans. J. Bacteriol. 87:1261-1265. 1964.-Degradation of the palmitic acid moiety of cetyl palmitate and myristyl palmitate formed fr...

journal_title：Journal of bacteriology

authors： FINNERTY WR,KALLIO RE

更新日期：1964-06-01 00:00:00

abstract：:The last step in proline biosynthesis in Escherichia coli K-12, Salmonella typhimurium LT7, and a number of other enterobacterial isolates is regulated so that no proline is excreted, even if excess Delta(1)-pyrroline-5-carboxylate, the immediate precursor of proline, is added to a culture. In proline auxotrophs block...

journal_title：Journal of bacteriology

authors： Berg CM,Rossi JJ

更新日期：1974-06-01 00:00:00

abstract：:In Escherichia coli, the frequencies of IS50 and Tn5 transposition are greater in Dam- cells than in isogenic Dam+ cells. IS50 transposition is increased approximately 1,000-fold and Tn5 transposition frequencies are increased about 5- to 10-fold in the absence of Dam methylation. However, in cells that are deficient ...

journal_title：Journal of bacteriology

authors： Makris JC,Nordmann PL,Reznikoff WS

更新日期：1990-03-01 00:00:00

abstract：:The RNA polymerase (RNAP) binding protein A (RbpA) contributes to the formation of stable RNAP-promoter open complexes (RPo) and is essential for viability in mycobacteria. Four domains have been identified in the RbpA protein, i.e., an N-terminal tail (NTT) that interacts with RNAP β' and σ subunits, a core domain (C...

journal_title：Journal of bacteriology

authors： Prusa J,Jensen D,Santiago-Collazo G,Pope SS,Garner AL,Miller JJ,Ruiz Manzano A,Galburt EA,Stallings CL

更新日期：2018-06-11 00:00:00

abstract：:Rhizobia form a symbiotic relationship with plants of the legume family to produce nitrogen-fixing root nodules under nitrogen-limiting conditions. We have examined the importance of glutathione (GSH) during free-living growth and symbiosis of Sinorhizobium meliloti. An S. meliloti mutant strain (SmgshA) which is unab...

journal_title：Journal of bacteriology

authors： Harrison J,Jamet A,Muglia CI,Van de Sype G,Aguilar OM,Puppo A,Frendo P

更新日期：2005-01-01 00:00:00

abstract：:The cysB region in Salmonella typhimurium regulates in a positive manner the noncontiguous structural genes for the enzymes responsible for sulfate reduction in cysteine biosynthesis. We treated three cysB mutants with chemical mutagens and selected 81 secondary mutants in which the inability to utilize sulfate was su...

journal_title：Journal of bacteriology

authors： Borum PR,Monty KJ

更新日期：1976-01-01 00:00:00

abstract：:In cells of Leuconostoc oenos, the fermentation of L-malic acid generates both a transmembrane pH gradient, inside alkaline, and an electrical potential gradient, inside negative. In resting cells, the proton motive force ranged from -170 mV to -88 mV between pH 3.1 and 5.6 in the presence Of L-malate. Membrane potent...

journal_title：Journal of bacteriology

authors： Salema M,Lolkema JS,San Romão MV,Lourero Dias MC

更新日期：1996-06-01 00:00:00

abstract：:The chromosomal location of two genetic loci involved in the transport of cyanocobalamin (B(12)) in Escherichia coli K-12 was determined. One gene, btuA, is believed to code for the transport protein in the cytoplasmic membrane, because a mutant with an alteration in this gene has lost the ability to accumulate B(12) ...

journal_title：Journal of bacteriology

authors： Kadner RJ,Liggins GL

更新日期：1973-08-01 00:00:00

abstract：:During growth of the halophilic archaeon Haloarcula marismortui on D-xylose, a specific D-xylose dehydrogenase was induced. The enzyme was purified to homogeneity. It constitutes a homotetramer of about 175 kDa and catalyzed the oxidation of xylose with both NADP+ and NAD+ as cosubstrates with 10-fold higher affinity ...

journal_title：Journal of bacteriology

authors： Johnsen U,Schönheit P

更新日期：2004-09-01 00:00:00

abstract：:The Escherichia coli gene cluster encoding enzymatic activities responsible for the synthesis and activation of 2,3-dihydroxybenzoic acid in the formation of the catechol siderophore enterobactin was localized to a 4.2-kilobase chromosomal DNA fragment. Analysis of various subclones and transposon insertion mutations ...

journal_title：Journal of bacteriology

authors： Nahlik MS,Fleming TP,McIntosh MA

更新日期：1987-09-01 00:00:00

abstract：:The plasmid-encoded yop genes of pathogenic yersiniae are regulated by the environmental stimuli calcium and temperature. A novel protein, LcrQ, which exhibits a key function in the negative calcium-controlled pathway, was identified. DNA sequence analysis revealed that LcrQ has a molecular mass of 12,412 daltons and ...

journal_title：Journal of bacteriology

authors： Rimpiläinen M,Forsberg A,Wolf-Watz H

更新日期：1992-05-01 00:00:00

abstract：:Pyridine nucleotide-dependent glucose dehydrogenase activity (GPND) is described for the first time in cell-free extracts of certain blue-green algae. When glucose is added to these crude cell extracts, nicotinamide adenine dinucleotide phosphate is reduced at twice the rate as nicotinamide adenine dinucleotide; but e...

journal_title：Journal of bacteriology

authors： Pulich WM,Van Baalen C

更新日期：1973-04-01 00:00:00

abstract：:Leuconostoc mesenteroides subsp. mesenteroides is one of the most predominant lactic acid bacterial groups during kimchi fermentation. Here, we report the complete genome sequence of L. mesenteroides subsp. mesenteroides J18, which was isolated from kimchi. The genome of the strain consists of a 1,896,561-bp chromosom...

journal_title：Journal of bacteriology

authors： Jung JY,Lee SH,Lee SH,Jeon CO

更新日期：2012-02-01 00:00:00

abstract：:The biological activities of Bacillus subtilis ribosomes pretreated with 0.5, 1, or 2 mmp-chloromercuribenzoate (PCMB) were examined. The ribosomes treated with 1 or 2 mm PCMB lost their capacity to synthesize polyphenylalanine as well as the capacity to bind phenylalanyl-transfer ribonucleic acid. On the other hand, ...

journal_title：Journal of bacteriology

authors： Ranu RS,Kaji A

更新日期：1972-10-01 00:00:00

abstract：:FabG performs the NADPH-dependent reduction of β-keto acyl-acyl carrier protein substrates in the elongation cycle of fatty acid synthesis. We report the characterization of a temperature-sensitive mutation (fabGΔ8) in Escherichia colifabG that results from an in-frame 8-amino-acid residue deletion in the α6/α7 subdom...

journal_title：Journal of bacteriology

authors： Srinivas S,Cronan JE

更新日期：2017-04-25 00:00:00

abstract：:Aspartate transcarbamylase is synthesized during exponential growth of Bacillus subtilis and is inactivated when the cells enter the stationary phase. This work is a study of the regulation of aspartate transcarbamylase synthesis during growth and the stationary phase. Using specific immunoprecipitation of aspartate t...

journal_title：Journal of bacteriology

authors： Maurizi MR,Switzer RL

更新日期：1978-09-01 00:00:00

abstract：:A Pediococcus cerevisiae mutant that actively accumulated folate (PteGlu), in contrast to the wild-type, was also found to exhibit changes in the pattern of uptake of 5-methyl-tetrahydrofolate (5-CH3-H4PteGlu) and amethopterin. Most of the 5-CH3-H4PteGlue accumulated through a glucose- and temperature-dependent proces...

journal_title：Journal of bacteriology

authors： Mandelbaum-Shavit F,Grossowicz N

更新日期：1975-08-01 00:00:00

abstract：:Escherichia coli possesses a soluble flavohemoglobin, with an unknown function, encoded by the hmp gene. A monolysogen containing an hmp-lacZ operon fusion was constructed to determine how the hmp promoter is regulated in response to heme ligands (O2, NO) or the presence of anaerobically utilized electron acceptors (n...

journal_title：Journal of bacteriology

authors： Poole RK,Anjum MF,Membrillo-Hernández J,Kim SO,Hughes MN,Stewart V

更新日期：1996-09-01 00:00:00