Molecular nature of spontaneous modifications in gacS which cause colony phase variation in Pseudomonas sp. strain PCL1171.

abstract：:Bacterial uptake of phosphate is usually accomplished via high-affinity transporters that are commonly regulated by two-component systems, which are activated when the concentration of phosphate is low. Mycobacterium smegmatis possesses two such transporters, the widely distributed PstSCAB system and PhnDCE, a transpo...

journal_title：Journal of bacteriology

authors： Gebhard S,Busby JN,Fritz G,Moreland NJ,Cook GM,Lott JS,Baker EN,Money VA

更新日期：2014-10-01 00:00:00

abstract：:We have sequenced the entire region of DNA required for the biosynthesis of CS5 pili from enterotoxigenic Escherichia coli O115:H40 downstream of the major subunit gene, designated csfA (for coli surface factor five A). Five more open reading frames (ORFs) (csfB, csfC, csfE, csfF, and csfD) which are transcribed in th...

journal_title：Journal of bacteriology

authors： Duthy TG,Staendner LH,Manning PA,Heuzenroeder MW

更新日期：1999-09-01 00:00:00

abstract：:DNA microarrays encompassing the entire genome of Yersinia pestis were used to characterize global regulatory changes during steady-state vegetative growth occurring after shift from 26 to 37 degrees C in the presence and absence of Ca2+. Transcriptional profiles revealed that 51, 4, and 13 respective genes and open r...

journal_title：Journal of bacteriology

authors： Motin VL,Georgescu AM,Fitch JP,Gu PP,Nelson DO,Mabery SL,Garnham JB,Sokhansanj BA,Ott LL,Coleman MA,Elliott JM,Kegelmeyer LM,Wyrobek AJ,Slezak TR,Brubaker RR,Garcia E

更新日期：2004-09-01 00:00:00

abstract：:Linker insertion mutagenesis was used to modify the paracrystalline surface layer (S-layer) protein (RsaA) of the gram-negative bacterium Caulobacter crescentus. Eleven unique BamHI linker insertions in the cloned rsaA gene were identified; at the protein level, these linker insertions introduced 4 to 6 amino acids at...

journal_title：Journal of bacteriology

authors： Bingle WH,Nomellini JF,Smit J

更新日期：1997-02-01 00:00:00

abstract：:The Zymomonas mobilis gene (sacA) encoding a protein with sucrase activity has been cloned in Escherichia coli and its nucleotide sequence has been determined. Potential ribosome-binding site and promoter sequences were identified in the region upstream of the gene which were homologous to E. coli and Z. mobilis conse...

journal_title：Journal of bacteriology

authors： Gunasekaran P,Karunakaran T,Cami B,Mukundan AG,Preziosi L,Baratti J

更新日期：1990-12-01 00:00:00

abstract：:We have developed a general method for determining whether a gene of Escherichia coli is essential for viability. The method requires cloned DNA spanning the gene in question and a reasonably detailed genetic and physical map of the cloned segment. Using this information, one constructs a deletion of the target gene i...

journal_title：Journal of bacteriology

authors： Joyce CM,Grindley ND

更新日期：1984-05-01 00:00:00

abstract：:Aeromonas salmonicida subsp. salmonicida, the etiological agent of furunculosis, is an important fish pathogen. We have screened this bacterium with a broad-host-range probe directed against yscV, the gene that encodes the archetype of a highly conserved family of inner membrane proteins found in every known type III ...

journal_title：Journal of bacteriology

authors： Burr SE,Stuber K,Wahli T,Frey J

更新日期：2002-11-01 00:00:00

abstract：:Bacillus brevis HPD31 contains a surface (S)-layer protein, termed the HWP, which forms a hexagonal array in the cell wall. The 5' region of the HWP gene was isolated from a DNA library constructed in bacteriophage vector EMBL3 from a partial BamHI digest of the chromosomal DNA. The 3' region contained in a 2.7-kiloba...

journal_title：Journal of bacteriology

authors： Ebisu S,Tsuboi A,Takagi H,Naruse Y,Yamagata H,Tsukagoshi N,Udaka S

更新日期：1990-03-01 00:00:00

abstract：:The physiological effects of incubation at nonpermissive temperatures of Escherichia coli mutants that carry a temperature-sensitive dnaZ allele [dnaZ(Ts)2016] were examined. The temperature at which the dnaZ(Ts) protein becomes inactivated in vivo was investigated by measurements of deoxyribonucleic acid (DNA) synthe...

journal_title：Journal of bacteriology

authors： Chu H,Malone MM,Haldenwang WG,Walker JR

更新日期：1977-10-01 00:00:00

abstract：:The wild strain of Serratia marcescens rapidly degraded threonine and formed aminoacetone in a medium containing glucose and urea. Extracts of this strain showed high threonine dehydrogenase and "biosynthetic" threonine deaminase activities, but no threonine aldolase activity. Threonine dehydrogenase-deficient strain ...

journal_title：Journal of bacteriology

authors： Komatsubara S,Murata K,Kisumi M,Chibata I

更新日期：1978-08-01 00:00:00

abstract：:The effects of nickel on the expression of hydrogenase in the hydrogen-oxidizing bacterium Alcaligenes latus were studied. In the absence of added nickel, both hydrogenase activity, measured as O2-dependent H2 uptake, and hydrogenase protein, measured in a Western immunoblot, were very low compared with the levels in ...

journal_title：Journal of bacteriology

authors： Doyle CM,Arp DJ

更新日期：1988-09-01 00:00:00

abstract：:The interaction between endonuclease V, the cyclobutane pyrimidine dimer-specific N-glycosylase/abasic lyase from bacteriophage T4, and DNA was investigated by DNase I footprinting methods. The catalytically inactive mutant E23Q was found to interact with a smaller region of DNA at the abasic site analog, tetrahydrofu...

journal_title：Journal of bacteriology

authors： Latham KA,Manuel RC,Lloyd RS

更新日期：1995-09-01 00:00:00

abstract：:Modification of gonococcal deoxyribonucleic acid (DNA) was investigated, and the relationship with endonuclease production was explored. Both chromosomal and plasmid DNA from different gonococcal strains, irrespective of their plasmid content, was poorly cleaved by the restriction endonucleases HaeII, HaeIII, SacII, a...

journal_title：Journal of bacteriology

authors： Norlander L,Davies JK,Hagblom P,Normark S

更新日期：1981-02-01 00:00:00

abstract：:We used primer extension and mutational analysis to identify a promoter upstream of ilvB, the first gene in the ilv-leu operon of Bacillus subtilis. Between the promoter and ilvB, there is a 482-bp leader region which contains a sequence that resembles a factor-independent transcription terminator. In in vitro transcr...

journal_title：Journal of bacteriology

authors： Grandoni JA,Zahler SA,Calvo JM

更新日期：1992-05-01 00:00:00

abstract：:The nitrogen assimilation control protein (NAC) binds to a site within the promoter region of the histidine utilization operon (hutUH) of Klebsiella aerogenes, and NAC bound at this site activates transcription of hutUH. This NAC-binding site was characterized by a combination of random and directed DNA mutagenesis. M...

journal_title：Journal of bacteriology

authors： Pomposiello PJ,Janes BK,Bender RA

更新日期：1998-02-01 00:00:00

abstract：:The ligated loop of the rabbit intestine was investigated as a possible experimental model for the study of Clostridium perfringens food poisoning. The method of preparation of the challenge inoculum was important in determining whether a given strain would provoke a response. When cultures were grown for 4 hr at 37 C...

journal_title：Journal of bacteriology

authors： Duncan CL,Sugiyama H,Strong DH

更新日期：1968-05-01 00:00:00

abstract：:The cheW gene from Escherichia coli has been cloned an inducible promoter, and the effects of the overproduction of the CheW protein on chemotactic behavior and receptor covalent modification have been examined. Plasmids that contain the cheW gene behind a regulatable promoter complement a cheW mutation when the CheW ...

journal_title：Journal of bacteriology

authors： Sanders DA,Mendez B,Koshland DE Jr

更新日期：1989-11-01 00:00:00

abstract：:Bladen, Howard A. (National Institute of Dental Health, Bethesda, Md.), and Edward G. Hampp. Ultrastructure of Treponema microdentium and Borrelia vincentii. J. Bacteriol. 87:1180-1191.-A small oral Treponema (FM) and Borrelia vincentii (N9) were harvested after 3 to 7 days of incubation and either embedded in Vestopa...

journal_title：Journal of bacteriology

authors： Bladen HA,Hampp EG

更新日期：1964-05-01 00:00:00

abstract：:A periplasmic protein essential for the function of the oligopeptide transport system of Salmonella typhimurium was identified. This protein, encoded by the oppA gene, is one of the most abundant proteins in the periplasm and, with an apparent molecular weight of 52,000, is considerably larger than any other known per...

journal_title：Journal of bacteriology

authors： Higgins CF,Hardie MM

更新日期：1983-09-01 00:00:00

abstract：:The kil-kor regulon of the self-transmissible, broad-host-range plasmid RK2 is a unique network with eight coregulated operons. Among the genes encoded by the kil-kor regulon are trfA, which encodes the replication initiator, and several kil loci (kilA, kilB, kilC, and kilE), each of which is lethal to the host cell i...

journal_title：Journal of bacteriology

authors： Thomson VJ,Jovanovic OS,Pohlman RF,Chang CH,Figurski DH

更新日期：1993-04-01 00:00:00

abstract：:In Salmonella enterica, the biosynthetic pathways for the generation of purines and the essential cofactor thiamine pyrophosphate branch after sharing five enzymatic steps. Phosphoribosyl amine (PRA) is the first intermediate in the common portion of the pathway and is generated from phosphoribosylpyrophosphate and gl...

journal_title：Journal of bacteriology

authors： Browne BA,Ramos AI,Downs DM

更新日期：2006-10-01 00:00:00

abstract：:A hydrocarbon-utilizing Brevibacterium which grew into the oil phase of an oil-water system was tested for survival at elevated temperature. Cells suspended in oil and cells that had been resuspended in aqueous solution were tested by placing 1-ml samples of the cell suspension in small test tubes immersed in a contro...

journal_title：Journal of bacteriology

authors： Severance MM,LaRock PA

更新日期：1973-12-01 00:00:00

abstract：:The region of the colicin E1 polypeptide that interacts with immunity protein has been localized to a 168-residue COOH-terminal peptide. This is the length of a proteolytically generated peptide fragment of colicin E1 against which imm+ function can be demonstrated in osmotically shocked cells. The role of particular ...

journal_title：Journal of bacteriology

authors： Bishop LJ,Bjes ES,Davidson VL,Cramer WA

更新日期：1985-10-01 00:00:00

abstract：:Cells of the cyanobacterium Anabaena variabilis starved for phosphate for 3 days took up phosphate at about 100 times the rate of unstarved cells. Kinetic data suggested that a new transport system had been induced by starvation for phosphate. The inducible phosphate transport system was quickly repressed by addition ...

journal_title：Journal of bacteriology

authors： Thiel T

更新日期：1988-03-01 00:00:00

abstract：:The enzyme nicotinamide mononucleotide (NMN) adenylyltransferase (EC 2.7.7.1) catalyzes the synthesis of NAD+ and nicotinic acid adenine dinucleotide. It has been purified to homogeneity from cellular extracts of the thermophilic archaeon Sulfolobus solfataricus. Through a database search, a highly significant match w...

journal_title：Journal of bacteriology

authors： Raffaelli N,Pisani FM,Lorenzi T,Emanuelli M,Amici A,Ruggieri S,Magni G

更新日期：1997-12-01 00:00:00

abstract：:Analysis of rRNA genes in Vibrio fischeri indicates the presence of eight rRNA gene sets in this organism. It was found that the genes for 5S rRNA, 16S rRNA, and 23S rRNA are organized in operons in the following order: 5' end 16S rRNA 23S RNA 5S rRNA 3' end. Although the operons are homologous, they are not identical...

journal_title：Journal of bacteriology

authors： Amikam D,Kuhn J

更新日期：1987-05-01 00:00:00

abstract：:In Escherichia coli, synthesis of sialic acid is not regulated by allosteric inhibition mediated by cytidine 5'-monophospho-N-acetylneuraminic acid (CMP-NeuNAc). Evidence for the lack of metabolic control by feedback inhibition was demonstrated by measuring the intracellular level of sialic acid and CMP-NeuNAc in muta...

journal_title：Journal of bacteriology

authors： Vimr ER,Troy FA

更新日期：1985-11-01 00:00:00

abstract：:Nitrogenase is a functionally constant protein catalyzing N2 reduction, which is found in many phylogenetic lineages of Archaea and Bacteria. A phylogenetic analysis of nif genes may provide insights into the evolution of the bacterial genomes. Moreover, it may be used to study diazotrophic communities, when classical...

journal_title：Journal of bacteriology

authors： Hurek T,Egener T,Reinhold-Hurek B

更新日期：1997-07-01 00:00:00

abstract：:We have used Tn917lacZ to mutagenize the Bacillus subtilis chromosome and have isolated mutants that are defective in chemotaxis and motility. Mapping of the transposon inserts identified two new loci. Mutations in one of these loci generated mutants that had paralyzed flagella. Accordingly, we designate this a mot lo...

journal_title：Journal of bacteriology

authors： Zuberi AR,Ying CW,Parker HM,Ordal GW

更新日期：1990-12-01 00:00:00

abstract：:Thrust for propulsion of flagellated bacteria is generated by rotation of a propeller, the flagellum. The power to drive the polar flagellar rotary motor of Vibrio parahaemolyticus is derived from the transmembrane potential of sodium ions. Force is generated by the motor on coupling of the movement of ions across the...

journal_title：Journal of bacteriology

authors： McCarter LL

更新日期：1994-10-01 00:00:00