Characterization of Vibrio fischeri rRNA operons and subcloning of a ribosomal DNA promoter.

Abstract:

:Analysis of rRNA genes in Vibrio fischeri indicates the presence of eight rRNA gene sets in this organism. It was found that the genes for 5S rRNA, 16S rRNA, and 23S rRNA are organized in operons in the following order: 5' end 16S rRNA 23S RNA 5S rRNA 3' end. Although the operons are homologous, they are not identical with regard to cleavage sites for various restriction endonucleases. A DNA library was constructed, and three ribosomal DNA clones were obtained. One of these clones contained an entire rRNA operon and was used as a source for subcloning. The promoter region which leads to plasmid instability was successfully subcloned into pHG165. The terminator region was subcloned into pBR322.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Amikam D,Kuhn J

doi

10.1128/jb.169.5.2318-2321.1987

subject

Has Abstract

pub_date

1987-05-01 00:00:00

pages

2318-21

issue

5

eissn

0021-9193

issn

1098-5530

journal_volume

169

pub_type

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