Role of the CheW protein in bacterial chemotaxis: overexpression is equivalent to absence.

Abstract:

:The cheW gene from Escherichia coli has been cloned an inducible promoter, and the effects of the overproduction of the CheW protein on chemotactic behavior and receptor covalent modification have been examined. Plasmids that contain the cheW gene behind a regulatable promoter complement a cheW mutation when the CheW protein is produced at low levels. However, when the CheW protein is greatly overproduced in either a wild-type strain or a cheW mutant, chemotaxis is greatly inhibited, cheW null mutant cells swim smoothly as if they were constantly responding to an attractant. Surprisingly, cells in which the CheW protein is overproduced also swim smoothly. The behavioral defect produced by overproduction of the CheW protein does not require the presence of the cheR, cheB, or cheZ gene. Receptor demethylation is also inhibited by overproduction of the CheW protein, as it is by a mutation in the cheW gene or a response to an attractant. In all respects, therefore, overproduction of the CheW protein has the same consequences as does a mutation in the cheW gene or a response to an attractant. A model involving two states of the CheW protein is proposed to explain its role in bacterial chemotaxis.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Sanders DA,Mendez B,Koshland DE Jr

doi

10.1128/jb.171.11.6271-6278.1989

subject

Has Abstract

pub_date

1989-11-01 00:00:00

pages

6271-8

issue

11

eissn

0021-9193

issn

1098-5530

journal_volume

171

pub_type

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