Physiological effects of growth of an Escherichia coli temperature-sensitive dnaZ mutant at nonpermissive temperatures.

abstract：:Transfection of streptococci is reported here for the first time. ...

journal_title：Journal of bacteriology

authors： Parsons CL,Cole RM

更新日期：1973-03-01 00:00:00

abstract：:Penicillin-binding proteins (PBPs), although characterized from several organisms, have so far not been studied in mycobacteria. The present study is the first characterization of a PBP from Mycobacterium smegmatis. The PBP was purified by solubilization of the membranes with Triton X-100 and successive chromatography...

journal_title：Journal of bacteriology

authors： Basu J,Chattopadhyay R,Kundu M,Chakrabarti P

更新日期：1992-07-01 00:00:00

abstract：:A gene (yacK) encoding a putative multicopper oxidase (MCO) was cloned from Escherichia coli, and the expressed enzyme was demonstrated to exhibit phenoloxidase and ferroxidase activities. The purified protein contained six copper atoms per polypeptide chain and displayed optical and electron paramagnetic resonance (E...

journal_title：Journal of bacteriology

authors： Kim C,Lorenz WW,Hoopes JT,Dean JF

更新日期：2001-08-01 00:00:00

abstract：:Neurospora crassa mutants deficient in asparagine synthetase were selected by using the procedure of inositol-less death. Complementation tests among the 100 mutants isolated suggested that their alterations were genetically allelic. Recombination analysis with strain S1007t, an asparagine auxotroph, indicated that th...

journal_title：Journal of bacteriology

authors： MacPhee KG,Nelson RE,Schuster SM

更新日期：1983-10-01 00:00:00

abstract：:High-density tiling microarray and RNA sequencing technologies were used to analyze the transcriptome of the periodontopathogenic bacterium Porphyromonas gingivalis. The compiled P. gingivalis transcriptome profiles were based on total RNA samples isolated from three different laboratory culturing conditions, and the ...

journal_title：Journal of bacteriology

authors： Høvik H,Yu WH,Olsen I,Chen T

更新日期：2012-01-01 00:00:00

abstract：:Vibrio cholerae is the etiologic agent of cholera in humans. Intestinal colonization occurs in a stepwise fashion, initiating with attachment to the small intestinal epithelium. This attachment is followed by expression of the toxin-coregulated pilus, microcolony formation, and cholera toxin (CT) production. We have r...

journal_title：Journal of bacteriology

authors： Jude BA,Martinez RM,Skorupski K,Taylor RK

更新日期：2009-11-01 00:00:00

abstract：:We have used a plasmid-based transcriptional reporter system to examine the transcriptional effects of 33 single point mutations in the box A region (TATA-like sequence) of the Haloferax volcanii tRNA(Lys) promoter. The most pronounced effects on transcriptional efficiency were found when the nucleotides corresponding...

journal_title：Journal of bacteriology

authors： Palmer JR,Daniels CJ

更新日期：1995-04-01 00:00:00

abstract：:Candida albicans is an opportunistic pathogenic yeast which frequently develops resistance to the antifungal agent fluconazole (FCZ) in patients undergoing long-term therapy. FCZ-resistant strains often display a reduced intracellular FCZ accumulation which correlates with the overexpression of the ATP-binding cassett...

journal_title：Journal of bacteriology

authors： Alarco AM,Raymond M

更新日期：1999-02-01 00:00:00

abstract：:The isocitrate lyase from a thermophilic Bacillus is activated about threefold by a variety of salts. Such strong stimulation of activity is not seen with isocitrate lyase from the mesophiles, Bacillus licheniformis, Bacillus megaterium, Escherichia coli, and Aspergillus nidulans. The salt activation is markedly pH-de...

journal_title：Journal of bacteriology

authors： Griffiths MW,Sundaram TK

更新日期：1973-12-01 00:00:00

abstract：:Desulfurococcus kamchatkensis is an anaerobic organotrophic hyperthermophilic crenarchaeon isolated from a terrestrial hot spring. Its genome consists of a single circular chromosome of 1,365,223 bp with no extrachromosomal elements. A total of 1,474 protein-encoding genes were annotated, among which 205 are exclusive...

journal_title：Journal of bacteriology

authors： Ravin NV,Mardanov AV,Beletsky AV,Kublanov IV,Kolganova TV,Lebedinsky AV,Chernyh NA,Bonch-Osmolovskaya EA,Skryabin KG

更新日期：2009-04-01 00:00:00

abstract：:We describe techniques for isolating individual pairs of mating Escherichia coli and observing them under the light microscope. Some pairs achieved close cell-to-cell contact, whereas others remained loosely connected by invisible connections which may be F pili. After 30 min of mating, the pairs were separated and al...

journal_title：Journal of bacteriology

authors： Ou JT,Anderson TF

更新日期：1970-06-01 00:00:00

abstract：:EspF of enteropathogenic Escherichia coli targets mitochondria and subverts a number of cellular functions. EspF consists of six putative Src homology 3 (SH3) domain binding motifs. In this study we identified sorting nexin 9 (SNX9) as a host cell EspF binding partner protein, which binds EspF via its amino-terminal S...

journal_title：Journal of bacteriology

authors： Marchès O,Batchelor M,Shaw RK,Patel A,Cummings N,Nagai T,Sasakawa C,Carlsson SR,Lundmark R,Cougoule C,Caron E,Knutton S,Connerton I,Frankel G

更新日期：2006-04-01 00:00:00

abstract：:The degradation of synthetic compounds requires bacteria to recruit and adapt enzymes from pathways for naturally occurring compounds. Previous work defined the steps in 2,4-dinitrotoluene (2,4-DNT) metabolism through the ring fission reaction. The results presented here characterize subsequent steps in the pathway th...

journal_title：Journal of bacteriology

authors： Johnson GR,Jain RK,Spain JC

更新日期：2002-08-01 00:00:00

abstract：:In Salmonella enterica, the biosynthetic pathways for the generation of purines and the essential cofactor thiamine pyrophosphate branch after sharing five enzymatic steps. Phosphoribosyl amine (PRA) is the first intermediate in the common portion of the pathway and is generated from phosphoribosylpyrophosphate and gl...

journal_title：Journal of bacteriology

authors： Browne BA,Ramos AI,Downs DM

更新日期：2006-10-01 00:00:00

abstract：:Recombinant plasmid pNP5, consisting of plasmid pMB9 on which the uvrB gene is cloned, fully complements for the defects due to chromosomal uvrB mutations in the presence of the amber suppressor sup-6 or supF. Correndonuclease II activity was also completely restored in in UvrB strains containing both plasmid pNP5 and...

journal_title：Journal of bacteriology

authors： Pannekoek H,Noordermeer I,van de Putte P

更新日期：1979-07-01 00:00:00

abstract：:Mutants of Escherichia coli unable to synthesize a functional pyruvate formate-lyase (PFL) are severely impaired in their capacity to grow by glucose fermentation. In a functional complementation assay designed to isolate the pfl gene from Clostridium butyricum, we fortuitously identified a gene that did not encode a ...

journal_title：Journal of bacteriology

authors： Sawers G,Hesslinger C,Muller N,Kaiser M

更新日期：1998-07-01 00:00:00

abstract：:Transcriptomic, metabolomic, physiological, and computational modeling approaches were integrated to gain insight into the mechanisms of antibiotic tolerance in an in vitro biofilm system. Pseudomonas aeruginosa biofilms were grown in drip flow reactors on a medium composed to mimic the exudate from a chronic wound. A...

journal_title：Journal of bacteriology

authors： Stewart PS,White B,Boegli L,Hamerly T,Williamson KS,Franklin MJ,Bothner B,James GA,Fisher S,Vital-Lopez FG,Wallqvist A

更新日期：2019-10-21 00:00:00

abstract：:Staphylococcus aureus responds to changing extracellular environments in part by adjusting its proteome through alterations of transcriptional priorities and selective degradation of the preexisting pool of proteins. In Bacillus subtilis, the proteolytic adaptor protein MecA has been shown to play a role in assisting ...

journal_title：Journal of bacteriology

authors： Donegan NP,Marvin JS,Cheung AL

更新日期：2014-12-01 00:00:00

abstract：:The cell walls isolated from axenically grown leprosy-derived corynebacteria were submitted to various chemical and enzymatic degradations. The glycan strands of the wall peptidoglycan are essentially composed of N-acetylglycosaminyl-N-acetylmuramic acid disaccharide units. Small amounts of N-acetylglycosaminyl-N-glyc...

journal_title：Journal of bacteriology

authors： Janczura E,Leyh-Bouille M,Cocito C,Ghuysen JM

更新日期：1981-02-01 00:00:00

abstract：UNLABELLED:Silica is deposited in and around the spore coat layer of Bacillus cereus, and enhances the spore's acid resistance. Several peptides and proteins, including diatom silaffin and silacidin peptides, are involved in eukaryotic silica biomineralization (biosilicification). Homologous sequence search revealed a ...

journal_title：Journal of bacteriology

authors： Motomura K,Ikeda T,Matsuyama S,Abdelhamid MA,Tanaka T,Ishida T,Hirota R,Kuroda A

更新日期：2015-10-26 00:00:00

abstract：:The effects of variations in growth conditions on the penicillin response of Streptococcus faecium ATCC 9790 were studied. Changes in the growth temperature and medium composition were found to cause striking changes in the bacterial generation time, cellular penicillin sensitivity (minimum inhibitory concentration), ...

journal_title：Journal of bacteriology

authors： Fontana R,Canepari P,Satta G,Coyette J

更新日期：1983-05-01 00:00:00

abstract：:Enzymes of tyrosine biosynthesis (prephenate dehydrogenase and arogenate dehydrogenase) were characterized in 90 species currently classified within the genera Pseudomonas, Xanthomonas, and Alcaligenes. Variation in cofactor specificity and regulatory properties of the dehydrogenase proteins allowed the separation of ...

journal_title：Journal of bacteriology

authors： Byng GS,Whitaker RJ,Gherna RL,Jensen RA

更新日期：1980-10-01 00:00:00

abstract：:Wiley, W. R. (Washington State University, Pullman) and J. L. Stokes. Requirement of an alkaline pH and ammonia for substrate oxidation by Bacillus pasteurii. J. Bacteriol. 84:730-734. 1962-Resting-cell suspensions of Bacillus paseurii require a pH higher than 8.0 and also NH(4) (+) for the oxidation of glycine, alani...

journal_title：Journal of bacteriology

authors： WILEY WR,STOKES JL

更新日期：1962-10-01 00:00:00

abstract：:We designed a selection strategy for the isolation of Escherichia coli mutants exhibiting enhanced protein disulfide isomerase activity. The folding of a variant of tissue plasminogen activator (v-tPA), a protein containing nine disulfide bonds, in the bacterial periplasm is completely dependent on the level of disulf...

journal_title：Journal of bacteriology

authors： Zhan X,Gao J,Jain C,Cieslewicz MJ,Swartz JR,Georgiou G

更新日期：2004-02-01 00:00:00

abstract：:The tetracycline resistance element of plasmid pBR322 partially complements the potassium transport defect of Escherichia coli K-12 mutants having markedly impaired K+ transport. The plasmid increases K+ transport. The Tn10 element does not result in increased transport, demonstrating that the effect is not general fo...

journal_title：Journal of bacteriology

authors： Dosch DC,Salvacion FF,Epstein W

更新日期：1984-12-01 00:00:00

abstract：:Ultrastructural and biochemical studies of a recombinant Treponema pallidum surface antigen designated 4D have been conducted due to its likely biological significance. Electron microscopy demonstrated that the 190-kilodalton (kDa) 4D molecule is an ordered ring structure of 10-nm diameter. The 90-kDa proteinase K-tre...

journal_title：Journal of bacteriology

authors： Fehniger TE,Radolf JD,Lovett MA

更新日期：1986-03-01 00:00:00

abstract：:In many organisms, phosphatase expression and phosphate (P) uptake are coordinately regulated by the Pho regulon. In Myxococcus xanthus P limitation initiates multicellular development, a process associated with changes in phosphatase expression. We sought here to characterize the link between P acquisition and develo...

journal_title：Journal of bacteriology

authors： Whitworth DE,Holmes AB,Irvine AG,Hodgson DA,Scanlan DJ

更新日期：2008-03-01 00:00:00

abstract：:Vibrio cholerae is a natural inhabitant of aquatic ecosystems. Some strains of V. cholerae can colonize the human host and cause cholera, a profuse watery diarrhea. The major pathogenicity factors and virulence regulators of V. cholerae are either encoded in mobile genetic elements acquired in the environment (e.g. pa...

journal_title：Journal of bacteriology

authors： Sakib SN,Reddi G,Almagro-Moreno S

更新日期：2018-03-26 00:00:00

abstract：:We show that both flanking IS256 elements carried by transposon Tn4001 are capable of generating head-to-tail tandem copies and free circular forms, implying that both are active. Our results suggest that the tandem structures arise from dimeric copies of the donor or vector plasmid present in the population by a mech...

journal_title：Journal of bacteriology

authors： Prudhomme M,Turlan C,Claverys JP,Chandler M

更新日期：2002-01-01 00:00:00

abstract：:Fine details of the internal and external morphology of the yeastlike and mycelial phases of the dimorphic fungal pathogen Sporotrichum schenckii as seen in ultrathin sections are described and illustrated by electronphotomicrography. Comparisons of yeastlike phase ultrastructure were made using two different methods ...

journal_title：Journal of bacteriology

authors： Lane JW,Grrison RG,Field MF

更新日期：1969-11-01 00:00:00