Nickel affects expression of the nickel-containing hydrogenase of Alcaligenes latus.

Abstract:

:The effects of nickel on the expression of hydrogenase in the hydrogen-oxidizing bacterium Alcaligenes latus were studied. In the absence of added nickel, both hydrogenase activity, measured as O2-dependent H2 uptake, and hydrogenase protein, measured in a Western immunoblot, were very low compared with the levels in cells induced for hydrogenase in the presence of nickel. Hydrogenase activity and protein levels were dependent on the added nickel concentration and were saturated at 30 nM added Ni2+. The amount of hydrogenase protein in a culture at a given nickel concentration was calculated from the H2 uptake activity of the culture at that Ni2+ concentration. Between 0 and 30 nM added Ni2+, the amount of hydrogenase protein (in nanomoles) was stoichiometric with the amount of added Ni2+. Thus, all of the added Ni2+ could be accounted for in hydrogenase. Between 0 and 50 nM added Ni2+, all the Ni present in the cultures was associated with the cells after 12 h; above 50 nM added Ni2+, some Ni remained in the medium. No other divalent metal cations tested were able to substitute for Ni2+ in the formation of active hydrogenase. We suggest two possible mechanisms for the regulation of hydrogenase activity and protein levels by nickel.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Doyle CM,Arp DJ

doi

10.1128/jb.170.9.3891-3896.1988

subject

Has Abstract

pub_date

1988-09-01 00:00:00

pages

3891-6

issue

9

eissn

0021-9193

issn

1098-5530

journal_volume

170

pub_type

杂志文章
  • Kinetics and catalytic properties of coenzyme A transferase from Peptostreptococcus elsdenii.

    abstract::Coenzyme A (CoA) transferase from Peptostreptococcus elsdenii was purified to homogeneity, and some of its physical and catalytic properties were determined. The native enzyme has a molecular weight of 181,000 and is composed of two alpha subunits (molecular weight, 65,000) and one beta subunit (molecular weight 50,00...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.128.1.372-381.1976

    authors: Schulman M,Valentino D

    更新日期:1976-10-01 00:00:00

  • Regulatory circuitry of the CsrA/CsrB and BarA/UvrY systems of Escherichia coli.

    abstract::The global regulator CsrA (carbon storage regulator) is an RNA binding protein that coordinates central carbon metabolism, activates flagellum biosynthesis and motility, and represses biofilm formation in Escherichia coli. CsrA activity is antagonized by the untranslated RNA CsrB, to which it binds and forms a globula...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.18.5130-5140.2002

    authors: Suzuki K,Wang X,Weilbacher T,Pernestig AK,Melefors O,Georgellis D,Babitzke P,Romeo T

    更新日期:2002-09-01 00:00:00

  • Flexible Hinges in Bacterial Chemoreceptors.

    abstract::Transmembrane bacterial chemoreceptors are extended, rod-shaped homodimers with ligand-binding sites at one end and interaction sites for signaling complex formation and histidine kinase control at the other. There are atomic-resolution structures of chemoreceptor fragments but not of intact, membrane-inserted recepto...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00593-17

    authors: Akkaladevi N,Bunyak F,Stalla D,White TA,Hazelbauer GL

    更新日期:2018-02-07 00:00:00

  • The pneumococcal cell envelope stress-sensing system LiaFSR is activated by murein hydrolases and lipid II-interacting antibiotics.

    abstract::In the Firmicutes, two-component regulatory systems of the LiaSR type sense and orchestrate the response to various agents that perturb cell envelope functions, in particular lipid II cycle inhibitors. In the current study, we found that the corresponding system in Streptococcus pneumoniae displays similar properties ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01489-09

    authors: Eldholm V,Gutt B,Johnsborg O,Brückner R,Maurer P,Hakenbeck R,Mascher T,Håvarstein LS

    更新日期:2010-04-01 00:00:00

  • Natural occurrence of four reversible flagellar phases in cultures of Salmonella mikawashima.

    abstract::Edwards, P. R. (Communicable Disease Center, U.S. Public Health Service, Atlanta, Ga.), R. Sakazaki, and I. Kato. Natural occurrence of four reversible flagellar phases in cultures of Salmonella mikawashima. J. Bacteriol. 84:99-103. 1962-Study of cultures of Salmonella mikawashima isolated from reptiles revealed that ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.84.1.99-103.1962

    authors: EDWARDS PR,SAKAZAKI R,KATO I

    更新日期:1962-07-01 00:00:00

  • CHANGES IN MOUSE PNEUMONITIS AGENT ASSOCIATED WITH DEVELOPMENT OF RESISTANCE TO CHLORTETRACYCLINE.

    abstract::Moulder, James W. (University of Chicago, Chicago, Ill.), Dorothy L. Novosel, and Ilse I. E. Tribby. Changes in mouse pneumonitis agent associated with development of resistance to chlortetracycline. J. Bacteriol. 89:17-22. 1965.-A chlortetracycline-resistant mutant of mouse pneumonitis agent, a member of the psittaco...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.1.17-22.1965

    authors: MOULDER JW,NOVOSEL DL,TRIBBY II

    更新日期:1965-01-01 00:00:00

  • Bacteriophage SPP1 Chu is an alkaline exonuclease in the SynExo family of viral two-component recombinases.

    abstract::Many DNA viruses concatemerize their genomes as a prerequisite to packaging into capsids. Concatemerization arises from either replication or homologous recombination. Replication is already the target of many antiviral drugs, and viral recombinases are an attractive target for drug design, particularly for combinatio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.8.2465-2474.2003

    authors: Vellani TS,Myers RS

    更新日期:2003-04-01 00:00:00

  • Genetic composition of the Bacillus subtilis SOS system.

    abstract::The SOS response in bacteria includes a global transcriptional response to DNA damage. DNA damage is sensed by the highly conserved recombination protein RecA, which facilitates inactivation of the transcriptional repressor LexA. Inactivation of LexA causes induction (derepression) of genes of the LexA regulon, many o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.22.7655-7666.2005

    authors: Au N,Kuester-Schoeck E,Mandava V,Bothwell LE,Canny SP,Chachu K,Colavito SA,Fuller SN,Groban ES,Hensley LA,O'Brien TC,Shah A,Tierney JT,Tomm LL,O'Gara TM,Goranov AI,Grossman AD,Lovett CM

    更新日期:2005-11-01 00:00:00

  • Genetic characterization of Escherichia coli type 1 pilus adhesin mutants and identification of a novel binding phenotype.

    abstract::Five Escherichia coli type 1 pilus mutants that had point mutations in fimH, the gene encoding the type 1 pilus adhesin FimH, were characterized. FimH is a minor component of type 1 pili that is required for the pili to bind and agglutinate guinea pig erythrocytes in a mannose-inhibitable manner. Point mutations were ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.14.4012-4021.2000

    authors: Hamrick TS,Harris SL,Spears PA,Havell EA,Horton JR,Russell PW,Orndorff PE

    更新日期:2000-07-01 00:00:00

  • Rod shape determination by the Bacillus subtilis class B penicillin-binding proteins encoded by pbpA and pbpH.

    abstract::The peptidoglycan cell wall determines the shape and structural integrity of a bacterial cell. Class B penicillin-binding proteins (PBPs) carry a transpeptidase activity that cross-links peptidoglycan strands via their peptide side chains, and some of these proteins are directly involved in cell shape determination. N...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.16.4717-4726.2003

    authors: Wei Y,Havasy T,McPherson DC,Popham DL

    更新日期:2003-08-01 00:00:00

  • Asparaginase II of Saccharomyces cerevisiae: selection of four mutations that cause derepressed enzyme synthesis.

    abstract::A positive selection method was used to isolate four Saccharomyces cerevisiae mutations that cause derepressed synthesis of asparaginase II. The four mutations (and1, and2, and3, and4) were neither closely linked to each other nor linked to previously characterized mutations (asp3, asp6) which cause the complete loss ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.165.1.293-296.1986

    authors: Kamerud JQ,Roon RJ

    更新日期:1986-01-01 00:00:00

  • Novel mutation causing derepression of several enzymes of sulfur metabolism in Neurospora crassa.

    abstract::A group of enzymes of sulfur metabolism (arylsulfatase, cholinesulfatase, and a number of others) are normally repressed in Neurospora crassa by an abundant supply of a "favored" sulfur source such as methionine or inorganic sulfate. A mutant called scon(c) was isolated in which the formation of each of these enzymes ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.109.1.140-151.1972

    authors: Burton EG,Metzenberg RL

    更新日期:1972-01-01 00:00:00

  • Abortive infection mechanisms and prophage sequences significantly influence the genetic makeup of emerging lytic lactococcal phages.

    abstract::In this study, we demonstrated the remarkable genome plasticity of lytic lactococcal phages that allows them to rapidly adapt to the dynamic dairy environment. The lytic double-stranded DNA phage ul36 was used to sequentially infect a wild-type strain of Lactococcus lactis and two isogenic derivatives with genes encod...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01111-06

    authors: Labrie SJ,Moineau S

    更新日期:2007-02-01 00:00:00

  • Different roles of DosS and DosT in the hypoxic adaptation of Mycobacteria.

    abstract::The DosS (DevS) and DosT histidine kinases form a two-component system together with the DosR (DevR) response regulator in Mycobacterium tuberculosis. DosS and DosT, which have high sequence similarity to each other over the length of their amino acid sequences, contain two GAF domains (GAF-A and GAF-B) in their N-ter...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00550-10

    authors: Kim MJ,Park KJ,Ko IJ,Kim YM,Oh JI

    更新日期:2010-10-01 00:00:00

  • Genome sequence of Herbaspirillum sp. strain GW103, a plant growth-promoting bacterium.

    abstract::Herbaspirillum sp. strain GW103 was isolated from rhizosphere soil of the reed Phragmites australis on reclaimed land. Here we report the 5.05-Mb draft genome sequence of the strain, providing bioinformation about the agronomic benefits of this strain, such as multiple traits relevant to plant root colonization and pl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00806-12

    authors: Lee GW,Lee KJ,Chae JC

    更新日期:2012-08-01 00:00:00

  • Role of the sporulation protein BofA in regulating activation of the Bacillus subtilis developmental transcription factor sigmaK.

    abstract::During sporulation, the Bacillus subtilis transcription factor sigmaK is activated by regulated proteolytic processing. I have used a system that facilitates the analysis of the contributions of a modified form of the processing enzyme, SpoIVFB-GFP, and the regulatory proteins BofA and SpoIVFA to the conversion of pro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.17.5384-5388.1999

    authors: Resnekov O

    更新日期:1999-09-01 00:00:00

  • Cell division in a species of Erwinia IV. Metabolic blocks in panothenate biosynthesis and their relationship to inhibition of cell division.

    abstract::Grula, Mary M. (Oklahoma State University, Stillwater) and E. A. Grula. Cell division in a species of Erwinia. IV. Metabolic blocks in pantothenate biosynthesis and their relationship to inhibition of cell division. J. Bacteriol. 83:989-997. 1962.-Four compounds that inhibit cell division in an Erwinia sp., d-serine, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.5.989-997.1962

    authors: GRULA MM,GRULA EA

    更新日期:1962-05-01 00:00:00

  • Sugar transport through maltoporin of Escherichia coli: role of the greasy slide.

    abstract::The lining of the maltodextrin-specific maltoporin (LamB) channel exhibits a string of aromatic residues, the greasy slide, part of which has been shown previously by crystallography to be involved in substrate binding. To probe the functional role of the greasy slide, alanine scanning mutagenesis has been performed o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.11.2994-2999.2002

    authors: Van Gelder P,Dumas F,Bartoldus I,Saint N,Prilipov A,Winterhalter M,Wang Y,Philippsen A,Rosenbusch JP,Schirmer T

    更新日期:2002-06-01 00:00:00

  • Study of tyrosine transfer ribonucleic acid modification in relation to sporulation in Bacillus subtilis.

    abstract::A reversal in the relative amounts of the two major species of tyrosine transfer ribonucleic acid (tRNATyr) (I and II) has been previously observed by others during the development of Bacillus subtilis. These species have been purified by benzoylated diethylaminoethyl-cellulose chromatography and were shown to differ ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.127.1.268-280.1976

    authors: Menichi B,Heyman T

    更新日期:1976-07-01 00:00:00

  • Presence of polyphosphate of low molecular weight in zygomycetes.

    abstract::Polyphosphate of average chain length corresponding to 10 phosphate units was detected in the mycelial extract of zygomycetes. Gel electrophoresis techniques commonly used for the separation and characterization of acidic mucopolysaccharides were successfully used for the detection, purification, and characterization ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.127.3.1408-1413.1976

    authors: Dietrich SM

    更新日期:1976-09-01 00:00:00

  • Identification by genetic suppression of Escherichia coli TolB residues important for TolB-Pal interaction.

    abstract::The Tol-Pal system of Escherichia coli is involved in maintaining outer membrane stability. Mutations in tolQ, tolR, tolA, tolB, or pal genes result in sensitivity to bile salts and the leakage of periplasmic proteins. Moreover, some of the tol genes are necessary for the entry of group A colicins and the DNA of filam...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.3.821-824.2000

    authors: Ray MC,Germon P,Vianney A,Portalier R,Lazzaroni JC

    更新日期:2000-02-01 00:00:00

  • Highly efficient protoplast transformation system for Streptococcus faecalis and a new Escherichia coli-S. faecalis shuttle vector.

    abstract::A highly efficient protoplast transformation system for Streptococcus faecalis has been developed by systematically optimizing different parameters. Up to 10(6) transformants per micrograms of DNA were consistently obtained within 3 days, and cell wall regeneration of protoplasts was virtually 100%. A systematic searc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.165.3.831-836.1986

    authors: Wirth R,An FY,Clewell DB

    更新日期:1986-03-01 00:00:00

  • Iron respiration-driven proton translocation in aerobic bacteria.

    abstract::Washed cell suspensions of Aquaspirillum magnetotacticum MS-1, A. itersonii E12639, Bacillus subtilis 6633, and Escherichia coli CSH27 translocated protons in response to the added oxidant O2 or NO3-, with triphenylmethylphosphonium bromide as the permeant ion. Iron respiration-driven proton translocation was observed...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.167.2.729-731.1986

    authors: Short KA,Blakemore RP

    更新日期:1986-08-01 00:00:00

  • Cloning of Escherichia coli and Pseudomonas aeruginosa phosphomannose isomerase genes and their expression in alginate-negative mutants of Pseudomonas aeruginosa.

    abstract::The phosphomannose isomerase (pmi) gene of Escherichia coli was cloned on a broad-host-range cosmid vector and expressed in Pseudomonas aeruginosa at a low level. Plasmid pAD3, which harbors the E. coli pmi gene, contains a 6.2-kilobase-pair HindIII fragment derived from the chromosome of E. coli. Subcloning produced ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.161.1.249-257.1985

    authors: Darzins A,Nixon LL,Vanags RI,Chakrabarty AM

    更新日期:1985-01-01 00:00:00

  • Intraperiplasmic growth of Bdellovibrio bacteriovorus 109J: N-deacetylation of Escherichia coli peptidoglycan amino sugars.

    abstract::During intraperiplasmic growth of Bdellovibrio bacteriovorus on Escherichia coli, the substrate cell peptidoglycan is extensively modified as it is converted to bdelloplast peptidoglycan. The initially lysozyme-sensitive peptidoglycan of E. coli was rapidly converted to a lysozyme-resistant form. The conversion was du...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.135.3.1008-1014.1978

    authors: Thomashow MF,Rittenberg SC

    更新日期:1978-09-01 00:00:00

  • Aerobic purification of hydrogenase from Rhizobium japonicum by affinity chromatography.

    abstract::We purified active hydrogenase from free-living Rhizobium japonicum by affinity chromatography. The uptake hydrogenase of R. japonicum has been treated previously as an oxygen-sensitive protein. In this purification, however, reducing agents were not added nor was there any attempt to exclude oxygen. In fact, the addi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.166.3.795-800.1986

    authors: Stults LW,Moshiri F,Maier RJ

    更新日期:1986-06-01 00:00:00

  • Characterization of an ADP-ribosyltransferase toxin (AexT) from Aeromonas salmonicida subsp. salmonicida.

    abstract::An ADP-ribosylating toxin named Aeromonas salmonicida exoenzyme T (AexT) in A. salmonicida subsp. salmonicida, the etiological agent of furunculosis in fish, was characterized. Gene aexT, encoding toxin AexT, was cloned and characterized by sequence analysis. AexT shows significant sequence similarity to the ExoS and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.7.1851-1858.2002

    authors: Braun M,Stuber K,Schlatter Y,Wahli T,Kuhnert P,Frey J

    更新日期:2002-04-01 00:00:00

  • The c-type cytochrome OmcA localizes to the outer membrane upon heterologous expression in Escherichia coli.

    abstract::We have functionally produced the outer membrane cytochrome OmcA from Shewanella oneidensis in Escherichia coli. Substrate accessibility experiments indicate that OmcA is surface exposed in an E. coli B strain but not in a K-12 strain. We show that a functional type II secretion system is required for surface localiza...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00395-08

    authors: Donald JW,Hicks MG,Richardson DJ,Palmer T

    更新日期:2008-07-01 00:00:00

  • A 54-kilodalton protein encoded by pBtoxis is required for parasporal body structural integrity in Bacillus thuringiensis subsp. israelensis.

    abstract::Strains of Bacillus thuringiensis such as B. thuringiensis subsp. israelensis (ONR-60A) and B. thuringiensis subsp. morrisoni (PG-14) pathogenic for mosquito larvae produce a complex parasporal body consisting of several protein endotoxins synthesized during sporulation that form an aggregate of crystalline inclusions...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06095-11

    authors: Diaz-Mendoza M,Bideshi DK,Federici BA

    更新日期:2012-03-01 00:00:00

  • Mycoplasma laidlawii in human burns.

    abstract::Mycoplasma laidlawii was recovered from burns from 13 of 52 patients. In most cases, cocci or bacilli were isolated simultaneously. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.98.2.827-828.1969

    authors: Markham JG,Markham NP

    更新日期:1969-05-01 00:00:00