Construction of a modified penicillin-binding protein 2a from methicillin-resistant Staphylococcus aureus and purification by immobilized metal affinity chromatography.

abstract：:A draft genome sequence of Tsukamurella sp., an aerobic bacterium isolated from a human sputum specimen, is described here. A new virus or provirus, TPA4, was characterized. ...

journal_title：Journal of bacteriology

authors： Oulmi L,Gorlas A,Gimenez G,Robert C,Boulahrouf A,Raoult D,Roux V

更新日期：2012-10-01 00:00:00

abstract：:In contrast to canonical phage endolysins, which require holin-mediated disruption of the membrane to gain access to attack the cell wall, signal anchor release (SAR) endolysins are secreted by the host sec system, where they accumulate in an inactive form tethered to the membrane by their N-terminal SAR domains. SAR ...

journal_title：Journal of bacteriology

authors： Kuty GF,Xu M,Struck DK,Summer EJ,Young R

更新日期：2010-11-01 00:00:00

abstract：:Pseudomonas aeruginosa type IV pili (T4P) are virulence factors that promote infection of cystic fibrosis and immunosuppressed patients. As the absence of T4P impairs colonization, they are attractive targets for the development of novel therapeutics. Genes in the pilMNOPQ operon are important for both T4P assembly an...

journal_title：Journal of bacteriology

authors： Tammam S,Sampaleanu LM,Koo J,Manoharan K,Daubaras M,Burrows LL,Howell PL

更新日期：2013-05-01 00:00:00

abstract：:Bacillus megaterium mutants JV-9 and JV-10 are temperature sensitive for initiation of spore germination. At 46 C, they did not lose heat resistance, dipicolinic acid, or absorbance, indicating that the temperature-sensitive blocks are very early in the sequence of initiation reactions. Strain JV-9 was temperature sen...

journal_title：Journal of bacteriology

authors： Vary JC

更新日期：1975-01-01 00:00:00

abstract：:The DosS (DevS) and DosT histidine kinases form a two-component system together with the DosR (DevR) response regulator in Mycobacterium tuberculosis. DosS and DosT, which have high sequence similarity to each other over the length of their amino acid sequences, contain two GAF domains (GAF-A and GAF-B) in their N-ter...

journal_title：Journal of bacteriology

authors： Kim MJ,Park KJ,Ko IJ,Kim YM,Oh JI

更新日期：2010-10-01 00:00:00

abstract：:In Aerobacter aerogenes, the mutational event permitting the utilization of d-arabinose as a source of carbon and energy is a regulatory mutation resulting in the constitutive synthesis of certain enzymes of the l-fucose catabolic pathway. l-Fucose isomerase catalyzes the isomerization of d-arabinose to d-ribulose. Th...

journal_title：Journal of bacteriology

authors： Oliver EJ,Mortlock RP

更新日期：1971-10-01 00:00:00

abstract：:The fruK gene encoding fructose-1-phosphate kinase (FruK), located within the fructose (fru)-catabolic operon of Rhodobacter capsulatus, was sequenced. FruK of R. capsulatus (316 amino acids; molecular weight = 31,232) is the same size as and is homologous to FruK of Escherichia coli, phosphofructokinase B (PfkB) of E...

journal_title：Journal of bacteriology

authors： Wu LF,Reizer A,Reizer J,Cai B,Tomich JM,Saier MH Jr

更新日期：1991-05-01 00:00:00

abstract：:The crystal structure of a small, basic DNA binding protein, Sso10b2, from the thermoacidophilic archaeon Sulfolobus solfataricus was determined by the Zn multiwavelength anomalous diffraction method and refined to 1.85 A resolution. The 89-amino-acid protein adopts a betaalphabetaalphabetabeta topology. The structure...

journal_title：Journal of bacteriology

authors： Chou CC,Lin TW,Chen CY,Wang AH

更新日期：2003-07-01 00:00:00

abstract：:We have identified the transcription start site and regulatory region governing the expression of a sporulation gene in the spoIVC locus of Bacillus subtilis. Efficient expression and developmental regulation of this gene was controlled from a promoter region that extended no more than 110 base pairs upstream and no m...

journal_title：Journal of bacteriology

authors： Kunkel B,Sandman K,Panzer S,Youngman P,Losick R

更新日期：1988-08-01 00:00:00

abstract：:The nucleoids in Mycoplasma capricolum cells were visualized by phase-combined fluorescence microscopy of DAPI (4', 6-diamidino-2-phenylindole)-stained cells. Most growing cells in a rich medium had one or two nucleoids in a cell, and no anucleate cells were found. The nucleoids were positioned in the center in mononu...

journal_title：Journal of bacteriology

authors： Seto S,Miyata M

更新日期：1999-10-01 00:00:00

abstract：:Acinetobacter baumannii has emerged as a significant nosocomial pathogen worldwide. The increasing trend of carbapenem and fluoroquinolone resistance in A. baumannii severely limits the usage of therapeutic antimicrobial agents. Here we report the genome sequence of a multidrug-resistant A. baumannii strain, TCDC-AB07...

journal_title：Journal of bacteriology

authors： Chen CC,Lin YC,Sheng WH,Chen YC,Chang SC,Hsia KC,Liao MH,Li SY

更新日期：2011-05-01 00:00:00

abstract：:The motility of Spirillum volutans is caused by the rotation of each polar flagellar fascicle in a direction opposite to that of the more slowly rotating cell. Both flagella form cones of revolution oriented in the same direction. When the cell reverses its motion, both fascicles simultaneously reverse their rotation ...

journal_title：Journal of bacteriology

authors： Krieg NR,Tomelty JP,Wells JS Jr

更新日期：1967-11-01 00:00:00

abstract：:When cells of Escherichia coli THU were starved for thymine, they continued to grow without division for at least two successive volume doublings at their initial rate. Within experimental error this average rate of volume increase, 0.21 mum(3) per hr, was identical with that observed in control cultures during two ge...

journal_title：Journal of bacteriology

authors： Kubitschek HE

更新日期：1971-02-01 00:00:00

abstract：:The RimM protein in Escherichia coli is associated with free 30S ribosomal subunits but not with 70S ribosomes. A DeltarimM mutant shows a sevenfold-reduced growth rate and a reduced translational efficiency, probably as a result of aberrant assembly of the ribosomal 30S subunits. The slow growth and translational def...

journal_title：Journal of bacteriology

authors： Bylund GO,Lövgren JM,Wikström PM

更新日期：2001-10-01 00:00:00

abstract：:Despite a large number of studies on the role of oxygen in cellular processes, there is no consensus as to how oxygen availability to the cell should be defined, let alone how it should be quantified. Here, a quantitative definition for oxygen availability (perceived aerobiosis) is presented; the definition is based o...

journal_title：Journal of bacteriology

authors： Alexeeva S,Hellingwerf KJ,Teixeira de Mattos MJ

更新日期：2002-03-01 00:00:00

abstract：:Cells of the photosynthetic bacterium Rhodospirillum rubrum cultivated anaerobically in light show phototaxis. The behavior of individual cells in response to the phenomenon is reversal(s) of the swimming direction when the intensity of the light available to them abruptly decreases. The tactic response was inhibited ...

journal_title：Journal of bacteriology

authors： Harayama S,Iino T

更新日期：1977-07-01 00:00:00

abstract：:Numerous field isolates of Moraxella bovis have previously been classified by serological techniques into seven serogroups, each defined by homologous cross-reaction with antisera prepared against purified pili of a single prototype strain. The gene encoding pilin from each of the prototype strains has been characteri...

journal_title：Journal of bacteriology

authors： Atwell JL,Tennent JM,Lepper AW,Elleman TC

更新日期：1994-08-01 00:00:00

abstract：:Sporulation-related repeat (SPOR) domains are small peptidoglycan (PG) binding domains found in thousands of bacterial proteins. The name "SPOR domain" stems from the fact that several early examples came from proteins involved in sporulation, but SPOR domain proteins are quite diverse and contribute to a variety of p...

journal_title：Journal of bacteriology

authors： Yahashiri A,Jorgenson MA,Weiss DS

更新日期：2017-06-27 00:00:00

abstract：:The experiments presented in this paper suggest that the shift observed in sedimentation of deoxyribonucleic acid from cells of Escherichia coli subjected to amino acid starvation is related to inhibition of ribonucleic acid synthesis rather than to its release from the membrane at the termination of replication. ...

journal_title：Journal of bacteriology

authors： Yaffe E,Grossman N,Ron EZ

更新日期：1979-02-01 00:00:00

abstract：:A fusion constructed between the putative attenuator plus the first 219 nucleotides of the ermC (erythromycin resistance) structural gene and a 5' terminally deleted lacZ gene produced a moderate, basal level of beta-galactosidase which was increased by erythromycin addition. Another construction containing an intact ...

journal_title：Journal of bacteriology

authors： Kirsch DR,Lai MH

更新日期：1984-07-01 00:00:00

abstract：:Aggregative amoebae of Dictyostelium discoideum, D. mucoroides, D. purpureum, and D. rosarium react chemotactically to cyclic AMP (cAMP). We measured the chemotactic activity of 14 cAMP analogs and found that these four species have a similar sensitivity to chemical modifications of cAMP; this suggests that the cAMP r...

journal_title：Journal of bacteriology

authors： Van Haastert PJ,Jastorff B,Pinas JE,Konijn TM

更新日期：1982-01-01 00:00:00

abstract：:Clusters of cytoplasmic vesicles resembling those in growing hyphal apices of mycelial fungi are found near the tips of buds in three heterobasidiomycetous yeasts, Rhodotorula glutinis, Candida scottii, and Sporobolomyces salmonicolor. ...

journal_title：Journal of bacteriology

authors： McCully EK,Bracker CE

更新日期：1972-02-01 00:00:00

abstract：:During nitrogen-limited growth, transcription of glnA, which codes for glutamine synthetase, requires sigma 54-RNA polymerase and the phosphorylated from the nitrogen regulator I (NRI; also called NtrC). In cells in which the lac promoter controlled expression of the gene coding for NRI, increasing the intracellular c...

journal_title：Journal of bacteriology

authors： Shiau SP,Schneider BL,Gu W,Reitzer LJ

更新日期：1992-01-01 00:00:00

abstract：:We present the genomic sequence of the human pathogen Legionella pneumophila serogroup 12 strain 570-CO-H (ATCC 43290), a clinical isolate from the Colorado Department of Health, Denver, CO. This is the first example of a genome sequence of L. pneumophila from a serogroup other than serogroup 1. We highlight the simil...

journal_title：Journal of bacteriology

authors： Amaro F,Gilbert JA,Owens S,Trimble W,Shuman HA

更新日期：2012-03-01 00:00:00

abstract：:The smallest viable cell of Spiroplasma citri is a two-turn helix (elementary helix). This elementary helix grows into longer parental cells, which then divide by constriction. The helical morphology is conserved during this process. The growth pattern of S. citri membranes has been investigated by different methods o...

journal_title：Journal of bacteriology

authors： Garnier M,Clerc M,Bové JM

更新日期：1984-04-01 00:00:00

abstract：:Transcription of the ferric citrate transport genes of Escherichia coli is induced by ferric citrate bound to the outer membrane receptor FecA. Additional ferric citrate-specific regulatory proteins are FecR in the cytoplasmic membrane and the FecI sigma factor in the cytoplasm. To further understand the assumed FecR-...

journal_title：Journal of bacteriology

authors： Welz D,Braun V

更新日期：1998-05-01 00:00:00

abstract：:Crude cell extracts of Bacillus subtilis 168T exhibit enzyme activity capable of releasing free uracil from phage PBS1 deoxyribonucleic acid (DNA) in the presence of ethylenediaminetetraacetate. By measuring the enzyme activity in 300 clones that emanated from mutagenized cells, we obtained a mutant strain that did no...

journal_title：Journal of bacteriology

authors：

更新日期：1977-08-01 00:00:00

abstract：:The role of the DNA primase of IncP plasmids was examined with a derivative of RP4 containing Tn7 in the primase gene (pri). The mutant was defective in mediating bacterial conjugation, with the deficiency varying according to the bacterial strains used as donors and recipients. Complementation tests involving recombi...

journal_title：Journal of bacteriology

authors： Merryweather A,Barth PT,Wilkins BM

更新日期：1986-07-01 00:00:00

abstract：:Cell division of Escherichia coli K-12 strain PA3092 was inhibited by the addition of adenosine 3',5'-phosphate (cAMP), and the cellular morphology was changed from rods into filaments. Nucleoids in the filaments were regularly distributed and septum formation was perfectly inhibited. This inhibition of cell division ...

journal_title：Journal of bacteriology

authors： Utsumi R,Tanabe H,Nakamoto Y,Kawamukai M,Sakai H,Himeno M,Komano T,Hirota Y

更新日期：1981-09-01 00:00:00

abstract：:We sequenced the minimum REPI replication region and the incompatibility determinants of pColV-K30. The minimum replication region contains an open reading frame which corresponds to a 35-kilodalton (kDa) protein. Sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis with maxicells transformed with a numb...

journal_title：Journal of bacteriology

authors： Perez-Casal JF,Gammie AE,Crosa JH

更新日期：1989-04-01 00:00:00