Control of cell growth in bacteria: experiments with thymine starvation.

Abstract:

:When cells of Escherichia coli THU were starved for thymine, they continued to grow without division for at least two successive volume doublings at their initial rate. Within experimental error this average rate of volume increase, 0.21 mum(3) per hr, was identical with that observed in control cultures during two generations of growth in the presence of thymine. This growth rate was also independent of the age of the cells at the time of starvation. These results are consistent with the hypothesis, proposed earlier, that growth rates are controlled by uptake sites for binding, transport, or accumulation of compounds into the cell, that the number of these sites is constant throughout most of the cell cycle, and that this number doubles near or at cell division.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Kubitschek HE

doi

10.1128/JB.105.2.472-476.1971

subject

Has Abstract

pub_date

1971-02-01 00:00:00

pages

472-6

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

105

pub_type

杂志文章
  • Location of bacterial polysaccharide during various phases of growth.

    abstract::Polysaccharide-containing material was located by histochemical staining on ultrathin sections of Bacillus cereus and several clostridial species during various phases of growth. These components were located along the cell wall in young vegetative cells and along the developing cortex in sporulating cells. In B. cere...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.98.3.1342-1354.1969

    authors: Walker PD,Short J

    更新日期:1969-06-01 00:00:00

  • asmB, a suppressor locus for assembly-defective OmpF mutants of Escherichia coli, is allelic to envA (lpxC).

    abstract::A novel genetic scheme allowed us to isolate extragenic suppressor mutations that restored mutant OmpF assembly. One group of these mutations, termed asmB for assembly suppressor mutation B, permitted mutant OmpF assembly in a non-allele-specific manner. Genetic mapping analyses placed the asmB mutations at the 2-min ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.17.5138-5143.1996

    authors: Kloser AW,Laird MW,Misra R

    更新日期:1996-09-01 00:00:00

  • Molecular characterization of the gene cluster coxMSL encoding the molybdenum-containing carbon monoxide dehydrogenase of Oligotropha carboxidovorans.

    abstract::The CO dehydrogenase structural genes (cox) and orf4 are clustered in the transcriptional order coxM--> coxS--> coxL--> orf4 on the 128-kb megaplasmid pHCG3 of the carboxidotroph Oligotropha carboxidovorans OM5. Sequence analysis suggested association of molybdopterin cytosine dinucleotide and flavin adenine dinucleot...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.8.2197-2203.1995

    authors: Schübel U,Kraut M,Mörsdorf G,Meyer O

    更新日期:1995-04-01 00:00:00

  • Evolutionary competitiveness of two natural variants of the IncQ-like plasmids, pRAS3.1 and pRAS3.2.

    abstract::Plasmids pRAS3.1 and pRAS3.2 are natural variants of the IncQ-2 plasmid family, that except for two differences, have identical plasmid backbones. Plasmid pRAS3.1 has four 22-bp iterons in its oriV region, while pRAS3.2 has only three 6-bp repeats and pRAS3.1 has five 6-bp repeats in the promoter region of the mobB-mo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00176-10

    authors: Loftie-Eaton W,Rawlings DE

    更新日期:2010-12-01 00:00:00

  • Bioinformatic and biochemical evidence for the identification of the type III secretion system needle protein of Chlamydia trachomatis.

    abstract::Chlamydia spp. express a functional type III secretion system (T3SS) necessary for pathogenesis and intracellular growth. However, certain essential components of the secretion apparatus have diverged to such a degree as to preclude their identification by standard homology searches of primary protein sequences. One e...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01671-07

    authors: Betts HJ,Twiggs LE,Sal MS,Wyrick PB,Fields KA

    更新日期:2008-03-01 00:00:00

  • Proteolytic Cleavage of the Immunodominant Outer Membrane Protein rOmpA in Rickettsia rickettsii.

    abstract::Rickettsia rickettsii, the causative agent of Rocky Mountain spotted fever, contains two immunodominant proteins, rOmpA and rOmpB, in the outer membrane. Both rOmpA and rOmpB are conserved throughout spotted fever group rickettsiae as members of a family of autotransporter proteins. Previously, it was demonstrated tha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00826-16

    authors: Noriea NF,Clark TR,Mead D,Hackstadt T

    更新日期:2017-02-28 00:00:00

  • Effect of glutamate on exogenous citrate catabolism of Neisseria meningitidis and of other species of Neisseria.

    abstract::Resting cell suspensions of Neisseria meningitidis group B (strain 2091) do not catabolize citrate as the sole substrate to an appreciable degree. When another substrate, such as glutamate, is also present to furnish energy for transport, citrate metabolism is greatly stimulated. Within limits, the amount of CO(2) pro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.106.3.819-823.1971

    authors: Hill JC

    更新日期:1971-06-01 00:00:00

  • Transient Intestinal Colonization by a Live-Attenuated Oral Cholera Vaccine Induces Protective Immune Responses in Streptomycin-Treated Mice.

    abstract::Current mouse models for evaluating the efficacy of live oral cholera vaccines (OCVs) have important limitations. Conventionally raised adult mice are resistant to intestinal colonization by Vibrio cholerae, but germfree mice can be colonized and have been used to study OCV immunogenicity. However, germfree animals ha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00232-20

    authors: Fakoya B,Sit B,Waldor MK

    更新日期:2020-11-19 00:00:00

  • Antisense RNA regulation by stable complex formation in the Enterococcus faecalis plasmid pAD1 par addiction system.

    abstract::The par stability determinant, encoded by the Enterococcus faecalis plasmid pAD1, is the only antisense RNA regulated postsegregational killing system identified in gram-positive bacteria. Because of the unique organization of the par locus, the par antisense RNA, RNA II, binds to its target, RNA I, at relatively smal...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.19.6400-6408.2004

    authors: Weaver KE,Ehli EA,Nelson JS,Patel S

    更新日期:2004-10-01 00:00:00

  • Biochemical studies of bacterial sporulation and germination. XII. A sulfonic acid as a major sulfur compound of Bacillus subtilis spores.

    abstract::A sulfonic acid found to be a major constituent of spores of Bacillus subtilis was provisionally identified as 3-l-sulfolactic acid. This compound was completely absent from vegetative cells during growth, but large amounts accumulated in sporulating cells just before the development of refractile spores. Essentially ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.98.1.62-68.1969

    authors: Bonsen PP,Spudich JA,Nelson DL,Kornberg A

    更新日期:1969-04-01 00:00:00

  • RSF1010 plasmid as a potentially useful vector in Pseudomonas species.

    abstract::RSF1010 plasmid DNA was introduced into Pseudomonas putida and P. aeruginosa cells and maintained stably, suggesting the potential usefulness of this plasmid as a vector in Pseudomonas species. The number of copies of RSF1010 was 43 per chromosome equivalent in P. putida cells. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.133.3.1527-1529.1978

    authors: Nagahari K,Sakaguchi K

    更新日期:1978-03-01 00:00:00

  • Protoplast formation and leakage of intramembrane cell components: induction by the competence activator substance of pneumococci.

    abstract::Treatment of pneumococci with activator (a protein that induces bacterial "competence" to absorb deoxyribonucleic acid molecules and undergo genetic transformation) can cause either protoplast formation or leakage of intracellular components to the medium depending on postincubation conditions. The leaked intracellula...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.121.1.344-353.1975

    authors: Seto H,Tomasz A

    更新日期:1975-01-01 00:00:00

  • RNA-Mediated cis Regulation in Acinetobacter baumannii Modulates Stress-Induced Phenotypic Variation.

    abstract::In the nosocomial opportunistic pathogen Acinetobacter baumannii, RecA-dependent mutagenesis, which causes antibiotic resistance acquisition, is linked to the DNA damage response (DDR). Notably, unlike the Escherichia coli paradigm, recA and DDR gene expression in A. baumannii is bimodal. Namely, there is phenotypic v...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00799-16

    authors: Ching C,Gozzi K,Heinemann B,Chai Y,Godoy VG

    更新日期:2017-05-09 00:00:00

  • Identification and mapping of nitrogen fixation genes of Rhodobacter capsulatus: duplication of a nifA-nifB region.

    abstract::Rhodobacter capsulatus mutants unable to fix nitrogen were isolated by random transposon Tn5 mutagenesis. The Tn5 insertion sites of 30 Nif- mutants were mapped within three unlinked chromosomal regions designated A, B, and C. The majority of Tn5 insertions (21 mutants) map within nif region A, characterized by two Cl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.2.693-699.1988

    authors: Klipp W,Masepohl B,Pühler A

    更新日期:1988-02-01 00:00:00

  • Patterns of electrochemical proton gradient formation by membrane vesicles from an obligately acidophilic bacterium.

    abstract::Isolated membrane vesicles from the obligately acidophilic bacterium Bacillus acidocaldarius generated an electrochemical gradient of protons (delta mu- H+) upon energization with ascorbate-phenazine methosulfate at pH 6.0 or 3.0. At pH 6.0, there was little or no transmembrane pH gradient (delta pH), but a transmembr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.159.2.448-452.1984

    authors: Guffanti AA,Mann M,Sherman TL,Krulwich TA

    更新日期:1984-08-01 00:00:00

  • The ToxR protein of Vibrio cholerae forms homodimers and heterodimers.

    abstract::The ToxR protein of Vibrio cholerae regulates the expression of several virulence factors that play important roles in the pathogenesis of cholera. Previous experiments with ToxR-alkaline phosphatase (ToxR-PhoA) fusion proteins suggested a model for gene regulation in which the inactive form of ToxR was a monomer and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.1.156-162.1996

    authors: Ottemann KM,Mekalanos JJ

    更新日期:1996-01-01 00:00:00

  • Isolation and characterization of yeast monomorphic mutants of Candida albicans.

    abstract::A method was devised for the isolation of yeast monomorphic (LEV) mutants of Candida albicans. By this procedure, about 20 stable yeast-like mutants were isolated after mutagenesis with ethyl methane sulfonate. The growth rate of the mutants in different carbon sources, both fermentable and not, was indistinguishable ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.8.2318-2325.1994

    authors: Elorza MV,Sentandreu R,Ruiz-Herrera J

    更新日期:1994-04-01 00:00:00

  • Crystal structure of PhnF, a GntR-family transcriptional regulator of phosphate transport in Mycobacterium smegmatis.

    abstract::Bacterial uptake of phosphate is usually accomplished via high-affinity transporters that are commonly regulated by two-component systems, which are activated when the concentration of phosphate is low. Mycobacterium smegmatis possesses two such transporters, the widely distributed PstSCAB system and PhnDCE, a transpo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01965-14

    authors: Gebhard S,Busby JN,Fritz G,Moreland NJ,Cook GM,Lott JS,Baker EN,Money VA

    更新日期:2014-10-01 00:00:00

  • How many initiator tRNA genes does Escherichia coli need?

    abstract::Multiple copies of a gene require enhanced investment on the part of the cell and, as such, call for an explanation. The observation that Escherichia coli has four copies of initiator tRNA (tRNAi) genes, encoding a special tRNA (tRNA(fMet)) required to start protein synthesis, is puzzling particularly because the cell...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01620-14

    authors: Samhita L,Nanjundiah V,Varshney U

    更新日期:2014-07-01 00:00:00

  • DRS is far less divergent than streptococcal inhibitor of complement of group A streptococcus.

    abstract::When 100 group A streptococcus isolates were screened, drs, a variant of sic, was identified in emm12 and emm55 isolates. Molecular characterization showed that the drs gene sequence is highly conserved, unlike the sic gene sequence. However, the variation in gene size observed was due to the presence of extra interna...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01619-06

    authors: Sagar V,Kumar R,Ganguly NK,Menon T,Chakraborti A

    更新日期:2007-04-01 00:00:00

  • Cloning and nucleotide sequence analysis of the colH gene from Clostridium histolyticum encoding a collagenase and a gelatinase.

    abstract::The colH gene encoding a collagenase was cloned from Clostridium histolyticum JCM 1403. Nucleotide sequencing showed a major open reading frame encoding a 116-kDa protein of 1,021 amino acid residues. The deduced amino acid sequence contains a putative signal sequence and a zinc metalloprotease consensus sequence, HEX...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.21.6489-6496.1994

    authors: Yoshihara K,Matsushita O,Minami J,Okabe A

    更新日期:1994-11-01 00:00:00

  • Physiology of lysine permeases in Saccharomycopsis lipolytica.

    abstract::Two active lysine transport systems were detected in Saccharomycopsis lipolytica. No excretion of lysine out of the cells could be obtained, even by chasing with L-lysine or by poisoning with sodium azide. The kinetic properties of one of the permeases, the high-affinity lysine permease, were studied in detail. Its Km...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.133.2.492-498.1978

    authors: Beckerich JM,Heslot H

    更新日期:1978-02-01 00:00:00

  • The cbb operons of the facultative chemoautotroph Alcaligenes eutrophus encode phosphoglycolate phosphatase.

    abstract::The two highly homologous cbb operons of Alcaligenes eutrophus H16 that are located on the chromosome and on megaplasmid pHG1 contain genes encoding several enzymes of the Calvin carbon reduction cycle. Sequence analysis of a region from the promoter-distal part revealed two open reading frames, designated cbbT and cb...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.22.7329-7340.1993

    authors: Schäferjohann J,Yoo JG,Kusian B,Bowien B

    更新日期:1993-11-01 00:00:00

  • Facilitated diffusion of monosaccharides in Saccharomyces cerevisiae: experimental investigation of kinetic parameters without the assumptions of symmetry.

    abstract::Until the question of symmetry or asymmetry in the facilitated diffusion of monosaccharides by Saccharomyces cerevisiae is resolved, attempts to study the transport process cannot be based on assumptions of symmetry, such as equal concentrations at equilibrium or kinetic parameters that are equal in opposite direction...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.2.612-618.1973

    authors: Kalsow CM,Doyle RJ

    更新日期:1973-02-01 00:00:00

  • Aeromonas salmonicida possesses two genes encoding homologs of the major outer membrane protein, OmpA.

    abstract::Two homologs of the outer membrane protein OmpA were identified in Aeromonas salmonicida by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and amino-terminal sequence analyses. An A. salmonicida genomic DNA library was constructed by using lambda GEM-11 and recombinant phage carrying both g...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.6.1623-1630.1996

    authors: Costello GM,Vipond R,MacIntyre S

    更新日期:1996-03-01 00:00:00

  • Efficient sporulation of yeast in media buffered near pH6.

    abstract::Diploid cells of Saccharomyces cerevisiae underwent meiosis and sporulation when placed in 1% potassium acetate sporulation medium. In unbuffered sporulation medium the pH rose very rapidly, reaching pH 8.4 after 2 h of sporulation. Under these conditions, the uptake of radioactive adenine and lysine was extremely lim...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.132.1.180-185.1977

    authors: McCusker JH,Haber JE

    更新日期:1977-10-01 00:00:00

  • Bioconversion of m-hydroxybenzoate to 2,3-dihydroxybenzoate by mutants of Pseudomonas testosteroni.

    abstract::Mutans of Pseudomonas testosteroni were isolated for their inability to grow on m-hydroxybenzoate as sole carbon source. These mutants hydroxylated m-hydroxybenzoate for form 2,3-dihydroxybenzoate in high yeilds. The bioconversion described in this report represents the first reported example of 3-hydroxybenzoate 2-hy...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.141.1.293-296.1980

    authors: Daumy GO,McColl AS,Andrews GC

    更新日期:1980-01-01 00:00:00

  • Functional analysis of OleY L-oleandrosyl 3-O-methyltransferase of the oleandomycin biosynthetic pathway in Streptomyces antibioticus.

    abstract::Oleandomycin, a macrolide antibiotic produced by Streptomyces antibioticus, contains two sugars attached to the aglycon: L-oleandrose and D-desosamine. oleY codes for a methyltransferase involved in the biosynthesis of L-oleandrose. This gene was overexpressed in Escherichia coli to form inclusion bodies and in Strept...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.183.18.5358-5363.2001

    authors: Rodríguez L,Rodríguez D,Olano C,Braña AF,Méndez C,Salas JA

    更新日期:2001-09-01 00:00:00

  • Activation of silent gal genes in the lac-gal regulon of Streptococcus thermophilus.

    abstract::Streptococcus thermophilus strain CNRZ 302 is unable to ferment galactose, neither that generated intracellularly by lactose hydrolysis nor the free sugar. Nevertheless, sequence analysis and complementation studies with Escherichia coli demonstrated that strain CNRZ 302 contained structurally intact genes for the Lel...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.4.1184-1194.2001

    authors: Vaughan EE,van den Bogaard PT,Catzeddu P,Kuipers OP,de Vos WM

    更新日期:2001-02-01 00:00:00

  • The URE2 protein regulates nitrogen catabolic gene expression through the GATAA-containing UASNTR element in Saccharomyces cerevisiae.

    abstract::Many of the gene products that participate in nitrogen metabolism are sensitive to nitrogen catabolite repression (NCR), i.e., their expression is decreased to low levels when readily used nitrogen sources such as asparagine are provided. Previous work has shown this NCR sensitivity requires the cis-acting UASNTR elem...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.24.7476-7483.1994

    authors: Coffman JA,el Berry HM,Cooper TG

    更新日期:1994-12-01 00:00:00