Abstract:
:We sequenced the minimum REPI replication region and the incompatibility determinants of pColV-K30. The minimum replication region contains an open reading frame which corresponds to a 35-kilodalton (kDa) protein. Sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis with maxicells transformed with a number of deletion derivatives demonstrated that this replication region encodes a 39-kDa protein and also established the direction of transcription of the RepI protein gene. The 39-kDa polypeptide was identified as the trans-acting factor essential for replication of REPI-containing plasmids. A translated region of the nucleotide sequence of the RepI protein gene showed homology with the helix-turn-helix binding domains of a number of DNA-binding proteins and also with other plasmid replication proteins. Further nucleotide analysis of the REPI region revealed the presence of direct and inverted repeat sequences in the incE, incF, and ori regions. The REPI ori also contained a perfect DnaA-binding site in addition to a high frequency of occurrence of the DNA adenine methylation (dam) site 5'GATC3'.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Perez-Casal JF,Gammie AE,Crosa JHdoi
10.1128/jb.171.4.2195-2201.1989subject
Has Abstractpub_date
1989-04-01 00:00:00pages
2195-201issue
4eissn
0021-9193issn
1098-5530journal_volume
171pub_type
杂志文章abstract::The predominant mode of growth of bacteria in the environment is within sessile, matrix-enclosed communities known as biofilms. Biofilms often complicate chronic and difficult-to-treat infections by protecting bacteria from the immune system, decreasing antibiotic efficacy, and dispersing planktonic cells to distant b...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.1.290-301.2002
更新日期:2002-01-01 00:00:00
abstract::Endonuclease V is specific for single-stranded DNA or for duplex DNA that contains uracil or that is damaged by a variety of agents (B. Demple and S. Linn, J. Biol. Chem. 257:2848-2855, 1982). Thus, it may be a versatile DNA repair enzyme. The protein was purified to apparent homogeneity, and from its N-terminal seque...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.2.310-316.1997
更新日期:1997-01-01 00:00:00
abstract::Growth of Schizosaccharomyces pombe in batch cultures in a commercial defined medium was found to be normal until the last generation before the stationary phase. Approximately one-half the progeny of the last division lysed. Lysis occurred at breaks in the cell wall at cell-plates whose fission was eccentric. Normal ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.1.192-195.1967
更新日期:1967-07-01 00:00:00
abstract::Here, we report the draft genome sequence of Antarctic sea ice bacterium Glaciecola punicea ACAM 611(T), the type species of the genus Glaciecola. A blue-light-absorbing proteorhodopsin gene is present in the 3.08-Mb genome. This genome sequence can facilitate the study of the physiological metabolisms and ecological ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00463-12
更新日期:2012-06-01 00:00:00
abstract::Five Spirochaeta aurantia chemotaxis mutants were isolated. One mutant (the che-101 mutant) never reversed, one (the che-200 mutant) flexed predominantly, two (the che-300 and che-400-1 mutants) exhibited elevated reversal frequencies, and one (the che-400 mutant) exhibited chemotactically unstimulated behavior simila...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.1.606-611.1989
更新日期:1989-01-01 00:00:00
abstract::Tn4551, a clindamycin resistance (Ccr) transposon from the R plasmid pBI136, was cloned onto an Escherichia coli-Bacteroides shuttle vector which could replicate normally in E. coli but was maintained unstably in Bacteroides fragilis. To aid in cloning and to ensure maintenance of Tn4551 in E. coli, a kanamycin resist...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.8.3450-3457.1987
更新日期:1987-08-01 00:00:00
abstract::The structural properties required for the binding of peptide substrates to the Escherichia coli periplasmic protein involved in oligopeptide transport were surveyed by measuring the ability of different peptides to compete for binding in an equilibrium dialysis assay with the tripeptide Ala-Phe-[3H]Gly. The protein s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.2.775-779.1986
更新日期:1986-11-01 00:00:00
abstract::The fermentative alcohol dehydrogenase of Escherichia coli is encoded by the adhE gene, which is induced under anaerobic conditions but repressed in air. Previous work suggested that induction of adhE might depend on NADH levels. We therefore directly measured the NAD+ and NADH levels for cultures growing aerobically ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.20.6013-6018.1996
更新日期:1996-10-01 00:00:00
abstract::In this study, we cloned the Pseudomonas aeruginosa zwf gene, encoding glucose-6-phosphate dehydrogenase (G6PDH), an enzyme that catalyzes the NAD+- or NADP+-dependent conversion of glucose-6-phosphate to 6-phosphogluconate. The predicted zwf gene product is 490 residues, which could form a tetramer with a molecular m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.7.1741-1749.1998
更新日期:1998-04-01 00:00:00
abstract::The 5 to 10 peritrichously inserted complex flagella of Rhizobium meliloti MVII-1 were found to form right-handed flagellar bundles. Bacteria swam at speeds up to 60 microns/s, their random three-dimensional walk consisting of straight runs and quick directional changes (turns) without the vigorous angular motion (tum...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.7.3146-3150.1987
更新日期:1987-07-01 00:00:00
abstract::The application of a simple negative staining technique and freeze-etching has revealed detailed information on the species-specific surface structure of spores and aerial hyphae of streptomycetes. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.101.1.318-322.1970
更新日期:1970-01-01 00:00:00
abstract::Treatment of pneumococci with activator (a protein that induces bacterial "competence" to absorb deoxyribonucleic acid molecules and undergo genetic transformation) can cause either protoplast formation or leakage of intracellular components to the medium depending on postincubation conditions. The leaked intracellula...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.1.344-353.1975
更新日期:1975-01-01 00:00:00
abstract::A second synchrony induction treatment, half-way through the deoxyribonucleic acid replication process of an already synchronized culture, resulted in defective subsequent cell growth. This supports a previous explanation for the partial loss of viability after synchrony induction. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.107.2.583-584.1971
更新日期:1971-08-01 00:00:00
abstract::Galactoside permease-containing cells of Escherichia coli can be depleted of energy reserves so that the "downhill" cellular hydrolysis of o-nitrophenyl-beta-d-galactopyranoside (ONPG) no longer takes place. Treatment of such energy-depleted cells with proton-conducting agents such as carbonylcyanide m-chlorophenylhyd...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.123.1.187-195.1975
更新日期:1975-07-01 00:00:00
abstract::The heat shock response of Bradyrhizobium japonicum is controlled by a complex network involving two known regulatory systems. While some heat shock genes are controlled by a highly conserved inverted-repeat structure (CIRCE), others depend on a sigma 32-type heat shock sigma factor. Using Western blot (immunoblot) an...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.18.5337-5346.1996
更新日期:1996-09-01 00:00:00
abstract::The region of the colicin E1 polypeptide that interacts with immunity protein has been localized to a 168-residue COOH-terminal peptide. This is the length of a proteolytically generated peptide fragment of colicin E1 against which imm+ function can be demonstrated in osmotically shocked cells. The role of particular ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.1.237-244.1985
更新日期:1985-10-01 00:00:00
abstract::The nucleoids in Mycoplasma capricolum cells were visualized by phase-combined fluorescence microscopy of DAPI (4', 6-diamidino-2-phenylindole)-stained cells. Most growing cells in a rich medium had one or two nucleoids in a cell, and no anucleate cells were found. The nucleoids were positioned in the center in mononu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.19.6073-6080.1999
更新日期:1999-10-01 00:00:00
abstract::Lag is a temporary period of nonreplication seen in bacteria that are introduced to new media. Despite latency being described by Müller in 1895, only recently have we gained insights into the cellular processes characterizing lag phase. This review covers literature to date on the transcriptomic, proteomic, metabolom...
journal_title:Journal of bacteriology
pub_type: 杂志文章,评审
doi:10.1128/JB.00697-18
更新日期:2019-03-13 00:00:00
abstract::Yeast cells growing on mineral medium plus ammonia and glucose contained high levels of nicotinamide adenine dinucleotide phosphate-dependent glutamate dehydrogenase activity, as measured in crude extracts. After suspension of cells in fresh medium lacking glucose, there was a loss of the glutamate dehydrogenase activ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.133.2.780-785.1978
更新日期:1978-02-01 00:00:00
abstract:UNLABELLED:CbrA is an atypical sensor kinase found in Pseudomonas. The autokinase domain is connected to a putative transporter of the sodium/solute symporter family (SSSF). CbrA functions together with its cognate response regulator, CbrB, and plays an important role in nutrient acquisition, including regulation of hu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00361-15
更新日期:2015-09-01 00:00:00
abstract::The spores of Bacillus subtilis show remarkable resistance to many environmental stresses, due in part to the presence of an outer proteinaceous structure known as the spore coat. GerQ is a spore coat protein essential for the presence of CwlJ, an enzyme involved in the hydrolysis of the cortex during spore germinatio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.17.5567-5575.2004
更新日期:2004-09-01 00:00:00
abstract::The chemical properties of the cell wall of Mycobacterium lepraemurium strain Hawaii were investigated. Five subunits of the cell wall, arabinose mycolate, mycolic acids, tetrapeptide (Ala-Gln-diaminopimelic acid-Ala), disaccharide (N-acetylglucosaminyl-beta-1,4-N-glycolylmuramic acid), and arabinogalactan, were obtai...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.113.1.515-518.1973
更新日期:1973-01-01 00:00:00
abstract::Coscarelli, Waldimero (Rutgers, The State University, New Brunswick, N.J.) and David Pramer. Nutrition and growth of Arthrobotrys conoides. J. Bacteriol. 84:60-64. 1962-Despite its ability to capture, kill, and subsist on nematodes, the predacious fungus Arthrobotrys conoides does not demonstrate a unique nutritional ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.1.60-64.1962
更新日期:1962-07-01 00:00:00
abstract::Fructose-6-phosphate kinase (pfkA) mutants have impaired growth on carbon sources which enter glycolysis at or above the level of fructose-6-phosphate, but the degree of impairment depends on the carbon source (e.g., growth on glucose is very much slower than growth on glucose-6-phosphate). The present report contains...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.118.3.1090-1100.1974
更新日期:1974-06-01 00:00:00
abstract::A widely distributed family of small regulators, called C proteins, controls a subset of restriction-modification systems. The C proteins studied to date activate transcription of their own genes and that of downstream endonuclease genes; this arrangement appears to delay endonuclease expression relative to that of th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.2.488-497.2005
更新日期:2005-01-01 00:00:00
abstract::The disaccharide trehalose is accumulated as a storage product by spores of Streptomyces griseus. Nongerminating spores used their trehalose reserves slowly when incubated in buffer for several months. In contrast, spores rapidly depleted their trehalose pools during the first hours of germination. Extracts of dormant...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.11.5002-5007.1987
更新日期:1987-11-01 00:00:00
abstract::A partial nucleotide sequence that included 1,693 base pairs of the M12 (emm12) gene of group A streptococci (strain CS24) and adjacent upstream DNA was determined. Type 12 M protein-specific mRNA of strain CS24 is transcribed from two promoters (P1 and P3) separated by 30 bases. The transcription start sites of the e...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.12.5633-5640.1987
更新日期:1987-12-01 00:00:00
abstract::Production of deoxyribonucleotides for DNA synthesis is an essential and tightly regulated process. The class Ia ribonucleotide reductase (RNR), the product of the nrdAB genes, is required for aerobic growth of Escherichia coli. In catalyzing the reduction of ribonucleotides, two of the cysteines of RNR become oxidize...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05989-11
更新日期:2012-03-01 00:00:00
abstract::Netropsin, a polypeptide antibiotic which binds specifically to adenylate-thymidylate-rich regions of deoxyribonucleic acid, inhibitis sporulation at about stage II, but does not inhibit growth of Bacillys subtilis. An analysis of the sporulation-associated enzymes aconitase, alkaline phosphatase, and glucose dehydrog...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.128.1.80-85.1976
更新日期:1976-10-01 00:00:00
abstract::Defects in DNA mismatch repair (MMR) occur frequently in natural populations of pathogenic and commensal bacteria, resulting in a mutator phenotype. We identified a unique genetic element in Streptococcus pyogenes strain SF370 that controls MMR via a dynamic process of prophage excision and reintegration in response t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01569-07
更新日期:2008-10-01 00:00:00