Phenotypic suppression of phosphofructokinase mutations in Escherichia coli by constitutive expression of the glyoxylate shunt.

abstract：:In contrast to canonical phage endolysins, which require holin-mediated disruption of the membrane to gain access to attack the cell wall, signal anchor release (SAR) endolysins are secreted by the host sec system, where they accumulate in an inactive form tethered to the membrane by their N-terminal SAR domains. SAR ...

journal_title：Journal of bacteriology

authors： Kuty GF,Xu M,Struck DK,Summer EJ,Young R

更新日期：2010-11-01 00:00:00

abstract：:The addition of 1 mM orthophenanthroline (OP) into a mating mixture drastically reduced the production of recombinants. Examination of the effect of OP on each step of conjugation showed that the effect on the following steps could not account for the up to 500-fold reduction of recombinant formation: (i) preliminary ...

journal_title：Journal of bacteriology

authors： Ou JT,Reim R

更新日期：1976-10-01 00:00:00

abstract：:Somerson, Norman L. (National Institute of Allergy and Infectious Diseases, Bethesda, Md.), and M. K. Cook. Suppression of Rous sarcoma virus growth in tissue cultures by Mycoplasma orale. J. Bacteriol. 90:534-540. 1965.-An agent which produced cell destruction in human diploid and chick-embryo fibroblasts was isolate...

journal_title：Journal of bacteriology

authors： SOMERSON NL,COOK MK

更新日期：1965-08-01 00:00:00

abstract：:The presence of phospho-beta-glucosidases and beta-glucoside permeases was found in strains of Streptococcus, Bacillus, and Staphylococcus. In streptococci, the phospho-beta-glucosidase activity depends on the antigenic group. The highest activity was found in strains of group D. In group D strains, phospho-beta-gluco...

journal_title：Journal of bacteriology

authors： Schaefler S,Malamy A,Green I

更新日期：1969-08-01 00:00:00

abstract：:Polyamines are present in high concentrations in archaea, yet little is known about their synthesis, except by extrapolation from bacterial and eucaryal systems. S-Adenosylmethionine (AdoMet) decarboxylase, a pyruvoyl group-containing enzyme that is required for spermidine biosynthesis, has been previously identified ...

journal_title：Journal of bacteriology

authors： Kim AD,Graham DE,Seeholzer SH,Markham GD

更新日期：2000-12-01 00:00:00

abstract：:Specific localization of the lysis (L) protein of bacteriophage MS2 in the cell wall of Escherichia coli was determined by immunoelectron microscopy. After induction of the cloned lysis gene, the cells were plasmolyzed, fixed, and embedded in either Epon or Lowicryl K4M. Polyclonal L-protein-specific antiserum was pur...

journal_title：Journal of bacteriology

authors： Walderich B,Höltje JV

更新日期：1989-06-01 00:00:00

abstract：UNLABELLED:Pupylation is a posttranslational modification peculiar to actinobacteria wherein proteins are covalently modified with a small protein called the prokaryotic ubiquitin-like protein (Pup). Like ubiquitination in eukaryotes, this phenomenon has been associated with proteasome-mediated protein degradation in m...

journal_title：Journal of bacteriology

authors： Compton CL,Fernandopulle MS,Nagari RT,Sello JK

更新日期：2015-09-01 00:00:00

abstract：:Glycogen accumulation in Escherichia coli is inversely related to the growth rate and occurs most actively when cells enter the stationary phase. The levels of the three biosynthetic enzymes undergo corresponding changes under these conditions, suggesting that genetic control of enzyme biosynthesis may account for at ...

journal_title：Journal of bacteriology

authors： Romeo T,Preiss J

更新日期：1989-05-01 00:00:00

abstract：:The motility of Spirillum volutans is caused by the rotation of each polar flagellar fascicle in a direction opposite to that of the more slowly rotating cell. Both flagella form cones of revolution oriented in the same direction. When the cell reverses its motion, both fascicles simultaneously reverse their rotation ...

journal_title：Journal of bacteriology

authors： Krieg NR,Tomelty JP,Wells JS Jr

更新日期：1967-11-01 00:00:00

abstract：:An investigation of sulfate reduction in B tsnC*7004, a mutant of Escherichia coli lacking thioredoxin, is reported. Although thioredoxin is indispensable for the adenosine 3'-phosphate 5'-phosphosulfate (PAPS) sulfotransferase reaction under the usual conditions of assay in extracts of wild-type cells, the mutant gre...

journal_title：Journal of bacteriology

authors： Tsang ML,Schiff JA

更新日期：1978-04-01 00:00:00

abstract：:Protoplasts of Saccharomyces cerevisiae were mixed with linear DNA plasmids, pGKl1 and pGKl2, isolated from a Kluyveromyces lactis killer strain and treated with polyethylene glycol. Out of 2,000 colonies regenerated on a nonselective medium, two killer transformants were obtained. The pGKl plasmids and the killer cha...

journal_title：Journal of bacteriology

authors： Gunge N,Murata K,Sakaguchi K

更新日期：1982-07-01 00:00:00

abstract：:The human pathogen Vibrio cholerae carries a chromosomal superintegron (SI). The SI contains an array of hundreds of gene cassettes organized in tandem which are stable under conditions when no particular stress is applied to bacteria (such as during laboratory growth). Rearrangements of these cassettes are catalyzed ...

journal_title：Journal of bacteriology

authors： Baharoglu Z,Krin E,Mazel D

更新日期：2012-04-01 00:00:00

abstract：:The transcriptional regulatory protein Rgg coordinates amino acid catabolism and virulence factor expression in Streptococcus pyogenes. We used a proteomic approach to compare cytoplasmic proteins isolated from S. pyogenes wild-type strain NZ131 (serotype M49) to proteins isolated from an rgg mutant strain during the ...

journal_title：Journal of bacteriology

authors： Chaussee MA,Callegari EA,Chaussee MS

更新日期：2004-11-01 00:00:00

abstract：:Genome duplication and segregation normally are completed before cell division in all organisms. The temporal relation of duplication and segregation, however, can vary in bacteria. Chromosomal regions can segregate towards opposite poles as they are replicated or can stay cohered for a considerable period before segr...

journal_title：Journal of bacteriology

authors： Srivastava P,Fekete RA,Chattoraj DK

更新日期：2006-02-01 00:00:00

abstract：:Flagellar biogenesis and hence motility of Vibrio fischeri depends upon the presence of magnesium. In the absence of magnesium, cells contain few or no flagella and are poorly motile or nonmotile. To dissect the mechanism by which this regulation occurs, we screened transposon insertion mutants for those that could mi...

journal_title：Journal of bacteriology

authors： O'Shea TM,Klein AH,Geszvain K,Wolfe AJ,Visick KL

更新日期：2006-12-01 00:00:00

abstract：:Genes involved in the biosynthesis of leucine have been mapped in Bacillus megaterium QM B1551, using transducing phage MP13. Mutations were designated leuA, leuB, or leuC on the basis of enzyme assays. Two mutant strains were deficient in the enzyme activities of leuA (alpha-isopropylmalate synthase) and leuC (beta-i...

journal_title：Journal of bacteriology

authors： Garbe JC,Hess GF,Franzen MA,Vary PS

更新日期：1984-02-01 00:00:00

abstract：:Bacterial spores have long been recognized as the sturdiest known life forms on earth, revealing extraordinary resistance to a broad range of environmental assaults. A family of highly conserved spore-specific DNA-binding proteins, termed alpha/beta-type small, acid-soluble spore proteins (SASP), plays a major role in...

journal_title：Journal of bacteriology

authors： Frenkiel-Krispin D,Sack R,Englander J,Shimoni E,Eisenstein M,Bullitt E,Horowitz-Scherer R,Hayes CS,Setlow P,Minsky A,Wolf SG

更新日期：2004-06-01 00:00:00

abstract：:The inner membrane TET (TetA) protein, which is involved in Tn10-mediated microbial tetracycline resistance, consists of two domains, alpha and beta, both of which are needed for tetracycline resistance and efflux (M.S. Curiale, L.M. McMurry, and S.B. Levy, J. Bacteriol. 157:211-217, 1984). Since tetracycline-sensitiv...

journal_title：Journal of bacteriology

authors： Hickman RK,Levy SB

更新日期：1988-04-01 00:00:00

abstract：:Two riboflavin synthase activities (heavy and light) have been observed in earlier studies with Bacillus subtilis. The heavy enzyme is a complex of one molecule of light enzyme (consisting of three alpha subunits) and approximately 60 beta subunits (A. Bacher, R. Bauer, U. Eggers, H. Harders, and H. Schnepple, p. 729-...

journal_title：Journal of bacteriology

authors： Bacher A,Mailänder B

更新日期：1978-05-01 00:00:00

abstract：:Methicillin resistance in staphylococci is mediated by PBP2a, a penicillin binding protein with low affinity for beta-lactam antibiotics. The gene encoding PBP2a, mecA, is transcriptionally regulated in some clinical isolates by mecR1 and mecI, genes divergently transcribed from mecA that encode a signal transducer an...

journal_title：Journal of bacteriology

authors： Sharma VK,Hackbarth CJ,Dickinson TM,Archer GL

更新日期：1998-04-01 00:00:00

abstract：:Wild-type strains of Escherichia coli K-12 are resistant to acriflavine. Gene acrA(+) which determines resistance to acriflavine is located near the lac region of the chromosome. This gene determines not only resistance to basic dyes but also resistance to phenethyl alcohol. Acriflavine resistance was transmitted, tog...

journal_title：Journal of bacteriology

authors： Nakamura H

更新日期：1968-10-01 00:00:00

abstract：:Genetic circularity of the Pseudomonas aeruginosa PAO chromosome was demonstrated by a series of two- and three-factor crosses and double-selection experiments with Cma plasmids FP2, FP5, FP110, and R68.45. A range of additional markers, including catabolic markers, were located on the chromosome map. Plasmid FP2, kno...

journal_title：Journal of bacteriology

authors： Royle PL,Matsumoto H,Holloway BW

更新日期：1981-01-01 00:00:00

abstract：:The gcaD, prs, and ctc genes were shown to be organized as a tricistronic operon. The transcription of the prs gene, measured as phosphoribosyl diphosphate synthetase activity, and of the ctc gene, measured as beta-galactosidase activity specified by a ctc-lacZ protein fusion, were dependent on the promoter in front o...

journal_title：Journal of bacteriology

authors： Hilden I,Krath BN,Hove-Jensen B

更新日期：1995-12-01 00:00:00

abstract：:We constructed a conditional mutant of pbpA in which transcription of the gene was placed under the control of an IPTG (isopropyl-beta-D-thiogalactopyranoside)-inducible promoter in order to explore the role of PBP1 in growth, cell wall structure, and cell division. A methicillin-resistant strain and an isogenic methi...

journal_title：Journal of bacteriology

authors： Pereira SF,Henriques AO,Pinho MG,de Lencastre H,Tomasz A

更新日期：2007-05-01 00:00:00

abstract：:The CbpA protein is an analog of the DnaJ molecular chaperone of Escherichia coli. To gain insight into the function of CbpA, we examined the nature of a cbpA null mutation with special reference to those of dnaK and dnaJ null mutations. In particular, the cbpA dnaJ double-null mutant was found to exhibit severe defec...

journal_title：Journal of bacteriology

authors： Ueguchi C,Shiozawa T,Kakeda M,Yamada H,Mizuno T

更新日期：1995-07-01 00:00:00

abstract：:In the nitrogenase system of Klebsiella pneumoniae, nifT is located between nifDK, the structural genes for dinitrogenase, and nifY, whose product is involved in nitrogenase maturation. It is, therefore, a reasonable hypothesis that the NifT protein might also have a role in the maturation of nitrogenase. However, the...

journal_title：Journal of bacteriology

authors： Simon HM,Homer MJ,Roberts GP

更新日期：1996-05-01 00:00:00

abstract：:The light organ of the squid Euprymna scolopes is specifically colonized to a high density by the marine bacterium Vibrio fischeri. To date, only a few factors contributing to the specificity of this symbiosis have been identified. Using a genetic screen for random transposon mutants defective in initiating the symbio...

journal_title：Journal of bacteriology

authors： Visick KL,Skoufos LM

更新日期：2001-02-01 00:00:00

abstract：:spo0A and spo0H are needed for the initiation of sporulation and for the development of genetic competence in Bacillus subtilis. Transcription of spo0A initiates from two promoters, Pv and Ps. Pv is active during vegetative growth and is recognized by RNA polymerase containing sigma A. Expression from Ps increases dur...

journal_title：Journal of bacteriology

authors： Siranosian KJ,Grossman AD

更新日期：1994-06-01 00:00:00

abstract：UNLABELLED:Bacterial type IV coupling proteins (T4CPs) bind and mediate the delivery of DNA substrates through associated type IV secretion systems (T4SSs). T4CPs consist of a transmembrane domain, a conserved nucleotide-binding domain (NBD), and a sequence-variable helical bundle called the all-alpha domain (AAD). In ...

journal_title：Journal of bacteriology

authors： Whitaker N,Chen Y,Jakubowski SJ,Sarkar MK,Li F,Christie PJ

更新日期：2015-07-01 00:00:00

abstract：:Procedures were developed to facilitate the identification of genes that belong to a given regulon and characterization of their responses to the regulator. The regulon controlled by the Escherichia coli leucine-responsive regulatory protein (Lrp) was studied by isolating random transcriptional fusions to lacZ, using ...

journal_title：Journal of bacteriology

authors： Bhagwat SP,Rice MR,Matthews RG,Blumenthal RM

更新日期：1997-10-01 00:00:00