Tricistronic operon expression of the genes gcaD (tms), which encodes N-acetylglucosamine 1-phosphate uridyltransferase, prs, which encodes phosphoribosyl diphosphate synthetase, and ctc in vegetative cells of Bacillus subtilis.

Abstract:

:The gcaD, prs, and ctc genes were shown to be organized as a tricistronic operon. The transcription of the prs gene, measured as phosphoribosyl diphosphate synthetase activity, and of the ctc gene, measured as beta-galactosidase activity specified by a ctc-lacZ protein fusion, were dependent on the promoter in front of the gcaD gene. Analysis of cDNA molecules prepared with gcaD-prs-ctc-specified mRNA as the template revealed an RNA transcript that encompassed all three cistrons.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Hilden I,Krath BN,Hove-Jensen B

doi

10.1128/jb.177.24.7280-7284.1995

subject

Has Abstract

pub_date

1995-12-01 00:00:00

pages

7280-4

issue

24

eissn

0021-9193

issn

1098-5530

journal_volume

177

pub_type

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