Transformation of Saccharomyces cerevisiae with linear DNA killer plasmids from Kluyveromyces lactis.

abstract：:Burkholderia thailandensis is a soil saprophyte that is closely related to the pathogen Burkholderia pseudomallei, the etiological agent of melioidosis in humans. The environmental niches and infection sites occupied by these bacteria are thought to contain only limited concentrations of oxygen, where they can generat...

journal_title：Journal of bacteriology

authors： Vitale A,Paszti S,Takahashi K,Toyofuku M,Pessi G,Eberl L

更新日期：2020-11-04 00:00:00

abstract：:Broad-host-range plasmids and cloning vectors were conjugatively transferred to Acetobacter xylinum. One of the plasmids, RP4::Mu cts61, was used for the insertion of Tn1 into the 16-, 44-, and 64-kilobase-pair plasmids of A. xylinum. The Tn1-labeled plasmids could be mobilized by a helper plasmid. Many of the Tn1 ins...

journal_title：Journal of bacteriology

authors： Valla S,Coucheron DH,Kjosbakken J

更新日期：1986-01-01 00:00:00

abstract：:Mutations in the tcmII-tcmIV region of the Streptomyces glaucescens chromosome block the C-3 and C-8 O-methylations of the polyketide antibiotic tetracenomycin C (Tcm C). The nucleotide sequence of this region reveals the presence of two genes, tcmN and tcmO, whose deduced protein products display similarity to the hy...

journal_title：Journal of bacteriology

authors： Summers RG,Wendt-Pienkowski E,Motamedi H,Hutchinson CR

更新日期：1992-03-01 00:00:00

abstract：:Wall turnover was studied in Bacillus subtilis. The loss of radioactively labeled wall polymers was followed during exponential growth in batch and chemostat cultures. Turnover kinetics were identical under all growth conditions; pulse-labeled wall material was lost with first-order kinetics, but only after exponentia...

journal_title：Journal of bacteriology

authors： De Boer WR,Kruyssen FJ,Wouters JT

更新日期：1981-01-01 00:00:00

abstract：:Sagers, Richard D. (Brigham Young University, Provo, Utah), Moshe Benziman, and Sigrid M. Klein. Failure of arsenate to uncouple the phosphotransacetylase system in Clostridium acidiurici. J. Bacteriol. 86:978-984. 1963.-The conversion of pyruvate to acetyl phosphate by extracts of Clostridium acidiurici required coen...

journal_title：Journal of bacteriology

authors： SAGERS RD,BENZIMAN M,KLEIN SM

更新日期：1963-11-01 00:00:00

abstract：:31P and 13C nuclear magnetic resonance (NMR) experiments were performed on suspensions of the phototrophic bacterium Chromatium vinosum incubated anaerobically in the dark. 31P NMR spectra revealed that during prolonged dark incubation high ATP levels are maintained. This phenomenon was independent of the presence of ...

journal_title：Journal of bacteriology

authors： Nicolay K,van Gemerden H,Hellingwerf KJ,Konings WN,Kaptein R

更新日期：1983-08-01 00:00:00

abstract：:lac operon fusions to DNA damage-inducible (din) loci were generated in Salmonella typhimurium LT2. Many of these din fusions were efficiently repressed by cloned Escherichia coli LexA, while others were not; all required RecA for induction. Several din fusions exhibited strong inducibility and will be useful in devel...

journal_title：Journal of bacteriology

authors： Smith CM,Arany Z,Orrego C,Eisenstadt E

更新日期：1991-06-01 00:00:00

abstract：:Methyl coenzyme M methylreductase from acetate-grown Methanosarcina thermophila TM-1 was purified 16-fold from a cell extract to apparent homogeneity as determined by native polyacrylamide gel electrophoresis. Ninety-four percent of the methylreductase activity was recovered in the soluble fraction of cell extracts. T...

journal_title：Journal of bacteriology

authors： Jablonski PE,Ferry JG

更新日期：1991-04-01 00:00:00

abstract：:Isolated membranes of Bacillus stearothermophilus 2184D can be disrupted by treatment with sodium dodecyl sulfate (SDS). This disruption is attended by a decreased turbidity of membrane suspensions and a differential loss of activities of the electron transport system. Reduced methyl viologen (MVH)-nitrate reductase a...

journal_title：Journal of bacteriology

authors： Kiszkiss DF,Downey RJ

更新日期：1972-02-01 00:00:00

abstract：:The lethal activity of a thymineless mutant of Staphylococcus aureus Wood 46 strain has been compared with that of three naturally occurring strains: parent Wood 46, Smith, and coagulase-negative SA-13. The thymineless mutant and the parent Wood 46 strain showed a sharp decline in culturable units from the peritoneal ...

journal_title：Journal of bacteriology

authors： Mathieu LG,De Repentigny J,Turgeon S,Sonea S

更新日期：1967-07-01 00:00:00

abstract：:A late-log-phase culture of an Escherichia coli nadB pncA double mutant took up 6-[7-14C]aminonicotinic acid and excreted 6-[14C]aminonicotinamide. This mutant also accumulated intracellularly several radioactive compounds which have been tentatively identified as 6-amino analogs of compounds in the pyridine nucleotid...

journal_title：Journal of bacteriology

authors： Cobb JR,Pearcy SC,Gholson RK

更新日期：1977-09-01 00:00:00

abstract：:The temperature-sensitive cyr1-2 mutant in Saccharomyces cerevisiae produces low levels of adenylate cyclase and cyclic AMP at 25 degrees C and is unable to synthesize repressible acid phosphatase at 25 degrees C. Suppressor mutants of cyr1-2 were isolated by detecting acid phosphatase activity. One of the dominant su...

journal_title：Journal of bacteriology

authors： Morishita T,Uno I

更新日期：1991-04-01 00:00:00

abstract：:Ganesan, Ann K. (Syntex Institute of Molecular Biology, Palo Alto, Calif.), and Boris Rotman. Transfer and incorporation of genes controlling beta-d-galactosidase synthesis from Hfr and F' donors of Escherichia coli. J. Bacteriol. 92:1378-1382. 1966.-Comparisons were made between Hfr(1) and F(13) donors with respect t...

journal_title：Journal of bacteriology

authors： Ganesan AK,Rotman B

更新日期：1966-11-01 00:00:00

abstract：:MalK is a peripheral cytoplasmic membrane protein that has multiple activities in Escherichia coli. It associates with integral cytoplasmic membrane proteins MalF and MalG to form the maltose transport complex (MalFGK), a member of the ATP-binding cassette (ABC) superfamily of proteins. In addition, MalK participates ...

journal_title：Journal of bacteriology

authors： Lippincott J,Traxler B

更新日期：1997-02-01 00:00:00

abstract：:Streptomyces davawensis JCM 4913 synthesizes the antibiotic roseoflavin, a structural riboflavin (vitamin B(2)) analog. Here, we report the 9,466,619-bp linear chromosome of S. davawensis JCM 4913 and a 89,331-bp linear plasmid. The sequence has an average G+C content of 70.58% and contains six rRNA operons (16S-23S-5...

journal_title：Journal of bacteriology

authors： Jankowitsch F,Schwarz J,Rückert C,Gust B,Szczepanowski R,Blom J,Pelzer S,Kalinowski J,Mack M

更新日期：2012-12-01 00:00:00

abstract：:To isolate genes from Escherichia coli which regulate the labile hydrogenase activity, a plasmid library was used to transform hydL mutants lacking the labile hydrogenase. A single type of gene, designated hydG, was isolated. This gene also partially restored the hydrogenase activity in hydF mutants (which are defecti...

journal_title：Journal of bacteriology

authors： Stoker K,Reijnders WN,Oltmann LF,Stouthamer AH

更新日期：1989-08-01 00:00:00

abstract：:Legionella pneumophila expresses two peroxide-scavenging alkyl hydroperoxide reductase systems (AhpC1 and AhpC2D) that are expressed differentially during the bacterial growth cycle. Functional loss of the postexponentially expressed AhpC1 system is compensated for by increased expression of the exponentially expresse...

journal_title：Journal of bacteriology

authors： LeBlanc JJ,Brassinga AK,Ewann F,Davidson RJ,Hoffman PS

更新日期：2008-05-01 00:00:00

abstract：:Weiss, K. F. (Iowa State University, Ames), J. C. Ayres, and A. A. Kraft. Inhibitory action of selenite on Escherichia coli, Proteus vulgaris, and Salmonella thompson. J. Bacteriol. 90:857-862. 1965.-The resistance of three microorganisms, Escherichia coli (ISU-41), Proteus vulgaris (ISU-37c), and Salmonella thompson ...

journal_title：Journal of bacteriology

authors： Weiss KF,Ayres JC,Kraft AA

更新日期：1965-10-01 00:00:00

abstract：:Numerous field isolates of Moraxella bovis have previously been classified by serological techniques into seven serogroups, each defined by homologous cross-reaction with antisera prepared against purified pili of a single prototype strain. The gene encoding pilin from each of the prototype strains has been characteri...

journal_title：Journal of bacteriology

authors： Atwell JL,Tennent JM,Lepper AW,Elleman TC

更新日期：1994-08-01 00:00:00

abstract：:In Pseudomonas aeruginosa, flagellar genes are regulated in a cascade headed by FleQ, an NtrC/NifA-type activator. FleQ and RpoN positively regulate expression of flhA, fliE, fliL, and fleSR genes, among others. Direct interaction of FleQ with flhA, fliE, fliL, and fleSR promoters was demonstrated by gel shift assay, ...

journal_title：Journal of bacteriology

authors： Jyot J,Dasgupta N,Ramphal R

更新日期：2002-10-01 00:00:00

abstract：:The parDE operon, located within the 3.2-kb stabilization region of plasmid RK2, encodes antitoxin (ParD) and toxin (ParE) proteins that stabilize the maintenance of this broad-host-range plasmid via a postsegregational killing mechanism. A ParE protein derivative, designated ParE', was purified by construction of a f...

journal_title：Journal of bacteriology

authors： Johnson EP,Strom AR,Helinski DR

更新日期：1996-03-01 00:00:00

abstract：:Two sets of genes for the large and small subunits of ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO) were detected in the photosynthetic purple sulfur bacterium Chromatium vinosum by hybridization analysis with RuBisCO gene probes, cloned by using the lambda Fix vector, and designated rbcL-rbcS and rbcA-rbc...

journal_title：Journal of bacteriology

authors： Viale AM,Kobayashi H,Akazawa T

更新日期：1989-05-01 00:00:00

abstract：:Purified L-asparaginase from Serratia marcescens had an apparent-weight average molecular weight of 171,000 to 180,000 as determined by electrophoresis on polyacrylamide gels and by sedimentation equilibrium at low speed in an analytical ultracentrifuge. A subunit molecular weight of 31,500 +/- 1,500 was estimated for...

journal_title：Journal of bacteriology

authors： Stern ML,Phillips AW,Gottlieb AJ

更新日期：1976-02-01 00:00:00

abstract：:DnaA is an AAA+ ATPase and the conserved replication initiator in bacteria. Bacteria control the timing of replication initiation by regulating the activity of DnaA. DnaA binds to multiple sites in the origin of replication (oriC) and is required for recruitment of proteins needed to load the replicative helicase. Dna...

journal_title：Journal of bacteriology

authors： Bonilla CY,Grossman AD

更新日期：2012-09-01 00:00:00

abstract：:Loss of the ability of Pseudomonas syringae pv. "phaseolicola" NPS3121 to elicit a hypersensitive response on tobacco and other nonhost plants was associated with loss of pathogenicity on the susceptible host bean. Eight independent, prototrophic transposon Tn5 insertion mutants which had lost the ability to elicit a ...

journal_title：Journal of bacteriology

authors： Lindgren PB,Peet RC,Panopoulos NJ

更新日期：1986-11-01 00:00:00

abstract：:Strains of Escherichia coli K12 have been prepared that carry the ruv mutation in combination with lon or uvrA mutant alleles. The ruv minus uvrA minus double mutant is more sensitive to ultraviolet light than the urvA minus single mutant, whereas the strain with ruv and ion mutations shows the same sensitivity to ult...

journal_title：Journal of bacteriology

authors： Iyehara H,Otsuji N

更新日期：1975-02-01 00:00:00

abstract：:Entry into the host bacterial cell is one of the least understood steps in the life cycle of bacteriophages. The different envelopes of Gram-negative and Gram-positive bacteria, with a fluid outer membrane and exposing a thick peptidoglycan wall to the environment respectively, impose distinct challenges for bacteriop...

journal_title：Journal of bacteriology

authors： Jakutytė L,Baptista C,São-José C,Daugelavičius R,Carballido-López R,Tavares P

更新日期：2011-09-01 00:00:00

abstract：:The reproduction and morphogenesis of Rickettsiella chironomi, an unusual procaryotic parasite of midge larvae, was studied with electron microscopy. The morphogenic cycle was similar to that found in chlamydia and consisted of four major cell types: (i) medium-sized spherical initial bodies (ca. 1 micrometers in diam...

journal_title：Journal of bacteriology

authors： Federici BA

更新日期：1980-08-01 00:00:00

abstract：:The transglycosylation step of cell wall synthesis is a prime antibiotic target because it is essential and specific to bacteria. Two antibiotics, ramoplanin and moenomycin, target this step by binding to the substrate lipid II and the transglycosylase enzyme, respectively. Here, we compare the ramoplanin and moenomyc...

journal_title：Journal of bacteriology

authors： Salzberg LI,Luo Y,Hachmann AB,Mascher T,Helmann JD

更新日期：2011-10-01 00:00:00

abstract：:The transferred DNA (T-DNA) is transported from Agrobacterium tumefaciens to the nucleus and is stably integrated into the genome of many plant species. It has been proposed that the VirD2 protein, tightly attached to the T-DNA, pilots the T-DNA into the plant cell nucleus and that it is involved in integration. Using...

journal_title：Journal of bacteriology

authors： Koukolíková-Nicola Z,Raineri D,Stephens K,Ramos C,Tinland B,Nester EW,Hohn B

更新日期：1993-02-01 00:00:00