Structure of the DNA-SspC complex: implications for DNA packaging, protection, and repair in bacterial spores.

Abstract:

:Bacterial spores have long been recognized as the sturdiest known life forms on earth, revealing extraordinary resistance to a broad range of environmental assaults. A family of highly conserved spore-specific DNA-binding proteins, termed alpha/beta-type small, acid-soluble spore proteins (SASP), plays a major role in mediating spore resistance. The mechanism by which these proteins exert their protective activity remains poorly understood, in part due to the lack of structural data on the DNA-SASP complex. By using cryoelectron microscopy, we have determined the structure of the helical complex formed between DNA and SspC, a characteristic member of the alpha/beta-type SASP family. The protein is found to fully coat the DNA, forming distinct protruding domains, and to modify DNA structure such that it adopts a 3.2-nm pitch. The protruding SspC motifs allow for interdigitation of adjacent DNA-SspC filaments into a tightly packed assembly of nucleoprotein helices. By effectively sequestering DNA molecules, this dense assembly of filaments is proposed to enhance and complement DNA protection obtained by DNA saturation with the alpha/beta-type SASP.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Frenkiel-Krispin D,Sack R,Englander J,Shimoni E,Eisenstein M,Bullitt E,Horowitz-Scherer R,Hayes CS,Setlow P,Minsky A,Wolf SG

doi

10.1128/JB.186.11.3525-3530.2004

keywords:

subject

Has Abstract

pub_date

2004-06-01 00:00:00

pages

3525-30

issue

11

eissn

0021-9193

issn

1098-5530

pii

186/11/3525

journal_volume

186

pub_type

杂志文章
  • Two nitrate/nitrite transporters are encoded within the mobilizable plasmid for nitrate respiration of Thermus thermophilus HB8.

    abstract::Thermus thermophilus HB8 can grow anaerobically by using a membrane-bound nitrate reductase to catalyze the reduction of nitrate as a final electron acceptor in respiration. In contrast to other denitrifiers, the nitrite produced does not continue the reduction pathway but accumulates in the growth medium after its ac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.8.2179-2183.2000

    authors: Ramírez S,Moreno R,Zafra O,Castán P,Vallés C,Berenguer J

    更新日期:2000-04-01 00:00:00

  • Fur regulates expression of the Salmonella pathogenicity island 1 type III secretion system through HilD.

    abstract::The invasion of intestinal epithelial cells by Salmonella enterica serovar Typhimurium is mediated by a type III secretion system (T3SS) encoded on Salmonella pathogenicity island 1 (SPI1). Expression of the SPI1 T3SS is tightly regulated by the combined action of HilC, HilD, and RtsA, three AraC family members that c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00926-07

    authors: Ellermeier JR,Slauch JM

    更新日期:2008-01-01 00:00:00

  • Cosmid cloning of five Zymomonas trp genes by complementation of Escherichia coli and Pseudomonas putida trp mutants.

    abstract::A library of Zymomonas mobilis genomic DNA was constructed in the broad-host-range cosmid pLAFR1. The library was mobilized into a variety of Escherichia coli and Pseudomonas putida trp mutants by using the helper plasmid pRK2013. Five Z. mobilis trp genes were identified by the ability to complement the trp mutants. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.7.3158-3163.1988

    authors: Eddy CK,Smith OH,Noel KD

    更新日期:1988-07-01 00:00:00

  • Chemical modification of Streptococcus flagellar motors.

    abstract::Video techniques were used to record changes in motility of cells of Streptococcus sp. strain V4051 exposed to a variety of protein modification reagents. Starved cells were tethered to glass by a single flagellum, energized metabolically with glucose, or treated with valinomycin and energized artificially via shifts ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.158.3.832-843.1984

    authors: Conley MP,Berg HC

    更新日期:1984-06-01 00:00:00

  • The ompB operon partially determines differential expression of OmpC in Salmonella typhi and Escherichia coli.

    abstract::Expression of the Escherichia coli OmpC and OmpF outer membrane proteins is regulated by the osmolarity of the culture media. In contrast, expression of OmpC in Salmonella typhi is not influenced by osmolarity, while OmpF is regulated as in E. coli. To better understand the lack of osmoregulation of OmpC expression in...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.2.556-562.1999

    authors: Martínez-Flores I,Cano R,Bustamante VH,Calva E,Puente JL

    更新日期:1999-01-01 00:00:00

  • CHANGES IN MOUSE PNEUMONITIS AGENT ASSOCIATED WITH DEVELOPMENT OF RESISTANCE TO CHLORTETRACYCLINE.

    abstract::Moulder, James W. (University of Chicago, Chicago, Ill.), Dorothy L. Novosel, and Ilse I. E. Tribby. Changes in mouse pneumonitis agent associated with development of resistance to chlortetracycline. J. Bacteriol. 89:17-22. 1965.-A chlortetracycline-resistant mutant of mouse pneumonitis agent, a member of the psittaco...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.1.17-22.1965

    authors: MOULDER JW,NOVOSEL DL,TRIBBY II

    更新日期:1965-01-01 00:00:00

  • Influence of methionine pool composition on the formation of methyl-deficient transfer ribonucleic acid in Saccharomyces cerevisiae.

    abstract::Methionine auxotrophs of Saccharomyces cerevisiae continue to synthesize ribonucleic acid (RNA) after methionine withdrawal. The newly synthesized transfer RNA (tRNA) is methyl-deficient in some strains, but not in all. Whether such tRNA will accumulate depends on the position of the block in the methionine pathway th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.2.687-694.1969

    authors: Kjellin-Stråby K

    更新日期:1969-11-01 00:00:00

  • Comparison of lipid composition of pleuropneumonia-like and L-type organisms.

    abstract::Smith, Paul F. (University of Pennsylvania, Philadelphia) and George H. Rothblat. Comparison of pleuropneumonia-like and L-type organisms. J. Bacteriol. 83:500-506. 1962.-The content of total lipid and nonsaponifiable lipid depended upon the type of organism; the greatest amounts were found in pleuropneumonia-like org...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.3.500-506.1962

    authors: SMITH PF,ROTHBLAT GH

    更新日期:1962-03-01 00:00:00

  • Evidence for distinct kynureninase and hydroxykynureninase activities in Neurospora crassa.

    abstract::Previous studies have indicated that a single enzyme, "kynureninase," catalyzes the reactions of l-kynurenine to anthranilate and l-3-hydroxykynurenine to 3-hydroxyanthranilate in Neurospora crassa and in other organisms. The present report describes separate enzymes which catalyze these reactions in N. crassa. The fi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.108.2.902-909.1971

    authors: Gaertner FH,Cole KW,Welch GR

    更新日期:1971-11-01 00:00:00

  • Catabolite repression and induction of the Mg(2+)-citrate transporter CitM of Bacillus subtilis.

    abstract::In Bacillus subtilis the citM gene encodes the Mg(2+)-citrate transporter. A target site for carbon catabolite repression (cre site) is located upstream of citM. Fusions of the citM promoter region, including the cre sequence, to the beta-galactosidase reporter gene were constructed and integrated into the amyE site o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.21.6099-6105.2000

    authors: Warner JB,Krom BP,Magni C,Konings WN,Lolkema JS

    更新日期:2000-11-01 00:00:00

  • Unique regulatory mechanism of sporulation and enterotoxin production in Clostridium perfringens.

    abstract::Clostridium perfringens causes gas gangrene and gastrointestinal (GI) diseases in humans. The most common cause of C. perfringens-associated food poisoning is the consumption of C. perfringens vegetative cells followed by sporulation and production of enterotoxin in the gut. Despite the importance of spore formation i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02152-12

    authors: Ohtani K,Hirakawa H,Paredes-Sabja D,Tashiro K,Kuhara S,Sarker MR,Shimizu T

    更新日期:2013-06-01 00:00:00

  • Flk couples flgM translation to flagellar ring assembly in Salmonella typhimurium.

    abstract::The hook-basal body (HBB) is a key intermediate structure in the flagellar assembly pathway in Salmonella typhimurium. The FlgM protein inhibits the flagellum-specific transcription factor sigma28 in the absence of the intact HBB structure and is secreted out of the cell following HBB completion. The flk gene encodes ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.20.5384-5397.1998

    authors: Karlinsey JE,Tsui HC,Winkler ME,Hughes KT

    更新日期:1998-10-01 00:00:00

  • All seven comG open reading frames are required for DNA binding during transformation of competent Bacillus subtilis.

    abstract::The seven proteins encoded by the comG operon of Bacillus subtilis exhibit similarity to gene products required for the assembly of type 4 pili and for the secretion of certain proteins in gram-negative bacteria. Although polar transposon insertions in comG result in the loss of transformability and in the failure of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.1.41-45.1998

    authors: Chung YS,Dubnau D

    更新日期:1998-01-01 00:00:00

  • A mutation in the Neisseria gonorrhoeae rfaD homolog results in altered lipooligosaccharide expression.

    abstract::The gonococcal lsi-6 locus was cloned and shown by DNA sequence analysis to have homology with the E. coli rfaD gene, which encodes ADP-L-glycero-D-mannoheptose epimerase. This enzyme is involved in the biosynthesis of the lipopolysaccharide precursor ADP-L-glycero-D-mannoheptose. A site-directed frameshift mutation i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.9.2321-2327.1995

    authors: Drazek ES,Stein DC,Deal CD

    更新日期:1995-05-01 00:00:00

  • Identification of an Escherichia coli operon required for formation of the O-antigen capsule.

    abstract::Escherichia coli produces polysaccharide capsules that, based on their mechanisms of synthesis and assembly, have been classified into four groups. The group 4 capsule (G4C) polysaccharide is frequently identical to that of the cognate lipopolysaccharide O side chain and has, therefore, also been termed the O-antigen ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.15.5259-5266.2005

    authors: Peleg A,Shifrin Y,Ilan O,Nadler-Yona C,Nov S,Koby S,Baruch K,Altuvia S,Elgrably-Weiss M,Abe CM,Knutton S,Saper MA,Rosenshine I

    更新日期:2005-08-01 00:00:00

  • Competitive inhibition of transformation in group H Streptococcus strain Challis by heterologous deoxyribonucleic acid.

    abstract::Glucosylated deoxyribonucleic acid (DNA) from phages T4 and T6 competes poorly with homologous DNA causing only a slight decrease of transformation in Group H Streptococcus strain Challis. Other types of heterologous DNAs (Micrococcus luteus, Clostridium perfringens, Escherichia coli, calf thymus and non-glucosylated ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.124.3.1621-1623.1975

    authors: Ceglowski P,Fuchs PG,Soltyk A

    更新日期:1975-12-01 00:00:00

  • Magnesium Suppresses Defects in the Formation of 70S Ribosomes as Well as in Sporulation Caused by Lack of Several Individual Ribosomal Proteins.

    abstract::Individually, the ribosomal proteins L1, L23, L36, and S6 are not essential for cell proliferation of Bacillus subtilis, but the absence of any one of these ribosomal proteins causes a defect in the formation of the 70S ribosomes and a reduced growth rate. In mutant strains individually lacking these ribosomal protein...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00212-18

    authors: Akanuma G,Yamazaki K,Yagishi Y,Iizuka Y,Ishizuka M,Kawamura F,Kato-Yamada Y

    更新日期:2018-08-24 00:00:00

  • Proteins induced in Escherichia coli by benzoic acid.

    abstract::Proteins induced by benzoic acid in Escherichia coli were observed on two-dimensional electrophoretic gels (2-D gels). Cultures were grown in glucose-rich medium in the presence or absence of 20 mM benzoate at an external pH of 6.5, where the pH gradient (deltapH) is large and benzoate accumulates, and at an external ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.23.7595-7599.1997

    authors: Lambert LA,Abshire K,Blankenhorn D,Slonczewski JL

    更新日期:1997-12-01 00:00:00

  • Effect of growth conditions on production of rhamnose-containing cell wall and capsular polysaccharides by strains of Lactobacillus casei subsp. rhamnosus.

    abstract::Strains of Lactobacillus casei subsp. rhamnosus possessing two cell wall polysaccharides, a hexosamine-containing H-polysaccharide and a rhamnose-containing R-polysaccharide, were examined for the effect of growth conditions on the production of these two components. In strain NCTC 6375, R- and H-polysaccharides accou...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.153.1.84-92.1983

    authors: Wicken AJ,Ayres A,Campbell LK,Knox KW

    更新日期:1983-01-01 00:00:00

  • The Xanthomonas oryzae pv. oryzae PhoPQ two-component system is required for AvrXA21 activity, hrpG expression, and virulence.

    abstract::The rice pathogen recognition receptor, XA21, confers resistance to Xanthomonas oryzae pv. oryzae strains producing the type one system-secreted molecule, AvrXA21. X. oryzae pv. oryzae requires a regulatory two-component system (TCS) called RaxRH to regulate expression of eight rax (required for AvrXA21 activity) gene...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01406-07

    authors: Lee SW,Jeong KS,Han SW,Lee SE,Phee BK,Hahn TR,Ronald P

    更新日期:2008-03-01 00:00:00

  • Phylogeny of dissimilatory sulfite reductases supports an early origin of sulfate respiration.

    abstract::Microorganisms that use sulfate as a terminal electron acceptor for anaerobic respiration play a central role in the global sulfur cycle. Here, we report the results of comparative sequence analysis of dissimilatory sulfite reductase (DSR) genes from closely and distantly related sulfate-reducing organisms to infer th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.11.2975-2982.1998

    authors: Wagner M,Roger AJ,Flax JL,Brusseau GA,Stahl DA

    更新日期:1998-06-01 00:00:00

  • Programmed translational frameshift in the bacteriophage P2 FETUD tail gene operon.

    abstract::The major structural components of the P2 contractile tail are encoded in the FETUD tail gene operon. The sequences of genes F(I) and F(II), encoding the major tail sheath and tail tube proteins, have been reported previously (L. M. Temple, S. L. Forsburg, R. Calendar, and G. E. Christie, Virology 181:353-358, 1991). ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.23.6522-6531.2002

    authors: Christie GE,Temple LM,Bartlett BA,Goodwin TS

    更新日期:2002-12-01 00:00:00

  • LuxArray, a high-density, genomewide transcription analysis of Escherichia coli using bioluminescent reporter strains.

    abstract::A sequenced collection of plasmid-borne random fusions of Escherichia coli DNA to a Photorhabdus luminescens luxCDABE reporter was used as a starting point to select a set of 689 nonredundant functional gene fusions. This group, called LuxArray 1.0, represented 27% of the predicted transcriptional units in E. coli. Hi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.19.5496-5505.2001

    authors: Van Dyk TK,DeRose EJ,Gonye GE

    更新日期:2001-10-01 00:00:00

  • Bacteriophage HP2 of Haemophilus influenzae.

    abstract::Temperate bacteriophages effect chromosomal evolution of their bacterial hosts, mediating rearrangements and the acquisition of novel genes from other taxa. Although the Haemophilus influenzae genome shows evidence of past phage-mediated lateral transfer, the phages presumed responsible have not been identified. To da...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.24.6893-6905.2002

    authors: Williams BJ,Golomb M,Phillips T,Brownlee J,Olson MV,Smith AL

    更新日期:2002-12-01 00:00:00

  • Separation of chromosome termini during sporulation of Bacillus subtilis depends on SpoIIIE.

    abstract::Bacillus subtilis undergoes a highly distinctive division during spore formation. It yields two unequal cells, the mother cell and the prespore, and septum formation is completed before the origin-distal 70% of the chromosome has entered the smaller prespore. The mother cell subsequently engulfs the prespore. Two diff...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01949-06

    authors: Bogush M,Xenopoulos P,Piggot PJ

    更新日期:2007-05-01 00:00:00

  • Ethylmalonyl coenzyme A mutase operates as a metabolic control point in Methylobacterium extorquens AM1.

    abstract::The metabolism of one- and two-carbon compounds by the methylotrophic bacterium Methylobacterium extorquens AM1 involves high carbon flux through the ethylmalonyl coenzyme A (ethylmalonyl-CoA) pathway (EMC pathway). During growth on ethylamine, the EMC pathway operates as a linear pathway carrying the full assimilator...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02478-14

    authors: Good NM,Martinez-Gomez NC,Beck DA,Lidstrom ME

    更新日期:2015-02-15 00:00:00

  • Pseudomonas cepacia mutants blocked in the Entner-Doudoroff pathway.

    abstract::Pseudomonas cepacia mutants deficient in either 6-phosphogluconate (6PGA) dehydratase (Edd-) or 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase (Eda-) failed to utilize glucose or gluconate despite the prominence of of 6-phosphogluconate dehydrogenase (6PGAD) ii this bacterium and the potential for utilizing the pen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.150.3.1340-1347.1982

    authors: Allenza P,Lessie TG

    更新日期:1982-06-01 00:00:00

  • Peptidoglycan synthesis in cocci and rods of a pH-dependent, morphologically conditional mutant of Klebsiella pneumoniae.

    abstract::Mir M7 is a spontaneous morphologically conditional mutant of Klebsiella pneumoniae which grows as round cells (cocci) at pH 7 and as normal rods at pH 5.8. We studied the rates of peptidoglycan synthesis of cocci and rods growing at pH values of 7 and 5.8, respectively. It was found that exponentially growing cocci p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.137.2.727-734.1979

    authors: Satta G,Fontana R,Canepari P,Botta G

    更新日期:1979-02-01 00:00:00

  • Characterization and use of catabolite-repressed promoters from gluconate genes in Corynebacterium glutamicum.

    abstract::The genes involved in gluconate catabolism (gntP and gntK) in Corynebacterium glutamicum are scattered in the chromosome, and no regulatory genes are apparently associated with them, in contrast with the organization of the gnt operon in Escherichia coli and Bacillus subtilis. In C. glutamicum, gntP and gntK are essen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.2.409-423.2006

    authors: Letek M,Valbuena N,Ramos A,Ordóñez E,Gil JA,Mateos LM

    更新日期:2006-01-01 00:00:00

  • Isolation of a metabolite capable of differentially supporting the growth of nicotinamide adenine dinucleotide auxotrophs of Escherichia coli.

    abstract::A compound, isolated from the culture fluid of a nadC auxotroph of Escherichia coli grown in a minimal medium, supports the growth of both a nadA and a nadB mutant. This metabolite exhibits an ultraviolet light absorption spectrum and a mass spectrum, different from quinolinic acid. This compound may be the precursor ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.121.1.212-218.1975

    authors: Chen JL,Tritz GJ

    更新日期:1975-01-01 00:00:00