Protoplast formation and leakage of intramembrane cell components: induction by the competence activator substance of pneumococci.

Abstract:

:Treatment of pneumococci with activator (a protein that induces bacterial "competence" to absorb deoxyribonucleic acid molecules and undergo genetic transformation) can cause either protoplast formation or leakage of intracellular components to the medium depending on postincubation conditions. The leaked intracellular components include nucleoside phosphates, beta-galactosidase, deoxyribonuclease, autolysin, and hemolysin. Leakage and protoplast formation are induced by the electrophoretically pure activator, and these phenomena require the same conditions as induction of competence for genetic transformation, namely, genetic capacity for competence, protein synthesis, incorporation of choline, and the optimal pH for activation. It is suggested that the activator protein accelerates a normal process of transport (leakage) of autolysin molecules into the periplasmic space. The activity of these autolysin molecules from within would then unmask deoxyribonucleic acid binding sites located on the plasma membrane.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Seto H,Tomasz A

doi

10.1128/JB.121.1.344-353.1975

subject

Has Abstract

pub_date

1975-01-01 00:00:00

pages

344-53

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

121

pub_type

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