Lactococcin A, a new bacteriocin from Lactococcus lactis subsp. cremoris: isolation and characterization of the protein and its gene.

Abstract:

:A new bacteriocin, termed lactococcin A (LCN-A), from Lactococcus lactis subsp. cremoris LMG 2130 was purified and sequenced. The polypeptide contained no unusual amino acids and showed no significant sequence similarity to other known proteins. Only lactococci were killed by the bacteriocin. Of more than 120 L. lactis strains tested, only 1 was found resistant to LCN-A. The most sensitive strain tested, L. lactis subsp. cremoris NCDO 1198, was inhibited by 7 pM LCN-A. By use of a synthetic DNA probe, lcnA was found to be located on a 55-kb plasmid. The lcnA gene was cloned and sequenced. The sequence data revealed that LCN-A is ribosomally synthesized as a 75-amino-acid precursor including a 21-amino-acid N-terminal extension. An open reading frame encoding a 98-amino-acid polypeptide was found downstream of and in the same operon as lcnA. We propose that this open reading frame encodes an immunity function for LCN-A. In Escherichia coli lcnA did not cause an LCN-A+ phenotype. L. lactis subsp. lactis IL 1403 produced small amounts of the bacteriocin and became resistant to LCN-A after transformation with a recombinant plasmid carrying lcnA. The other lactococcal strains transformed with the same recombinant plasmid became resistant to LCN-A but did not produce any detectable amount of the bacteriocin.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Holo H,Nilssen O,Nes IF

doi

10.1128/jb.173.12.3879-3887.1991

subject

Has Abstract

pub_date

1991-06-01 00:00:00

pages

3879-87

issue

12

eissn

0021-9193

issn

1098-5530

journal_volume

173

pub_type

杂志文章
  • BamE modulates the Escherichia coli beta-barrel assembly machine component BamA.

    abstract::Biogenesis of the outer membrane (OM) is an essential process in gram-negative bacteria. One of the key steps of OM biogenesis is the assembly of integral outer membrane beta-barrel proteins (OMPs) by a protein machine called the Bam complex. In Escherichia coli, the Bam complex is composed of the essential proteins B...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06426-11

    authors: Rigel NW,Schwalm J,Ricci DP,Silhavy TJ

    更新日期:2012-03-01 00:00:00

  • Isolation and characterization of a gene from Streptomyces sp. strain C5 that confers the ability to convert daunomycin to doxorubicin on Streptomyces lividans TK24.

    abstract::DNA sequence analysis of a region of the Streptomyces sp. strain C5 daunomycin biosynthesis gene cluster, located between the daunomycin polyketide biosynthesis gene cluster and a dnrI (transcriptional activator) homolog, revealed the presence of a gene encoding a P-450-like enzyme with a deduced Mr of 46,096. Express...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.11.3389-3395.1996

    authors: Dickens ML,Strohl WR

    更新日期:1996-06-01 00:00:00

  • Identification of staphylococcal hemolysins by an electrophoretic localization technique.

    abstract::A technique for identifying and characterizing staphylococcal hemolysins by first separating them electrophoretically in barbital-buffered agar gel (pH 8.4) at 5 ma/cm for 2 hr and then determining their hemolytic activities by exposing them to human, horse, rabbit, and sheep erythrocytes is described. The alpha-hemol...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.2.525-530.1967

    authors: Haque RU

    更新日期:1967-02-01 00:00:00

  • Actin-binding proteins from Burkholderia mallei and Burkholderia thailandensis can functionally compensate for the actin-based motility defect of a Burkholderia pseudomallei bimA mutant.

    abstract::Recently we identified a bacterial factor (BimA) required for actin-based motility of Burkholderia pseudomallei. Here we report that Burkholderia mallei and Burkholderia thailandensis are capable of actin-based motility in J774.2 cells and that BimA homologs of these bacteria can restore the actin-based motility defec...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.22.7857-7862.2005

    authors: Stevens JM,Ulrich RL,Taylor LA,Wood MW,Deshazer D,Stevens MP,Galyov EE

    更新日期:2005-11-01 00:00:00

  • Intragenic recombination and a chimeric outer membrane protein in the relapsing fever agent Borrelia hermsii.

    abstract::The spirochete Borrelia hermsii, a relapsing fever agent, evades the host's immune response through multiphasic antigenic variation. Antigen switching results from sequential expression of genes for serotype-specific outer membrane proteins known as variable major proteins (Vmp's); of the 25 serotypes that have been i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.9.2516-2522.1993

    authors: Kitten T,Barrera AV,Barbour AG

    更新日期:1993-05-01 00:00:00

  • Soluble cytochromes from the marine methanotroph Methylomonas sp. strain A4.

    abstract::Soluble c-type cytochromes are central to metabolism of C1 compounds in methylotrophic bacteria. In order to characterize the role of c-type cytochromes in methane-utilizing bacteria (methanotrophs), we have purified four different cytochromes, cytochromes c-554, c-553, c-552, and c-551, from the marine methanotroph M...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.9.5360-5367.1990

    authors: DiSpirito AA,Lipscomb JD,Lidstrom ME

    更新日期:1990-09-01 00:00:00

  • Simple method for identification of plasmid-coded proteins.

    abstract::Proteins encoded by plasmid DNA are specifically labeled in UV-irradiated cells of Escherichia coli carrying recA and uvrA mutations because extensive degradation of the chromosome DNA occurs concurrently with amplification of plasmid DNA. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.137.1.692-693.1979

    authors: Sancar A,Hack AM,Rupp WD

    更新日期:1979-01-01 00:00:00

  • Regulation of a common amidotransferase subunit.

    abstract::In Bacillus subtilis the trpX locus specifies a glutamine-binding protein designated subunit X, which forms a complex with subunit E to constitute the anthranilate synthase enzyme aggregate (EX) and subunit A to constitute the p-aminobenzoate synthase enzyme aggregate (AX). Subunit X confers upon these enzyme complexe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.132.2.419-425.1977

    authors: Kane JF

    更新日期:1977-11-01 00:00:00

  • Characterization of the Pseudomonas sp. strain P51 gene tcbR, a LysR-type transcriptional activator of the tcbCDEF chlorocatechol oxidative operon, and analysis of the regulatory region.

    abstract::Plasmid pP51 of Pseudomonas sp. strain P51 contains two gene clusters encoding the degradation of chlorinated benzenes, tcbAB and tcbCDEF. A regulatory gene, tcbR, was located upstream and divergently transcribed from the chlorocatechol oxidative gene cluster tcbCDEF. The tcbR gene was characterized by DNA sequencing ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.12.3700-3708.1991

    authors: van der Meer JR,Frijters AC,Leveau JH,Eggen RI,Zehnder AJ,de Vos WM

    更新日期:1991-06-01 00:00:00

  • Secreted-protein response to sigmaU activity in Streptomyces coelicolor.

    abstract::The filamentous bacterium Streptomyces coelicolor forms an aerial mycelium as a prerequisite to sporulation, which occurs in the aerial hyphae. Uncontrolled activity of the extracytoplasmic function sigma factor sigmaU blocks the process of aerial mycelium formation in this organism. Using a green fluorescent protein ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01759-07

    authors: Gordon ND,Ottaviano GL,Connell SE,Tobkin GV,Son CH,Shterental S,Gehring AM

    更新日期:2008-02-01 00:00:00

  • Genetic requirements for potassium ion-dependent colony spreading in Bacillus subtilis.

    abstract::Undomesticated strains of Bacillus subtilis exhibit extensive colony spreading on certain soft agarose media: first the formation of dendritic clusters of cells, followed by spreading (pellicle-like) growth to cover the entire surface. These phases of colonization are dependent on the level of potassium ion (K(+)) but...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.24.8462-8469.2005

    authors: Kinsinger RF,Kearns DB,Hale M,Fall R

    更新日期:2005-12-01 00:00:00

  • Dual negative control of spx transcription initiation from the P3 promoter by repressors PerR and YodB in Bacillus subtilis.

    abstract::The spx gene encodes an RNA polymerase-binding protein that exerts negative and positive transcriptional control in response to oxidative stress in Bacillus subtilis. It resides in the yjbC-spx operon and is transcribed from at least five promoters located in the yjbC regulatory region or in the yjbC-spx intergenic re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01520-06

    authors: Leelakriangsak M,Kobayashi K,Zuber P

    更新日期:2007-03-01 00:00:00

  • Release of respiratory control in Escherichia coli after bacteriophage adsorption: process independent of DNA injection.

    abstract::Adsorption of phages T4, T5, and BF23 to previously starved Escherichia coli cells triggered the immediate release of respiratory control. A similar stimulation of respiration was induced after T4 ghost attachment, showing that this process was independent of the mechanism of DNA injection. Rather, this change in the ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.161.1.179-182.1985

    authors: Letellier L,Labedan B

    更新日期:1985-01-01 00:00:00

  • Escherichia coli proteins inducible by oxidative stress mediated by the superoxide radical.

    abstract::Two-dimensional gel analyses were made of proteins synthesized in Escherichia coli during various O2- -generating conditions. Nine proteins were constitutively synthesized over wild-type levels in superoxide dismutase (sodA sodB) double mutants. Addition of redox cycling agents such as paraquat and plumbagin at variou...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.3.1476-1484.1989

    authors: Walkup LK,Kogoma T

    更新日期:1989-03-01 00:00:00

  • Assimilatory sulfur metabolism in marine microorganisms: characteristics and regulation of sulfate transport in Pseudomonas halodurans and Alteromonas luteo-violaceus.

    abstract::Sulfate transport capacity was not regulated by cysteine, methionine, or glutathione in Pseudomonas halodurans, but growth on sulfate or thiosulfate suppressed transport. Subsequent sulfur starvation of cultures grown on all sulfur sources except glutathione stimulated uptake. Only methionine failed to regulate sulfat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.147.2.340-349.1981

    authors: Cuhel RL,Taylor CD,Jannasch HW

    更新日期:1981-08-01 00:00:00

  • Overexpression of vsr in Escherichia coli is mutagenic.

    abstract::Overexpression of vsr in Escherichia coli stimulates transition and frameshift mutations. The pattern of mutations suggests that mutagenesis is due to saturation or inactivation of dam-directed mismatch repair. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.14.4294-4296.1996

    authors: Doiron KM,Viau S,Koutroumanis M,Cupples CG

    更新日期:1996-07-01 00:00:00

  • Degradation of 2,4,6-trichlorophenol by Phanerochaete chrysosporium: involvement of reductive dechlorination.

    abstract::Under secondary metabolic conditions, the lignin-degrading basidiomycete Phanerochaete chrysosporium mineralizes 2,4, 6-trichlorophenol. The pathway for the degradation of 2,4, 6-trichlorophenol has been elucidated by the characterization of fungal metabolites and oxidation products generated by purified lignin peroxi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.19.5159-5164.1998

    authors: Reddy GV,Gelpke MD,Gold MH

    更新日期:1998-10-01 00:00:00

  • Cloning and nucleotide sequencing of Rhizobium meliloti aminotransferase genes: an aspartate aminotransferase required for symbiotic nitrogen fixation is atypical.

    abstract::In Rhizobium meliloti, an aspartate aminotransferase (AspAT) encoded within a 7.3-kb HindIII fragment was previously shown to be required for symbiotic nitrogen fixation and aspartate catabolism (V. K. Rastogi and R.J. Watson, J. Bacteriol. 173:2879-2887, 1991). A gene coding for an aromatic aminotransferase located w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.7.1919-1928.1993

    authors: Watson RJ,Rastogi VK

    更新日期:1993-04-01 00:00:00

  • AggR, a transcriptional activator of aggregative adherence fimbria I expression in enteroaggregative Escherichia coli.

    abstract::Enteroaggregative Escherichia coli (EAggEC) has been associated with persistent pediatric diarrhea in the developing world, yet the pathogenetic mechanisms of EAggEC infection are unknown. Our previous data have suggested that aggregative adherence of some EAggEC strains to HEp-2 cells is mediated by flexible, bundle-...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.15.4691-4699.1994

    authors: Nataro JP,Yikang D,Yingkang D,Walker K

    更新日期:1994-08-01 00:00:00

  • Microtubular structures in a stable staphylococcal L-form.

    abstract::Microtubular structures, which were demonstrated as straight, dense-walled cylinders attached to cell membranes, were found in a stable staphylococcal L-form grown in the absence of antibiotic. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.132.3.1024-1026.1977

    authors: Eda T,Kanda Y,Mori C,Kimura S

    更新日期:1977-12-01 00:00:00

  • Mismatch correction in pneumococcal transformation: donor length and hex-dependent marker efficiency.

    abstract::A hypothesis that preferential rejection of donor markers by the hex system of pneumococcus is due to lethal double-strand breaks has been examined in terms of its implications for the extent of the excision required. Experiments reported here were directed at asking whether hex-dependent marker efficiency depends on ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.125.1.125-135.1976

    authors: Guild WR,Shoemaker NB

    更新日期:1976-01-01 00:00:00

  • Staphylococcal Muller phenomenon: relationship to the plasminogen-plasmin system.

    abstract::Quie, Paul G. (University of Minnesota, Minneapolis) and Lewis W. Wannamaker. Staphylococcal Muller phenomenon: relationship to the plasminogen-plasmin system. J. Bacteriol. 82:770-783. 1961.-The staphylococcal factor that produces particulate proteolysis (Muller phenomenon) in whole blood or hemoglobinserum agar plat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.82.5.770-783.1961

    authors: QUIE PG,WANNAMAKER LW

    更新日期:1961-11-01 00:00:00

  • Identification of active site residues by site-directed mutagenesis of delta 5-3-ketosteroid isomerase from Pseudomonas putida biotype B.

    abstract::In order to assess the roles of specific amino acid residues in the delta 5-3-ketosteroid isomerase from Pseudomonas putida biotype B during catalysis, we replaced aspartic acid 40 with asparagine (D40N) and tyrosine 16 with phenylalanine (Y16F) in the enzyme by site-directed mutagenesis. Both purified mutant enzymes ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.9.2602-2605.1995

    authors: Kim SW,Choi KY

    更新日期:1995-05-01 00:00:00

  • A dominant-negative fur mutation in Bradyrhizobium japonicum.

    abstract::In many bacteria, the ferric uptake regulator (Fur) protein plays a central role in the regulation of iron uptake genes. Because iron figures prominently in the agriculturally important symbiosis between soybean and its nitrogen-fixing endosymbiont Bradyrhizobium japonicum, we wanted to assess the role of Fur in the i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.5.1409-1414.2004

    authors: Benson HP,LeVier K,Guerinot ML

    更新日期:2004-03-01 00:00:00

  • Mutations to spectinomycin resistance which alleviate the restriction of an amber suppressor by streptomycin resistance.

    abstract::Unlike mutations to streptomycin resistance, which restrict the function of an SU II amber suppressor, mutations to spectinomycin resistance have no restrictive effect. Furthermore, mutations to spectinomycin resistance counteract the restrictive action of streptomycin-resistant mutations when combined in a single str...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.97.2.940-943.1969

    authors: Kuwano M,Endo H,Ohnishi Y

    更新日期:1969-02-01 00:00:00

  • Suppressor analysis reveals a role for SecY in the SecA2-dependent protein export pathway of Mycobacteria.

    abstract::All bacteria use the conserved Sec pathway to transport proteins across the cytoplasmic membrane, with the SecA ATPase playing a central role in the process. Mycobacteria are part of a small group of bacteria that have two SecA proteins: the canonical SecA (SecA1) and a second, specialized SecA (SecA2). The SecA2-depe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00630-13

    authors: Ligon LS,Rigel NW,Romanchuk A,Jones CD,Braunstein M

    更新日期:2013-10-01 00:00:00

  • Isolation of catalase-deficient Escherichia coli mutants and genetic mapping of katE, a locus that affects catalase activity.

    abstract::A number of catalase-deficient mutants of Escherichia coli which exhibit no assayable catalase activity were isolated. The only physiological difference between the catalase mutants and their parents was a 50- to 60-fold greater sensitivity to killing by hydrogen peroxide. For comparison, mutations in the xthA and rec...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.157.2.622-626.1984

    authors: Loewen PC

    更新日期:1984-02-01 00:00:00

  • Novel pathway for conversion of chlorohydroxyquinol to maleylacetate in Burkholderia cepacia AC1100.

    abstract::Burkholderia cepacia AC1100 metabolizes 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) via formation of 5-chlorohydroxyquinol (5-CHQ), hydroxyquinol (HQ), maleylacetate, and beta-oxoadipate. The step(s) leading to the dechlorination of 5-CHQ to HQ has remained unidentified. We demonstrate that a dechlorinating enzyme, Tf...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.17.4667-4675.1998

    authors: Zaborina O,Daubaras DL,Zago A,Xun L,Saido K,Klem T,Nikolic D,Chakrabarty AM

    更新日期:1998-09-01 00:00:00

  • Polynucleotide phosphorylase plays an important role in the generation of spontaneous mutations in Escherichia coli.

    abstract::Polynucleotide phosphorylase (PNP) plays a central role in RNA degradation, generating a pool of ribonucleoside diphosphates (rNDPs) that can be converted to deoxyribonucleoside diphosphates (dNDPs) by ribonucleotide reductase. We report here that spontaneous mutations resulting from replication errors, which are norm...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00962-12

    authors: Becket E,Tse L,Yung M,Cosico A,Miller JH

    更新日期:2012-10-01 00:00:00

  • Cloning, sequencing, and expression of ruvB and characterization of RuvB proteins from two distantly related thermophilic eubacteria.

    abstract::The ruvB genes of the highly divergent thermophilic eubacteria Thermus thermophilus and Thermotoga maritima were cloned, sequenced, and expressed in Escherichia coli. Both thermostable RuvB proteins were purified to homogeneity. Like E. coli RuvB protein, both purified thermostable RuvB proteins showed strong double-s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.9.2695-2700.1996

    authors: Tong J,Wetmur JG

    更新日期:1996-05-01 00:00:00