Polynucleotide phosphorylase plays an important role in the generation of spontaneous mutations in Escherichia coli.

Abstract:

:Polynucleotide phosphorylase (PNP) plays a central role in RNA degradation, generating a pool of ribonucleoside diphosphates (rNDPs) that can be converted to deoxyribonucleoside diphosphates (dNDPs) by ribonucleotide reductase. We report here that spontaneous mutations resulting from replication errors, which are normally repaired by the mismatch repair (MMR) system, are sharply reduced in a PNP-deficient Escherichia coli strain. This is true for base substitution mutations that occur in the rpoB gene leading to Rif(r) and the gyrB gene leading to Nal(r) and for base substitution and frameshift mutations that occur in the lacZ gene. These results suggest that the increase in the rNDP pools generated by polynucleotide phosphorylase (PNP) degradation of RNA is responsible for the spontaneous mutations observed in an MMR-deficient background. The PNP-derived pool also appears responsible for the observed mutations in the mutT mutator background and those that occur after treatment with 5-bromodeoxyuridine, as these mutations are also drastically reduced in a PNP-deficient strain. However, mutation frequencies are not reduced in a mutY mutator background or after treatment with 2-aminopurine. These results highlight the central role in mutagenesis played by the rNDP pools (and the subsequent dNTP pools) derived from RNA degradation.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Becket E,Tse L,Yung M,Cosico A,Miller JH

doi

10.1128/JB.00962-12

subject

Has Abstract

pub_date

2012-10-01 00:00:00

pages

5613-20

issue

20

eissn

0021-9193

issn

1098-5530

pii

JB.00962-12

journal_volume

194

pub_type

杂志文章
  • A role for the EAL-like protein STM1344 in regulation of CsgD expression and motility in Salmonella enterica serovar Typhimurium.

    abstract::The bacterial second messenger cyclic di-GMP (c-di-GMP) regulates the transition between sessility and motility. In Salmonella enterica serovar Typhimurium, the expression of CsgD, the regulator of multicellular rdar morphotype behavior, is a major target of c-di-GMP signaling. CsgD expression is positively regulated ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00290-09

    authors: Simm R,Remminghorst U,Ahmad I,Zakikhany K,Römling U

    更新日期:2009-06-01 00:00:00

  • Isolation and characterization of a sporulation initiation mutation in the Bacillus subtilis secA gene.

    abstract::A Bacillus subtilis secA mutant, secA12, which is blocked at an early stage of sporulation, is able to grow as well as the wild-type strain at all temperatures tested. Experiments with lacZ fusion genes showed that the induction of kinA expression, as well as the sporulation-specific transcription of the spo0A gene, w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.2.544-547.1997

    authors: Asai K,Kawamura F,Sadaie Y,Takahashi H

    更新日期:1997-01-01 00:00:00

  • Purification and properties of Myxococcus xanthus cell surface antigen 1604.

    abstract::A cell surface antigen complex from Zwittergent-solubilized Myxococcus xanthus has been purified by immunoaffinity chromatography with monoclonal antibody (MAb) 1604 and by subsequent gel filtration. We propose that the cell surface antigen (CSA) 1604 complex participates in intercellular interactions. The apparent to...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.9.4655-4666.1989

    authors: Jarvis BW,Dworkin M

    更新日期:1989-09-01 00:00:00

  • Functional organization of the autotransporter adhesin involved in diffuse adherence.

    abstract::The Escherichia coli adhesin involved in diffuse adherence (AIDA-I) is a multifunctional autotransporter protein that mediates bacterial aggregation and biofilm formation, as well as adhesion and invasion of cultured epithelial cells. To elucidate the structure-function relationships of AIDA-I, we performed transposon...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01238-07

    authors: Charbonneau ME,Mourez M

    更新日期:2007-12-01 00:00:00

  • Activation of the lac genes of Tn951 by insertion sequences from Pseudomonas cepacia.

    abstract::We have identified three transposable gene-activating elements from Pseudomonas cepacia on the basis of their abilities to increase expression of the lac genes of the broad-host-range plasmid pGC91.14 (pRP1::Tn951). When introduced into auxotrophic derivatives of P. cepacia 249 (ATCC 17616), this plasmid failed to con...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.4.1719-1724.1990

    authors: Wood MS,Lory C,Lessie TG

    更新日期:1990-04-01 00:00:00

  • Functions in outer and inner membranes of Escherichia coli for ferrichrome transport.

    abstract::Mutants of the fhuA gene of Escherichia coli K-12, which encodes a receptor protein in the outer membrane, took up ferrichrome after exposure to pronase, whereas fhuB mutants remained transport negative. The latter finding supports our previous proposal that fhuB mutants are defective in a function that residues in th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.146.3.1158-1161.1981

    authors: Wookey PJ,Hussein S,Braun V

    更新日期:1981-06-01 00:00:00

  • Analysis of the σE regulon in Crohn's disease-associated Escherichia coli revealed involvement of the waaWVL operon in biofilm formation.

    abstract:UNLABELLED:Ileal lesions of patients with Crohn's disease are colonized by adherent-invasive Escherichia coli (AIEC), which is able to adhere to and to invade intestinal epithelial cells (IEC), to replicate within macrophages, and to form biofilms on the surface of the intestinal mucosa. Previous analyses indicated the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02499-14

    authors: Chassaing B,Garénaux E,Carriere J,Rolhion N,Guérardel Y,Barnich N,Bonnet R,Darfeuille-Michaud A

    更新日期:2015-04-01 00:00:00

  • Characterization of pyridoxine auxotrophs of Escherichia coli: serine and pdxF mutants.

    abstract::At least six phenotypically distinct classes of mutants of Escherichia coli which require serine or pyridoxine or both can be isolated. Three of the six classes lack 3-phosphoserine-2-oxoglutarate aminotransferase. One of these classes contains WG5, a mutant previously characterized as containing the pdxF5 allele. The...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.104.2.658-667.1970

    authors: Dempsey WB,Ito H

    更新日期:1970-11-01 00:00:00

  • Rotation and switching of the flagellar motor assembly in Halobacterium halobium.

    abstract::Halobacterium halobium swims with a polarly inserted motor-driven flagellar bundle. The swimming direction of the cell can be reserved by switching the rotational sense of the bundle. The switch is under the control of photoreceptor and chemoreceptor proteins that act through a branched signal chain. The swimming beha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.6.1971-1977.1991

    authors: Marwan W,Alam M,Oesterhelt D

    更新日期:1991-03-01 00:00:00

  • Partial characterization of lipid A of intraperiplasmically grown Bdellovibrio bacteriovorus.

    abstract::The lipid A components of substrate cell origin incorporated by Bdellovibrio bacteriovorus during intraperiplasmic growth (D. R. Nelson and S. C. Rittenberg, J. Bacteriol. 147:860-868, 1981) were shown to be integrated into its lipopolysaccharide structure. Lipid A isolated from bdellovibrios grown on Escherichia coli...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.147.3.869-874.1981

    authors: Nelson DR,Rittenberg SC

    更新日期:1981-09-01 00:00:00

  • Genetic analysis of the Sinorhizobium meliloti BacA protein: differential effects of mutations on phenotypes.

    abstract::Sinorhizobium meliloti strains lacking BacA function are impaired in symbiosis with alfalfa host plants and display altered sensitivities to a number of compounds relative to wild-type strains. With the goal of finding clues to the currently unknown biological function(s) of BacA, we carried out a genetic analysis to ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.21.6444-6453.2001

    authors: LeVier K,Walker GC

    更新日期:2001-11-01 00:00:00

  • Gene structures and regulation of the alkane hydroxylase complex in Acinetobacter sp. strain M-1.

    abstract::In the long-chain n-alkane degrader Acinetobacter sp. strain M-1, two alkane hydroxylase complexes are switched by controlling the expression of two n-alkane hydroxylase-encoding genes in response to the chain length of n-alkanes, while rubredoxin and rubredoxin ruductase are encoded by a single gene and expressed con...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.5.1819-1823.2001

    authors: Tani A,Ishige T,Sakai Y,Kato N

    更新日期:2001-03-01 00:00:00

  • Physical and functional interactions between Escherichia coli MutY glycosylase and mismatch repair protein MutS.

    abstract::Escherichia coli MutY and MutS increase replication fidelity by removing adenines that were misincorporated opposite 7,8-dihydro-8-oxo-deoxyguanines (8-oxoG), G, or C. MutY DNA glycosylase removes adenines from these mismatches through a short-patch base excision repair pathway and thus prevents G:C-to-T:A and A:T-to-...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01513-06

    authors: Bai H,Lu AL

    更新日期:2007-02-01 00:00:00

  • A cryptic promoter in the O(R) region of bacteriophage lambda.

    abstract::A cryptic promoter, designated P alpha, initiates transcription within the O(R) region of bacteriophage lambda. Transcription from P alpha proceeds in the direction of the cI repressor gene from sites 46 and 48 bp preceding the PRM transcription start site. P alpha is likely to compete with both PR and PRM for formati...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.17.5648-5654.1993

    authors: Woody ST,Fong RS,Gussin GN

    更新日期:1993-09-01 00:00:00

  • Metabolism of nicotinamide adenine dinucleotide in human and bovine strainsof Mycobacterium tuberculosis.

    abstract::A marked difference was found to exist between the nicotinamide adenine dinucleotide (NAD) glycohydrolase activity of human strains of Mycobacterium tuberculosis as compared with bovine strains. Human strains had from 6- to 20-fold higher NAD glycohydrolase activity than bovine strains. This finding explains the accum...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.110.2.600-603.1972

    authors: Kasărov LB,Moat AG

    更新日期:1972-05-01 00:00:00

  • Identification of a novel matrix protein that promotes biofilm maturation in Vibrio fischeri.

    abstract::Bacteria form communities, termed biofilms, in which cells adhere to each other within a matrix, typically comprised of polysaccharides, proteins, and extracellular DNA. Biofilm formation by the marine bacterium Vibrio fischeri requires the Syp polysaccharide, but the involvement of matrix proteins is as yet unknown. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02292-14

    authors: Ray VA,Driks A,Visick KL

    更新日期:2015-02-01 00:00:00

  • Type 1 fimbriae contribute to catheter-associated urinary tract infections caused by Escherichia coli.

    abstract::Biofilm formation on catheters is thought to contribute to persistence of catheter-associated urinary tract infections (CAUTI), which represent the most frequent nosocomial infections. Knowledge of genetic factors for catheter colonization is limited, since their role has not been assessed using physicochemical condit...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00985-13

    authors: Reisner A,Maierl M,Jörger M,Krause R,Berger D,Haid A,Tesic D,Zechner EL

    更新日期:2014-03-01 00:00:00

  • Fatty acid replacements in a fatty acid auxotroph of Escherichia coli.

    abstract::Unsaturated fatty acids having structural features which are different from those of the monoenoic acids normally synthesized by Escherichia coli can serve as growth factors for an auxotroph requiring unsaturated fatty acids. These analogues were incorporated into the phospholipids, as shown by gas-liquid and thin-lay...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.5.1658-1665.1968

    authors: Silbert DF,Ruch F,Vagelos PR

    更新日期:1968-05-01 00:00:00

  • Two gene determinants are differentially involved in the biogenesis of Fap1 precursors in Streptococcus parasanguis.

    abstract::Mature Fap1, a 200-kDa fimbria-associated adhesin, is required for fimbrial biogenesis and biofilm formation in Streptococcus parasanguis. Fap1-like proteins are found in the genomes of many streptococcal and staphylococcal species. Fap1 is a serine-rich glycoprotein modified by O-linked glycan moieties. In this study...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00836-06

    authors: Wu H,Bu S,Newell P,Chen Q,Fives-Taylor P

    更新日期:2007-02-01 00:00:00

  • In vivo interaction of endotoxin with a plasma lipoprotein having esterase activity.

    abstract::Circulating endotoxin was specifically precipitated from plasma samples withdrawn from three different animal species subsequent to parenteral injection of the toxin. Lipoprotein-positive staining and esterase activity were demonstrated on the precipitation lines formed in immunodiffusion, thus establishing the in viv...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.6.2031-2034.1968

    authors: Skarnes RC

    更新日期:1968-06-01 00:00:00

  • Expanded metabolic reconstruction of Helicobacter pylori (iIT341 GSM/GPR): an in silico genome-scale characterization of single- and double-deletion mutants.

    abstract::Helicobacter pylori is a human gastric pathogen infecting almost half of the world population. Herein, we present an updated version of the metabolic reconstruction of H. pylori strain 26695 based on the revised genome annotation and new experimental data. This reconstruction, iIT341 GSM/GPR, represents a detailed rev...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.16.5818-5830.2005

    authors: Thiele I,Vo TD,Price ND,Palsson BØ

    更新日期:2005-08-01 00:00:00

  • Lethal gram-negative bacterial superinfection in Guinea pigs given bacitracin.

    abstract::Farrar, W. Edmund, Jr. (Walter Reed Army Institute of Research, Washington, D.C.), Thomas H. Kent, and Van B. Elliott. Lethal gram-negative bacterial superinfection in guinea pigs given bacitracin. J. Bacteriol. 92:496-501. 1966.-Oral administration of a single dose of bacitracin (either 2,000 or 10,000 units) was let...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.92.2.496-501.1966

    authors: Farrar WE,Kent TH,Elliott VB

    更新日期:1966-08-01 00:00:00

  • Proline transport and metabolism in Rickettsia prowazekii.

    abstract::Purified Rickettsia prowazekii cells were able to transport L-proline. The influx of this amino acid had a Kt of 14 microM and a Vmax of about 64 pmol/min per mg of protein. Proline could not be transported by heat-killed or metabolically poisoned rickettsiae or at 0 degrees C. The uptake of proline was linear for alm...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.158.2.460-463.1984

    authors: Winkler HH,Daugherty RM

    更新日期:1984-05-01 00:00:00

  • Genome sequences of Listeria monocytogenes strains J1816 and J1-220, associated with human outbreaks.

    abstract::Listeria monocytogenes has caused numerous human outbreaks. Here we report draft genomes of L. monocytogenes J1816 and J1-220, which belong to epidemic clones II and IV, respectively. Whole-genome sequence analysis of these strains provides a tool for studying the short-term evolution of these epidemic clones. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05048-11

    authors: Chen Y,Strain EA,Allard M,Brown EW

    更新日期:2011-07-01 00:00:00

  • Variation in levels of enzymes related to energy metabolism in alternative developmental pathways of Blastocladiella emersonii.

    abstract::The activities of phosphofructokinase (PFK), fructose diphosphatase (FDP), nicotinamide adenine dinucleotide (NAD) and NAD phosphate (NADP)-linked isocitrate dehydrogenases (IDHNAD, IDHNADP), two NAD-linked glutamate dehydrogenases (GDH1, GDH2), and isocitrate lyase were studied during the development of the two pheno...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.126.3.1075-1081.1976

    authors: Ingebretsen OC,Sanner T

    更新日期:1976-06-01 00:00:00

  • Characterization of the role of the ToxR-modulated outer membrane porins OmpU and OmpT in Vibrio cholerae virulence.

    abstract::ToxR, the transmembrane regulatory protein required for expression of virulence factors in the human diarrheal pathogen Vibrio cholerae, directly activates and represses the transcription of two outer membrane porins, OmpU and OmpT, respectively. In an attempt to dissect the role of the OmpU and OmpT porins in viabili...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.12.3652-3662.2001

    authors: Provenzano D,Lauriano CM,Klose KE

    更新日期:2001-06-01 00:00:00

  • Characterization of a CO: heterodisulfide oxidoreductase system from acetate-grown Methanosarcina thermophila.

    abstract::During the methanogenic fermentation of acetate by Methanosarcina thermophila, the CO dehydrogenase complex cleaves acetyl coenzyme A and oxidizes the carbonyl group (or CO) to CO2, followed by electron transfer to coenzyme M (CoM)-S-S-coenzyme B (CoB) and reduction of this heterodisulfide to HS-CoM and HS-CoB (A. P. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.22.6974-6979.1994

    authors: Peer CW,Painter MH,Rasche ME,Ferry JG

    更新日期:1994-11-01 00:00:00

  • INFECTIOUS BOVINE RHINOTRACHEITIS VIRUS REPLICATION, CYTOPATHOLOGY, AND PLAQUE FORMATION IN THE PRESENCE AND ABSENCE OF NUCLEIC ACID ANALOGUES.

    abstract::Stevens, Jack G. (University of Washington, Seattle), and Neal B. Groman. Infectious bovine rhinotracheitis virus replication, cytopathology, and plaque formation in the presence and absence of nucleic acid analogues. J. Bacteriol. 87:446-453. 1964.-Cytopathology induced by infectious bovine rhinotracheitis (IBR) viru...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.2.446-453.1964

    authors: STEVENS JG,GROMAN NB

    更新日期:1964-02-01 00:00:00

  • Persistent cyclic herpes simplex virus infection in vitro. IV. Changes in the severity of the infections in the presence of antibody.

    abstract::Hampar, Berge (National Institute of Dental Research, Bethesda, Md.). Persistent cyclic herpes simplex virus infection in vitro. IV. Changes in the severity of the infections in the presence of antibody. J. Bacteriol. 92:1741-1747. 1966.-The severity of persistent herpes simplex virus (HSV) infections maintained in th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.92.6.1741-1747.1966

    authors: Hampar B

    更新日期:1966-12-01 00:00:00

  • Complementation of a Rhodopseudomonas sphaeroides ribulose bisphosphate carboxylase-oxygenase regulatory mutant from a genomic library.

    abstract::A genomic library containing HindIII partial digests of Rhodopseudomonas sphaeroides HR DNA was constructed in the broad-host-range cosmid cloning vector pVK102. With a portion of this library as donor in complementation studies with the ribulose 1,5-bisphosphate carboxylase-oxygenase regulatory mutant R. sphaeroides ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.1.147-154.1985

    authors: Weaver KE,Tabita FR

    更新日期:1985-10-01 00:00:00