Isolation and characterization of a sporulation initiation mutation in the Bacillus subtilis secA gene.

Abstract:

:A Bacillus subtilis secA mutant, secA12, which is blocked at an early stage of sporulation, is able to grow as well as the wild-type strain at all temperatures tested. Experiments with lacZ fusion genes showed that the induction of kinA expression, as well as the sporulation-specific transcription of the spo0A gene, was not observed in the secA12 mutant. However, transcription of the spo0H gene (coding for sigmaH, which is required for the transcription of kinA and spo0A) and accumulation of the sigmaH protein were not affected in secA12. These results suggested that mutations in secA affect a factor required for efficient transcription of kinA as well as for the activation of the phosphorelay pathway.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Asai K,Kawamura F,Sadaie Y,Takahashi H

doi

10.1128/jb.179.2.544-547.1997

subject

Has Abstract

pub_date

1997-01-01 00:00:00

pages

544-7

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

179

pub_type

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