Fatty acid replacements in a fatty acid auxotroph of Escherichia coli.

Abstract:

:Unsaturated fatty acids having structural features which are different from those of the monoenoic acids normally synthesized by Escherichia coli can serve as growth factors for an auxotroph requiring unsaturated fatty acids. These analogues were incorporated into the phospholipids, as shown by gas-liquid and thin-layer chromatographic analysis of the phospholipid fatty acid composition. Some of these fatty acids were cisDelta(5)- and cis-Delta(9)-tetradecenoic, cis-Delta(11)-eicosenoic, cis,cis-Delta(11,14)-eicosadienoic, cis,cis,cis-Delta(11,14,17)-eicosatrienoic, trans-Delta(9)- and trans-Delta(11)-octadecenoic acids. Although partial degradation of some of these analogues to shorter even-chain homologues occurred, chain elongation of the exogenous fatty acids was not detected. Trans-olefinic acids were utilized without stereochemical or positional isomerization. These studies provide a basis for exploring the properties of the fatty acids and phospholipids required for the formation, structure, and function of membranes.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Silbert DF,Ruch F,Vagelos PR

doi

10.1128/JB.95.5.1658-1665.1968

subject

Has Abstract

pub_date

1968-05-01 00:00:00

pages

1658-65

issue

5

eissn

0021-9193

issn

1098-5530

journal_volume

95

pub_type

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