Purification, characterization, and regulation of a nicotinamide adenine dinucleotide-dependent lactate dehydrogenase from Actinomyces viscosus.

abstract：:Salmonella virulence requires the initial invasion of host cells, followed by modulation of the intracellular environment for survival and replication. In an effort to characterize the role of small RNAs in Salmonella pathogenesis, we inadvertently identified a 5-kDa protein named YshB that is involved in the intracel...

journal_title：Journal of bacteriology

authors： Bomjan R,Zhang M,Zhou D

更新日期：2019-08-08 00:00:00

abstract：:Flagellum formation by ubiquinone- and menaquinone-deficient mutant strains of Escherichia coli K-12 was studied under both aerobic and anaerobic growth conditions. Ubiquinone was found to be obligatory for aerobic flagellum formation but could be replaced by menaquinone for anaerobic flagellum formation. A mutant dev...

journal_title：Journal of bacteriology

authors： Hertz R,Bar-Tana J

更新日期：1977-12-01 00:00:00

abstract：:Growth of Salmonella typhimurium on acetate as a sole carbon source requires expression of the glyoxylate shunt; however, the genes for the glyoxylate shunt enzymes have not been previously identified in S. typhimurium. In this study, we isolated transposon insertions in the genes for the two unique enzymes of this pa...

journal_title：Journal of bacteriology

authors： Wilson RB,Maloy SR

更新日期：1987-07-01 00:00:00

abstract：:ULTRACENTRIFUGATION IN SUCROSE DENSITY GRADIENTS WAS EMPLOYED TO ESTIMATE THE MOLECULAR WEIGHTS AND TO DETERMINE POSSIBLE PHYSICAL AGGREGATION OF THE FIVE ENZYMES CATALYZING STEPS TWO TO SIX IN THE PRECHORISMIC ACID PORTION OF THE POLYAROMATIC SYNTHETIC PATHWAY IN SIX SPECIES OF BACTERIA: Escherichia coli, Salmonella ...

journal_title：Journal of bacteriology

authors： Berlyn MB,Giles NH

更新日期：1969-07-01 00:00:00

abstract：:The F-plasmid-encoded TraI protein, also known as DNA helicase I, is a bifunctional protein required for conjugative DNA transfer. The enzyme catalyzes two distinct but functionally related reactions required for the DNA processing events associated with conjugation: the site- and strand-specific transesterification (...

journal_title：Journal of bacteriology

authors： Matson SW,Ragonese H

更新日期：2005-01-01 00:00:00

abstract：:The D1:1 protein and its potentially occurring degradation products were overexpressed in Escherichia coli. Protein-DNA interaction is shown for the promoter region of psbAI. The D1:1 degradation products may be involved in transcription regulation of psbAI by binding in the promoter region. Additionally, C-terminal f...

journal_title：Journal of bacteriology

authors： Stelljes C,Koenig F

更新日期：2007-03-01 00:00:00

abstract：:Cells of the cyanobacterium Anabaena variabilis starved for phosphate for 3 days took up phosphate at about 100 times the rate of unstarved cells. Kinetic data suggested that a new transport system had been induced by starvation for phosphate. The inducible phosphate transport system was quickly repressed by addition ...

journal_title：Journal of bacteriology

authors： Thiel T

更新日期：1988-03-01 00:00:00

abstract：:In Staphylococcus aureus, the transport of dicarboxylates is mediated in part by the Na+-linked carrier protein SdcS. This transporter is a member of the divalent-anion/Na+ symporter (DASS) family, a group that includes the mammalian Na+/dicarboxylate cotransporters NaDC1 and NaDC3. In earlier work, we cloned and expr...

journal_title：Journal of bacteriology

authors： Hall JA,Pajor AM

更新日期：2007-02-01 00:00:00

abstract：:Single spores of Saccharomyces cerevisiae were examined during germination and outgrowth by scanning electron and phase-contrast microscopy. Also determined were changes in cell weight and light absorbance, trehalose utilization, and synthesis of protein and KOH-soluble carbohydrates. These studies reveal that develop...

journal_title：Journal of bacteriology

authors： Rousseau P,Halvorson HO,Bulla LA Jr,St Julian G

更新日期：1972-03-01 00:00:00

abstract：:The methylenetetrahydrofolate dehydrogenase of the amethopterin-resistant strain Streptococcus faecium var. durans A(k) was purified 100-fold. Because it is extremely labile, this enzyme required protection by 1 mm nicotinamide adenine dinucleotide phosphate (NADP(+)) during purification; 0.01 mm EADP(+) with 0.1% bov...

journal_title：Journal of bacteriology

authors： Albrecht AM,Pearce FK,Hutchison DJ

更新日期：1968-05-01 00:00:00

abstract：:Staphylococcus lugdunensis is the only coagulase-negative Staphylococcus species with a locus encoding iron-regulated surface determinant (Isd) proteins. In Staphylococcus aureus, the Isd proteins capture heme from hemoglobin and transfer it across the wall to a membrane-bound transporter, which delivers it into the c...

journal_title：Journal of bacteriology

authors： Zapotoczna M,Heilbronner S,Speziale P,Foster TJ

更新日期：2012-12-01 00:00:00

abstract：:The major cold shock protein of Escherichia coli, CS7.4, is produced at a level of 13% of total protein synthesis upon a temperature shift from 37 to 10 degrees C. The transcription of its gene (cspA) was found to be tightly regulated and induced only at low temperature. In addition, the cspA mRNA was extremely unstab...

journal_title：Journal of bacteriology

authors： Tanabe H,Goldstein J,Yang M,Inouye M

更新日期：1992-06-01 00:00:00

abstract：:A Mycoplasma gallisepticum subcellular fraction (P2), which contains the deoxyribonucleic acid replication complex, can be isolated by differential centrifugation of freeze-thaw-lysed cells. The nascent deoxyribonucleic acid is released from P2 by Lubrol-WX, sodium dodecyl sulfate, Pronase, and deoxyribonuclease, but ...

journal_title：Journal of bacteriology

authors： Maniloff J,Quinlan DC

更新日期：1974-10-01 00:00:00

abstract：:Mycoplasma laidlawii was recovered from burns from 13 of 52 patients. In most cases, cocci or bacilli were isolated simultaneously. ...

journal_title：Journal of bacteriology

authors： Markham JG,Markham NP

更新日期：1969-05-01 00:00:00

abstract：:Purine nucleoside phosphorylase (EC 2.4.2.1) from Bacillus cereus T was examined at hourly intervals during growth and sporulation. The enzyme has maximal activity in extracts prepared from cells during stages I and II. The activity during exponential growth is only 6.6% of the maximum and that in free spores is only ...

journal_title：Journal of bacteriology

authors： Englebrecht HL

更新日期：1972-07-01 00:00:00

abstract：:Rhythmically advancing waves of cells, called ripples, arise spontaneously during the aggregation of Myxococcus xanthus into fruiting bodies. Extracts prepared by washing rippling cells contain a substance that will induce quiescent cells to ripple. Three lines of evidence indicate that murein (peptidoglycan) is the r...

journal_title：Journal of bacteriology

authors： Shimkets LJ,Kaiser D

更新日期：1982-10-01 00:00:00

abstract：:Sato, Ichiei (School of Medicine, Gunma University, Maebashi, Japan), Tokumitsu Tanaka, Kazuko Saito, and Susumu Mitsuhashi. Inhibition of Salmonella enteritidis ingested in mononuclear phagocytes from liver and subcutaneous tissue of mice immunized with live vaccine. J. Bacteriol. 83:1306-1312. 1962.-In our earlier s...

journal_title：Journal of bacteriology

authors： SATO I,TANAKA T,SAITO K,MITSUHASHI S

更新日期：1962-06-01 00:00:00

abstract：:In response to molecular oxygen and/or fixed nitrogen, the product of the Klebsiella pneumoniae nitrogen fixation L (nifL) gene inhibits NifA-mediated transcriptional activation. Nitrogen regulation of NifL function occurs at two levels: transcription of the nifLA operon is regulated by the general Ntr system, and the...

journal_title：Journal of bacteriology

authors： He L,Soupene E,Kustu S

更新日期：1997-12-01 00:00:00

abstract：:Addition of purines to the growth medium of Escherichia coli represses synthesis of cytosine deaminase (codA) and enzymes of purine de novo synthesis. After Tn10 mutagenesis, mutants displaying derepressed levels of cytosine deaminase in the presence of hypoxanthine were isolated. One of these had simultaneously acqui...

journal_title：Journal of bacteriology

authors： Kilstrup M,Meng LM,Neuhard J,Nygaard P

更新日期：1989-04-01 00:00:00

abstract：:The murG gene of Escherichia coli encodes the N-acetylglucosaminyltransferase responsible for the final step in the formation of the lipid-linked disaccharide-pentapeptide subunit of peptidoglycan. Using trypsin to probe maxicell spheroplasts, we show that this enzyme is peripherally associated with the inner face of ...

journal_title：Journal of bacteriology

authors： Bupp K,van Heijenoort J

更新日期：1993-03-01 00:00:00

abstract：:Transcriptomic, metabolomic, physiological, and computational modeling approaches were integrated to gain insight into the mechanisms of antibiotic tolerance in an in vitro biofilm system. Pseudomonas aeruginosa biofilms were grown in drip flow reactors on a medium composed to mimic the exudate from a chronic wound. A...

journal_title：Journal of bacteriology

authors： Stewart PS,White B,Boegli L,Hamerly T,Williamson KS,Franklin MJ,Bothner B,James GA,Fisher S,Vital-Lopez FG,Wallqvist A

更新日期：2019-10-21 00:00:00

abstract：:The degradation of synthetic compounds requires bacteria to recruit and adapt enzymes from pathways for naturally occurring compounds. Previous work defined the steps in 2,4-dinitrotoluene (2,4-DNT) metabolism through the ring fission reaction. The results presented here characterize subsequent steps in the pathway th...

journal_title：Journal of bacteriology

authors： Johnson GR,Jain RK,Spain JC

更新日期：2002-08-01 00:00:00

abstract：:In this study, we cloned the Pseudomonas aeruginosa zwf gene, encoding glucose-6-phosphate dehydrogenase (G6PDH), an enzyme that catalyzes the NAD+- or NADP+-dependent conversion of glucose-6-phosphate to 6-phosphogluconate. The predicted zwf gene product is 490 residues, which could form a tetramer with a molecular m...

journal_title：Journal of bacteriology

authors： Ma JF,Hager PW,Howell ML,Phibbs PV,Hassett DJ

更新日期：1998-04-01 00:00:00

abstract：:The virulence factors of the cariogenic bacterium Streptococcus sobrinus have been difficult to assess because of a lack of tools for the genetic manipulation of this organism. The construction of an Escherichia coli-Streptococcus shuttle vector, pDL289, that can be mobilized into S. sobrinus by the conjugative plasmi...

journal_title：Journal of bacteriology

authors： Buckley ND,Lee LN,LeBlanc DJ

更新日期：1995-09-01 00:00:00

abstract：:Level of competence reached by Bacillus subtilis 168 lysogenic for temperate phage phi 105 was reduced compared to that reached by nonlysogenic cells. This effect was probably related to an alteration of the bacterial surface. Deoxyribonucleic acid extracted from phi 105 lysogenic bacteria was used to transform other ...

journal_title：Journal of bacteriology

authors： Peterson AM,Rutberg L

更新日期：1969-06-01 00:00:00

abstract：:Isolated cell walls of Staphylococcus aureus and Micrococcus lysodeikticus were found to expand and contract in response to changes in environmental pH and ionic strength. These volume changes, which could amount to as much as a doubling of wall dextran-impermeable volume, were related to changes in electrostatic inte...

journal_title：Journal of bacteriology

authors： Ou LT,Marquis RE

更新日期：1970-01-01 00:00:00

abstract：:Two-dimensional gel analyses were made of proteins synthesized in Escherichia coli during various O2- -generating conditions. Nine proteins were constitutively synthesized over wild-type levels in superoxide dismutase (sodA sodB) double mutants. Addition of redox cycling agents such as paraquat and plumbagin at variou...

journal_title：Journal of bacteriology

authors： Walkup LK,Kogoma T

更新日期：1989-03-01 00:00:00

abstract：:Thin pili of the closely related IncI1 plasmids ColIb-P9 and R64 are required only for liquid mating and belong to the type IV family of pili. They were sedimented by ultracentrifugation from culture medium in which Escherichia coli cells harboring ColIb-P9- or R64-derived plasmids had been grown, and then the pili we...

journal_title：Journal of bacteriology

authors： Yoshida T,Furuya N,Ishikura M,Isobe T,Haino-Fukushima K,Ogawa T,Komano T

更新日期：1998-06-01 00:00:00

abstract：:Here we have characterized the Rickettsia prowazekii RP534 protein, a homologue of the Pseudomonas aeruginosa ExoU phospholipase A (PLA) secreted cytotoxin. Our studies showed that purified recombinant RP534 PLA possessed the predicted PLA(2) and lyso-PLA(2) activities based on what has been published for P. aeruginos...

journal_title：Journal of bacteriology

authors： Housley NA,Winkler HH,Audia JP

更新日期：2011-09-01 00:00:00

abstract：:In Escherichia coli K-12, two enzymes, encoded by ilvE and tyrB, catalyze the amination of 2-ketoisocaproate (2-KIC) to form leucine. Although leucine-requiring derivatives of an ilvE strain that are unable to grow on 2-KIC were expected to have mutations only in tyrB, mapping studies showed that one such mutation was...

journal_title：Journal of bacteriology

authors： Vartak NB,Liu L,Wang BM,Berg CM

更新日期：1991-06-01 00:00:00