Linked transformation of bacterial and prophage markers in Bacillus subtilis 168 lysogenic for bacteriophage phi 105.

Abstract:

:Level of competence reached by Bacillus subtilis 168 lysogenic for temperate phage phi 105 was reduced compared to that reached by nonlysogenic cells. This effect was probably related to an alteration of the bacterial surface. Deoxyribonucleic acid extracted from phi 105 lysogenic bacteria was used to transform other lysogenic bacteria. About 25% linkage was found between the bacterial phe-1 marker and prophage marker ts N15. The order of a few prophage markers relative to phe-1 was established in three-factor crosses. The usefulness of this system for a study of the linkage between an integrated prophage genome and that of its host was discussed.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Peterson AM,Rutberg L

doi

10.1128/JB.98.3.874-877.1969

subject

Has Abstract

pub_date

1969-06-01 00:00:00

pages

874-7

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

98

pub_type

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