Trypsin mediates growth phase-dependent transcriptional tegulation of genes involved in biosynthesis of ruminococcin A, a lantibiotic produced by a Ruminococcus gnavus strain from a human intestinal microbiota.

abstract：:The sequencing of the EcoRI-HindIII fragment complementing mutations in the structural genes of the L-rhamnose regulon of Escherichia coli has permitted identification of the open reading frames corresponding to rhaB, rhaA, and rhaD. The deduced amino acid sequences gave a 425-amino-acid polypeptide corresponding to r...

journal_title：Journal of bacteriology

authors： Moralejo P,Egan SM,Hidalgo E,Aguilar J

更新日期：1993-09-01 00:00:00

abstract：:Bacteriocin JF246 has been purified from mitomycin C-induced Serratia marcescens cells by salt extraction, ammonium sulfate fractionation, and chromatography on QAE-Sephadex and SP-Sephadex. The purified material is homogeneous on polyacrylamide gel electrophoresis in the presence of 2% sodium dodecyl sulfate or 6 m u...

journal_title：Journal of bacteriology

authors： Foulds J

更新日期：1972-06-01 00:00:00

abstract：:Two related killer strains of Saccharomyces cerevisiae were mutagenized and screened for nonkiller variants. About 20% of the mutants derived from one strain lacked all detectable double-straned ribonucleic acid (dsRNA). About 70% of the mutants from the other strain lacked one of the dsRNA species normally associated...

journal_title：Journal of bacteriology

authors： Vodkin M

更新日期：1977-10-01 00:00:00

abstract：:The nickel-cobalt-cadmium resistance genes carried by plasmid pTOM9 of Alcaligenes xylosoxidans 31A are located on a 14.5-kb BamHI fragment. By random Tn5 insertion mutagenesis, the fragment was shown to contain two distinct nickel resistance loci, ncc and nre. The ncc locus causes a high-level combined nickel, cobalt...

journal_title：Journal of bacteriology

authors： Schmidt T,Schlegel HG

更新日期：1994-11-01 00:00:00

abstract：:Integrative and conjugative elements (ICEs), also called conjugative transposons, are genomic islands that excise, self-transfer by conjugation, and integrate in the genome of the recipient bacterium. The current investigation shows the intraspecies conjugative transfer of the first described ICEs in Streptococcus the...

journal_title：Journal of bacteriology

authors： Bellanger X,Roberts AP,Morel C,Choulet F,Pavlovic G,Mullany P,Decaris B,Guédon G

更新日期：2009-04-01 00:00:00

abstract：:Polysaccharide-containing material was located by histochemical staining on ultrathin sections of Bacillus cereus and several clostridial species during various phases of growth. These components were located along the cell wall in young vegetative cells and along the developing cortex in sporulating cells. In B. cere...

journal_title：Journal of bacteriology

authors： Walker PD,Short J

更新日期：1969-06-01 00:00:00

abstract：:In Escherichia coli K-12, expression of the lysU gene is regulated by the lrp gene product, as indicated by an increase in the level of lysyl-tRNA synthetase activity and LysU protein in an lrp mutant. Comparison of the patterns of protein expression visualized by two-dimensional gel electrophoresis indicated that Lys...

journal_title：Journal of bacteriology

authors： Lin R,Ernsting B,Hirshfield IN,Matthews RG,Neidhardt FC,Clark RL,Newman EB

更新日期：1992-05-01 00:00:00

abstract：:Legionella pneumophila expresses two peroxide-scavenging alkyl hydroperoxide reductase systems (AhpC1 and AhpC2D) that are expressed differentially during the bacterial growth cycle. Functional loss of the postexponentially expressed AhpC1 system is compensated for by increased expression of the exponentially expresse...

journal_title：Journal of bacteriology

authors： LeBlanc JJ,Brassinga AK,Ewann F,Davidson RJ,Hoffman PS

更新日期：2008-05-01 00:00:00

abstract：:The nucleotide sequence of a 23S rRNA gene of Campylobacter coli VC167 was determined. The primary sequence of the C. coli 23S rRNA was deduced, and a secondary-structure model was constructed. Comparison with Escherichia coli 23S rRNA showed a major difference in the C. coli rRNA at approximately position 1170 (E. co...

journal_title：Journal of bacteriology

authors： Trust TJ,Logan SM,Gustafson CE,Romaniuk PJ,Kim NW,Chan VL,Ragan MA,Guerry P,Gutell RR

更新日期：1994-08-01 00:00:00

abstract：:The Escherichia coli cell division protein FtsQ is a central component of the divisome. FtsQ is a bitopic membrane protein with a large C-terminal periplasmic domain. In this work we investigated the role of the transmembrane segment (TMS) that anchors FtsQ in the cytoplasmic membrane. A set of TMS mutants was made an...

journal_title：Journal of bacteriology

authors： Scheffers DJ,Robichon C,Haan GJ,den Blaauwen T,Koningstein G,van Bloois E,Beckwith J,Luirink J

更新日期：2007-10-01 00:00:00

abstract：:Zorbamycin (U-30,604E) induces rapid degradation of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) in Bacillus subtilis cells. DNA degradation is initiated first and is closely followed by the degradation of RNA. No interaction between isolated DNA and zorbamycin is observed. Nucleic acid and protein syntheses...

journal_title：Journal of bacteriology

authors： Reusser F

更新日期：1971-10-01 00:00:00

abstract：:Nisin is a pore-forming antimicrobial peptide. The capacity of nisin to induce transmembrane movement of a fluorescent phospholipid in lipid vesicles was investigated. Unilamellar phospholipid vesicles that contained a fluorescent phospholipid (1-acyl-2-(6-[(7-nitro-2-1, 3-benzoxadiazol-4-yl)amino]caproyl)-sn-glycero-...

journal_title：Journal of bacteriology

authors： Moll GN,Konings WN,Driessen AJ

更新日期：1998-12-01 00:00:00

abstract：:Phosphoenolpyruvate (PEP) carboxykinase was identified to be the only C3-carboxylating enzyme in Alcaligenes eutrophus. The enzyme requires GDP or inosine diphosphate (GTP or inosine triphosphate) for activity. Pyruvate- and other PEP-dependent CO2-fixing enzyme activities were not detected, regardless of whether the ...

journal_title：Journal of bacteriology

authors： Schobert P,Bowien B

更新日期：1984-07-01 00:00:00

abstract：:Bacteroides ruminicola B(1)4, a noncellulolytic rumen bacterium, produces an endoglucanase (carboxymethylcellulase [CMCase]) that is excreted into the culture supernatant. Cultures grown on glucose, fructose, maltose, mannose, and cellobiose had high specific activities of CMCase (greater than 3 mmol of reducing sugar...

journal_title：Journal of bacteriology

authors： Matsushita O,Russell JB,Wilson DB

更新日期：1990-07-01 00:00:00

abstract：:Nishida, S. (Kanazawa University, Kanazawa, Japan), and G. Nakagawara. Isolation of toxigenic strains of Clostridium novyi from soil. J. Bacteriol. 88:1636-1640. 1964.-The cultural conditions for toxin production by Clostridium novyi were investigated, and the optimal constituents of a proper medium were determined. A...

journal_title：Journal of bacteriology

authors： NISHIDA S,NAKAGAWARA G

更新日期：1964-12-01 00:00:00

abstract：:The recF gene of Escherichia coli is known to encode an Mr-40,000 protein that is involved in DNA recombinationa nd postreplication DNA repair. To characterize the role of the recF gene product in these processes, the recF gene was cloned downstream of a tac promoter to facilitate overproduction of the recF gene produ...

journal_title：Journal of bacteriology

authors： Griffin TJ 4th,Kolodner RD

更新日期：1990-11-01 00:00:00

abstract：:The rates of DNA synthesis during the cell-division cycle were measured in Myxococcus xanthus growing in three different media permitting a twofold variation in doubling time. In all three media, simple DNA cycles were observed. Synthesis of DNA occurred during 85% of the cell-division cycle, independent of generation...

journal_title：Journal of bacteriology

authors： Zusman DR,Krotoski DM,Cumsky M

更新日期：1978-01-01 00:00:00

abstract：:A systematic analysis of the type III secretion (T3S) genes of Pseudomonas aeruginosa strain PAO1 revealed that they are under quorum-sensing control. This observation was supported by the down-regulation of the T3S regulon in the presence of RhlR-C4HSL and the corresponding advanced secretion of ExoS in a rhlI mutant...

journal_title：Journal of bacteriology

authors： Bleves S,Soscia C,Nogueira-Orlandi P,Lazdunski A,Filloux A

更新日期：2005-06-01 00:00:00

abstract：:Spontaneous mutants of Staphylococcus aureus resistant to rifampin, rifamycin SV, streptovaricin, or streptolydigin were isolated and shown to be resistant due to chromosomal rather than plasmid mutations. Based on data concerning spontaneous mutation rates, genetic cotransduction rates, and in vitro sensitivity studi...

journal_title：Journal of bacteriology

authors： Morrow TO,Harmon SA

更新日期：1979-01-01 00:00:00

abstract：:Features of the amino acid sequence of the TnaC nascent peptide are recognized by the translating ribosome. Recognition leads to tryptophan binding within the translating ribosome, inhibiting the termination of tnaC translation and preventing Rho-dependent transcription termination in the tna operon leader region. It ...

journal_title：Journal of bacteriology

authors： Cruz-Vera LR,New A,Squires C,Yanofsky C

更新日期：2007-04-01 00:00:00

abstract：:The four methyl-accepting chemotaxis proteins of Escherichia coli, often called transducers, are transmembrane receptor proteins that exhibit substantial identity among the sequences of their cytoplasmic domains. Thus, antiserum raised to one of these proteins recognizes the others and might be expected to recognize r...

journal_title：Journal of bacteriology

authors： Morgan DG,Baumgartner JW,Hazelbauer GL

更新日期：1993-01-01 00:00:00

abstract：:Evidence for the existence of two molecular species of exfoliative toxin (ET) synthesized by Staphylococcus hyicus (SHET) under chromosomal and plasmid control is presented. Serological evidence that these molecular species of toxins are distinct from each other is given. The molecular weights of SHET from plasmidless...

journal_title：Journal of bacteriology

authors： Sato H,Watanabe T,Higuchi K,Teruya K,Ohtake A,Murata Y,Saito H,Aizawa C,Danbara H,Maehara N

更新日期：2000-07-01 00:00:00

abstract：:The nif promoters of Klebsiella pneumoniae must be activated by proteins bound to upstream sequences which are thought to interact with the sigma54-RNA polymerase holoenzyme by DNA looping. NifA is the activator for most of the promoters, and integration host factor (IHF) mediates the DNA looping. While NtrC is the ac...

journal_title：Journal of bacteriology

authors： Cheema AK,Choudhury NR,Das HK

更新日期：1999-09-01 00:00:00

abstract：:Kinsky, Stephen C. (Washington University, St. Louis, Mo.). Induction and repression of nitrate reductase in Neurospora crassa. J. Bacteriol. 82:898-904. 1961.-Techniques are described for studying induced enzyme formation in Neurospora crassa. The effects of various parameters (time, pH, nitrate concentration, etc.) ...

journal_title：Journal of bacteriology

authors： KINSKY SC

更新日期：1961-12-01 00:00:00

abstract：:In Escherichia coli, repression of the aceBAK operon is mediated by the IclR protein. We used an in vitro oligonucleotide selection technique to determine the consensus recognition sequence for MR. Mutational analysis confirmed the contribution of this sequence to repression in vivo and identified the -35 element of t...

journal_title：Journal of bacteriology

authors： Pan B,Unnikrishnan I,LaPorte DC

更新日期：1996-07-01 00:00:00

abstract：:Diploid Saccharomyces cerevisiae cells induce YVH1 expression and enter the developmental pathway, leading to sporulation when starved for nitrogen. We show that yvh1 disruption causes a defect in spore maturation; overexpression of MCK1 or IME1 suppresses this yvh1 phenotype. While mck1 mutations are epistatic to tho...

journal_title：Journal of bacteriology

authors： Beeser AE,Cooper TG

更新日期：1999-09-01 00:00:00

abstract：:Oligoribonuclease, an exoribonuclease specific for small oligoribonucleotides, was initially characterized 20 years ago (S. K. Niyogi and A. K. Datta, J. Biol. Chem. 250:7307-7312, 1975) and shown to be different from RNase II and polynucleotide phosphorylase. Here we demonstrate, using mutant strains and purified enz...

journal_title：Journal of bacteriology

authors： Yu D,Deutscher MP

更新日期：1995-07-01 00:00:00

abstract：:Biofilm formation is a key factor in Vibrio cholerae environmental survival and host colonization. Production of biofilm enables V. cholerae to survive and persist in aquatic environments and aids in the passage through the gastric acid barrier to allow access to the small intestine. The genes involved in biofilm form...

journal_title：Journal of bacteriology

authors： He H,Cooper JN,Mishra A,Raskin DM

更新日期：2012-06-01 00:00:00

abstract：:The FemAB-like factors Lif and Epr confer resistance to glycylglycine endopeptidases lysostaphin and Ale-1, respectively, by incorporating serine residues into the staphylococcal peptidoglycan interpeptide bridges specifically at positions 3 and 5. This required the presence of FemA and/or FemB, in contrast to earlier...

journal_title：Journal of bacteriology

authors： Ehlert K,Tschierske M,Mori C,Schröder W,Berger-Bächi B

更新日期：2000-05-01 00:00:00

abstract：:The structures of the lipooligosaccharides (LOSs) from Haemophilus ducreyi ITM 5535 and ITM 3147 and a fresh clinical isolate, ACY1, have been investigated. Oligosaccharides were obtained from phenol-water-extracted LOS by mild acid hydrolysis and were studied by methylation analysis, fast atom bombardment and electro...

journal_title：Journal of bacteriology

authors： Schweda EK,Jonasson JA,Jansson PE

更新日期：1995-09-01 00:00:00