Genetic analysis of Staphylococcus aureus RNA polymerase mutants.

abstract：:Phosphorylation of a major 57-kilodalton protein substrate was observed in cell lysates of Spiroplasma melliferum BC3 incubated with [gamma-32P]ATP. Only serine phosphates have been isolated from the acid hydrolysate of the phosphorylated protein. The 57-kilodalton protein substrate was found, to a large extent, in th...

journal_title：Journal of bacteriology

authors： Platt MW,Reizer J,Rottem S

更新日期：1990-05-01 00:00:00

abstract：:Late log-phase Escherichia coli B/r cells are 1.6 times more sensitive to killing by X rays than are stationary-phase cells when grown in Brain Heart Infusion (BHI) + glucose. The number of single-chain breaks formed per krad is the same for log- and stationary-phase cells. Stationary-phase cells show a somewhat great...

journal_title：Journal of bacteriology

authors： Town CD,Smith KC,Kaplan HS

更新日期：1971-01-01 00:00:00

abstract：:Mycoplasma pneumoniae cytadherence is mediated by a specialized, polar attachment organelle. Certain spontaneously arising cytadherence mutants (designated class I) lack HMW2, fail to localize the adhesin protein P1 to the attachment organelle, and exhibit accelerated turnover of proteins HMW1, HMW3, and P65. Insertio...

journal_title：Journal of bacteriology

authors： Fisseha M,Göhlmann HW,Herrmann R,Krause DC

更新日期：1999-07-01 00:00:00

abstract：:High-density tiling microarray and RNA sequencing technologies were used to analyze the transcriptome of the periodontopathogenic bacterium Porphyromonas gingivalis. The compiled P. gingivalis transcriptome profiles were based on total RNA samples isolated from three different laboratory culturing conditions, and the ...

journal_title：Journal of bacteriology

authors： Høvik H,Yu WH,Olsen I,Chen T

更新日期：2012-01-01 00:00:00

abstract：:Two nifA-like genes, designated anfA and vnfA, have been identified in Azotobacter vinelandii. The anfA gene is located upstream from the nitrogenase-3 structural gene cluster (anfHDGK) and is preceded by a sequence that is potentially part of a ntrA-dependent promoter. The product of anfA appears to be required for e...

journal_title：Journal of bacteriology

authors： Joerger RD,Jacobson MR,Bishop PE

更新日期：1989-06-01 00:00:00

abstract：:Inhibition of deoxyribonucleic acid (DNA) synthesis in Escherichia coli by chemical inhibitors or by shifting cultures of temperature-sensitive elongation (dnaE and dnaB) or initiation (dnaA) mutants to nonpermissive conditions led to greatly increased synthesis of the enzyme ribonucleoside diphosphate reductase, whic...

journal_title：Journal of bacteriology

authors： Filpula D,Fuchs JA

更新日期：1977-04-01 00:00:00

abstract：:The virulence of Staphylococcus aureus is controlled by the accessory gene regulator (agr) system, including an extracellular inducer encoded by agrD. Variable agr PCR restriction fragment length polymorphism (RFLP) patterns of unique S. aureus strains (n = 192) were determined for a region comprising agrD and parts o...

journal_title：Journal of bacteriology

authors： van Leeuwen W,van Nieuwenhuizen W,Gijzen C,Verbrugh H,van Belkum A

更新日期：2000-10-01 00:00:00

abstract：:The importance of the physiological state of a culture of Saccharomyces cerevisiae for tolerance to sudden osmotic dehydration was studied, and it was investigated whether specific osmotolerance factors were demonstrable. The microcalorimeter was used to monitor growth, and different physiological states of the cultur...

journal_title：Journal of bacteriology

authors： Blomberg A,Larsson C,Gustafsson L

更新日期：1988-10-01 00:00:00

abstract：:The gut microbiota plays a crucial role in the development of the immune system and confers benefits or disease susceptibility to the host. Emerging studies have indicated the gut microbiota could affect pulmonary health and disease through cross talk between the gut microbiota and the lungs. Gut microbiota dysbiosis c...

journal_title：Journal of bacteriology

authors： Zhou A,Lei Y,Tang L,Hu S,Yang M,Wu L,Yang S,Tang B

更新日期：2021-01-25 00:00:00

abstract：:Analysis of polypeptides in the crystalline delta-endotoxins from different Bacillus thuringiensis strains revealed two antigenically similar forms of the P2 protein which differed in molecular mass, peptide profile, and amino acid sequence. Purified preparations of the two forms displayed the characteristic dual toxi...

journal_title：Journal of bacteriology

authors： Nicholls CN,Ahmad W,Ellar DJ

更新日期：1989-09-01 00:00:00

abstract：:The lemA gene of Pseudomonas syringae pv. syringae encodes the sensor kinase of a bacterial two-component signal transduction system. Phenotypes that are lemA dependent in P. syringae include lesion formation on bean and production of extracellular protease and the antibiotic syringomycin. Recently, the gacA gene has ...

journal_title：Journal of bacteriology

authors： Kitten T,Willis DK

更新日期：1996-03-01 00:00:00

abstract：:Deoxyribonucleic acid repair synthesis was studied in one wild-type and two mutant strains of Bacillus subtilis that are defective in excision of pyrimidine dimers. The cells were irradiated with ultraviolet light, and 6-(p-hydroxyphenyl-azo)-uracil was used to block replicative synthesis, allowing only repair synthes...

journal_title：Journal of bacteriology

authors： Hadden CT

更新日期：1979-07-01 00:00:00

abstract：:The soluble hydrogenase of Desulfovibrio vulgaris was purified and some of its properties are described. The molecular weight was determined for the enzyme by sedimentation equilibrium (45,400) and amino acid analysis (44,800). The hydrogenase appears to be a loosely coiled molecule or to have a high axial ratio, whic...

journal_title：Journal of bacteriology

authors： Haschke RH,Campbell LL

更新日期：1971-01-01 00:00:00

abstract：:By means of the general procedure of Casadaban (J. Mol. Biol. 104: 541-556, 1976), the lac genes carried on a lambda-Mu-1 hybrid phage were inserted into a temperature-inducible Mu-1 prophage that had earlier been inserted into a site near the beginning of the ilvC gene of Escherichia coli strain K-12. Selection of te...

journal_title：Journal of bacteriology

authors： Smith JM,Umbarger HE

更新日期：1977-12-01 00:00:00

abstract：:NBU1 is an integrated Bacteroides element that can he mobilized from Bacteroides donors to Bacteroides recipients. Previous studies have shown that a plasmid carrying the internal mobilization region of NBU1 could be transferred by conjugation from Bacteroides thetaiotaomicron to Escherichia coli. In this report, we s...

journal_title：Journal of bacteriology

authors： Shoemaker NB,Wang GR,Salyers AA

更新日期：1996-06-01 00:00:00

abstract：:A procedure for the isolation of Bacillus subtilis mutants that appear to be defective in septum synthesis has been described. Fourteen mutants isolated with this technique were found to be located at four distinct loci on the B. subtilis chromosome. These have been designated divA, divB, divC, and divD. The four muta...

journal_title：Journal of bacteriology

authors： Van Alstyne D,Simon MI

更新日期：1971-12-01 00:00:00

abstract：:Transfection of streptococci is reported here for the first time. ...

journal_title：Journal of bacteriology

authors： Parsons CL,Cole RM

更新日期：1973-03-01 00:00:00

abstract：:The central metabolic model for Geobacter sulfurreducens included a single pathway for the biosynthesis of isoleucine that was analogous to that of Escherichia coli, in which the isoleucine precursor 2-oxobutanoate is generated from threonine. 13C labeling studies performed in G. sulfurreducens indicated that this pat...

journal_title：Journal of bacteriology

authors： Risso C,Van Dien SJ,Orloff A,Lovley DR,Coppi MV

更新日期：2008-04-01 00:00:00

abstract：:The deacetylcephalosporin C synthetase (hydroxylase) gene from Streptomyces clavuligerus has been cloned and sequenced. The open reading frame codes for a protein with an Mr of 34,584. The hydroxylase gene (cefF) is closely linked to the epimerase gene (cefD) and the expandase gene (cefE) and is transcribed in the opp...

journal_title：Journal of bacteriology

authors： Kovacevic S,Miller JR

更新日期：1991-01-01 00:00:00

abstract：:The lactose-H+ symport protein (LacS) of Streptococcus thermophilus has a carboxyl-terminal regulatory domain (IIALacS) that is homologous to a family of proteins and protein domains of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) in various organisms, of which IIAGlc of Escherichia coli is the...

journal_title：Journal of bacteriology

authors： Gunnewijk MG,Postma PW,Poolman B

更新日期：1999-01-01 00:00:00

abstract：:Deletions of the uvrB gene were not obtained in polA1 strains of Escherichia coli either by selecting for spontaneous deletions or by transduction from strains carrying such deletions. A strain forming a temperature-sensitive deoxyribonucleic acid polymerase I and carrying a deletion of the uvrB gene is inviable at th...

journal_title：Journal of bacteriology

authors： Shizuya H,Dykhuizen D

更新日期：1972-11-01 00:00:00

abstract：:At early stages of sporulation, the spoIIA locus is transcribed as a tricistronic (1.7-kb) operon, coding for sigma F and for two proteins that modulate the activity of sigma F. The locus is transcribed as a longer (2.9-kb) transcript at the late stages of sporulation. We show here that the longer transcript contains ...

journal_title：Journal of bacteriology

authors： Wu JJ,Schuch R,Piggot PJ

更新日期：1992-08-01 00:00:00

abstract：:Tectiviridae is a family of tailless bacteriophages with Gram-negative and Gram-positive hosts. The family model PRD1 and its close relatives all infect a broad range of enterobacteria by recognizing a plasmid-encoded conjugal transfer complex as a receptor. In contrast, tectiviruses with Gram-positive hosts are highl...

journal_title：Journal of bacteriology

authors： Kan S,Fornelos N,Schuch R,Fischetti VA

更新日期：2013-10-01 00:00:00

abstract：:Burkholderia pseudomallei, the causative agent of melioidosis, contains a large pathogen genome (7.2 Mb) with ∼2,000 genes of putative or unknown function. Interactions with potential hosts and environmental factors may induce rapid adaptations in these B. pseudomallei genes, which can be discerned through evolutionar...

journal_title：Journal of bacteriology

authors： Singh AP,Lai SC,Nandi T,Chua HH,Ooi WF,Ong C,Boyce JD,Adler B,Devenish RJ,Tan P

更新日期：2013-12-01 00:00:00

abstract：:An electron microscopy study has been made of the effects of dissolution of the plasma membrane of Escherichia coli with sodium dodecyl sulfate (SDS) on the organization of the nucleoplasm and the cytoplasm. The alterations observed in time course experiments were related to absorbance changes and to release of macrom...

journal_title：Journal of bacteriology

authors： Woldringh CL,van Iterson W

更新日期：1972-09-01 00:00:00

abstract：:A DNA fragment from Xanthomonas campestris pv. campestris that partially restored the carbohydrate fermentation pattern of a cya crp Escherichia coli strain was cloned and expressed in E. coli. The nucleotide sequence of this fragment revealed the presence of a 700-base-pair open reading frame that coded for a protein...

journal_title：Journal of bacteriology

authors： de Crecy-Lagard V,Glaser P,Lejeune P,Sismeiro O,Barber CE,Daniels MJ,Danchin A

更新日期：1990-10-01 00:00:00

abstract：:Two isoleucine analogues and two leucine analogues were examined for their ability to replace the natural amino acid preventing the accumulation of threonine deaminase-forming potential. The procedure used to study repression by the analogues distinguishes between true repression and the formation of inactive enzyme b...

journal_title：Journal of bacteriology

authors： Wasmuth JJ,Umbarger HE

更新日期：1973-11-01 00:00:00

abstract：:The gene encoding a 45-kDa protein (45K) of Chlamydia trachomatis serovar F was cloned, sequenced, and overexpressed in Escherichia coli. Alignment of the deduced peptide sequence with E. coli elongation factor Tu (EF-Tu) demonstrated 69% identity. The 45K was recognized by a Chlamydia genus-specific monoclonal antibo...

journal_title：Journal of bacteriology

authors： Zhang YX,Shi Y,Zhou M,Petsko GA

更新日期：1994-02-01 00:00:00

abstract：:Bacillus subtilis possesses different enzymes for the utilization of plant cell wall polysaccharides. This includes a gene cluster containing galactan degradation genes (ganA and ganB), two transporter component genes (ganQ and ganP), and the sugar-binding lipoprotein-encoding gene ganS (previously known as cycB). The...

journal_title：Journal of bacteriology

authors： Watzlawick H,Morabbi Heravi K,Altenbuchner J

更新日期：2016-09-22 00:00:00

abstract：:Enzymological studies were done to characterize the allosteric control of 3-deoxy-d-arabino-heptulosonate 7-phosphate (DAHP) synthetase in three species of Clostridium. Allosteric control was identified as feedback inhibition by phenylalanine and was qualitatively similar for the DAHP synthetases of C. butyricum, C. a...

journal_title：Journal of bacteriology

authors： Jensen RA,Twarog R

更新日期：1972-09-01 00:00:00