Gap-filling repair synthesis induced by ultraviolet light in a Bacillus subtilis Uvr- mutant.

Abstract:

:Deoxyribonucleic acid repair synthesis was studied in one wild-type and two mutant strains of Bacillus subtilis that are defective in excision of pyrimidine dimers. The cells were irradiated with ultraviolet light, and 6-(p-hydroxyphenyl-azo)-uracil was used to block replicative synthesis, allowing only repair synthesis. One of the mutations (uvs-42) resulted in a severe inhibition of incision, dimer excision, and repair synthesis. In contrast, the other mutant (uvr-1) slowly incised and excised dimers and did repair synthesis in patches which appear to be several-fold longer than those in the wild-type strain, apparently because large gaps are produced at excision sites. The results indicate that the primary defect in uvs-42 cells is in initiation of dimer excision, whereas the uvr-1 mutation appears to be a defect in the exonuclease normally used to complete dimer excision.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Hadden CT

doi

10.1128/JB.139.1.239-246.1979

keywords:

subject

Has Abstract

pub_date

1979-07-01 00:00:00

pages

239-46

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

139

pub_type

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