Interchangeability and specificity of components from the quorum-sensing regulatory systems of Vibrio fischeri and Pseudomonas aeruginosa.

Abstract:

:Autoinduction is a conserved mechanism of cell density-dependent gene regulation that occurs in a variety of gram-negative bacteria. Autoinducible luminescence in Vibrio fischeri requires a transcriptional activator, LuxR, while a LuxR homolog, LasR, activates elastase expression in Pseudomonas aeruginosa. Both LuxR and LasR require specific signal molecules, called autoinducers, for activity. We show here the activation in Escherichia coli of the V. fischeri luminescence (lux) operon by LasR and of the P. aeruginosa elastase gene (lasB) by LuxR when each is in the presence of its cognate autoinducer. Neither LuxR nor LasR showed appreciable activity with the heterologous V. fischeri or P. aeruginosa autoinducer. This supports the view that there is a direct interaction of each transcriptional activator with its proper autoinducer and suggests that there are conserved, autoinduction-related elements within the promoter regions of these genes.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Gray KM,Passador L,Iglewski BH,Greenberg EP

doi

10.1128/jb.176.10.3076-3080.1994

subject

Has Abstract

pub_date

1994-05-01 00:00:00

pages

3076-80

issue

10

eissn

0021-9193

issn

1098-5530

journal_volume

176

pub_type

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