Cloning and nucleotide sequence of the type E staphylococcal enterotoxin gene.

abstract：:Transcriptional regulation of the Mg(2+)-citrate transporter, CitM, the main citrate uptake system of Bacillus subtilis, was studied during growth in rich medium. Citrate in the growth medium was required for induction under all growth conditions. In Luria-Bertani medium containing citrate, citM expression was complet...

journal_title：Journal of bacteriology

authors： Warner JB,Magni C,Lolkema JS

更新日期：2003-02-01 00:00:00

abstract：:The distribution of a pulse of teichoic acid-specific radiolabel between wall and membrane teichoic acids in pneumococci was constant over a subsequent chase period, suggesting that wall and membrane teichoic acids are biosynthesized simultaneously and independently. ...

journal_title：Journal of bacteriology

authors： Briles EB,Tomasz A

更新日期：1975-04-01 00:00:00

abstract：:A fusion between lacZ and ftsZ of Escherichia coli was constructed to obtain a beta-galactosidase-FtsZ fusion protein. This fusion protein was used to raise antibodies against cell division protein FtsZ. Six monoclonal antibodies were obtained, and they reacted with FtsZ from cytoplasm and membrane fractions. The epit...

journal_title：Journal of bacteriology

authors： Voskuil JL,Westerbeek CA,Wu C,Kolk AH,Nanninga N

更新日期：1994-04-01 00:00:00

abstract：:Fine details of the internal and external morphology of the yeastlike and mycelial phases of the dimorphic fungal pathogen Sporotrichum schenckii as seen in ultrathin sections are described and illustrated by electronphotomicrography. Comparisons of yeastlike phase ultrastructure were made using two different methods ...

journal_title：Journal of bacteriology

authors： Lane JW,Grrison RG,Field MF

更新日期：1969-11-01 00:00:00

abstract：:We previously developed a genetic approach to study, with a single antibody, the topology of the outer membrane protein LamB, an Escherichia coli porin with specificity towards maltodextrins and a receptor for bacteriophage lambda. Our initial procedure consisted of inserting at random the same reporter epitope (the C...

journal_title：Journal of bacteriology

authors： Newton SM,Klebba PE,Michel V,Hofnung M,Charbit A

更新日期：1996-06-01 00:00:00

abstract：:Escherichia coli outer membranes were prepared by centrifugation to equilibrium in sucrose gradients and then treated with Sarkosyl in the presence of ethylenediaminetetraacetate. The polypeptide profiles of the two outer membrane preparations were compared by two-dimensional polyacrylamide gel electrophoresis. The pa...

journal_title：Journal of bacteriology

authors： Chopra I,Shales SW

更新日期：1980-10-01 00:00:00

abstract：:During the progression of Mycoplasma hominis cultures from the early logarithmic phase to the stationary phase of growth, the total phospholipid content of the cell membranes decreased. Measurement of the amount of the various phospholipids during the growth cycle showed that a decrease in the phosphatidylglycerol (PG...

journal_title：Journal of bacteriology

authors： Rottem S,Greenberg AS

更新日期：1975-02-01 00:00:00

abstract：:Mycoplasma pneumoniae adherence to host cells is a multifactorial process that requires the cytadhesin P1 and additional accessory proteins. The hmw gene cluster consists of the genes p30, hmw3, and hmw1, the products of which are known to be essential for cytadherence, the rpsD gene, and six open reading frames of un...

journal_title：Journal of bacteriology

authors： Waldo RH 3rd,Popham PL,Romero-Arroyo CE,Mothershed EA,Lee KK,Krause DC

更新日期：1999-08-01 00:00:00

abstract：:The exosporium of Bacillus anthracis is comprised of two distinct layers: a basal layer and a hair-like nap that covers the basal layer. The hair-like nap contains the glycoproteins BclA and, most likely, BclB. BclA and BclB are directed to assemble into the exosporium by motifs in their N-terminal domains. Here, we i...

journal_title：Journal of bacteriology

authors： Thompson BM,Hoelscher BC,Driks A,Stewart GC

更新日期：2011-10-01 00:00:00

abstract：:Our previous work showed that pap pilin gene transcription is subject to a thermoregulatory control mechanism under which pap pilin is not transcribed at a low temperature (23 degrees C) (L. B. Blyn, B. A. Braaten, C. A. White-Ziegler, D. H. Rolfson, and D. A. Low, EMBO J. 8:613-620, 1989). In order to isolate genes i...

journal_title：Journal of bacteriology

authors： White-Ziegler CA,Low DA

更新日期：1992-11-01 00:00:00

abstract：:Biogenesis of the outer membrane (OM) is an essential process in gram-negative bacteria. One of the key steps of OM biogenesis is the assembly of integral outer membrane beta-barrel proteins (OMPs) by a protein machine called the Bam complex. In Escherichia coli, the Bam complex is composed of the essential proteins B...

journal_title：Journal of bacteriology

authors： Rigel NW,Schwalm J,Ricci DP,Silhavy TJ

更新日期：2012-03-01 00:00:00

abstract：:Time-lapse imaging of Streptomyces hyphae revealed foci of the essential protein DivIVA at sites where lateral branches will emerge. Overexpression experiments showed that DivIVA foci can trigger establishment of new zones of cell wall assembly, suggesting a key role of DivIVA in directing peptidoglycan synthesis and ...

journal_title：Journal of bacteriology

authors： Hempel AM,Wang SB,Letek M,Gil JA,Flärdh K

更新日期：2008-11-01 00:00:00

abstract：:Methods were devised to purify the cell-associated, iron-binding compounds known as mycobactins from the closely related species Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum (i.e., the MAIS complex of organisms). The mycobactins from these three species showed a structure that is c...

journal_title：Journal of bacteriology

authors： Barclay R,Ewing DF,Ratledge C

更新日期：1985-11-01 00:00:00

abstract：:Pentaerythritol tetranitrate reductase, which reductively liberates nitrite from nitrate esters, is related to old yellow enzyme. Pentaerythritol tetranitrate reductase follows a ping-pong mechanism with competitive substrate inhibition by NADPH, is strongly inhibited by steroids, and is capable of reducing the unsatu...

journal_title：Journal of bacteriology

authors： French CE,Nicklin S,Bruce NC

更新日期：1996-11-01 00:00:00

abstract：:The pckA gene of Rhizobium meliloti, encoding phosphoenolpyruvate carboxykinase, was isolated from a genomic cosmid library by complementation of the succinate growth phenotype of a Pck- mutant. The gene region was mapped by subcloning and Tn5 insertion mutagenesis. The DNA sequence for a 2-kb region containing the st...

journal_title：Journal of bacteriology

authors： Osterås M,Driscoll BT,Finan TM

更新日期：1995-03-01 00:00:00

abstract：:A partial nucleotide sequence that included 1,693 base pairs of the M12 (emm12) gene of group A streptococci (strain CS24) and adjacent upstream DNA was determined. Type 12 M protein-specific mRNA of strain CS24 is transcribed from two promoters (P1 and P3) separated by 30 bases. The transcription start sites of the e...

journal_title：Journal of bacteriology

authors： Robbins JC,Spanier JG,Jones SJ,Simpson WJ,Cleary PP

更新日期：1987-12-01 00:00:00

abstract：:Twelve mutants of Escherichia coli K-12 have been isolated which carry chromosomal mutations that exhibit pleiotropic effects on the expression of F factor tra cistrons. F pilus synthesis, deoxyribonucleic acid transfer, and surface exclusion are all inhibited. Six of the mutants carry sfrA mutations, and six carry sf...

journal_title：Journal of bacteriology

authors： Beutin L,Achtman M

更新日期：1979-09-01 00:00:00

abstract：:A survey of chromosomal variation in the ST239 clonal group of methicillin-resistant Staphylococcus aureus (MRSA) revealed a novel genetic element, ICE6013. The element is 13,354 bp in length, excluding a 6,551-bp Tn552 insertion. ICE6013 is flanked by 3-bp direct repeats and is demarcated by 8-bp imperfect inverted r...

journal_title：Journal of bacteriology

authors： Smyth DS,Robinson DA

更新日期：2009-10-01 00:00:00

abstract：:We have cloned and sequenced a cluster of six open reading frames containing gene kdsA from Escherichia coli K-12. The gene encodes 3-deoxy-D-manno-octulosonate 8-phosphate synthetase (KDO-8-phosphate synthetase), which catalyzes formation of 3-deoxy-D-manno-octulosonic acid (KDO), an essential component of enterobact...

journal_title：Journal of bacteriology

authors： Strohmaier H,Remler P,Renner W,Högenauer G

更新日期：1995-08-01 00:00:00

abstract：:The rates of thermal denaturation and the molecular weights of the two isofunctional enol-lactone hydrolases (ELH I and ELH II) of Acinetobacter calcoaceticus were determined. The molecular weights of ELH I and ELH II were found by gel filtration to be approximately 24,000 and 21,000, respectively. In crude extract at...

journal_title：Journal of bacteriology

authors： Katagiri M,Wheelis ML

更新日期：1971-05-01 00:00:00

abstract：:Some Escherichia coli strains with impaired cell division form branched cells at high frequencies during certain growth conditions. Here, we show that neither FtsI nor FtsZ activity is required for the development of branches. Buds did not form at specific positions along the cell surface during high-branching conditi...

journal_title：Journal of bacteriology

authors： Gullbrand B,Akerlund T,Nordström K

更新日期：1999-11-01 00:00:00

abstract：:In Bacillus thuringiensis, a novel N-acetylmuramoyl-L-alanine amidase gene (named cwlB) was detected, and the CwlB protein was purified and characterized. Reverse transcription-PCR (RT-PCR) results indicated that cwlB and an upstream gene (named cwlA) formed one transcriptional unit. 5' rapid amplification of cDNA end...

journal_title：Journal of bacteriology

authors： Yang J,Peng Q,Chen Z,Deng C,Shu C,Zhang J,Huang D,Song F

更新日期：2013-06-01 00:00:00

abstract：:Enterococcus faecalis is equipped with two pathways of glycerol dissimilation. Glycerol can either first be phosphorylated by glycerol kinase and then oxidized by glycerol-3-phosphate oxidase (the glpK pathway) or first be oxidized by glycerol dehydrogenase and then phosphorylated by dihydroxyacetone kinase (the dhaK ...

journal_title：Journal of bacteriology

authors： Bizzini A,Zhao C,Budin-Verneuil A,Sauvageot N,Giard JC,Auffray Y,Hartke A

更新日期：2010-02-01 00:00:00

abstract：:The DNA sequence of the gene which codes for the major outer membrane porin (Omp32) of Comamonas acidovorans has been determined. The structural gene encodes a precursor consisting of 351 amino acid residues with a signal peptide of 19 amino acid residues. Comparisons with amino acid sequences of outer membrane protei...

journal_title：Journal of bacteriology

authors： Gerbl-Rieger S,Peters J,Kellermann J,Lottspeich F,Baumeister W

更新日期：1991-04-01 00:00:00

abstract：:A 10-kb region of the Bacillus subtilis genome that contains genes involved in biotin-biosynthesis was cloned and sequenced. DNA sequence analysis indicated that B. subtilis contains homologs of the Escherichia coli and Bacillus sphaericus bioA, bioB, bioD, and bioF genes. These four genes and a homolog of the B. spha...

journal_title：Journal of bacteriology

authors： Bower S,Perkins JB,Yocum RR,Howitt CL,Rahaim P,Pero J

更新日期：1996-07-01 00:00:00

abstract：:The penultimate step in the biosynthesis of riboflavin (vitamin B2) involves the condensation of 3,4-dihydroxy-2-butanone 4-phosphate with 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione, which is catalyzed by 6,7-dimethyl-8-ribityllumazine synthase (lumazine synthase). Pathogenic Brucella species adapted to an intr...

journal_title：Journal of bacteriology

authors： Zylberman V,Klinke S,Haase I,Bacher A,Fischer M,Goldbaum FA

更新日期：2006-09-01 00:00:00

abstract：:Repaske, Roy (National Institute of Allergy and Infectious Diseases, Bethesda, Md.). Nutritional requirements for Hydrogenomonas eutropha. J. Bacteriol. 83: 418-422. 1962.-A simple apparatus for the autotrophic cultivation of Hydrogenomonas eutropha in 100-ml shake cultures is described. Nitrogen, in the form of ammon...

journal_title：Journal of bacteriology

authors： REPASKE R

更新日期：1962-02-01 00:00:00

abstract：:During growth of the halophilic archaeon Haloarcula marismortui on D-xylose, a specific D-xylose dehydrogenase was induced. The enzyme was purified to homogeneity. It constitutes a homotetramer of about 175 kDa and catalyzed the oxidation of xylose with both NADP+ and NAD+ as cosubstrates with 10-fold higher affinity ...

journal_title：Journal of bacteriology

authors： Johnsen U,Schönheit P

更新日期：2004-09-01 00:00:00

abstract：:The nucleotide sequence of the DNA encoding the ADP-ribosyltransferase (A1) fragment of cholera enterotoxin was determined. A putative precursor of the A1 peptide contains an 18-amino acid leader peptide, and the mature A1 peptide contains 194 amino acids. The primary structure of the A1 fragment from cholera enteroto...

journal_title：Journal of bacteriology

authors： Lockman HA,Galen JE,Kaper JB

更新日期：1984-09-01 00:00:00

abstract：:Thiamine pyrophosphate (TPP), the biologically active form of thiamine (also known as vitamin B1), is an essential cofactor for several important enzymes involved in carbohydrate metabolism, and therefore, it is required for all living organisms. We recently found that a thiamine-binding protein (TDE_0143) is essentia...

journal_title：Journal of bacteriology

authors： Bian J,Tu Y,Wang SM,Wang XY,Li C

更新日期：2015-04-01 00:00:00