Characterization of a Bacillus subtilis sporulation operon that includes genes for an RNA polymerase sigma factor and for a putative DD-carboxypeptidase.

Abstract:

:At early stages of sporulation, the spoIIA locus is transcribed as a tricistronic (1.7-kb) operon, coding for sigma F and for two proteins that modulate the activity of sigma F. The locus is transcribed as a longer (2.9-kb) transcript at the late stages of sporulation. We show here that the longer transcript contains an additional open reading frame whose product has extensive sequence homology with DD-carboxypeptidases; the corresponding gene is designated dacF. Cotranscription of a morphogene, such as dacF, with the gene for a sigma factor suggests a way to couple transcription regulation with morphogenesis. The predicted N-terminal sequence of the DacF protein and the inhibition of sporulation by a translational dacF-lacZ fusion both suggest that the protein has a signal peptide for transport into or across a membrane. Expression of a dacF-lacZ transcriptional fusion was in the forespore. The 5' end of the 2.9-kb transcript was determined by primer extension analysis. The region 5' to the end showed no homology to promoters recognized by known sigma factors but was homologous to the corresponding region of the forespore-specific 0.3-kb gene of Bacillus subtilis.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Wu JJ,Schuch R,Piggot PJ

doi

10.1128/jb.174.15.4885-4892.1992

keywords:

subject

Has Abstract

pub_date

1992-08-01 00:00:00

pages

4885-92

issue

15

eissn

0021-9193

issn

1098-5530

journal_volume

174

pub_type

杂志文章
  • Immunochemistry of the pneumococcal types II, V, and VI. III. Tests with derivatives of the specific polysaccharides of types II and VI.

    abstract::Rebers, P. A. (Rutgers, The State University, New Brunswick, N. J.), E. Hurwitz, M. Heidelberger, and S. Estrada-Parra. Immunochemistry of the pneumococcal types II, V, and VI. III. Tests with derivatives of the specific polysaccharides of types II and VI. J. Bacteriol. 83:335-342. 1962.-The type-specific pneumococcal...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.2.335-342.1962

    authors: REBERS PA,HURWITZ E,HEIDELBERGER M,ESTRADA-PARRA S

    更新日期:1962-02-01 00:00:00

  • Plasmid formation after lambda bacteriophage infection of Escherichia coli-Salmonella typhosa hybrids.

    abstract::Defective phage lambdadg, when present in certain Salmonella typhosa hybrids, could be eliminated with acridine orange or ethidium bromide treatment. The lambdadg deoxyribonucleic acid could be separated from the S. typhosa host deoxyribonucleic acid as a distinctly covalently closed molecule. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.102.1.288-290.1970

    authors: Falkow S,Baron LS

    更新日期:1970-04-01 00:00:00

  • Mycobacterium tuberculosis Latin American-Mediterranean family and its sublineages in the light of robust evolutionary markers.

    abstract::Mycobacterium tuberculosis has a clonal population structure, and the Latin American-Mediterranean (LAM) family is one of the largest and most widespread within this species, showing evidence for remarkable pathobiology and a confusing phylogeny. Here, we applied robust phylogenetic markers to study the evolution of t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01485-13

    authors: Mokrousov I,Vyazovaya A,Narvskaya O

    更新日期:2014-05-01 00:00:00

  • The serotype of type Ia and III group B streptococci is determined by the polymerase gene within the polycistronic capsule operon.

    abstract::Streptococcus agalactiae is a primary cause of neonatal morbidity and mortality. Essential to the virulence of this pathogen is the production of a type-specific capsular polysaccharide (CPS) that enables the bacteria to evade host immune defenses. The identification, cloning, sequencing, and functional characterizati...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.16.4466-4477.2000

    authors: Chaffin DO,Beres SB,Yim HH,Rubens CE

    更新日期:2000-08-01 00:00:00

  • Effect of Selenomethionine on Growth of Escherichia coli and Bacillus megaterium.

    abstract::Studies with methionineless strains of Escherichia coli WWU and Bacillus megaterium KM have shown that selenomethionine only partially satisfies their methionine requirement. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.104.3.1393-1396.1970

    authors: Wu M,Wachsman JT

    更新日期:1970-12-01 00:00:00

  • Random replication of the stringent plasmid R1 in Escherichia coli K-12.

    abstract::The R-factor R1 is present in a low number of copies per genome (near unity, so-called stringent control of replication). The replication of R1 was studied in a density-shift experiment. One generation after the shift about 20% of the R1 copies had not replicated, whereas about 20% had replicated at least twice. The r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.123.2.443-448.1975

    authors: Gustafsson P,Nordström K

    更新日期:1975-08-01 00:00:00

  • Characterization of an Escherichia coli aromatic hydroxylase with a broad substrate range.

    abstract::The hpaB gene encoding an aromatic hydroxylase of Escherichia coli ATCC 11105, a penicillin G acylase-producing strain, has been cloned and expressed in E. coli K-12. This gene was located near the pacA gene coding for penicillin G acylase. The hydroxylase has a molecular mass of 59,000 Da, uses NADH as a cosubstrate,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.7.2162-2167.1993

    authors: Prieto MA,Perez-Aranda A,Garcia JL

    更新日期:1993-04-01 00:00:00

  • Homologous c-di-GMP-Binding Scr Transcription Factors Orchestrate Biofilm Development in Vibrio parahaemolyticus.

    abstract::The marine bacterium and human pathogen Vibrio parahaemolyticus rapidly colonizes surfaces by using swarming motility and forming robust biofilms. Entering one of the two colonization programs, swarming motility or sessility, involves differential regulation of many genes, resulting in a dramatic shift in physiology a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00723-19

    authors: Kimbrough JH,Cribbs JT,McCarter LL

    更新日期:2020-02-25 00:00:00

  • Promoter-probe plasmid for Bacillus subtilis.

    abstract::We have constructed a promoter-probe expression vector for Bacillus subtilis. This plasmid, pCED6, can be used to fuse various DNA sequences to the structural gene of Escherichia coli beta-galactosidase, permitting analysis of the promoter activity of such sequences. pCED6 replicates and confers drug resistances in bo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.157.3.965-967.1984

    authors: Donnelly CE,Sonenshein AL

    更新日期:1984-03-01 00:00:00

  • Molecular cloning and analysis of genes for production of K5, K7, K12, and K92 capsular polysaccharides in Escherichia coli.

    abstract::With a DNA fragment from within the region encoding the transport functions for K1 production as a hybridization probe in Southern blot experiments, homologous DNA sequences were detected in the DNA from Escherichia coli strains producing K5, K7, K92, and K100 capsular polysaccharides. No homology with the laboratory ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.168.3.1228-1233.1986

    authors: Roberts I,Mountford R,High N,Bitter-Suermann D,Jann K,Timmis K,Boulnois G

    更新日期:1986-12-01 00:00:00

  • "Position effect" on dominance in the D-serine deaminase system of Escherichia coli K-12.

    abstract::A completely stable F32 merodiploid has been isolated, which has the dsd genotype dsdA(+)dsdCx3 /F -dsdA(+)dsdC(+). The stabilization apparently does not result from an alteration in the F, or from its integration in the chromosome, but rather from a chromosomal aberration. The stable strain can exist in two forms, wh...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.6.1989-1993.1967

    authors: McFall E

    更新日期:1967-12-01 00:00:00

  • Maleylacetate reductase of Pseudomonas sp. strain B13: specificity of substrate conversion and halide elimination.

    abstract::Maleylacetate reductase (EC 1.3.1.32) plays a major role in the degradation of chloroaromatic compounds by channelling maleylacetate and some chlorinated derivatives into the 3-oxoadipate pathway. Several substituted maleylacetates were prepared in situ by alkaline or enzymatic hydrolysis of dienelactones as the precu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.2.320-325.1995

    authors: Kaschabek SR,Reineke W

    更新日期:1995-01-01 00:00:00

  • Repression of cytosine deaminase by pyrimidines in Salmonella typhimurium.

    abstract::The synthesis of cytosine deaminase in Salmonella typhimurium is repressed by pyrimidines. This repression is mediated by both a uridine and a cytidine compound, indicating a distinct difference in the regulation of synthesis of cytosine deaminase from the regulation of the de novo pyrimidine pathway enzymes. A salvag...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.149.3.1171-1174.1982

    authors: West TP,O'Donovan GA

    更新日期:1982-03-01 00:00:00

  • Translation rates of individual codons are not correlated with tRNA abundances or with frequencies of utilization in Escherichia coli.

    abstract::We analyzed 12 individual codons, which differed widely with respect to the frequency of use in Escherichia coli and the abundance of the corresponding tRNAs, for their influence on the coupling between transcription and translation. This was probed by determining the effects of codon substitutions in the leader pepti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.11.5812-5816.1989

    authors: Bonekamp F,Dalbøge H,Christensen T,Jensen KF

    更新日期:1989-11-01 00:00:00

  • Albomycin uptake via a ferric hydroxamate transport system of Streptococcus pneumoniae R6.

    abstract::The antibiotic albomycin is highly effective against Streptococcus pneumoniae, with an MIC of 10 ng/ml. The reason for the high efficacy was studied by measuring the uptake of albomycin into S. pneumoniae. Albomycin was transported via the system that transports the ferric hydroxamates ferrichrome and ferrioxamine B. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00205-06

    authors: Pramanik A,Braun V

    更新日期:2006-06-01 00:00:00

  • Reproducible and rapid methods for the isolation and assay of a-factor, a yeast mating hormone.

    abstract::The Saccharomyces cerevisiae mating hormone a-factor has been difficult to isolate reproducibly in sufficient yields by methods using ion-exchange chromatography, probably because of its pronounced hydrophobicity. In this work, a hydrophobic adsorbent (Amberlite XAD-2), in an insoluble bead from, was used to isolate l...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.151.3.1153-1161.1982

    authors: Strazdis JR,MacKay VL

    更新日期:1982-09-01 00:00:00

  • Effects of carbon source and growth rate on cell wall composition of Bacillus subtilis subsp. niger.

    abstract::A study was made to determine whether factors other than the availability of phosphorus were involved in the regulation of synthesis of teichoic and teichuronic acids in Bacillus subtilis subsp. niger WM. First, the nature of the carbon source was varied while the dilution rate was maintained at about 0.3 h-1. Irrespe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.144.1.238-246.1980

    authors: Kruyssen FJ,de Boer WR,Wouters JT

    更新日期:1980-10-01 00:00:00

  • Secretory leukocyte protease inhibitor binding to mRNA and DNA as a possible cause of toxicity to Escherichia coli.

    abstract::The expression of the positively charged human protein secretory leukocyte protease inhibitor (SLPI) in Escherichia coli causes severe cellular toxicity. After induction of SLPI synthesis in a high-level-expression strain, SGE61, the growth of the strain is arrested and total protein and RNA synthesis rates decline by...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.4.2166-2172.1989

    authors: Miller KW,Evans RJ,Eisenberg SP,Thompson RC

    更新日期:1989-04-01 00:00:00

  • Estimations of bacterial growth rates in natural waters.

    abstract::Specific growth rates as low as 0.005 hr(-1) (generation times of 20 to 200 hr) of aquatic bacteria in natural waters have been calculated from significant differences between dilution rates and washout rates in a chemostat. The measured growth rates were affected by the treatment of the water samples (type of sterili...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.99.1.156-160.1969

    authors: Jannasch HW

    更新日期:1969-07-01 00:00:00

  • Structural analysis and biosynthesis gene cluster of an antigenic glycopeptidolipid from Mycobacterium intracellulare.

    abstract::Mycobacterium avium-Mycobacterium intracellulare complex (MAC) is the most common isolate of nontuberculous mycobacteria and causes pulmonary and extrapulmonary diseases. MAC species can be grouped into 31 serotypes by the epitopic oligosaccharide structure of the species-specific glycopeptidolipid (GPL) antigen. The ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01850-07

    authors: Fujiwara N,Nakata N,Naka T,Yano I,Doe M,Chatterjee D,McNeil M,Brennan PJ,Kobayashi K,Makino M,Matsumoto S,Ogura H,Maeda S

    更新日期:2008-05-01 00:00:00

  • Functional analysis of luxS in Staphylococcus aureus reveals a role in metabolism but not quorum sensing.

    abstract::The function of AI-2 in many bacteria and the physiological role of LuxS, the enzyme responsible for its production, remain matters of debate. Here, we show that in Staphylococcus aureus the luxS gene forms a monocistronic transcriptional unit under the control of a sigma(70)-dependent promoter. The gene was transcrib...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.8.2885-2897.2006

    authors: Doherty N,Holden MT,Qazi SN,Williams P,Winzer K

    更新日期:2006-04-01 00:00:00

  • Nucleotide sequences of the fecBCDE genes and locations of the proteins suggest a periplasmic-binding-protein-dependent transport mechanism for iron(III) dicitrate in Escherichia coli.

    abstract::The fec region of the Escherichia coli chromosome determines a citrate-dependent iron(III) transport system. The nucleotide sequence of fec revealed five genes, fecABCDE, which are transcribed from fecA to fecE. The fecA gene encodes a previously described outer membrane receptor protein. The fecB gene product is form...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.5.2626-2633.1989

    authors: Staudenmaier H,Van Hove B,Yaraghi Z,Braun V

    更新日期:1989-05-01 00:00:00

  • Overexpression of LolCDE allows deletion of the Escherichia coli gene encoding apolipoprotein N-acyltransferase.

    abstract::Bacterial lipoproteins represent a subset of membrane-associated proteins that are covalently modified with lipids at the N-terminal cysteine. The final step of lipoprotein modification, N-acylation of apolipoproteins, is mediated by apolipoprotein N-acyltransferase (Lnt). Examinations with reconstituted proteoliposom...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05013-11

    authors: Narita S,Tokuda H

    更新日期:2011-09-01 00:00:00

  • Cloning, characterization, and effects of overexpression of the Escherichia coli rnd gene encoding RNase D.

    abstract::RNase D is a 3'-exoribonuclease whose in vitro specificity has suggested that it is involved in the processing of tRNA precursors. Its in vivo role has remained unclear, however, because mutant cells devoid of the enzyme display no defect in growth or tRNA processing. To learn more about the structure and function of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.2.522-527.1988

    authors: Zhang JR,Deutscher MP

    更新日期:1988-02-01 00:00:00

  • ToxR proteins with substitutions in residues conserved with OmpR fail to activate transcription from the cholera toxin promoter.

    abstract::The ToxR protein of Vibrio cholerae is an integral membrane protein that coordinately regulates the expression of virulence genes required for successful infection. ToxR has been shown to bind directly to and activate transcription of the cholera toxin (ctx) promoter. Within the amino-terminal cytoplasmic region of To...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.21.6807-6814.1992

    authors: Ottemann KM,DiRita VJ,Mekalanos JJ

    更新日期:1992-11-01 00:00:00

  • Identification and characterization of a membrane permease involved in iron-hydroxamate transport in Staphylococcus aureus.

    abstract::Staphylococcus aureus was shown to transport iron complexed to a variety of hydroxamate type siderophores, including ferrichrome, aerobactin, and desferrioxamine. An S. aureus mutant defective in the ability to transport ferric hydroxamate complexes was isolated from a Tn917-LTV1 transposon insertion library after sel...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.16.4394-4400.2000

    authors: Sebulsky MT,Hohnstein D,Hunter MD,Heinrichs DE

    更新日期:2000-08-01 00:00:00

  • Linear plasmid SLP2 is maintained by partitioning, intrahyphal spread, and conjugal transfer in Streptomyces.

    abstract::Low-copy-number plasmids generally encode a partitioning system to ensure proper segregation after replication. Little is known about partitioning of linear plasmids in Streptomyces. SLP2 is a 50-kb low-copy-number linear plasmid in Streptomyces lividans, which contains a typical parAB partitioning operon. In S. livid...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01192-09

    authors: Hsu CC,Chen CW

    更新日期:2010-01-01 00:00:00

  • Identification of a novel matrix protein that promotes biofilm maturation in Vibrio fischeri.

    abstract::Bacteria form communities, termed biofilms, in which cells adhere to each other within a matrix, typically comprised of polysaccharides, proteins, and extracellular DNA. Biofilm formation by the marine bacterium Vibrio fischeri requires the Syp polysaccharide, but the involvement of matrix proteins is as yet unknown. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02292-14

    authors: Ray VA,Driks A,Visick KL

    更新日期:2015-02-01 00:00:00

  • Purification and properties of methyl coenzyme M methylreductase from acetate-grown Methanosarcina thermophila.

    abstract::Methyl coenzyme M methylreductase from acetate-grown Methanosarcina thermophila TM-1 was purified 16-fold from a cell extract to apparent homogeneity as determined by native polyacrylamide gel electrophoresis. Ninety-four percent of the methylreductase activity was recovered in the soluble fraction of cell extracts. T...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.8.2481-2487.1991

    authors: Jablonski PE,Ferry JG

    更新日期:1991-04-01 00:00:00

  • Control of dimorphism in Mucor by hexoses: inhibition of hyphal morphogenesis.

    abstract::In anaerobic cultures of Mucor rouxii, morphogenesis was strongly dependent on hexose concentration as well as pCO(2). At low levels of hexose or CO(2), or both, hyphal development occurred; at high levels, the fungus developed as yeast cells. Other dimorphic strains of Mucor responded similarly to hexose and CO(2) bu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.5.1586-1594.1968

    authors: Bartnicki-Garcia S

    更新日期:1968-11-01 00:00:00