Abstract:
:The antibiotic albomycin is highly effective against Streptococcus pneumoniae, with an MIC of 10 ng/ml. The reason for the high efficacy was studied by measuring the uptake of albomycin into S. pneumoniae. Albomycin was transported via the system that transports the ferric hydroxamates ferrichrome and ferrioxamine B. These two ferric hydroxamates antagonized the growth inhibition by albomycin and salmycin. Cross-inhibition of the structurally different ferric hydroxamates to both antibiotics can be explained by the similar iron coordination centers of the four compounds. [(55)Fe(3+)]ferrichrome and [(55)Fe(3+)]ferrioxamine B were taken up by the same transport system into S. pneumoniae. Mutants in the adjacent fhuD, fhuB, and fhuG genes were transport inactive and resistant to the antibiotics. Albomycin, ferrichrome, ferrioxamine B, and salmycin bound to the isolated FhuD protein and prevented degradation by proteinase K. The fhu locus consisting of the fhuD, fhuB, fhuG, and fhuC genes determines a predicted ABC transporter composed of the FhuD binding lipoprotein, the FhuB and FhuG transport proteins, and the FhuC ATPase. It is concluded that active transport of albomycin mediates the high antibiotic efficacy in S. pneumoniae.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Pramanik A,Braun Vdoi
10.1128/JB.00205-06subject
Has Abstractpub_date
2006-06-01 00:00:00pages
3878-86issue
11eissn
0021-9193issn
1098-5530pii
188/11/3878journal_volume
188pub_type
杂志文章abstract::Aeromonas hydrophila AH-3 lateral flagella are not assembled when bacteria grow in liquid media; however, lateral flagellar genes are transcribed. Our results indicate that A. hydrophila lateral flagellar genes are transcribed at three levels (class I to III genes) and share some similarities with, but have many impor...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01994-12
更新日期:2013-04-01 00:00:00
abstract::We report on the cloning and characterization of the rfb gene cluster of the O75 lipopolysaccharide from a urinary tract isolate of Escherichia coli. Deletion cloning defined the minimum region of DNA that expressed the O75 antigen in E. coli host strains to be on a 12.4-kb insert. However, the E. coli strain expressi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.18.5699-5704.1991
更新日期:1991-09-01 00:00:00
abstract::CrO4(2-) resistance in Pseudomonas fluorescens LB300(pLHB1) was related to reduced uptake of CrO4(2-) relative to the plasmidless strain LB303. 51CrO4(2-) was transported mainly via the SO4(2-) active transport system; thus, cells grown with 0.15 mM cysteine, a repressor of the SO4(2-) transport system, were much more...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.8.3853-3856.1987
更新日期:1987-08-01 00:00:00
abstract::The Gram-positive Corynebacterium glutamicum efficiently metabolizes maltose by a pathway involving maltodextrin and glucose formation by 4-α-glucanotransferase, glucose phosphorylation by glucose kinases, and maltodextrin degradation via maltodextrin phosphorylase and α-phosphoglucomutase. However, maltose uptake in ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01629-12
更新日期:2013-06-01 00:00:00
abstract::Grula, Mary M. (Oklahoma State University, Stillwater) and E. A. Grula. Cell division in a species of Erwinia. IV. Metabolic blocks in pantothenate biosynthesis and their relationship to inhibition of cell division. J. Bacteriol. 83:989-997. 1962.-Four compounds that inhibit cell division in an Erwinia sp., d-serine, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.5.989-997.1962
更新日期:1962-05-01 00:00:00
abstract::Following conjugal transfer of the hsdK genes (hsdRK, hsdMK, and hsdSK) of Escherichia coli K-12, restriction activity was first detected only after approximately 15 generations, whereas modification activity was observed immediately. This sequential expression explains the establishment of hsdK genes in a nonmodified...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.15.4905-4906.1993
更新日期:1993-08-01 00:00:00
abstract::The effect of adding hemin to anaerobically grown cells of a strain of Staphylococcus epidermidis, which was heme-deficient due to anaerobic growth, has been examined. Cells grown anaerobically in media containing hemin exhibited a marked increase in several oxidative activities as compared with cells grown anaerobica...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.1.278-285.1967
更新日期:1967-01-01 00:00:00
abstract::A gene (yacK) encoding a putative multicopper oxidase (MCO) was cloned from Escherichia coli, and the expressed enzyme was demonstrated to exhibit phenoloxidase and ferroxidase activities. The purified protein contained six copper atoms per polypeptide chain and displayed optical and electron paramagnetic resonance (E...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.16.4866-4875.2001
更新日期:2001-08-01 00:00:00
abstract::Bacillus subtilis OhrR is the prototype for the one-Cys family of organic peroxide-sensing regulatory proteins. Mutational analyses indicate that the high sensitivity of the active site cysteine (C15) to peroxidation requires three Tyr residues. Y29 and Y40 from the opposing subunit of the functional dimer hydrogen bo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00879-07
更新日期:2007-10-01 00:00:00
abstract::Induction of colicin la at a high temperature (42 C) was demonstrated in a wild strain of Shigella sonnei. After transfer of the Col factor, a similar effect was observed in those recipient cells acquiring colicinogeny. The induction is suggested to be due to the presence of a thermosensitive colicin repressor. The wi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.106.3.720-723.1971
更新日期:1971-06-01 00:00:00
abstract::ATP sulfurylase is a key enzyme in the energy-generating sulfur oxidation pathways of many chemoautotrophic bacteria. The utilization of reduced sulfur compounds to fuel CO2 fixation by the still-uncultured bacterial endosymbionts provides the basis of nutrition in invertebrates, such as the tubeworm Riftia pachyptila...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.12.3723-3729.1994
更新日期:1994-06-01 00:00:00
abstract::Vibrio parahaemolyticus possesses two types of flagella, polar and lateral, powered by distinct energy sources, which are derived from the sodium and proton motive forces, respectively. Although proton-powered flagella in Escherichia coli and Salmonella enterica serovar Typhimurium have been extensively studied, the m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.4.1035-1045.2000
更新日期:2000-02-01 00:00:00
abstract::Cecropins are a family of small, basic antibacterial polypeptides which can be isolated from pupae of immunized Lepidoptera. They are active against both gram-negative and gram-positive bacteria. We studied a mutant of Escherichia coli, strain SB1004, which is more sensitive to cecropin D than is the parental strain. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.154.1.170-176.1983
更新日期:1983-04-01 00:00:00
abstract::Pasteurella multocida is the causative agent of a range of diseases with economic importance in production animals. Many systems have been employed to identify virulence factors of P. multocida, including in vivo expression technology (IVET), signature-tagged mutagenesis, and whole-genome expression profiling. In a pr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.7.2278-2285.2005
更新日期:2005-04-01 00:00:00
abstract::In vivo and in vitro methylation, methanol production assays, and the use of specific antibodies raised against the sensory transducing protein Tar in Escherichia coli all failed to demonstrate the presence of methyl-accepting chemotaxis proteins (MCPs) in the photosynthetic bacterium Rhodobacter sphaeroides, although...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.12.5808-5814.1987
更新日期:1987-12-01 00:00:00
abstract::Cells of Pseudomonas stutzeri are naturally transformed by homologous chromosomal DNA; they do not require chemical treatment to become competent. This capacity to undergo natural transformation was found to be shared by the closely related species P. mendocina, P. alcaligenes, and P. pseudoalcaligenes, but was not de...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.1.93-99.1983
更新日期:1983-01-01 00:00:00
abstract::Bacterial lysis induced by the expression of the cloned lysis gene of the RNA bacteriophage MS2 in Escherichia coli was shown to be under the same regulatory control mechanisms as penicillin-induced lysis. It was controlled by the stringent response and showed the phenomenon of tolerance when E. coli was grown at pH 5...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.11.5027-5033.1988
更新日期:1988-11-01 00:00:00
abstract::Staphylococcus aureus responds to changing extracellular environments in part by adjusting its proteome through alterations of transcriptional priorities and selective degradation of the preexisting pool of proteins. In Bacillus subtilis, the proteolytic adaptor protein MecA has been shown to play a role in assisting ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02222-14
更新日期:2014-12-01 00:00:00
abstract::pGC1, a self-transmissible lac plasmid that originated in Yersinia enterocolitica 842, is described. This plasmid is freely transmissible between strains of Y. enterocolitica and from such strains to strains of Escherichia coli. The plasmid can also be transferref among G. coli strains and back to Y. enterocolitica, a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.127.3.1058-1062.1976
更新日期:1976-09-01 00:00:00
abstract::A systematic search for motifs associated with CcrM DNA methylation sites revealed four long (>100-bp) motifs (CIR sequences) present in up to 21 copies in Caulobacter crescentus. The CIR1 and CIR2 motifs exhibit a conserved inverted repeat organization, with a CcrM site in the center of one of the repeats. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.16.4997-5002.2003
更新日期:2003-08-01 00:00:00
abstract::Although Salmonella typhimurium prefers neutral-pH environments, it can adapt to survive conditions of severe low-pH stress (pH 3.3). The process, termed the acid tolerance response (ATR), includes two distinct stages. The first stage, called pre-acid shock, is induced at pH 5.8 and involves the production of an induc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.7.1981-1987.1993
更新日期:1993-04-01 00:00:00
abstract::The syrA and syrB genes involved in syringomycin production in Pseudomonas syringae pv. syringae B301D were identified from an EcoRI-pLAFR3 cosmid library and then physically and functionally analyzed in relation to plant pathogenicity. Homologous recombination of the genes required for syringomycin production from co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.12.5680-5688.1988
更新日期:1988-12-01 00:00:00
abstract::The region of the colicin E1 polypeptide that interacts with immunity protein has been localized to a 168-residue COOH-terminal peptide. This is the length of a proteolytically generated peptide fragment of colicin E1 against which imm+ function can be demonstrated in osmotically shocked cells. The role of particular ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.1.237-244.1985
更新日期:1985-10-01 00:00:00
abstract::FabG performs the NADPH-dependent reduction of β-keto acyl-acyl carrier protein substrates in the elongation cycle of fatty acid synthesis. We report the characterization of a temperature-sensitive mutation (fabGΔ8) in Escherichia colifabG that results from an in-frame 8-amino-acid residue deletion in the α6/α7 subdom...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00074-17
更新日期:2017-04-25 00:00:00
abstract::A series of studies were performed on a Staphylococcus aureus strain thought to contain a pencillinase plasmid integrated into the host chromosome. Reassociation kinetics analysis of whole-cell deoxyribonucleic acid (DNA) in the presence of pure radioactive plasmid DNA revealed that plasmid-specific sequences were pre...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.3.1424-1428.1975
更新日期:1975-12-01 00:00:00
abstract::The broad-host-range plasmid RK2 is capable of replication and stable maintenance within a wide range of gram-negative bacterial hosts. It encodes the essential replication initiation protein TrfA, which binds to the host initiation protein, DnaA, at the plasmid origin of replication (oriV). There are two versions of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00231-06
更新日期:2006-08-01 00:00:00
abstract::Constitutive beta-glucosidases from Saccharomyces fragilis (Y-18) and S. dobzhanskii (Y-19) precipitated at the same concentration of ammonium sulfate. The partially purified enzymes had similar activation energies, molecular weights, affinities for certain natural and synthetic beta-glucosides, and optimal pH values ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.1.135-141.1967
更新日期:1967-01-01 00:00:00
abstract::The morphology, on MacConkey's agar, of colonies of the bacteria commonly found in enteric specimens is sufficiently characteristic and distinctive, when observed with transmitted oblique illumination and a low-power stereoscopic microscope, to enable identification at the generic (and occasionally the species) level ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.6.1897-1905.1967
更新日期:1967-06-01 00:00:00
abstract::Twenty-six FliF monomers assemble into the MS ring, a central motor component of the bacterial flagellum that anchors the structure in the inner membrane. Approximately 100 amino acids at the C terminus of FliF are exposed to the cytoplasm and, through the interaction with the FliG switch protein, a component of the f...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.5.1624-1633.2003
更新日期:2003-03-01 00:00:00
abstract::Proteus rettgeri and Escherichia coli W were shown to express structurally different penicillin G acylases. The enzymes had similar substrate specificity but differed in molecular weight, isoelectric point, and electrophoretic mobility in polyacrylamide gels and did not antigenically cross-react. When the organisms we...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.163.3.925-932.1985
更新日期:1985-09-01 00:00:00