Genetic and molecular analyses of the gene encoding staphylococcal enterotoxin D.

Abstract:

:The gene (entD) encoding staphylococcal enterotoxin D (SED) has been located on a 27.6-kilobase penicillinase plasmid designated pIB485. This plasmid was present in all SED-producing strains tested. The entD gene was cloned on a 2.0-kilobase DNA fragment and was expressed in Escherichia coli. Sequence analysis of this fragment revealed an open reading frame that encoded a 258-amino-acid protein that possessed a 30-amino-acid signal peptide. The 228-amino-acid mature polypeptide had a molecular weight of 26,360 and contained a high degree of sequence similarity to the other staphylococcal enterotoxins. S1 nuclease mapping showed that transcription of entD was initiated 266 nucleotides upstream from the translation start codon. The entD gene was also shown to be activated by the staphylococcal regulatory element known as agr.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Bayles KW,Iandolo JJ

doi

10.1128/jb.171.9.4799-4806.1989

subject

Has Abstract

pub_date

1989-09-01 00:00:00

pages

4799-806

issue

9

eissn

0021-9193

issn

1098-5530

journal_volume

171

pub_type

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