Abstract:
:The function of AI-2 in many bacteria and the physiological role of LuxS, the enzyme responsible for its production, remain matters of debate. Here, we show that in Staphylococcus aureus the luxS gene forms a monocistronic transcriptional unit under the control of a sigma(70)-dependent promoter. The gene was transcribed throughout growth under a variety of conditions, including intracellular growth in MAC-T cells. AI-2 was produced in rich media under aerobic and anaerobic conditions, peaking during the transition to stationary phase, but was hardly detectable in a sulfur-limited defined medium. In the presence of glucose or under anaerobic conditions, cultures retained considerable AI-2 activity after entry into stationary phase. Inactivation of luxS in various S. aureus strains did not affect virulence-associated traits, such as production of hemolysins and extracellular proteases, biofilm formation, and the agr signaling system. Conversely, AI-2 production remained unchanged in an agr mutant. However, luxS mutants grown in a sulfur-limited defined medium exhibited a growth defect. When grown together with the wild type in mixed culture, luxS mutants of various S. aureus strains showed reduced ability to compete for growth under these conditions. In contrast, a complemented luxS mutant grew as well as the parent strain, suggesting that the observed growth defect was of an intracellular nature and had not been caused by either second-site mutations or the lack of a diffusible factor. However, the LuxS/AI-2 system does not appear to contribute to the overall fitness of S. aureus RN6390B during intracellular growth in epithelial cells: the wild type and a luxS mutant showed very similar growth patterns after their internalization by MAC-T cells.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Doherty N,Holden MT,Qazi SN,Williams P,Winzer Kdoi
10.1128/JB.188.8.2885-2897.2006keywords:
subject
Has Abstractpub_date
2006-04-01 00:00:00pages
2885-97issue
8eissn
0021-9193issn
1098-5530pii
188/8/2885journal_volume
188pub_type
杂志文章abstract::Xenorhabdus nematophilus, a gram-negative bacterium, is a mutualist of Steinernema carpocapsae nematodes and a pathogen of larval-stage insects. We use this organism as a model of host-microbe interactions to identify the functions bacteria require for mutualism, pathogenesis, or both. In many gram-negative bacteria, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.16.4687-4693.2001
更新日期:2001-08-01 00:00:00
abstract::Paenibacillus mucilaginosus is a ubiquitous functional bacterium in microbial fertilizer. Here we report the complete sequence of P. mucilaginosus 3016. Multiple sets of functional genes have been found in the genome. To the best of our knowledge, this is the first announcement about the complete genome sequence of a ...
journal_title:Journal of bacteriology
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doi:10.1128/JB.00323-12
更新日期:2012-05-01 00:00:00
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journal_title:Journal of bacteriology
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abstract::SecA facilitates protein transport across the eubacterial plasma membrane by its association with cargo proteins and the SecYEG translocon, followed by ATP-driven conformational changes that promote protein translocation in a stepwise manner. Whether SecA functions as a monomer or a dimer during this process has been ...
journal_title:Journal of bacteriology
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doi:10.1128/JB.188.1.335-338.2006
更新日期:2006-01-01 00:00:00
abstract::In the course of study on ribonucleic acid (RNA) metabolism during sporulation in Saccharomyces cerevisiae, a new species of RNA (20S) was observed in sporulating cells by polyacrylamide gel electrophoresis. The relative content of this RNA to total RNA increased linearly early in sporulation. Labeled adenine was pref...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.3.826-830.1971
更新日期:1971-03-01 00:00:00
abstract::An electron microscopy study has been made of the effects of dissolution of the plasma membrane of Escherichia coli with sodium dodecyl sulfate (SDS) on the organization of the nucleoplasm and the cytoplasm. The alterations observed in time course experiments were related to absorbance changes and to release of macrom...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.111.3.801-813.1972
更新日期:1972-09-01 00:00:00
abstract::The oral bacterium Streptococcus salivarius takes up lactose via a transporter called LacS that shares 95% identity with the LacS from Streptococcus thermophilus, a phylogenetically closely related organism. S. thermophilus releases galactose into the medium during growth on lactose. Expulsion of galactose is mediated...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.23.6764-6772.2003
更新日期:2003-12-01 00:00:00
abstract::Alcohol-oxidizing enzymes of the facultative methylotroph PAR were investigated after growth of the bacteria on methanol and ethanol. During methanol growth only a phenazine methosulfate-linked alcohol dehydrogenase was detected. This enzyme had broad specificity for primary alcohols and was also capable of oxidation ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.135.1.251-258.1978
更新日期:1978-07-01 00:00:00
abstract::Marchette, Nyven J. (University of Utah, Salt Lake City), and Paul S. Nicholes. Virulence and citrulline ureidase activity of Pasteurella tularensis. J. Bacteriol. 82:26-32. 1961.-The presence of a citrulline ureidase system in Pasteurella tularensis strains of high virulence, and its absence in avirulent strains and ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.82.1.26-32.1961
更新日期:1961-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.114.1.332-340.1973
更新日期:1973-04-01 00:00:00
abstract::The genes encoding the two isoenzymes of methyl coenzyme M reductase (MRI and MRII) in Methanobacterium thermoautotrophicum delta H have been cloned and sequenced. The MRI-encoding mcr operon (mcrBDCGA) has been located immediately upstream from the mtr operon (mtrEDCBA) that encodes N5-methyltetrahydromethanopterin:c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.20.6384-6391.1994
更新日期:1994-10-01 00:00:00
abstract::The nucleic acid binding cold shock proteins (CSPs) and the cold-induced DEAD box RNA helicases have been proposed separately to act as RNA chaperones, but no experimental evidence has been reported on a direct cooperation. To investigate the possible interaction of the putative RNA helicases CshA and CshB and the CSP...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.1.240-248.2006
更新日期:2006-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.106.2.626-633.1971
更新日期:1971-05-01 00:00:00
abstract::Sequencing the insertion sites of 8,865 Tn917 insertions in Enterococcus faecalis strain OG1RF identified a hot spot in the replication terminus region corresponding to 6% of the genome where 65% of the transposons had inserted. In E. faecalis, Tn917 preferentially inserted at a 29-bp consensus sequence centered on TA...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.21.7280-7289.2004
更新日期:2004-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00573-06
更新日期:2006-10-01 00:00:00
abstract::A mutant requiring isoleucine and valine for growth, because of the absence of acetolactate synthase activity, has been isolated. At least one of three different genes (ilvG, ilvB, ilvI) is required for the expression of acetolactate synthase activity, thus suggesting the presence of three different acetolactate synth...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.1.536-538.1974
更新日期:1974-10-01 00:00:00
abstract::The cell walls isolated from axenically grown leprosy-derived corynebacteria were submitted to various chemical and enzymatic degradations. The glycan strands of the wall peptidoglycan are essentially composed of N-acetylglycosaminyl-N-acetylmuramic acid disaccharide units. Small amounts of N-acetylglycosaminyl-N-glyc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.145.2.775-779.1981
更新日期:1981-02-01 00:00:00
abstract::The PmrA/PmrB two-component system encoded by the pmrCAB operon regulates the modification of Salmonella enterica serovar Typhimurium lipopolysaccharide leading to polymyxin B resistance. PmrA and PhoP are the only known activators of pmrCAB. A transposon mutagenesis screen for additional regulators of a pmrC::MudJ fu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.1.141-149.2006
更新日期:2006-01-01 00:00:00
abstract::An open reading frame (slr0899) on the genome of Synechocystis sp. strain PCC 6803 encodes a polypeptide of 149 amino acid residues, the sequence of which is 40% identical to that of cyanase from Escherichia coli. Introduction into a cyanase-deficient E. coli strain of a plasmid-borne slr0899 resulted in expression of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.18.5744-5750.1997
更新日期:1997-09-01 00:00:00
abstract::Cook, Thomas M. (Sterling-Winthrop Research Institute, Rensselaer, N.Y.), William H. Deitz, and William A. Goss. Mechanism of action of nalidixic acid on Escherichia coli. IV. Effects on the stability of cellular constituents. J. Bacteriol. 91:774-779. 1996.-Treatment of Escherichia coli 15TAU with nalidixic acid resu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.2.774-779.1966
更新日期:1966-02-01 00:00:00
abstract::Early log-phase cells of Mycoplasma gallisepticum A5969 were synchronized by holding in Eagle minimal essential medium (MEM) for 2 h. When transferred out of MEM into tryptose medium, the cells exhibited synchronous growth. Deoxyribonucleic acid (DNA) synthesis proceeded continuously during this growth but stopped dur...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.115.1.117-120.1973
更新日期:1973-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.9.2888-2896.2001
更新日期:2001-05-01 00:00:00
abstract::The inhibitory effect of fungitoxic derivatives of 1,4-oxathiin on substrate oxidation by the basidiomycete Ustilago maydis is diminished by a single-gene mutation (oxr). The difference between mutant and wild type is approximately the same on the basis of inhibition of either growth and operation of the tricarboxylic...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.110.3.809-817.1972
更新日期:1972-06-01 00:00:00
abstract::The rates of thermal denaturation and the molecular weights of the two isofunctional enol-lactone hydrolases (ELH I and ELH II) of Acinetobacter calcoaceticus were determined. The molecular weights of ELH I and ELH II were found by gel filtration to be approximately 24,000 and 21,000, respectively. In crude extract at...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.106.2.369-374.1971
更新日期:1971-05-01 00:00:00
abstract::MalT is the central transcriptional activator of all mal genes in Escherichia coli. Its activity is controlled by the inducer maltotriose. It can be inhibited by the interaction with certain proteins, and its expression can be controlled. We report here a novel aspect of mal gene regulation: the effect of cytoplasmic ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00767-08
更新日期:2009-02-01 00:00:00
abstract::sigma E is a sporulation-specific sigma factor of Bacillus subtilis that is formed from an inactive precursor protein (pro-sigma E) by the removal of 27 to 29 amino acids from the pro-sigma E amino terminus. By using oligonucleotide-directed mutagenesis, sequential deletions were constructed in the precursor-specific ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.14.4629-4637.1992
更新日期:1992-07-01 00:00:00
abstract::We isolated a new recF mutant of Escherichia coli K-12 by insertion of transposon Tn5 into the recF gene. This recF400::Tn5 allele displayed the same phenotypic characteristics as the classic recF143 mutation. By using Mu d(Ap lac) fusions, the induction of nine SOS genes, including recA, umuC, dinA, dinB, dinD, dinF,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.4.1731-1736.1987
更新日期:1987-04-01 00:00:00
abstract::Adaptation of Lactococcus lactis towards progressive carbon starvation is mediated by three different types of transcriptomic responses: (i) global responses, i.e., general decreases of functions linked to bacterial growth and lack of induction of the general stress response; (ii) specific responses functionally relat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.10.3589-3592.2005
更新日期:2005-05-01 00:00:00
abstract::Transcriptional regulation of the Mg(2+)-citrate transporter, CitM, the main citrate uptake system of Bacillus subtilis, was studied during growth in rich medium. Citrate in the growth medium was required for induction under all growth conditions. In Luria-Bertani medium containing citrate, citM expression was complet...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.3.854-859.2003
更新日期:2003-02-01 00:00:00
abstract::Mir M7 is a spontaneous morphologically conditional mutant of Klebsiella pneumoniae which grows as round cells (cocci) at pH 7 and as normal rods at pH 5.8. We studied the rates of peptidoglycan synthesis of cocci and rods growing at pH values of 7 and 5.8, respectively. It was found that exponentially growing cocci p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.137.2.727-734.1979
更新日期:1979-02-01 00:00:00