Mutational analysis of the precursor-specific region of Bacillus subtilis sigma E.

abstract：:The complete nucleotide sequence of the linear plasmid pBD2 from Rhodococcus erythropolis BD2 comprises 210,205 bp. Sequence analyses of pBD2 revealed 212 putative open reading frames (ORFs), 97 of which had an annotatable function. These ORFs could be assigned to six functional groups: plasmid replication and mainten...

journal_title：Journal of bacteriology

authors： Stecker C,Johann A,Herzberg C,Averhoff B,Gottschalk G

更新日期：2003-09-01 00:00:00

abstract：:Cells of Ureaplasma urealyticum that were prepared by a ruthenium red technique demonstrated an extramembranous layer of polyanions of about 15 to 30 nm in width. Application of the concanavalin A-iron dextran strain indicated that the outer surface of this layer contained glucosyl-like residues. ...

journal_title：Journal of bacteriology

authors： Robertson J,Smook E

更新日期：1976-11-01 00:00:00

abstract：:The gene encoding the outer membrane phosphate-selective porin protein P from Pseudomonas aeruginosa was cloned into Escherichia coli. The protein product was expressed and transported to the outer membrane of an E. coli phoE mutant and assembled into functional trimers. Expression of a product of the correct molecula...

journal_title：Journal of bacteriology

authors： Siehnel RJ,Worobec EA,Hancock RE

更新日期：1988-05-01 00:00:00

abstract：:An examination of the methanofurans isolated from a wide range of methanogenic bacteria and from Archaeoglobus fulgidus has revealed at least five chromatographically distinct methanofurans. Bacteria from each major genus of methanogenic bacteria have been found to contain a chemically different methanofuran. The natu...

journal_title：Journal of bacteriology

authors： White RH

更新日期：1988-10-01 00:00:00

abstract：:[3H]Diaminopimelic acid (Dap) was incorporated exclusively into peptidoglycan by Escherichia coli strains auxotrophic for both lysine and Dap. The rate of [3H]Dap incorporation by stringent (rel+) strains was significantly decreased when cells were deprived of required amino acids. The addition of chloramphenicol to a...

journal_title：Journal of bacteriology

authors： Ishiguro EE,Ramey WD

更新日期：1976-09-01 00:00:00

abstract：:Strains derived from HfrH carrying the arcA2 null mutation exhibit a higher respiratory rate, enhanced glucose consumption, and a more-reduced intracellular redox state than arcA deletion mutants of a different lineage. The phenotype of the arcA2 mutants was due to the presence of a creC constitutive mutation introduc...

journal_title：Journal of bacteriology

authors： Nikel PI,de Almeida A,Pettinari MJ,Méndez BS

更新日期：2008-05-01 00:00:00

abstract：:MobB is a small protein encoded by the broad-host-range plasmid R1162 and required for efficient mobilization of its DNA during conjugation. The protein was shown previously to stabilize the relaxosome, the complex of plasmid DNA and mobilization proteins at the origin of transfer (oriT). We have generated in-frame mo...

journal_title：Journal of bacteriology

authors： Perwez T,Meyer RJ

更新日期：1999-04-01 00:00:00

abstract：:In the acetoclastic methanogen Methanothrix soehngenii, acetate is activated to acetyl coenzyme A by acetyl coenzyme A synthetase (Acs). The acs gene, coding for the single Acs subunit, was isolated from a genomic library of M. soehngenii DNA in Escherichia coli by using antiserum raised against the purified Acs. Afte...

journal_title：Journal of bacteriology

authors： Eggen RI,Geerling AC,Boshoven AB,de Vos WM

更新日期：1991-10-01 00:00:00

abstract：:The type II secretion system (main terminal branch of the general secretion pathway) is used by diverse gram-negative bacteria to secrete extracellular proteins. Proteins secreted by this pathway are synthesized with an N-terminal signal peptide which is removed upon translocation across the inner membrane, but the si...

journal_title：Journal of bacteriology

authors： Lindeberg M,Boyd CM,Keen NT,Collmer A

更新日期：1998-03-01 00:00:00

abstract：:Postembedding staining of intracellular carbohydrates on thin sections of Staphylococcus aureus was studied by the silver methenamine and the wheat germ agglutinin-gold techniques. Staining of silver grains was observed on both the cell wall and the cross wall. The staining was interpreted to be due to teichoic acid. ...

journal_title：Journal of bacteriology

authors： Morioka H,Tachibana M,Suganuma A

更新日期：1987-03-01 00:00:00

abstract：:In response to molecular oxygen and/or fixed nitrogen, the product of the Klebsiella pneumoniae nitrogen fixation L (nifL) gene inhibits NifA-mediated transcriptional activation. Nitrogen regulation of NifL function occurs at two levels: transcription of the nifLA operon is regulated by the general Ntr system, and the...

journal_title：Journal of bacteriology

authors： He L,Soupene E,Kustu S

更新日期：1997-12-01 00:00:00

abstract：:The filamentous cyanobacterium Anabaena sp. strain PCC 7120 forms a periodic pattern of nitrogen-fixing heterocysts when grown in the absence of combined nitrogen. PatA is necessary for proper patterning of heterocysts along filaments. In this study, apparent transcriptional start points (tsps) were identified at nucl...

journal_title：Journal of bacteriology

authors： Young-Robbins SS,Risser DD,Moran JR,Haselkorn R,Callahan SM

更新日期：2010-09-01 00:00:00

abstract：:Eight mutants of Bacillus subtilis deficient in xylan utilization were isolated and characterized genetically and biochemically. Each mutant was obtained independently after nitrosoguanidine mutagenesis. All of the analyzed mutations were shown to be linked. Reciprocal transformation crosses revealed the existence of ...

journal_title：Journal of bacteriology

authors： Roncero MI

更新日期：1983-10-01 00:00:00

abstract：:By extracellular labeling of peptides of intact Corynebacterium diphtheriae, followed by fractionation of the cells and chain completion by isolated polysomes, it is shown that diphtheria toxin is formed and secreted cotranslationally by membrane-bound polysomes; free polysomes from none. Moreover, when the chains on ...

journal_title：Journal of bacteriology

authors： Smith WP,Tai PC,Murphy JR,Davis BD

更新日期：1980-01-01 00:00:00

abstract：:The inability of Micrococcus sodonensis to grow on glucose as the sole source of carbon and energy was investigated. Estimation of pathways of glucose catabolism indicated that both the glycolytic and hexose monophosphate pathways are present in this organism. Comparative studies with Escherichia coli demonstrated tha...

journal_title：Journal of bacteriology

authors： Perry JJ,Evans JB

更新日期：1967-06-01 00:00:00

abstract：:Three spontaneous derivatives of Streptococcus faecium ATCC 9790, originally isolated as conditionally Triton X-100 detergent-resistant at 25 degrees C, displayed normal penicillin-induced rates of lysis at 37 degrees C but substantially reduced rates of lysis and killing at 25 degrees C. The addition of exogenous uns...

journal_title：Journal of bacteriology

authors： Pucci MJ,Daneo-Moore L

更新日期：1984-08-01 00:00:00

abstract：:The restriction systems McrA and McrB of Escherichia coli K-12 are known to attack DNA containing modified cytosine. In strains lacking both activities, however, we observed that DNA methylated at CG dinucleotides (as is mammalian DNA) was still significantly restricted. We show that this substantial barrier to the ac...

journal_title：Journal of bacteriology

authors： Kelleher JE,Raleigh EA

更新日期：1991-08-01 00:00:00

abstract：:MntH, a bacterial homolog of mammalian natural resistance associated macrophage protein 1 (Nramp1), is a primary transporter for Mn(2+) influx in Salmonella enterica serovar Typhimurium and Escherichia coli. S. enterica serovar Typhimurium MntH contributes to H(2)O(2) resistance and is important for full virulence. Co...

journal_title：Journal of bacteriology

authors： Kehres DG,Janakiraman A,Slauch JM,Maguire ME

更新日期：2002-06-01 00:00:00

abstract：:A Flavobacterium sp. (strain 50001), capable of degrading 2,4-dichlorophenoxyacetate (2,4-D), 2-methyl-4-chlorophenoxyacetate, and 2-chlorobenzoate and imparting resistance to mercury, harbored a degradative plasmid, pRC10. Cured strains of the Flavobacterium sp. lost the plasmid as well as the ability to degrade thes...

journal_title：Journal of bacteriology

authors： Chaudhry GR,Huang GH

更新日期：1988-09-01 00:00:00

abstract：:PcsB is a protein of unknown function that plays a critical role in cell division in Streptococcus pneumoniae and other ovococcus species of Streptococcus. We constructed isogenic sets of mutants expressing different amounts of PcsB in laboratory strain R6 and virulent serotype 2 strain D39 to evaluate its cellular ro...

journal_title：Journal of bacteriology

authors： Barendt SM,Land AD,Sham LT,Ng WL,Tsui HC,Arnold RJ,Winkler ME

更新日期：2009-05-01 00:00:00

abstract：:We have cloned a lytic enzyme, PlyPH, with a specific lytic effect on Bacillus anthracis strains. PlyPH remains active between pH 4 and 10.5, and a single dose rescued a significant percentage of mice infected intraperitoneally with an attenuated B. anthracis strain. We propose PlyPH as a novel therapeutic agent. ...

journal_title：Journal of bacteriology

authors： Yoong P,Schuch R,Nelson D,Fischetti VA

更新日期：2006-04-01 00:00:00

abstract：:Chorpenning, Frank W. (The Ohio State University, Columbus), and Matthew C. Dodd. Heterogenetic antigens of gram-positive bacteria. J. Bacteriol. 91:1440-1445. 1966.-Soluble antigens obtained by various methods from gram-positive bacteria were used to modify erythrocytes whose hemagglutinating reactions with immune ra...

journal_title：Journal of bacteriology

authors： Chorpenning FW,Dodd MC

更新日期：1966-04-01 00:00:00

abstract：:Ornithine carbamoyltransferase synthesis is subject to two major regulatory systems in Saccharomyces cerevisiae. One system is specific for the arginine biosynthetic enzymes, whereas the other appears to be general, acting on a variety of other amino acid pathways as well. We observed that the synthetic capacity for c...

journal_title：Journal of bacteriology

authors： Messenguy F,Cooper TG

更新日期：1977-06-01 00:00:00

abstract：:Type 1 pili of Escherichia coli are the prototype of the somatic class of pili found on many strains of bacteria. As a first step in the genetic analysis of type 1 piliation, an extensive series of nonpiliated derivatives of E. coli K-12 strain AW405, was characterized to produce attached or free pili when examined in...

journal_title：Journal of bacteriology

authors： Swaney LM,Liu YP,To CM,To CC,Ippen-Ihler K,Brinton CC Jr

更新日期：1977-04-01 00:00:00

abstract：:Rhodopseudomonas capsulata required potassium (or rubidium or cesium as analogs of potassium) for growth. These cations were actively accumulated by the cells by a process following Michaelis-Menten saturation kinetics. The monovalent cation transport system had Km's of 0.2 mM K+, 0.5 mM Rb+, and 2.6 mM Cs+. The rates...

journal_title：Journal of bacteriology

authors： Jasper P

更新日期：1978-03-01 00:00:00

abstract：:The Arc (anoxic redox control) two-component signal transduction system, consisting of the ArcB sensor kinase and the ArcA response regulator, allows adaptive responses of Escherichia coli to changes of O(2) availability. The arcA gene was previously known as the dye gene because null mutants were growth sensitive to ...

journal_title：Journal of bacteriology

authors： Alvarez AF,Malpica R,Contreras M,Escamilla E,Georgellis D

更新日期：2010-01-01 00:00:00

abstract：:The nucleotide sequence of a 3.4-kb EcoRI-PstI DNA fragment of Xanthomonas campestris pv. campestris revealed two open reading frames, which were designated xanA and xanB. The genes xanA and xanB encode proteins of 448 amino acids (molecular weight of 48,919) and 466 amino acids (molecular weight of 50,873), respectiv...

journal_title：Journal of bacteriology

authors： Köplin R,Arnold W,Hötte B,Simon R,Wang G,Pühler A

更新日期：1992-01-01 00:00:00

abstract：:Site-directed mutagenesis has been employed to address the functional significance of the highly conserved aspartic and glutamic acid residues present in the Walker B (also called motif II) sequence in Escherichia coli DNA helicase II. Two mutant proteins, UvrDE221Q and UvrDD220NE221Q, were expressed and purified to a...

journal_title：Journal of bacteriology

authors： Brosh RM Jr,Matson SW

更新日期：1995-10-01 00:00:00

abstract：:Respiration-deficient mutants (Rho-, petite) of Saccharomyces carlsbergensis were obtained by treatment with trypaflavin (euflavine). Dried cells of these mutants phosphorylated mononucleotides to their triphosphates and further formed not only cytidine 5'-diphosphate-choline, but also sugar nucleotides, such as uridi...

journal_title：Journal of bacteriology

authors： Kimura A,Hirose K,Kariya Y,Nagai S

更新日期：1976-02-01 00:00:00

abstract：:Prokaryotic organisms occupy the most diverse set of environments and conditions on our planet. Their ability to sense and respond to a broad range of external cues remain key research areas in modern microbiology, central to behaviors that underlie beneficial and pathogenic interactions of bacteria with multicellular...

journal_title：Journal of bacteriology

authors： Watts KJ,Vaknin A,Fuqua C,Kazmierczak BI

更新日期：2019-09-20 00:00:00