Complete nucleotide sequence and genetic organization of the 210-kilobase linear plasmid of Rhodococcus erythropolis BD2.

abstract：:Protoplasts of Saccharomyces cerevisiae were mixed with linear DNA plasmids, pGKl1 and pGKl2, isolated from a Kluyveromyces lactis killer strain and treated with polyethylene glycol. Out of 2,000 colonies regenerated on a nonselective medium, two killer transformants were obtained. The pGKl plasmids and the killer cha...

journal_title：Journal of bacteriology

authors： Gunge N,Murata K,Sakaguchi K

更新日期：1982-07-01 00:00:00

abstract：:Most iron in mammals is found within the heme prosthetic group. Consequently, many bacterial pathogens possess heme acquisition systems to utilize iron from the host. Here, we demonstrate that Mycobacterium tuberculosis can utilize heme as an iron source, suggesting that M. tuberculosis possesses a yet-unknown heme ac...

journal_title：Journal of bacteriology

authors： Jones CM,Niederweis M

更新日期：2011-04-01 00:00:00

abstract：:We have devised a coupled radiochemical assay for detecting phosphatidylglycerolphosphate (PGP) phosphatase activity in Escherichia coli colonies immobilized on filter paper. There appeared to be at least two enzymes capable of dephosphorylating PGP, as judged by the characterization of mutations in two genes designat...

journal_title：Journal of bacteriology

authors： Icho T,Raetz CR

更新日期：1983-02-01 00:00:00

abstract：:Previous studies have implicated glutamine synthetase (L-glutamate:ammonia ligase [adenosine diphosphate for-ing], EC 6.6.1.2) as a major controlling element of the nitrogen fixation (nif) genes in Klebsiella pneumoniae. We report here the isolation of a new class of K. pneumoniae mutants which exhibit altered pattern...

journal_title：Journal of bacteriology

authors： Ausubel FM,Bird SC,Durbin KJ,Janssen KA,Margolskee RF,Peskin AP

更新日期：1979-11-01 00:00:00

abstract：:Host range mutants of phage T1 (T1h), which productively infected tonB mutants of Escherichia coli, were isolated. The phage mutants were inactivated by isolated outer membranes of E. coli in contrast to the wild-type phage, which only adsorbed reversibly. For the infection process, the tonB function is apparently onl...

journal_title：Journal of bacteriology

authors： Hantke K,Braun V

更新日期：1978-07-01 00:00:00

abstract：:Campylobacter jejuni produces both lipooligosaccharide (LOS) and a higher-molecular-weight polysaccharide that is believed to form a capsule. The role of these surface polysaccharides in C. jejuni-mediated enteric disease is unclear; however, epitopes associated with the LOS are linked to the development of neurologic...

journal_title：Journal of bacteriology

authors： Oldfield NJ,Moran AP,Millar LA,Prendergast MM,Ketley JM

更新日期：2002-04-01 00:00:00

abstract：:The rates of thermal denaturation and the molecular weights of the two isofunctional enol-lactone hydrolases (ELH I and ELH II) of Acinetobacter calcoaceticus were determined. The molecular weights of ELH I and ELH II were found by gel filtration to be approximately 24,000 and 21,000, respectively. In crude extract at...

journal_title：Journal of bacteriology

authors： Katagiri M,Wheelis ML

更新日期：1971-05-01 00:00:00

abstract：:Nucleoside diphosphate kinase (Ndk) is a ubiquitous enzyme which functions in balancing the nucleotide pool of the cell. We have recently reported that in addition to being intracellular in both mucoid and nonmucoid Pseudomonas aeruginosa, Ndk is also secreted into the extracellular environment by mucoid P. aeruginosa...

journal_title：Journal of bacteriology

authors： Kamath S,Chen ML,Chakrabarty AM

更新日期：2000-07-01 00:00:00

abstract：:An R plasmid, R100-1, was mapped previously (Yoshikawa, 1974) by transduction from an integratively suppressed Hfr strain to a recipient with a mutation in gene dnaA. By this method various types of transductants of plasmid R100-1 that exist autonomously or in the integrated state were obtained. Seventy-one such trans...

journal_title：Journal of bacteriology

authors： Yoshikawa M

更新日期：1975-12-01 00:00:00

abstract：:Transcription of the tryptophanase (tna) operon of Escherichia coli is regulated by catabolite repression and tryptophan-induced transcription antitermination. Induction results from ribosome stalling after translation of tnaC, the coding region for a 24-residue leader peptide. The last sense codon of tnaC, proline co...

journal_title：Journal of bacteriology

authors： Gong M,Gong F,Yanofsky C

更新日期：2006-03-01 00:00:00

abstract：:The morphology of nucleoids and mesosomes of Bacillus subtilis in stationary-and lag-phase cultures was studied by making three-dimensional cell reconstructions in plastic of electron micrographs of serial sections. In cells from stationary cultures, the dormant nucleoids are frequently, but not always, spherical and ...

journal_title：Journal of bacteriology

authors： Van Iterson W,Michels PA,Vyth-Dreese F,Aten JA

更新日期：1975-03-01 00:00:00

abstract：:Utilization of heme as an iron source by Bradyrhizobium japonicum involves induction of the outer membrane heme receptor gene hmuR and other genes within the heme utilization locus. Here, we discovered the hmuP gene located upstream of hmuR and transcribed divergently from it along with hmuTUV. hmuP encodes a small pr...

journal_title：Journal of bacteriology

authors： Escamilla-Hernandez R,O'Brian MR

更新日期：2012-06-01 00:00:00

abstract：:The Escherichia coli LytB protein regulates the activity of guanosine 3',5'-bispyrophosphate synthetase I (RelA). A Southern blot analysis of chromosomal DNA with the E. coli lytB gene as a probe revealed the presence of lytB homologs in all of the gram-negative bacterial species examined but not in gram-positive spec...

journal_title：Journal of bacteriology

authors： Potter S,Yang X,Boulanger MJ,Ishiguro EE

更新日期：1998-04-01 00:00:00

abstract：:Containment strategies for outbreaks of invasive Neisseria meningitidis disease are informed by serogroup assays that characterize the polysaccharide capsule. We sought to uncover the genomic basis of conflicting serogroup assay results for an isolate (M16917) from a patient with acute meningococcal disease. To this e...

journal_title：Journal of bacteriology

authors： Rishishwar L,Katz LS,Sharma NV,Rowe L,Frace M,Dolan Thomas J,Harcourt BH,Mayer LW,Jordan IK

更新日期：2012-10-01 00:00:00

abstract：:In Rhizobium meliloti, an aspartate aminotransferase (AspAT) encoded within a 7.3-kb HindIII fragment was previously shown to be required for symbiotic nitrogen fixation and aspartate catabolism (V. K. Rastogi and R.J. Watson, J. Bacteriol. 173:2879-2887, 1991). A gene coding for an aromatic aminotransferase located w...

journal_title：Journal of bacteriology

authors： Watson RJ,Rastogi VK

更新日期：1993-04-01 00:00:00

abstract：:Peripheral hyphae were separated from the remaining thallus of Rhizoctonia solani in exponential and stationary phases of growth. The QO(2) in whole cells of peripheral hyphae from young fungal colonies was on the average 2.6 times and the protein content 1.6 times greater than in peripheral hyphae from old fungal col...

journal_title：Journal of bacteriology

authors： Skowronski BS,Gottlieb D

更新日期：1970-11-01 00:00:00

abstract：:We report the use of a fusion to the green fluorescent protein to visualize the assembly of the morphogenetic protein SpoIVA around the developing forespore during the process of sporulation in the bacterium Bacillus subtilis. Using a deconvolution algorithm to process digitally-collected optical sections, we show tha...

journal_title：Journal of bacteriology

authors： Price KD,Losick R

更新日期：1999-02-01 00:00:00

abstract：:Protein phosphorylation was demonstrated in Bradyrhizobium japonicum bacteroids in vivo and in cultures in vivo and in vitro. Comparison of in vivo-labeled phosphoproteins of bacteroids and of cultured cells showed differences in both the pattern and intensity of labeling. In cultured cells, comparison of the labeling...

journal_title：Journal of bacteriology

authors： Karr DB,Emerich DW

更新日期：1989-06-01 00:00:00

abstract：:The positions of the outer boundaries of the 5'- and 3'-conserved segment sequences of integrons found at several different locations have been determined. The position of the 5' end of the 5'-conserved segment is the same for six independently located integrons, In1 (R46), In2 (Tn21), In3 (R388), In4 (Tn1696), In5 (p...

journal_title：Journal of bacteriology

authors： Hall RM,Brown HJ,Brookes DE,Stokes HW

更新日期：1994-10-01 00:00:00

abstract：:Site-specific mutagenesis has identified amino acids involved in bR proton transport. Biophysical studies of the mutants have elucidated the roles of two membrane-embedded residues: Asp-85 serves as the acceptor for the proton from the isomerized retinylidene Schiff base, and Asp-96 participates in reprotonation of th...

journal_title：Journal of bacteriology

authors： Krebs MP,Khorana HG

更新日期：1993-03-01 00:00:00

abstract：:The RimM protein in Escherichia coli is associated with free 30S ribosomal subunits but not with 70S ribosomes. A DeltarimM mutant shows a sevenfold-reduced growth rate and a reduced translational efficiency, probably as a result of aberrant assembly of the ribosomal 30S subunits. The slow growth and translational def...

journal_title：Journal of bacteriology

authors： Bylund GO,Lövgren JM,Wikström PM

更新日期：2001-10-01 00:00:00

abstract：:Southern blot analysis indicated that the gene encoding the constitutive, NADP-linked bile acid 7 alpha-hydroxysteroid dehydrogenase of Eubacterium sp. strain VPI 12708 was located on a 6.5-kb EcoRI fragment of the chromosomal DNA. This fragment was cloned into bacteriophage lambda gt11, and a 2.9-kb piece of this ins...

journal_title：Journal of bacteriology

authors： Baron SF,Franklund CV,Hylemon PB

更新日期：1991-08-01 00:00:00

abstract：:Here, we present the complete genome sequence of Rhodococcus pyridinivorans AK37 strain NCAIM PB1376, which was isolated from an oil-polluted site in Hungary. R. pyridinivorans AK37 is an aerobic, nonsporulating, nonmotile, gram-positive bacterium with remarkable aromatic-decomposing activity. ...

journal_title：Journal of bacteriology

authors： Kriszt B,Táncsics A,Cserháti M,Tóth Á,Nagy I,Horváth B,Nagy I,Tamura T,Kukolya J,Szoboszlay S

更新日期：2012-03-01 00:00:00

abstract：:A new type of temperature-sensitive deoxyribonucleic acid (DNA) synthesis mutant, which can divide without a completion of DNA replication, was isolated from a thymidine-requiring Escherichia coli strain by means of photo-bromouracil selection after nitrosoguanidine mutagenesis. In this mutant, in spite of the fact th...

journal_title：Journal of bacteriology

authors： Inouye M

更新日期：1969-09-01 00:00:00

abstract：:Oligoribonuclease, an exoribonuclease specific for small oligoribonucleotides, was initially characterized 20 years ago (S. K. Niyogi and A. K. Datta, J. Biol. Chem. 250:7307-7312, 1975) and shown to be different from RNase II and polynucleotide phosphorylase. Here we demonstrate, using mutant strains and purified enz...

journal_title：Journal of bacteriology

authors： Yu D,Deutscher MP

更新日期：1995-07-01 00:00:00

abstract：:Altenbern, Robert A. (U.S. Army Biological Laboratories, Fort Detrick, Frederick, Md.). Growth of L forms of streptomycin-dependent Proteus mirabilis. J. Bacteriol. 85:619-622. 1963.-A streptomycin-dependent mutant of Proteus mirabilis produces L forms on penicillin-containing medium when dihydrostreptomycin is also p...

journal_title：Journal of bacteriology

authors： ALTENBERN RA

更新日期：1963-03-01 00:00:00

abstract：:During sporulation, the Bacillus subtilis transcription factor sigmaK is activated by regulated proteolytic processing. I have used a system that facilitates the analysis of the contributions of a modified form of the processing enzyme, SpoIVFB-GFP, and the regulatory proteins BofA and SpoIVFA to the conversion of pro...

journal_title：Journal of bacteriology

authors： Resnekov O

更新日期：1999-09-01 00:00:00

abstract：:Well-characterized Salmonella mutants formerly used in biosynthetic studies of lipopolysaccharides were used to study the toxic portion of the complex endotoxin. Endotoxins prepared from wild types and their mutants were tested for their biological activities, including pyrogenicity, lethality, and immunogenicity. The...

journal_title：Journal of bacteriology

authors： Kim YB,Watson DW

更新日期：1967-11-01 00:00:00

abstract：:Bacteroides fragilis synthesizes eight distinct capsular polysaccharides, more than any described bacterium outside the order Bacteroidales. Here, we show that this organism also produces a high-molecular-weight extracellular polysaccharide (EPS). Expression of the EPS results in the formation of a large polysaccharid...

journal_title：Journal of bacteriology

authors： Chatzidaki-Livanis M,Coyne MJ,Roche-Hakansson H,Comstock LE

更新日期：2008-02-01 00:00:00

abstract：:A split UV light dose procedure was used in Escherichia coli to induce an SOS function, RecA protein amplification, which was measured by an immunoradiometric assay. The SOS system was partially induced after the first UV irradiation, and the inducing effects of subsequent identical UV doses were quantified. Variation...

journal_title：Journal of bacteriology

authors： Calsou P,Salles B

更新日期：1985-06-01 00:00:00