Abstract:
:MobB is a small protein encoded by the broad-host-range plasmid R1162 and required for efficient mobilization of its DNA during conjugation. The protein was shown previously to stabilize the relaxosome, the complex of plasmid DNA and mobilization proteins at the origin of transfer (oriT). We have generated in-frame mobB deletions that specifically inactivate the stabilizing effect of MobB while still allowing a high rate of transfer. Thus, MobB has two genetically distinct functions in transfer. The effect of another deletion, extending into mobA, indicates that both functions require a specific region of MobA protein that is distinct from the nicking-ligating domain. The mobB mutations that specifically affected stability also resulted in poor growth of cells, due to increased transcription from the promoters adjacent to oriT. The effects of the mutations could be suppressed not only by full-length MobB provided in trans, as expected, but also by additional copies of oriT, cloned in pBR322. In addition, in the presence of MobA both the full-length and truncated forms of MobB stimulated recombination between oriT-containing plasmids. We propose a model in which MobB regulates expression of plasmid genes by altering the stability of the relaxosome, in a manner that involves the coupling of plasmid molecules.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Perwez T,Meyer RJdoi
10.1128/JB.181.7.2124-2131.1999keywords:
subject
Has Abstractpub_date
1999-04-01 00:00:00pages
2124-31issue
7eissn
0021-9193issn
1098-5530journal_volume
181pub_type
杂志文章abstract::Wiley, W. R. (Washington State University, Pullman) and J. L. Stokes. Requirement of an alkaline pH and ammonia for substrate oxidation by Bacillus pasteurii. J. Bacteriol. 84:730-734. 1962-Resting-cell suspensions of Bacillus paseurii require a pH higher than 8.0 and also NH(4) (+) for the oxidation of glycine, alani...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.4.730-734.1962
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journal_title:Journal of bacteriology
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doi:10.1128/jb.175.13.3934-3940.1993
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.110.3.809-817.1972
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pub_type: 杂志文章
doi:10.1128/JB.133.3.1181-1189.1978
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更新日期:1998-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1999-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:1967-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.148.2.728-729.1981
更新日期:1981-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1989-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1987-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2001-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.145.2.1095-1098.1981
更新日期:1981-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1979-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2010-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1976-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2003-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2006-04-01 00:00:00
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更新日期:1993-03-01 00:00:00
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更新日期:1997-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1971-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1999-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1980-05-01 00:00:00