Limited role for the DsrA and RprA regulatory RNAs in rpoS regulation in Salmonella enterica.

abstract：:The spx gene encodes an RNA polymerase-binding protein that exerts negative and positive transcriptional control in response to oxidative stress in Bacillus subtilis. It resides in the yjbC-spx operon and is transcribed from at least five promoters located in the yjbC regulatory region or in the yjbC-spx intergenic re...

journal_title：Journal of bacteriology

authors： Leelakriangsak M,Kobayashi K,Zuber P

更新日期：2007-03-01 00:00:00

abstract：:Of 16 restriction endonucleases known to hydrolyze rare 6- or 8-base recognition sequences that were tested, only SpeI, NotI, AscI, and SfiI generated fragments of chromosomal DNA from Yersinia pestis, the causative agent of bubonic plague, of sufficient length to permit physical analysis by pulsed-field gel electroph...

journal_title：Journal of bacteriology

authors： Lucier TS,Brubaker RR

更新日期：1992-04-01 00:00:00

abstract：:In this study, we identified and characterized the enzymatic properties of MG_186, a calcium-dependent Mycoplasma genitalium nuclease. MG_186 displays the hallmarks of nucleases, as indicated by its amino acid sequence similarity to other nucleases. We cloned, UGA corrected, expressed, purified, and demonstrated that ...

journal_title：Journal of bacteriology

authors： Li L,Krishnan M,Baseman JB,Kannan TR

更新日期：2010-10-01 00:00:00

abstract：:A spontaneously arising regulatory mutant of the gluconate system in Escherichia coli was isolated. This mutant became constitutive, probably in one step, for gluconate high-affinity transport, gluconokinase, and gluconate-6-P dehydrase. The mutation involved (gntR18) is cotransducible with asd. Pseudorevertants, deri...

journal_title：Journal of bacteriology

authors： Zwaig N,Nagel de Zwaig R,Istúriz T,Wecksler M

更新日期：1973-05-01 00:00:00

abstract：:Intact spheroplasts of the cyanobacterium Anacystis nidulans were found to oxidize various exogenous c-type cytochromes with concomitant proton extrusion. In the coupled state, H+/e stoichiometries close to 1 were measured, regardless of absolute reaction rates. It is concluded that the proton translocation observed i...

journal_title：Journal of bacteriology

authors： Peschek GA

更新日期：1983-01-01 00:00:00

abstract：:Recombinant Escherichia coli strains harboring heterologous polyhydroxyalkanoate (PHA) biosynthesis genes were shown to accumulate unusually large amounts of PHA. In the present study, integrated cellular responses of metabolically engineered E. coli to the accumulation of poly(3-hydroxybutyrate) (PHB) in the early st...

journal_title：Journal of bacteriology

authors： Han MJ,Yoon SS,Lee SY

更新日期：2001-01-01 00:00:00

abstract：:In Salmonella typhimurium, three groups of genes located in rfb, rfe, and rff clusters are known to be involved in the biosynthesis of the enterobacterial common antigen. We found that enzymatic synthesis of uridine diphosphate N-acetylmannosaminouric acid, the activated form of a constituent sugar of the common antig...

journal_title：Journal of bacteriology

authors： Lew HC,Nikaido H,Mäkelä PH

更新日期：1978-10-01 00:00:00

abstract：:The effect of the ilvG671, ilvG468, and ilvG603 mutations (phenotype, IlvG+ Valr; formerly ilvO) upon proteins synthesized was determined by infection of irradiated Escherichia coli K-12 cells, using specifically constructed derivatives of lambda dilv phage. These ilvG alleles are similar to the previously studied ilv...

journal_title：Journal of bacteriology

authors： Gray JE,Wallen JW,Calhoun DH

更新日期：1982-07-01 00:00:00

abstract：:A mutant of yeast lacking proteinase C (carboxypeptidase Y) activity has been found by using a histochemical stain to screen mutagenized colonies. This defect segregates 2:2 in meiotic tetrads. Cell extracts lacked the esterolytic, amidase, and proteolytic activities associated with proteinase C. The absence of protei...

journal_title：Journal of bacteriology

authors： Wolf DH,Fink GR

更新日期：1975-09-01 00:00:00

abstract：:Cell division of Escherichia coli K-12 strain PA3092 was inhibited by the addition of adenosine 3',5'-phosphate (cAMP), and the cellular morphology was changed from rods into filaments. Nucleoids in the filaments were regularly distributed and septum formation was perfectly inhibited. This inhibition of cell division ...

journal_title：Journal of bacteriology

authors： Utsumi R,Tanabe H,Nakamoto Y,Kawamukai M,Sakai H,Himeno M,Komano T,Hirota Y

更新日期：1981-09-01 00:00:00

abstract：:Acid hydrolysates of 140 strains, representing 11 species of the genus Bacteroides, were analyzed by capillary gas-liquid chromatography for total cellular fatty acid. All samples contained components which appeared to be hydroxy fatty acid. The relative amount and chain length distribution of the hydroxy fatty acids,...

journal_title：Journal of bacteriology

authors： Mayberry WR

更新日期：1980-08-01 00:00:00

abstract：:Bacteriophage N4 middle genes are transcribed by a phage-coded, heterodimeric, rifampin-resistant RNA polymerase, N4 RNA polymerase II (N4 RNAPII). Sequencing and transcriptional analysis revealed that the genes encoding the two subunits comprising N4 RNAPII are translated from a common transcript initiating at the N4...

journal_title：Journal of bacteriology

authors： Willis SH,Kazmierczak KM,Carter RH,Rothman-Denes LB

更新日期：2002-09-01 00:00:00

abstract：:We describe here a new mutant of Pseudomonas aeruginosa PAO, strain D10C (genotype plcB), which produces phospholipase C and alkaline phosphatase constitutively. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the extracellular proteins produced by this mutant in high- and low-Pi media revealed that the m...

journal_title：Journal of bacteriology

authors： Gray GL,Berka RM,Vasil ML

更新日期：1982-06-01 00:00:00

abstract：:Two primary alcohols (1-butanol and ethanol) are major fermentation products of several clostridial species. In addition to these two alcohols, the secondary alcohol 2-propanol is produced to a concentration of about 100 mM by some strains of Clostridium beijerinckii. An alcohol dehydrogenase (ADH) has been purified t...

journal_title：Journal of bacteriology

authors： Ismaiel AA,Zhu CX,Colby GD,Chen JS

更新日期：1993-08-01 00:00:00

abstract：:To understand further the role of the flagellum of Vibrio anguillarum in virulence, invasive and adhesive properties of isogenic motility mutants were analyzed by using a chinook salmon embryo cell line. Adhesion was unaffected but invasion of the cell line was significantly decreased in nonmotile or partially motile ...

journal_title：Journal of bacteriology

authors： Ormonde P,Hörstedt P,O'Toole R,Milton DL

更新日期：2000-04-01 00:00:00

abstract：:The activities of DNA methyltransferases are important for a variety of cellular functions in bacteria. In this study, we developed a modified high-throughput technique called methyl homopolymer tail mediated sequencing (methyl HTM-seq) to identify the undermethylated sites in the Vibrio cholerae genome for the two DN...

journal_title：Journal of bacteriology

authors： Dalia AB,Lazinski DW,Camilli A

更新日期：2013-05-01 00:00:00

abstract：:Corynebacterium diphtheriae, a Gram-positive, aerobic bacterium, is the causative agent of diphtheria and cutaneous infections. While mechanisms required for heme iron acquisition are well known in C. diphtheriae, systems involved in the acquisition of other metals such as zinc and manganese remain poorly characterize...

journal_title：Journal of bacteriology

authors： Peng ED,Oram DM,Battistel MD,Lyman LR,Freedberg DI,Schmitt MP

更新日期：2018-04-24 00:00:00

abstract：:Plasmid pP51 of Pseudomonas sp. strain P51 contains two gene clusters encoding the degradation of chlorinated benzenes, tcbAB and tcbCDEF. A regulatory gene, tcbR, was located upstream and divergently transcribed from the chlorocatechol oxidative gene cluster tcbCDEF. The tcbR gene was characterized by DNA sequencing ...

journal_title：Journal of bacteriology

authors： van der Meer JR,Frijters AC,Leveau JH,Eggen RI,Zehnder AJ,de Vos WM

更新日期：1991-06-01 00:00:00

abstract：:The DnaA protein concentration in Escherichia coli was increased above the wild-type level by inducing a lacP-controlled dnaA gene located on a plasmid. In these cells with different DnaA protein levels, we measured several parameters: dnaA gene expression; cell size, amount of DNA per cell, and number of origins per ...

journal_title：Journal of bacteriology

authors： Atlung T,Hansen FG

更新日期：1993-10-01 00:00:00

abstract：:Using a quantitative assay for measuring chemotaxis, we tested a variety of sugars and sugar derivatives for their ability to attract Escherichia coli bacteria. The most effective attractants, i.e., those that have thresholds near 10(-5) M or below, are N-acetyl-d-glucosamine, 6-deoxy-d-glucose, d-fructose, d-fucose, ...

journal_title：Journal of bacteriology

authors： Adler J,Hazelbauer GL,Dahl MM

更新日期：1973-09-01 00:00:00

abstract：:A detailed study of the cultural characteristics and cellular fatty acid composition of 27 isolates of Corynebacterium acnes was performed to establish the properties by which this organism may be identified and characterized. The fatty acids were extracted directly from whole cells and examined as methyl esters by ga...

journal_title：Journal of bacteriology

authors： Moss CW,Dowell VR Jr,Lewis VJ,Schekter MA

更新日期：1967-11-01 00:00:00

abstract：:Secretion vectors based on the genes from Bacillus amyloliquefaciens P for alkaline protease (aprBamP) and neutral protease (nprBamP) were constructed. With both aprBamP and nprBamP, a unique restriction site was introduced 3' of the predicted signal coding region by using the technique of oligonucleotide-directed mut...

journal_title：Journal of bacteriology

authors： Vasantha N,Thompson LD

更新日期：1986-03-01 00:00:00

abstract：:A procedure to identify genes that are lethal when cloned on multicopy plasmids was developed. It depends on the ability of mini-Mu plasmid elements to be used for both in vivo cloning and generalized transduction of enterobacterial genes. The feasibility of this procedure was demonstrated by using the tetA gene of Tn...

journal_title：Journal of bacteriology

authors： Berg CM,Liu L,Wang B,Wang MD

更新日期：1988-01-01 00:00:00

abstract：:Forty years ago, Coulter and Talalay (A. W. Coulter and P. Talalay, J. Biol. Chem. 243:3238-3247, 1968) established the oxygenase-dependent pathway for the degradation of testosterone by aerobes. The oxic testosterone catabolic pathway involves several oxygen-dependent reactions and is not available for anaerobes. Sin...

journal_title：Journal of bacteriology

authors： Leu YL,Wang PH,Shiao MS,Ismail W,Chiang YR

更新日期：2011-09-01 00:00:00

abstract：:Here we show that the type III secretion gatekeeper protein SepL resembles an aberrant effector protein in binding to a class 1 type III secretion chaperone (Orf12, here renamed CesL). We also show that short N-terminal fragments (≤70 amino acids) from SepL are capable of targeting fusion proteins for secretion and tr...

journal_title：Journal of bacteriology

authors： Younis R,Bingle LE,Rollauer S,Munera D,Busby SJ,Johnson S,Deane JE,Lea SM,Frankel G,Pallen MJ

更新日期：2010-11-01 00:00:00

abstract：:Germaine, G. R. (University of Minnesota, Minneapolis), and D. L. Anderson. Binding of homologous polymerized deoxyribonucleic acid by Streptomyces griseus. J. Bacteriol. 92:662-667. 1966-An irreversible P(32) deoxyribonucleic acid (DNA) binding system is described for Streptomyces griseus S104. The 69% guanine plus c...

journal_title：Journal of bacteriology

authors： Germaine GR,Anderson DL

更新日期：1966-09-01 00:00:00

abstract：:Listeria monocytogenes is a gram-positive, psychotrophic, food-borne pathogen which is able to grow in osmotically stressful environments. Carnitine (beta-hydroxy-L-tau-N-trimethyl aminobutyrate) can contribute significantly to growth of L. monocytogenes at high osmolarity (R. R. Beumer, M. C. te Giffel, L. J. Cox, F....

journal_title：Journal of bacteriology

authors： Verheul A,Rombouts FM,Beumer RR,Abee T

更新日期：1995-06-01 00:00:00

abstract：:An inorganic pyrophosphatase was purified over 600-fold to homogeneity as judged by polyacrylamide gel electrophoresis. The enzyme is a tetramer of Mr = 84,000, has a sedimentation coefficient of 5.8S, a Stokes radius of 3.5 nm, and an isoelectric point of 5.7. Like the enzyme of Escherichia coli, the pyrophosphatase ...

journal_title：Journal of bacteriology

authors： Verhoeven JA,Schenck KM,Meyer RR,Trela JM

更新日期：1986-10-01 00:00:00

abstract：:The GcvA protein is a LysR family regulatory protein necessary for both activation and repression of the Escherichia coli glycine cleavage enzyme operon (gcv) and negative regulation of gcvA. Gel shift assays indicated that overexpressed GcvA in crude extracts is capable of binding specifically to DNA containing the g...

journal_title：Journal of bacteriology

authors： Wilson RL,Urbanowski ML,Stauffer GV

更新日期：1995-09-01 00:00:00

abstract：:Grunberg, E. (Hoffman-La Roche, Inc., Nutley, N.J.), and R. Cleeland. Fluorescence and viability of Proteus mirabilis stained directly with fluorescein isothiocyanate. J. Bacteriol. 92:23-27. 1966.-Washed cell suspensions of Proteus mirabilis, under the proper conditions, stained well with fluorescein isothiocyanate w...

journal_title：Journal of bacteriology

authors： Grunberg E,Cleeland R

更新日期：1966-07-01 00:00:00