Purification and characterization of an inorganic pyrophosphatase from the extreme thermophile Thermus aquaticus.

abstract：:A delta tyrT::kan mutant from Escherichia coli K-12 (DTK-12) shows a transient growth lag that is caused by glycine starvation (U. Michelsen, M. Bösl, T. Dingermann, and H. Kersten, J. Bacteriol. 171:5987-5994, 1989). The same deletion, transduced into the relA1 spoT1 mutant CA274 to construct strain DTC274, caused co...

journal_title：Journal of bacteriology

authors： Bösl M,Kersten H

更新日期：1994-01-01 00:00:00

abstract：:The isolation of a new thermophilic bacterium, Thermus aquaticus gen. n. and sp. n., is described. Successful enrichment requires incubation at 70 to 75 C, and the use of nutrient media relatively dilute with respect to the organic components. Strains of T. aquaticus have been isolated from a variety of thermal spring...

journal_title：Journal of bacteriology

authors： Brock TD,Freeze H

更新日期：1969-04-01 00:00:00

abstract：:A new insertion sequence (IS element), IS1411, was identified downstream of the phenol degradation genes pheBA that originated from plasmid DNA of Pseudomonas sp. strain EST1001. According to sequence analysis, IS1411 belongs to a new family of IS elements that has recently been named the ISL3 family (J. Mahillon and ...

journal_title：Journal of bacteriology

authors： Kallastu A,Hõrak R,Kivisaar M

更新日期：1998-10-01 00:00:00

abstract：:Analysis of two virulence mutants of Pseudomonas syringae B728a revealed that the Tn 5 sites of insertion were within the gidA open reading frame (ORF). These mutations were pleiotropic, affecting diverse phenotypic traits, such as lipodepsipeptide (syringomycin and syringopeptin) antibiotic production, swarming, pres...

journal_title：Journal of bacteriology

authors： Kinscherf TG,Willis DK

更新日期：2002-04-01 00:00:00

abstract：:Escherichia coli AT2046 has been shown previously to lack the enzyme serine transhydroxymethylase and to require exogenous glycine for growth as a consequence. Strains JEV73 and JEV73R, mutants derived from strain AT2046, are shown here to be serine transhydroxymethylase deficient, but able to derive their glycine fro...

journal_title：Journal of bacteriology

authors： Fraser J,Newman EB

更新日期：1975-06-01 00:00:00

abstract：:Lipopolysaccharide (LPS) fractions were isolated from three species of blue-green algae of the genus Phormidium, namely, P. africanum, P. laminosum, and P. uncinatum, by using a phenol-water procedure followed by exhaustive extraction with ammonium oxalate. The materials obtained were shown to be closely related bioch...

journal_title：Journal of bacteriology

authors： Mikheyskaya LV,Ovodova RG,Ovodov YS

更新日期：1977-04-01 00:00:00

abstract：:Unique lipids found in Azotobacter vinelandii cysts are derived primarily from beta-hydroxybutyrate used to induce encystment. Tracer studies with beta-[14C]hydroxybutyrate showed that the biosynthesis of these compounds during encystment began at 8 to 12 h after induction and reached maximal levels after 2 days, Seve...

journal_title：Journal of bacteriology

authors： Su CJ,Sadoff HL

更新日期：1981-07-01 00:00:00

abstract：:The predominant mode of growth of bacteria in the environment is within sessile, matrix-enclosed communities known as biofilms. Biofilms often complicate chronic and difficult-to-treat infections by protecting bacteria from the immune system, decreasing antibiotic efficacy, and dispersing planktonic cells to distant b...

journal_title：Journal of bacteriology

authors： Jackson DW,Suzuki K,Oakford L,Simecka JW,Hart ME,Romeo T

更新日期：2002-01-01 00:00:00

abstract：:In rod-shaped bacteria, certain proteins are specifically localized to the cell poles. The nature of the positional information that leads to the proper localization of these proteins is unclear. In a screen for factors required for the localization of the Shigella sp. actin assembly protein IcsA to the bacterial pole...

journal_title：Journal of bacteriology

authors： Nilsen T,Yan AW,Gale G,Goldberg MB

更新日期：2005-09-01 00:00:00

abstract：:Growth of a derivative of Escherichia coli K-10 was strongly inhibited by 2 times 10(-4) M L-5(1-amino-2-methylbutyl)-tetrazole (isoleucine tetrazole). Growth inhibition was reversed by isoleucine, threonine, glycyl-L-isoleucine, or glycyl-L-threonine, and, in a valine-resistant mutant, by L-valine. Partial reversal o...

journal_title：Journal of bacteriology

authors： Willshaw GA,Tristram H

更新日期：1975-09-01 00:00:00

abstract：:Walls of Mortierella parvispora, Pullularia pullulans, Absidia repens, Fusarium oxysporum, and of several Penicillium species varied in their susceptibilities to digestion by glucanase and chitinase. Polysaccharides were present in the residues remaining after enzymatic digestion. Acid hydrolysates of the walls contai...

journal_title：Journal of bacteriology

authors： Pengra RM,Cole MA,Alexander M

更新日期：1969-03-01 00:00:00

abstract：:The structural gene for histidyl-tRNA synthetase was localized to 53.8 min on the Escherichia coli genome. The gene order in this region was determined to be dapE-purC-upp-purG-(guaA, guaB)-hisS-glyA. ...

journal_title：Journal of bacteriology

authors： Parker J,Fishman SE

更新日期：1979-04-01 00:00:00

abstract：:Plasmids encoding the structural genes for the Rhodobacter capsulatus and Rhodobacter sphaeroides cytochrome (cyt) bc1 complexes were introduced into strains of R. capsulatus lacking the cyt bc1 complex, with and without cyt c2. The R. capsulatus merodiploids contained higher than wild-type levels of cyt bc1 complex, ...

journal_title：Journal of bacteriology

authors： Davidson E,Prince RC,Haith CE,Daldal F

更新日期：1989-11-01 00:00:00

abstract：:The Escherichia coli purine repressor, PurR, binds to a 16-bp operator sequence and coregulates the genes for de novo synthesis of purine and pyrimidine nucleotides, formation of a one-carbon unit for biosynthesis, and deamination of cytosine. We have characterized the purified repressor. Chemical cross-linking indica...

journal_title：Journal of bacteriology

authors： Choi KY,Zalkin H

更新日期：1992-10-01 00:00:00

abstract：:Bacterial flagellar filaments grow at their distal ends, from flagellin that travels through a central channel ∼2 nm in diameter. The flagellin is extruded from the cytoplasm by a pump powered by a proton motive force (PMF). We measured filament growth in cells near the mid-exponential-phase with flagellin bearing a s...

journal_title：Journal of bacteriology

authors： Turner L,Stern AS,Berg HC

更新日期：2012-05-01 00:00:00

abstract：:The highly toxic oxyanion tellurite (TeO3(2-)) is a well known pro-oxidant in mammalian and bacterial cells. This work examines the effects of tellurite on the redox state of the electron transport chain of the facultative phototroph Rhodobacter capsulatus, in relation to the role of the thiol:disulfide oxidoreductase...

journal_title：Journal of bacteriology

authors： Borsetti F,Francia F,Turner RJ,Zannoni D

更新日期：2007-02-01 00:00:00

abstract：:Kimler, Alexander (National Institutes of Health, Bethesda, Md.). Fluorescein amine media for rapid differentiation of staphylococci. J. Bacteriol. 82:106-109. 1961.-By modifying conventional media to include blood, mannitol, and fluorescein amine, staphylococci may be isolated and identified directly. Staphylococcus ...

journal_title：Journal of bacteriology

authors： KIMLER A

更新日期：1961-07-01 00:00:00

abstract：:Salmonella enterica encodes a type III secretion system within a pathogenicity island located at centisome 63 that is essential for virulence. All type III secretion systems require the function of a family of low-molecular-weight proteins that aid the secretion process by acting as partitioning factors and/or secreti...

journal_title：Journal of bacteriology

authors： Tucker SC,Galán JE

更新日期：2000-04-01 00:00:00

abstract：:Mutations in carbohydrate-negative mutants of Pseudomonas aeruginosa PAO1 individually deficient in glucose 6-phosphate dehydrogenase (zwf), 6-phosphogluconate dehydratase (edd), or pyruvate carboxylase (pyc) were mapped on the chromosome by plasmid R68.45-mediated conjugation and by bacteriophage F116L-mediated trans...

journal_title：Journal of bacteriology

authors： Roehl RA,Feary TW,Phibbs PV Jr

更新日期：1983-12-01 00:00:00

abstract：:Niederpruem, Donald J. (Indiana University Medical Center, Indianapolis). Respiration of basidiospores of Schizophyllum commune. J. Bacteriol. 88:210-215. 1964.-The aerobic metabolism of basidiospores of the wood-rotting mushroom Schizophyllum commune was investigated by use of manometric techniques and specific respi...

journal_title：Journal of bacteriology

authors： NIEDERPRUEM DJ

更新日期：1964-07-01 00:00:00

abstract：:Klebsiella pneumoniae is a nosocomial pathogen frequently isolated from opportunistic infections, especially in clinical environments. In spite of its potential pathogenicity, this microorganism has several metabolic potentials that could be used in biotechnology applications. K. pneumoniae is able to metabolize glyce...

journal_title：Journal of bacteriology

authors： Marçal D,Rêgo AT,Carrondo MA,Enguita FJ

更新日期：2009-02-01 00:00:00

abstract：:We report here the structure of a previously uncharacterized phospholipid in the radiation-resistant bacterium Deinococcus radiodurans. This phospholipid, designated lipid 4, was shown by chemical analysis, HF hydrolysis, and nuclear magnetic resonance spectroscopy to be phosphatidylglyceroylalkylamine. Lipid 4 thus c...

journal_title：Journal of bacteriology

authors： Huang Y,Anderson R

更新日期：1991-01-01 00:00:00

abstract：:We identified in Streptococcus mutans six new genes (rgpA through rgpF), whose disruption results in a loss of serotype-specific antigenicity, specified by the glucose side chains of rhamnose-glucose polysaccharide from the cell wall. Rhamnose and glucose content of the cell wall decreased drastically in all these dis...

journal_title：Journal of bacteriology

authors： Yamashita Y,Tsukioka Y,Tomihisa K,Nakano Y,Koga T

更新日期：1998-11-01 00:00:00

abstract：:The genomic organization of the acidophilic chemolithoautotrophic bacterium Thiobacillus ferrooxidans ATCC 21834 has been studied by pulsed-field gel electrophoresis (PFGE). Analysis of its intact DNA, as well as the restriction patterns obtained with several endonucleases, allowed the characterization of one circular...

journal_title：Journal of bacteriology

authors： Irazabal N,Marín I,Amils R

更新日期：1997-03-01 00:00:00

abstract：:Stocks, Peter K. (Louisiana State University, Baton Rouge), and C. S. McCleskey. Identity of the pink-pigmented methanol-oxidizing bacteria as Vibrio extorquens. J. Bacteriol. 88:1065-1070. 1964.-Pink-pigmented bacteria isolated from enrichment cultures of methane oxidizers were found to possess similar morphological,...

journal_title：Journal of bacteriology

authors： STOCKS PK,MCCLESKEY CS

更新日期：1964-10-01 00:00:00

abstract：:The maltose regulon consists of three operons controlled by a positive regulatory gene, malT. Deletions of the gene crp were introduced into strains which carried a malT-lacZ hybrid gene. From the observed reduction in beta-galactosidase activity it was concluded that the expression of malT-lacZ, and therefore of malT...

journal_title：Journal of bacteriology

authors： Chapon C

更新日期：1982-05-01 00:00:00

abstract：:Proteus mirabilis swarming behavior is characterized by the development of concentric rings of growth that are formed as cyclic events of swarmer cell differentiation, swarming migration, and cellular differentiation are repeated during colony translocation across a surface. This cycle produces the bull's-eye colony o...

journal_title：Journal of bacteriology

authors： Belas R,Schneider R,Melch M

更新日期：1998-12-01 00:00:00

abstract：:Between 59 and 60 min on the Escherichia coli genetic map, there is a highly conserved sequence of 29 base pairs, containing an inverted repeat of seven base pairs that appears 14 times, 32 or 33 base pairs apart, downstream of the iap gene coding region. About 24 kilobase pairs downstream of the 14 repeats, a similar...

journal_title：Journal of bacteriology

authors： Nakata A,Amemura M,Makino K

更新日期：1989-06-01 00:00:00

abstract：:The genes that encode the two subunits of Bacillus subtilis phenylalanyl-tRNA synthetase were cloned from alpha lambda library of chromosomal B. subtilis DNA by specific complementation of a thermosensitive Escherichia coli pheS mutation. Both genes (we named them pheS and pheT, analogous to the corresponding genes of...

journal_title：Journal of bacteriology

authors： Brakhage AA,Putzer H,Shazand K,Röschenthaler RJ,Grunberg-Manago M

更新日期：1989-02-01 00:00:00

abstract：:Wolin, M. J. (University of Illinois, Urbana). Lysis of Vibrio succinogenes by ethylenediaminetetraacetic acid or lysozyme. J. Bacteriol. 91:1781-1786. 1966.-Cell suspensions of Vibrio succinogenes are lysed by ethylenediaminetetraacetic acid (EDTA) or lysozyme. Lysis occurs at alkaline pH and is prevented by 0.15 m N...

journal_title：Journal of bacteriology

authors： Wolin MJ

更新日期：1966-05-01 00:00:00