Phospholipase C regulatory mutation of Pseudomonas aeruginosa that results in constitutive synthesis of several phosphate-repressible proteins.

abstract：:Genes from Bacillus subtilis predicted to encode a phosphate-specific transport (Pst) system were shown by mutation to affect high-affinity Pi uptake but not arsenate resistance or phosphate (Pho) regulation. The transcription start of the promoter upstream of the pstS gene was defined by primer extension. The promote...

journal_title：Journal of bacteriology

authors： Qi Y,Kobayashi Y,Hulett FM

更新日期：1997-04-01 00:00:00

abstract：:Virulence genes in Listeria monocytogenes are coordinately expressed under the control of the transcriptional activator PrfA, encoded by prfA, a member of the cyclic AMP (cAMP) receptor protein (CRP)/FNR family of bacterial regulators. Strain P14-A is a spontaneous mutant of L. monocytogenes serovar 4b which produces ...

journal_title：Journal of bacteriology

authors： Ripio MT,Domínguez-Bernal G,Lara M,Suárez M,Vazquez-Boland JA

更新日期：1997-03-01 00:00:00

abstract：:Oxygen utilization in splenic cells of normal and BCG-sensitized guinea pigs was measured in a Warburg respirometer in the absence and in the presence of Old Tuberculin (OT). In the absence of OT, normal and sensitive cells respired at the same rate; in the presence of OT, normal cell respiration remained unchanged, w...

journal_title：Journal of bacteriology

authors： Kochan I,Khan A

更新日期：1969-01-01 00:00:00

abstract：:Examination of the ilvF locus at 54 min on the Escherichia coli K-12 chromosome revealed that it is a cryptic gene for expression of a valine-resistant acetohydroxy acid synthase (acetolactate synthase; EC 4.1.3.18) distinct from previously reported isozymes. A spontaneous mutation, ilvF663, yielded IlvF+ enzyme activ...

journal_title：Journal of bacteriology

authors： Alexander-Caudle C,Latinwo LM,Jackson JH

更新日期：1990-06-01 00:00:00

abstract：:An exo-beta-1,4-glucanase (Exo A) from Ruminococcus flavefaciens FD-1 was purified to homogeneity and characterized. Enzyme activity was monitored during purification by using the substrate p-nitrophenyl-beta-D-cellobioside (NPC). Over 85% of the NPC activity was found to be extracellular once the filter paper was deg...

journal_title：Journal of bacteriology

authors： Gardner RM,Doerner KC,White BA

更新日期：1987-10-01 00:00:00

abstract：:This article contains an analysis of a version of the well-known inhibitor-dilution model for the control of initiation of chromosome replication in bacteria. According to this model, an unstable inhibitor interacts with an initiation primer in a hit-and-destroy fashion to prevent successful initiation; both constitue...

journal_title：Journal of bacteriology

authors： Margalit H,Grover NB

更新日期：1987-11-01 00:00:00

abstract：:The genes encoding the two isoenzymes of methyl coenzyme M reductase (MRI and MRII) in Methanobacterium thermoautotrophicum delta H have been cloned and sequenced. The MRI-encoding mcr operon (mcrBDCGA) has been located immediately upstream from the mtr operon (mtrEDCBA) that encodes N5-methyltetrahydromethanopterin:c...

journal_title：Journal of bacteriology

authors： Pihl TD,Sharma S,Reeve JN

更新日期：1994-10-01 00:00:00

abstract：:A major phenotypic trait of the Mycobacterium avium complex is the ability to produce rough and smooth colony variants. The chemical basis of this morphological variation is the loss of an antigenic surface structure, termed glycopeptidolipid (GPL), by rough variants. Using M. avium serovar 2 strain 2151 as a model sy...

journal_title：Journal of bacteriology

authors： Eckstein TM,Inamine JM,Lambert ML,Belisle JT

更新日期：2000-11-01 00:00:00

abstract：:Grixazone (GX), which is a diffusible yellow pigment containing a phenoxazinone chromophore, is one of the secondary metabolites under the control of A-factor (2-isocapryloyl-3R-hydroxymethyl-gamma-butyrolactone) in Streptomyces griseus. GX production is also induced by phosphate starvation. The whole biosynthesis gen...

journal_title：Journal of bacteriology

authors： Higashi T,Iwasaki Y,Ohnishi Y,Horinouchi S

更新日期：2007-05-01 00:00:00

abstract：:We have isolated the gene encoding L-allo-threonine aldolase (L-allo-TA) from Aeromonas jandaei DK-39, a pyridoxal 5'-phosphate (PLP)-dependent enzyme that stereospecifically catalyzes the interconversion of L-allo-threonine and glycine. The gene contains an open reading frame consisting of 1,014 nucleotides correspon...

journal_title：Journal of bacteriology

authors： Liu JQ,Dairi T,Kataoka M,Shimizu S,Yamada H

更新日期：1997-06-01 00:00:00

abstract：:The cheW gene from Escherichia coli has been cloned an inducible promoter, and the effects of the overproduction of the CheW protein on chemotactic behavior and receptor covalent modification have been examined. Plasmids that contain the cheW gene behind a regulatable promoter complement a cheW mutation when the CheW ...

journal_title：Journal of bacteriology

authors： Sanders DA,Mendez B,Koshland DE Jr

更新日期：1989-11-01 00:00:00

abstract：:A monoclonal antibody, AFRC MAC 203, was used to examine the expression of a nodule-induced cell surface antigen associated with lipopolysaccharide in Rhizobium leguminosarum bv. viciae 3841. Silver-enhanced immunogold-labeled tissue sections revealed that, in very young tissues of pea root nodules, the nodule-induced...

journal_title：Journal of bacteriology

authors： VandenBosch KA,Brewin NJ,Kannenberg EL

更新日期：1989-09-01 00:00:00

abstract：:Nucleotide excision repair (NER) is universally used to recognize and remove many types of DNA damage. In eubacteria, the NER system typically consists of UvrA, UvrB, UvrC, the UvrD helicase, DNA polymerase I, and ligase. In addition, when DNA damage blocks transcription, transcription-repair coupling factor (TRCF), t...

journal_title：Journal of bacteriology

authors： LeCuyer BE,Criss AK,Seifert HS

更新日期：2010-02-01 00:00:00

abstract：:A spontaneous lac+ revertant of an adenylate cyclase deletion strain of Escherichia coli K-12 was isolated and characterized. This revertant, designated strain KC20, exhibited a pleiotropic suppression of the adenylate cyclase defect, with the crp locus being the site of the suppressor mutation. Cyclic adenosine 3',5'...

journal_title：Journal of bacteriology

authors： Bankaitis VA,Kline EL

更新日期：1981-08-01 00:00:00

abstract：:Evidence of the presence of messenger ribonucleic acid (mRNA) in dormant spores of Bacillus subtilis has been obtained. The bulk RNA from spores was isolated and labeled in vitro with tritiated dimethyl sulfate. The spore RNA hybridized to 2.4 to 3.2% of the B. subtilis genome. The RNA hybridized to both the complemen...

journal_title：Journal of bacteriology

authors： Jeng YH,Doi RH

更新日期：1974-08-01 00:00:00

abstract：:phiYeO3-12 is a T3-related lytic bacteriophage of Yersinia enterocolitica serotype O:3. The nucleotide sequence of the 39,600-bp linear double-stranded DNA (dsDNA) genome was determined. The phage genome has direct terminal repeats of 232 bp, a GC content of 50.6%, and 54 putative genes, which are all transcribed from...

journal_title：Journal of bacteriology

authors： Pajunen MI,Kiljunen SJ,Söderholm ME,Skurnik M

更新日期：2001-03-01 00:00:00

abstract：:Caulobacter crescentus initiates a single round of DNA replication during each cell cycle. Following the initiation of DNA replication, the essential CckA histidine kinase is activated by phosphorylation, which (via the ChpT phosphotransferase) enables the phosphorylation and activation of the CtrA global regulator. C...

journal_title：Journal of bacteriology

authors： Iniesta AA,Hillson NJ,Shapiro L

更新日期：2010-08-01 00:00:00

abstract：:The effect of motility on the competitive success of Rhizobium meliloti in nodule production was investigated. A motile strain formed more nodules than expected when mixed at various unfavorable ratios with either flagellated or nonflagellated nonmotile derivatives. We conclude that motility confers a selective advant...

journal_title：Journal of bacteriology

authors： Ames P,Bergman K

更新日期：1981-11-01 00:00:00

abstract：:We report here the identification of a new lipoprotein, NlpI, in Escherichia coli K-12. The NlpI structural gene (nlpI) is located between the genes pnp (polynucleotide phosphorylase) and deaD (RNA helicase) at 71 min on the E. coli chromosome. The nlpI gene encodes a putative polypeptide of approximately 34 kDa, and ...

journal_title：Journal of bacteriology

authors： Ohara M,Wu HC,Sankaran K,Rick PD

更新日期：1999-07-01 00:00:00

abstract：:A pLAFR3 cosmid clone designated pVir2 containing a 25-kilobase (kb) DNA insert was isolated from a wild-type Pseudomonas solanacearum GMI1000 genomic library. This cosmid was shown to complement all but one of the nine Tn5-induced mutants which have been isolated after random mutagenesis and which have lost both path...

journal_title：Journal of bacteriology

authors： Boucher CA,Van Gijsegem F,Barberis PA,Arlat M,Zischek C

更新日期：1987-12-01 00:00:00

abstract：:The vitamin requirements of the group Q streptococci (Streptococcus avium) and the established enterococcal species (S. faecalis and S. faecium) paralleled one another, although S. avium did not characteristically require riboflavine or pyridoxal. S. avium was further differentiated in that it required thymine for gro...

journal_title：Journal of bacteriology

authors： Nowlan SS,Deibel RH

更新日期：1967-08-01 00:00:00

abstract：:The Rhizobium phaseoli recA gene has been cloned by interspecific complementation of the Fec phenotype of bacteriophage lambda. The cloned gene restored the recombination proficiency and conferred resistance to DNA-damaging agents (methyl methanesulfonate and nitrofurantoin) to an Escherichia coli recA mutant. The dir...

journal_title：Journal of bacteriology

authors： Martínez-Salazar JM,Romero D,Girard ML,Dávila G

更新日期：1991-05-01 00:00:00

abstract：:Natural isolates of pathogenic bacteria can exhibit a broad range of phenotypic traits. To investigate the molecular mechanisms contributing to such phenotypic variability, we compared the genomes, transcriptomes, and proteomes of two natural isolates of the gram-negative bacterium Burkholderia pseudomallei, the causa...

journal_title：Journal of bacteriology

authors： Ou K,Ong C,Koh SY,Rodrigues F,Sim SH,Wong D,Ooi CH,Ng KC,Jikuya H,Yau CC,Soon SY,Kesuma D,Lee MA,Tan P

更新日期：2005-06-01 00:00:00

abstract：:A mutant of Escherichia coli lacking hydroxyethylthiazole kinase (EC 2.7.1.50) was produced by a further mutation of a temperature-sensitive, auxotrophic mutant for hydroxyethylthiazole. The parent cells possessed two distinct enzymes capable of phosphorylating hydroxyethylthiazole: one was hydroxyethylthiazole kinase...

journal_title：Journal of bacteriology

authors： Mizote T,Nakayama H

更新日期：1989-06-01 00:00:00

abstract：:Bacterial flagellar filaments grow at their distal ends, from flagellin that travels through a central channel ∼2 nm in diameter. The flagellin is extruded from the cytoplasm by a pump powered by a proton motive force (PMF). We measured filament growth in cells near the mid-exponential-phase with flagellin bearing a s...

journal_title：Journal of bacteriology

authors： Turner L,Stern AS,Berg HC

更新日期：2012-05-01 00:00:00

abstract：:Pathways of ammonia assimilation into glutamic acid in Bacillus macerans were investigated by measurements of the specific activities of glutamate dehydrogenase (GDH), glutamine synthetase, and glutamate synthase. In ammonia-rich medium, GDH was the predominant pathway of ammonia assimilation. In nitrogen-fixing cells...

journal_title：Journal of bacteriology

authors： Kanamori K,Weiss RL,Roberts JD

更新日期：1987-10-01 00:00:00

abstract：:Analysis of strains with deletions of all or part of nif have ordered the Klebsiella pneumoniae genetic loci as thi rbt dal udk gnd rfb has nif shiA. The his-nif plasmids pRD1 and pTM4010 contain the genes gnd rfb his nif shiA. ...

journal_title：Journal of bacteriology

authors： MacNeil D,Supiano MA,Brill WJ

更新日期：1979-06-01 00:00:00

abstract：:Activation of the late prespore-specific RNA polymerase sigma factor sigma(G) during Bacillus subtilis sporulation coincides with completion of the engulfment process, when the prespore becomes a protoplast fully surrounded by the mother cell cytoplasm and separated from it by a double membrane system. Activation of s...

journal_title：Journal of bacteriology

authors： Serrano M,Vieira F,Moran CP Jr,Henriques AO

更新日期：2008-12-01 00:00:00

abstract：:Recombination studies of bacteria are often carried out with multicopy plasmids, and recombination frequencies are often deduced from the proportion of cells in the population that express a recombinant phenotype. These frequencies should however be called apparent frequencies, since detection of the recombinant cells...

journal_title：Journal of bacteriology

authors： Chédin F,Dervyn R,Ehrlich SD,Noirot P

更新日期：1997-02-01 00:00:00

abstract：:The orientation of lipoteichoic acid (LTA) molecules on the surface of bacterial cells undoubtedly is determined by the ability of the LTA, during its transit through the cell wall, to bind via its polyglycerophosphate backbone or its glycolipid moieties to other constituents of the cytoplasmic membrane and the cell w...

journal_title：Journal of bacteriology

authors： Ofek I,Simpson WA,Beachey EH

更新日期：1982-02-01 00:00:00