Binding of homologous polymerized deoxyribonucleic acid by Streptomyces griseus.

Abstract:

:Germaine, G. R. (University of Minnesota, Minneapolis), and D. L. Anderson. Binding of homologous polymerized deoxyribonucleic acid by Streptomyces griseus. J. Bacteriol. 92:662-667. 1966-An irreversible P(32) deoxyribonucleic acid (DNA) binding system is described for Streptomyces griseus S104. The 69% guanine plus cytosine (GC) S. griseus DNA was bound by late exponential -early stationary phase cultures. Simultaneous addition of deoxyribonuclease with the P(32)-DNA to maximally receptive cultures reduced the uptake by 98%. Saturation of the binding ability occurred after 20-min incubation of cells with P(32)-DNA. Preparative cesium chloride density gradient centrifugation of the P(32)-DNA revealed that 98% of the label banded in the position expected for S. griseus DNA. Sedimentation of the P(32)-DNA at both 23 and 37 mug/ml indicated a molecular weight of 10.8 million. Attempts to transform S. griseus S104 auxotrophs to prototrophy by use of immediate and delayed selective procedures were unsuccessful.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Germaine GR,Anderson DL

doi

10.1128/JB.92.3.662-667.1966

subject

Has Abstract

pub_date

1966-09-01 00:00:00

pages

662-7

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

92

pub_type

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