Abstract:
:Dworkin, Martin (University of Minnesota, Minneapolis), and Donald J. Niederpruem. Electron transport system in vegetative cells and microcysts of Myxococcus xanthus. J. Bacteriol. 87:316-322. 1964.-Respiration by intact cells of the fruiting myxobacterium Myxococcus xanthus is cyanide-sensitive and can be demonstrated in the vegetative cells but not in the microcysts. Cell-free particles from both vegetative cells and microcysts have cyanide-sensitive reduced nicotinamide adenine dinucleotide (NADH) oxidase, diaphorase, NADH cytochrome c reductase, and cytochrome oxidase activities. While the vegetative cell specific activities for NADH oxidase and diaphorase are slightly higher than those for the microcysts, the microcysts have ten times the cytochrome c reductase and cytochrome oxidase activities of the vegetative cells. Furthermore, the respiration of the microcyst particles is considerably less cyanide-sensitive than is that of the vegetative-cell particles. Difference spectra of the cell-free particles of vegetative cells and microcysts are qualitatively identical, showing the presence of b- and c-type cytochrome and flavoprotein. The a-type pigments are clearly present in the extracts of the vegetative cells and are suggested by the spectrum of the microcyst particles. The cytochrome oxidase activity of both extracts is consistent with the presence of a-type pigments in both. The spectra of the carbon monoxide-binding pigments were determined and, by this parameter, qualitative differences appear between the vegetative cells and the microcysts.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
DWORKIN M,NIEDERPRUEM DJdoi
10.1128/JB.87.2.316-322.1964keywords:
subject
Has Abstractpub_date
1964-02-01 00:00:00pages
316-22eissn
0021-9193issn
1098-5530journal_volume
87pub_type
杂志文章abstract::Developmental expression of the Myxococcus xanthus gene 4521 requires extracellular A-signal. This signal is generated in response to nutrient limitation and functions in cell density sensing. To identify the upstream limit of the minimum region required in vivo for A-signal-dependent 4521 expression, a 5' deletion an...
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更新日期:1973-02-01 00:00:00
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更新日期:1973-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.114.3.951-955.1973
更新日期:1973-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1988-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.2.279-283.1963
更新日期:1963-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.1.61-65.1967
更新日期:1967-07-01 00:00:00
abstract::The asg mutants of Myxococcus xanthus are defective in production of extracellular A-signal, which serves as a cell density signal for fruiting-body development. The DNA sequence of asgB, one of the three asg genes, was determined. The deduced amino acid sequence of AsgB contains a DNA-binding helix-turn-helix motif n...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.7.2013-2020.1994
更新日期:1994-04-01 00:00:00