Abstract:
:One step in de novo pyridoxine (vitamin B6) and pyridoxal 5'-phosphate biosynthesis was predicted to be an oxidation catalyzed by an unidentified D-erythrose-4-phosphate dehydrogenase (E4PDH). To help identify this E4PDH, we purified the Escherichia coli K-12 gapA- and gapB-encoded dehydrogenases to homogeneity and tested whether either uses D-erythrose-4-phosphate (E4P) as a substrate. gapA (gap1) encodes the major D-glyceraldehyde-3-phosphate dehydrogenase (GA3PDH). The function of gapB (gap2) is unknown, although it was suggested that gapB encodes a second form of GA3PDH or is a cryptic gene. We found that the gapB-encoded enzyme is indeed an E4PDH and not a second GA3PDH, whereas gapA-encoded GA3PDH used E4P poorly, if at all, as a substrate under the in vitro reaction conditions used in this study. The amino terminus of purified E4PDH matched the sequence predicted from the gapB DNA sequence. Purified E4PDH was a heat-stable tetramer with a native molecular mass of 132 kDa. E4PDH had an apparent Km value for E4P [Kmapp(E4P)] of 0.96 mM, an apparent kcat catalytic constant for E4P [kcatapp(E4P)] of 200 s-1, Kmapp(NAD+) of 0.074 mM, and kcatapp(NAD+) of 169 s-1 in steady-state reactions in which NADH formation was determined. From specific activities in crude extracts, we estimated that there are at least 940 E4PDH tetramer molecules per bacterium growing in minimal salts medium plus glucose at 37 degrees C. Thin-layer chromatography confirmed that the product of the E4PDH reaction was likely the aldonic acid 4-phosphoerythronate. To establish a possible role of E4PDH in pyridoxal 5'-phosphate biosynthesis, we showed that 4-phosphoerythronate is a likely substrate for the 2-hydroxy-acid dehydrogenase encoded by the pdxB gene. Implications of these findings in the evolution of GA3PDHs are also discussed. On the basis of these results, we propose renaming gapB as epd (for D-erythrose-4-phosphate dehydrogenase).
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Zhao G,Pease AJ,Bharani N,Winkler MEdoi
10.1128/jb.177.10.2804-2812.1995subject
Has Abstractpub_date
1995-05-01 00:00:00pages
2804-12issue
10eissn
0021-9193issn
1098-5530journal_volume
177pub_type
杂志文章abstract::The chemical composition of the cell walls of several L-form revertants derived from Nocardia asteroides 10905 was determined at different stages of growth. It was observed that each L-form revertant had a cell well that differed from that of the parental strain when grown under identical conditions. In some strains t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.148.2.600-609.1981
更新日期:1981-11-01 00:00:00
abstract::The Escherichia coli K-12 mtr gene, which encodes a tryptophan-specific permease, was cloned, and its nucleotide sequence was determined. The precise location of the mtr gene at 69 min on the E. coli chromosome was determined. The mtr gene product was identified as a 414-amino-acid residue protein with a calculated mo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.13.4133-4143.1991
更新日期:1991-07-01 00:00:00
abstract::The parDE operon, located within the 3.2-kb stabilization region of plasmid RK2, encodes antitoxin (ParD) and toxin (ParE) proteins that stabilize the maintenance of this broad-host-range plasmid via a postsegregational killing mechanism. A ParE protein derivative, designated ParE', was purified by construction of a f...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.5.1420-1429.1996
更新日期:1996-03-01 00:00:00
abstract::Pseudomonas putida DOT-T1 was isolated after enrichment on minimal medium with 1% (vol/vol) toluene as the sole C source. The strain was able to grow in the presence of 90% (vol/vol) toluene and was tolerant to organic solvents whose log P(ow) (octanol/water partition coefficient) was higher than 2.3. Solvent toleranc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.14.3911-3916.1995
更新日期:1995-07-01 00:00:00
abstract::In vivo assembly of the Klebsiella aerogenes urease nickel metallocenter requires the presence of UreD, UreF, and UreG accessory proteins and is further facilitated by UreE. Prior studies had shown that urease apoprotein exists in an uncomplexed form as well as in a series of UreD-urease (I.-S. Park, M.B. Carr, and R....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.18.5417-5421.1996
更新日期:1996-09-01 00:00:00
abstract::The conversion of Pseudomonas aeruginosa PAO to the mucoid phenotype has been reported for a chronic pulmonary infection model in rats (D. E. Woods, P. A. Sokol, L. E. Bryan, D. G. Storey, S. J. Mattingly, H. J. Vogel, and H. Ceri, J. Infect. Dis. 163:143-149, 1991). This conversion was associated with a genetic rearr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.3.553-562.1994
更新日期:1994-02-01 00:00:00
abstract::Unsaturated fatty acid (ufa) auxotrophs of Neurospora crassa were obtained by treatment of conidia with N-methyl-N'-nitro-N-nitrosoguanidine followed by isolation on media containing polyunsaturated fatty acids suspended in Tergitol NP-40. The 24 mutants for which reisolates were obtained from crosses with wild type w...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.130.3.1144-1148.1977
更新日期:1977-06-01 00:00:00
abstract::Alginate-producing (mucoid) strains of Pseudomonas aeruginosa possess a 54-kDa outer membrane (OM) protein (AlgE) which is missing in nonmucoid bacteria. The coding region of the algE gene from mucoid P. aeruginosa CF3/M1 was subcloned in the expression vector pT7-7 and expressed in Escherichia coli. The level of expr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.18.5639-5647.1994
更新日期:1994-09-01 00:00:00
abstract::Human dental biofilm communities comprise several species, which can interact cooperatively or competitively. Bacterial interactions influence biofilm formation, metabolic changes, and physiological function of the community. Lactic acid, a common metabolite of oral bacteria, was measured in the flow cell effluent of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01631-09
更新日期:2010-06-01 00:00:00
abstract::Orf20 of the conjugative transposon Tn916 was purified as a chimeric protein fused to maltose binding protein (MBP-Orf20). The chimeric protein possessed endonucleolytic activity, cleaving both strands of the Tn916 origin of conjugal transfer (oriT) at several distinct sites and favoring GT dinucleotides. Incubation o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.6.2207-2213.2006
更新日期:2006-03-01 00:00:00
abstract::Mutants for 9 of the 10 steps in histidine biosynthesis have been isolated and identified by enzyme assay. Each locus has been mapped in relation to the aro cluster and to other histidine loci by deoxyribonucleic acid-mediated transformation. The genes which code for enzymes 3, 6, and 8 of the pathway are linked to th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.3.1444-1448.1969
更新日期:1969-03-01 00:00:00
abstract::The WO-1 strain of Candida albicans is capable of alternating between two highly distinct yeast cell types termed white and opaque (E. H. A. Rikkerrink, B. B. Magee, and P. T. Magee, J. Bacteriol. 170:895-899, 1988; B. Slutsky, M. Staebell, J. Anderson, L. Risen, M. Pfaller, and D. R. Soll, J. Bacteriol. 169:189-197, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.23.7436-7442.1991
更新日期:1991-12-01 00:00:00
abstract::We report the draft genome of the strain Lactobacillus gigeriorum CRBIP 24.85(T), isolated from a chicken crop. The total length of the 60 scaffolds is about 1.9 Mb, with a GC content of 38% and 2,062 protein-coding sequences (CDS). ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01427-12
更新日期:2012-11-01 00:00:00
abstract::The xylB and xylE genes in the TOL plasmid of Pseudomonas putida mt-2, which code for benzyl alcohol dehydrogenase and catechol 2,3-oxygenase, respectively, were cloned onto plasmid pBR322 in Escherichia coli for detailed mapping. The xylB gene was mapped in a 2.9-kilobase region within the BamHI BC fragment of pTN2, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.145.3.1137-1143.1981
更新日期:1981-03-01 00:00:00
abstract:UNLABELLED:Mycobacterium tuberculosis possesses a thick and highly hydrophobic cell wall principally composed of a mycolyl-arabinogalactan-peptidoglycan complex, which is critical for survival and virulence. DprE1 is a well-characterized component of decaprenyl-phospho-ribose epimerase, which produces decaprenyl-phosph...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00628-15
更新日期:2015-12-01 00:00:00
abstract::In Escherichia coli the SOS response, induced by DNA-damaging treatments, includes two systems of cell division inhibition, SfiA and SfiC, which are thought to prevent cell division by interacting with the division protein FtsZ. It is shown here that SfiA-mediated division inhibition is readily reversible, even in the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.166.3.733-738.1986
更新日期:1986-06-01 00:00:00
abstract::The alpha-aminoadipate pathway for lysine biosynthesis is present only in fungi. The alpha-aminoadipate reductase (AAR) of this pathway catalyzes the conversion of alpha-aminoadipic acid to alpha-aminoadipic-delta-semialdehyde by a complex mechanism involving two gene products, Lys2p and Lys5p. The LYS2 and LYS5 genes...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.24.7120-7125.2001
更新日期:2001-12-01 00:00:00
abstract::Escherichia coli ssrA encodes a small stable RNA molecule, tmRNA, that has many diverse functions, including tagging abnormal proteins for degradation, supporting phage growth, and modulating the activity of DNA binding proteins. Here we show that ssrA plays a role in Salmonella enterica serovar Typhimurium pathogenes...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.6.1558-1563.2000
更新日期:2000-03-01 00:00:00
abstract::DNA sequence analysis of the modABCD operon of Escherichia coli revealed the presence of four open reading frames. The first gene, modA, codes for a 257-amino-acid periplasmic binding protein enunciated by the presence of a signal peptide-like sequence. The second gene (modB) encodes a 229-amino-acid protein with a po...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.17.4851-4856.1995
更新日期:1995-09-01 00:00:00
abstract::Staphylococcus aureus was shown to transport iron complexed to a variety of hydroxamate type siderophores, including ferrichrome, aerobactin, and desferrioxamine. An S. aureus mutant defective in the ability to transport ferric hydroxamate complexes was isolated from a Tn917-LTV1 transposon insertion library after sel...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.16.4394-4400.2000
更新日期:2000-08-01 00:00:00
abstract::Pertussis toxin (PT) is secreted from Bordetella pertussis by a type IV secretion system, known as the Ptl transporter, that comprises nine different proteins, PtlA to PtlI. In this study, we found that PtlD is required for the stability of three Ptl proteins, PtlE, PtlF, and PtlH. A region limited to the C-terminal 7...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01106-08
更新日期:2008-11-01 00:00:00
abstract::Type IIb heat-labile enterotoxin (LT-IIb) is produced by Escherichia coli 41. Restriction fragments of total cell DNA from strain 41 were cloned into a cosmid vector, and one cosmid clone that encoded LT-IIb was identified. The genes for LT-IIb were subcloned into a variety of plasmids, expressed in minicells, sequenc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.9.4945-4952.1989
更新日期:1989-09-01 00:00:00
abstract::Undomesticated strains of Bacillus subtilis exhibit extensive colony spreading on certain soft agarose media: first the formation of dendritic clusters of cells, followed by spreading (pellicle-like) growth to cover the entire surface. These phases of colonization are dependent on the level of potassium ion (K(+)) but...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.24.8462-8469.2005
更新日期:2005-12-01 00:00:00
abstract::The transforming activity of fractionated complementary strands of Diplococcus pneumoniae deoxyribonucleic acid (DNA) bands at the position of fully denatured DNA in CsCl at pH 11.0, and is completely (> 99.8%) destroyed by digestion with exonuclease-I. These results prove that pure single strands transform the normal...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.101.2.361-364.1970
更新日期:1970-02-01 00:00:00
abstract::Escherichia coli K-12 strain CU1008 cannot use L-serine as the sole carbon source, but it could use L-serine as an auxiliary carbon source with glucose, L-alanine, or pyruvate and could derive energy from L-serine to support oxygen uptake. CU1008 grew with L-serine if it was also provided with glycine and leucine. The...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.2.777-782.1982
更新日期:1982-08-01 00:00:00
abstract:UNLABELLED:Current knowledge regarding the mechanism that governs flagellar motor rotation in response to environmental stimuli stems mainly from the study of monotrichous and peritrichous bacteria. Little is known about how two polar flagella, one at each cell pole of the so-called amphitrichous bacterium, are coordin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00172-15
更新日期:2015-10-01 00:00:00
abstract::The discovery of a novel multicistronic operon that encodes phosphofructokinase, pyruvate kinase, and lactate dehydrogenase in the lactic acid bacterium Lactococcus lactis is reported. The three genes in the operon, designated pfk, pyk, and ldh, contain 340, 502, and 325 codons, respectively. The intergenic distances ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.9.2541-2551.1993
更新日期:1993-05-01 00:00:00
abstract::A deoxyribonucleic acid-directed in vitro system for the synthesis of Escherichia coli carbamoylphosphate synthase has been developed, and its properties have been studied. The system uses the deoxyribonucleic acid of a lambda phage carrying the car genes (lambdadcarAB) as template and mediates the synthesis of both s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.141.1.58-66.1980
更新日期:1980-01-01 00:00:00
abstract::We showed previously that the outer membrane of the Escherichia coli cell envelope normally contains about 200 to 250 B12 receptors, and that these receptors function both in B12 transport and as receptors for the E colicins. This paper shows that this receptor system is also shared with bacteriophage BF23. A strong p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.3.1032-1039.1976
更新日期:1976-03-01 00:00:00
abstract::Escherichia coli outer membranes were prepared by centrifugation to equilibrium in sucrose gradients and then treated with Sarkosyl in the presence of ethylenediaminetetraacetate. The polypeptide profiles of the two outer membrane preparations were compared by two-dimensional polyacrylamide gel electrophoresis. The pa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.144.1.425-427.1980
更新日期:1980-10-01 00:00:00