Competent Diplococcus pneumoniae accept both single- and double-stranded deoxyribonucleic acid.

Abstract:

:The transforming activity of fractionated complementary strands of Diplococcus pneumoniae deoxyribonucleic acid (DNA) bands at the position of fully denatured DNA in CsCl at pH 11.0, and is completely (> 99.8%) destroyed by digestion with exonuclease-I. These results prove that pure single strands transform the normally prepared competent cells of this species. Their efficiency is about 0.5% that of native DNA of comparable size.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Miao R,Guild WR

doi

10.1128/JB.101.2.361-364.1970

subject

Has Abstract

pub_date

1970-02-01 00:00:00

pages

361-4

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

101

pub_type

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