Abstract:
:Mutants of Escherichia coli K-12 unable to excise pyrimidine dimers from their deoxyribonucleic acid (DNA) because of a uvr mutation show a higher survival when plated on a minimal salts medium after exposure to ultraviolet radiation than when plated on a complex medium such as nutrient agar containing yeast extract. This response has been called minimal medium recovery (MMR). Recovery of uvr mutants can take place in liquid as well as on solid medium, but not in buffer or under conditions of amino acid starvation that do not permit cell growth and normal DNA replication. MMR can thus be distinguished from the recovery of recombination-deficient (rec(-)uvr(+)) derivatives of K-12 which can occur under conditions where growth is not possible. Because MMR is characteristic of excision-defective mutants, it evidently reflects a type of repair independent of excision. We have obtained genetic evidence that MMR is determined by the rec genes, which also control recombination in K-12. Cells carrying a uvr mutation together with recA13, recA56, recB21, or recC22 failed to show MMR and were more sensitive to ultraviolet radiation than either their rec(+)uvr(-) or rec(-)uvr(+) parents. The rec(+)uvr(-) derivatives obtained from recA uvr(-) strains by transduction or by reversion regained the capacity for MMR. Our results indicate that inactivation of any one of the three genes, recA, recB, or recC, prevents cells from showing MMR.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Ganesan AK,Smith KCdoi
10.1128/JB.102.2.404-410.1970subject
Has Abstractpub_date
1970-05-01 00:00:00pages
404-10issue
2eissn
0021-9193issn
1098-5530journal_volume
102pub_type
杂志文章abstract::Tryptophan is an essential amino acid that is required for normal development in Chlamydia species, and tryptophan metabolism has been implicated in chlamydial persistence and tissue tropism. The ability to synthesize tryptophan is not universal among the Chlamydiaceae, but species that have a predicted tryptophan bio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01660-05
更新日期:2006-06-01 00:00:00
abstract::The ethanol oxidase respiratory chain of Gluconobacter suboxydan was characterized by using G. suboxydans subsp. alpha, a variant species of G. suboxydans incapable of oxidizing ethanol. The membranes of G. suboxydans subsp. alpha exhibited neither alcohol dehydrogenase, ethanol oxidase, nor glucose-ferricyanide oxido...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.11.3440-3445.1991
更新日期:1991-06-01 00:00:00
abstract::The electron transport system of Acinetobacter sp. HO1-N was studied to determine the specific cytochromes and to measure changes in the composition of the respiratory system due to growth in various concentrations of oxygen or types of growth substrates. Spectrophotometric analysis revealed that the quantity and type...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.142.3.859-868.1980
更新日期:1980-06-01 00:00:00
abstract::This is the first study of sporogenesis in Streptomyces carried out on a relatively high number of species (seven) which allows us, using also previously published results, to establish a general picture of this process. In the sporogenesis of Streptomyces two basic stages can be considered: the sporulation septum syn...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.127.3.1443-1454.1976
更新日期:1976-09-01 00:00:00
abstract::When 100 group A streptococcus isolates were screened, drs, a variant of sic, was identified in emm12 and emm55 isolates. Molecular characterization showed that the drs gene sequence is highly conserved, unlike the sic gene sequence. However, the variation in gene size observed was due to the presence of extra interna...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01619-06
更新日期:2007-04-01 00:00:00
abstract::A series of intercellular signals are involved in the regulation of gene expression during fruiting body formation of Myxococcus xanthus. Mutations which block cell interactions, such as csgA (formerly known as spoC), also prevent expression of certain developmentally regulated promoters. csgA+ cells containing Tn5 la...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.12.5552-5556.1988
更新日期:1988-12-01 00:00:00
abstract::The Brevibacterium acetylicum gsk gene, which encodes guanosine kinase (ATP:guanosine 5'-phosphotransferase), a kinase that is involved in guanosine salvage pathways, has been cloned by using the N-terminal amino acid sequence of the purified protein. The cloned chromosomal fragment containing the gsk gene was sequenc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.22.6959-6964.1997
更新日期:1997-11-01 00:00:00
abstract::SopE is a bacteriophage-encoded effector protein of Salmonella enterica serovar Typhimurium that is translocated into the cytosol of eukaryotic cells by a type III secretion system (TTSS) (W.-D. Hardt, H. Urlaub, and J. E. Galán, Proc. Natl. Acad. Sci. USA 95:2574-2579, 1998; M. W. Wood, R. Rosqvist, P. B. Mullan, M. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.24.7279-7284.2003
更新日期:2003-12-01 00:00:00
abstract::Bacterial Gre factors associate with RNA polymerase (RNAP) and stimulate intrinsic cleavage of the nascent transcript at the active site of RNAP. Biochemical and genetic studies to date have shown that Escherichia coli Gre factors prevent transcriptional arrest during elongation and enhance transcription fidelity. Fur...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00086-11
更新日期:2011-06-01 00:00:00
abstract::The Mg-2+-Sarkosyl crystals (M band) procedure was used to study the effect of ribonuclease (RNase) A on the association of Escherichia coli deoxyribonucleic acid (DNA) with membrane. Incubation of gently prepared cell extracts with RNase results in the release of DNA from membrane. This effect appears to result from ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.122.2.592-598.1975
更新日期:1975-05-01 00:00:00
abstract::The two-partner secretion system ExlBA, expressed by strains of Pseudomonas aeruginosa belonging to the PA7 group, induces hemorrhage in lungs due to disruption of host cellular membranes. Here we demonstrate that the exlBA genes are controlled by a pathway consisting of cAMP and the virulence factor regulator (Vfr). ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00135-18
更新日期:2018-05-24 00:00:00
abstract::Lactate dehydrogenase (LDH) was studied in phage-propagating strains 29, 3A, 6, 81, and 42D of Staphylococcus aureus selected from the five groups in the International-Blair series. Cells were cultivated in Brain Heart Infusion (Difco) under nearly anaerobic conditions and were harvested near the end of the log phase....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.1.74-80.1968
更新日期:1968-01-01 00:00:00
abstract::Dominance tests in diploids have confirmed that the product of the tyrR gene is involved in a negative control system affecting the synthesis of both 3-deoxy-d-arabinoheptulosonic acid 7-phosphate (DAHP) synthetase (tyr) and DAHP synthetase (phe). Some tyrR mutants are derepressed for the synthesis of both DAHP synthe...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.115.3.1145-1150.1973
更新日期:1973-09-01 00:00:00
abstract::A series of mutations of the P1 plasmid prophage that lead to increased copy number was isolated and analyzed. The copy number of the mutants was elevated at least five- to eightfold relative to wild-type P1, as determined by single-cell resistance to antibiotics, activity of enzymes, content of superhelical DNA, and ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.150.3.1329-1339.1982
更新日期:1982-06-01 00:00:00
abstract::Sigma 54-dependent transcriptional activators such as Escherichia coli NtrC, Rhizobium meliloti NifA, and Rhizobium leguminosarum DctD share similar central and carboxy-terminal domains but differ in the structure and function of their amino-terminal domains. We have deleted the amino-terminal and carboxy-terminal dom...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.4.1428-1431.1992
更新日期:1992-02-01 00:00:00
abstract::Transmission electron microscopy was used to examine active ribosomal ribonucleic acid (rRNA) genes in two strains of Escherichia coli: N2077, deficient in the enzyme responsible for proper cleavage of the 16S sequence from the elongating nascent rRNA transcript; and N2076, functional in ribonuclease (RNase) III activ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.132.2.718-722.1977
更新日期:1977-11-01 00:00:00
abstract::The carbon dioxide requirement of 32 strains of rumen bacteria, representing 11 different species, was studied in detail. Increasing concentrations of CO(2) were added as NaHCO(3) to a specially prepared CO(2)-free medium which was tubed and inoculated under nitrogen. Prior depletion of CO(2) in the inoculum was found...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.1.70-76.1971
更新日期:1971-01-01 00:00:00
abstract::Pulse labeling of deoxyribonucleic acid (DNA) and radioautography have been used to study the effect of growth rate on nuclear replication in Aspergillus nidulans. When conidia were germinated in media supporting a fast growth rate, the radioactive pulse labeled either all of the nuclei in a cell or none of them. At s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.6.2275-2281.1968
更新日期:1968-06-01 00:00:00
abstract::We have cloned the Micromonospora viridifaciens neuraminidase (EC 3.2.1.18) gene (nedA) in Streptomyces lividans. This was accomplished by using the vector pIJ702 and BglII-BclI libraries of M. viridifaciens chromosomal inserts created in S. lividans. The libraries were screened for the expression of neuraminidase by ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.21.6896-6903.1992
更新日期:1992-11-01 00:00:00
abstract::The replication order of markers was studied in Bacillus subtilis strains bearing the trpE26 mutation by the use of the density transfer technique. An important difference in this order was observed in comparison with that of strain 168 T-. All markers tested of a chromosome segment extending from trpD to ilvA replica...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.122.3.886-898.1975
更新日期:1975-06-01 00:00:00
abstract::Growing cells of Campylobacter coli and C. jejuni were naturally transformed by naked DNA without the requirement for any special treatment. Transformation frequencies for homologous chromosomal DNA were approximately 10(-3) transformants per recipient cell in C. coli and 10(-4) in C. jejuni. Maximum competence was fo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.2.949-955.1990
更新日期:1990-02-01 00:00:00
abstract::spo0A and spo0H are needed for the initiation of sporulation and for the development of genetic competence in Bacillus subtilis. Transcription of spo0A initiates from two promoters, Pv and Ps. Pv is active during vegetative growth and is recognized by RNA polymerase containing sigma A. Expression from Ps increases dur...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.12.3812-3815.1994
更新日期:1994-06-01 00:00:00
abstract::A system for high-level expression of heparinase I, heparinase II, heparinase III, chondroitinase AC, and chondroitinase B in Flavobacterium heparinum is described. hepA, along with its regulatory region, as well as hepB, hepC, cslA, and cslB, cloned downstream of the hepA regulatory region, was integrated in the chro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.12.3242-3252.2002
更新日期:2002-06-01 00:00:00
abstract::Vibrio parahaemolyticus possesses two types of flagella, polar and lateral, powered by distinct energy sources, which are derived from the sodium and proton motive forces, respectively. Although proton-powered flagella in Escherichia coli and Salmonella enterica serovar Typhimurium have been extensively studied, the m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.4.1035-1045.2000
更新日期:2000-02-01 00:00:00
abstract::An R plasmid, R100-1, was mapped previously (Yoshikawa, 1974) by transduction from an integratively suppressed Hfr strain to a recipient with a mutation in gene dnaA. By this method various types of transductants of plasmid R100-1 that exist autonomously or in the integrated state were obtained. Seventy-one such trans...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.3.1097-1100.1975
更新日期:1975-12-01 00:00:00
abstract::Erwinia rhapontici is able to convert sucrose into isomaltulose (palatinose, 6-O-alpha-D-glucopyranosyl-D-fructose) and trehalulose (1-O-alpha-D-glucopyranosyl-D-fructose) by the activity of a sucrose isomerase. These sucrose isomers cannot be metabolized by plant cells and most other organisms and therefore are possi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.8.2425-2430.2001
更新日期:2001-04-01 00:00:00
abstract::A widely distributed family of small regulators, called C proteins, controls a subset of restriction-modification systems. The C proteins studied to date activate transcription of their own genes and that of downstream endonuclease genes; this arrangement appears to delay endonuclease expression relative to that of th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.2.488-497.2005
更新日期:2005-01-01 00:00:00
abstract::A number of polyacrylamide gel systems and solubilization procedures were studied to define the number and nature of "major" polypeptide bands in the outer membrane of Pseudomonas aeruginosa. It was shown that five of the eight major outer membrane proteins were "heat modifiable" in that their mobility on sodium dodec...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.3.902-910.1979
更新日期:1979-12-01 00:00:00
abstract::A genetic system was developed to investigate the supercoil structure of bacterial chromosomes. New res-carrying transposons were derived from MudI1734 (MudJr1 and MudJr2) and Tn10 (Tn10dGn). The MudJr1 and MudJr2 elements each have a res site in opposite orientation so that when paired with a Tn10dGn element in the s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.10.2825-2835.1996
更新日期:1996-05-01 00:00:00
abstract::Replication initiator proteins in bacteria not only allow DNA replication but also often regulate the rate of replication initiation as well. The regulation is mediated by limiting the synthesis or availability of initiator proteins. The applicability of this principle is demonstrated here for RctB, the replication in...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.21.7167-7175.2005
更新日期:2005-11-01 00:00:00