Stabilization of the pertussis toxin secretion apparatus by the C terminus of PtlD.

Abstract:

:Pertussis toxin (PT) is secreted from Bordetella pertussis by a type IV secretion system, known as the Ptl transporter, that comprises nine different proteins, PtlA to PtlI. In this study, we found that PtlD is required for the stability of three Ptl proteins, PtlE, PtlF, and PtlH. A region limited to the C-terminal 72 amino acids of PtlD (amino acids 392 to 463) was sufficient for maintaining the stability of PtlE, PtlF, and PtlH, although this region was not sufficient to support secretion of the toxin. Further analysis demonstrated that a stretch of 10 amino acids at the C-terminal end of PtlD (amino acids 425 to 434) contributes to transporter stability.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Verma A,Cheung AM,Burns DL

doi

10.1128/JB.01106-08

subject

Has Abstract

pub_date

2008-11-01 00:00:00

pages

7285-90

issue

21

eissn

0021-9193

issn

1098-5530

pii

JB.01106-08

journal_volume

190

pub_type

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