Ultrastructural analysis during germination and outgrowth of Bacillus subtilis spores.

Abstract:

:Electron microscopy of thin sections of dormant and germinating spores of Bacillus subtilis 168 revealed a progressive change in the structure of the cortex, outer spore coat, and inner spore coat. The initial changes were observed in the cortex region, which showed a loose fibrous network within 10 min of germination, and in the outer spore coat, which began to be sloughed off. The permeability of the complex outer spore layers was modified within 10 min, since, at this time, the internal structures of the spore coat were readily stainable. A nicking degradation action of the laminated inner spore coat began at 20 min, and this progressed for the next 20 min leading to the loosening of the inner spore coat. By 30 min, the outer spore coat showed signs of disintegration, and at 40 min, both the outer and inner spore coats were degraded extensively. At 30 to 40 min, a period just preceding net deoxyribonucleic acid synthesis, mesosomes became very prominent in the inner spore core and the cell wall began to thicken around the spore core. At 50 min, an emerging cell was observed, and by 60 min, there was clear evidence for elongation of the emerging cell and the presence of two nuclear bodies. At 90 min, elongation had been followed by the first cell division. There was evidence for spore coat fragments at the opposite poles of the dividing cell.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Santo LY,Doi RH

doi

10.1128/JB.120.1.475-481.1974

subject

Has Abstract

pub_date

1974-10-01 00:00:00

pages

475-81

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

120

pub_type

杂志文章
  • A zinc finger protein from Candida albicans is involved in sucrose utilization.

    abstract::A sucrose-inducible alpha-glucosidase activity that hydrolyzes sucrose in Candida albicans has been demonstrated previously. The enzyme is assayable in whole cells and was inhibited by both sucrose and maltose. A C. albicans gene (CASUC1) that affects sucrose utilization and alpha-glucosidase activity was cloned by ex...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.1.222-232.1992

    authors: Kelly R,Kwon-Chung KJ

    更新日期:1992-01-01 00:00:00

  • Identification of an Escherichia coli gene homologous to virR, a regulator of Shigella virulence.

    abstract::Virulence in Shigella spp., as well as in strains of enteroinvasive Escherichia coli, is regulated by growth temperature. Previously, virR had been identified as the gene controlling the temperature-regulated expression of Shigella virulence. Since Shigella spp. and E. coli are also known to share greater than 90% DNA...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.5.2879-2881.1989

    authors: Hromockyj AE,Maurelli AT

    更新日期:1989-05-01 00:00:00

  • Studies with a gluconate-6-phosphate dehydrogenase mutant in Escherichia coli strain C.

    abstract::A map location of the gluconate-6-phosphate dehydrogenase (gnd) marker was estimated in Escherichia coli C at approximately 46 min by P1 transduction. The gnd locus appears to lie between the co-transducible histidine and prophage P2 location I markers. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.116.2.1056-1058.1973

    authors: Robbins RS,Kelly B

    更新日期:1973-11-01 00:00:00

  • Isolation and characterization of lipopolysaccharides from cell walls of blue-green algae of the genus Phormidium.

    abstract::Lipopolysaccharide (LPS) fractions were isolated from three species of blue-green algae of the genus Phormidium, namely, P. africanum, P. laminosum, and P. uncinatum, by using a phenol-water procedure followed by exhaustive extraction with ammonium oxalate. The materials obtained were shown to be closely related bioch...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.130.1.1-3.1977

    authors: Mikheyskaya LV,Ovodova RG,Ovodov YS

    更新日期:1977-04-01 00:00:00

  • A ction of cycloheximide on Zygosaccharomyces soja.

    abstract::Tsukada, Yoji (Kyoto University, Kyoto, Japan), Tsunetake Sugimori, Kazutami Imai, and Hideo Katagiri. Action of cycloheximide on Zygosaccharomyces soja. J. Bacteriol. 83:70-75. 1962.-Cycloheximide is known to inhibit the growth of some species of Saccharomyces and other fungi. A new type of action of this antibiotic,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.1.70-75.1962

    authors: TSUKADA Y,SUGIMORI T,IMAI K,KATAGIRI H

    更新日期:1962-01-01 00:00:00

  • Genetic co-regulation of galactose and melibiose utilization in Saccharomyces.

    abstract::The gal3 mutation of Saccharomyces, which is associated with an impairment in the utilization of galactose, has been shown to be pleiotropic, causing similar impairments in the utilization of melibiose and maltose. Milibiose utilization and alpha-galactosidase production are directly controlled by the galactose regula...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.125.1.33-41.1976

    authors: Kew OM,Douglas HC

    更新日期:1976-01-01 00:00:00

  • Partial purification and properties of D-desthiobiotin synthetase from Escherichia coli.

    abstract::d-Desthiobiotin synthetase, an enzyme that catalyzes the synthesis of d-desthiobiotin from dl-7,8-diaminopelargonic acid and HCO(3) (-), was purified 100-fold from cells of a biotin mutant strain of Escherichia coli. Adenosine triphosphate and Mg(2+) were shown, especially in purified extracts, to be obligatory for en...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.104.2.726-733.1970

    authors: Cheeseman P,Pai CH

    更新日期:1970-11-01 00:00:00

  • Multicellular stalk-like structures in Saccharomyces cerevisiae.

    abstract::Stalk formation is a novel pattern of multicellular organization. Yeast cells which survive UV irradiation form colonies that grow vertically to form very long (0.5 to 3.0 cm) and thin (0.5 to 4 mm in diameter) multicellular structures. We describe the conditions required to obtain these stalk-like structures reproduc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.15.3992-3996.1998

    authors: Engelberg D,Mimran A,Martinetto H,Otto J,Simchen G,Karin M,Fink GR

    更新日期:1998-08-01 00:00:00

  • Ribonucleic acid synthesis in T2-infected Escherichia coli during "stringent" control.

    abstract::The leucine auxotroph Escherichia coli 2961 exhibited stringent control of net ribonucleic acid (RNA) synthesis during amino acid starvation. After leucine was exhausted from the medium, the rate of uracil incorporation into RNA rapidly decreased to 2 to 4% of the prestarvation value. Infection of the starved cells wi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.1.111-116.1968

    authors: Sharp K,Green MH

    更新日期:1968-07-01 00:00:00

  • Identification of rhtX and fptX, novel genes encoding proteins that show homology and function in the utilization of the siderophores rhizobactin 1021 by Sinorhizobium meliloti and pyochelin by Pseudomonas aeruginosa, respectively.

    abstract::Rhizobactin 1021 is a hydroxymate siderophore produced by the soil bacterium Sinorhizobium meliloti 2011. A regulon comprising rhtA, encoding the outer membrane receptor protein for the ferrisiderophore; the biosynthesis operon rhbABCDEF; and rhrA, the Ara-C-like regulator of the receptor and biosynthesis genes has be...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.10.2996-3005.2004

    authors: Cuív PO,Clarke P,Lynch D,O'Connell M

    更新日期:2004-05-01 00:00:00

  • Analysis of the peptidoglycan structure of Bacillus subtilis endospores.

    abstract::Peptidoglycan was prepared from purified Bacillus subtilis spores of wild-type and several mutant strains. Digestion with muramidase resulted in cleavage of the glycosidic bonds adjacent to muramic acid replaced by peptide or alanine side chains but not the bonds adjacent to muramic lactam. Reduction of the resulting ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.22.6451-6458.1996

    authors: Popham DL,Helin J,Costello CE,Setlow P

    更新日期:1996-11-01 00:00:00

  • Nature of the promoter activated by C.PvuII, an unusual regulatory protein conserved among restriction-modification systems.

    abstract::A widely distributed family of small regulators, called C proteins, controls a subset of restriction-modification systems. The C proteins studied to date activate transcription of their own genes and that of downstream endonuclease genes; this arrangement appears to delay endonuclease expression relative to that of th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.2.488-497.2005

    authors: Knowle D,Lintner RE,Touma YM,Blumenthal RM

    更新日期:2005-01-01 00:00:00

  • Purification and properties of pyridoxal-5'-phosphate-dependent histidine decarboxylases from Klebsiella planticola and Enterobacter aerogenes.

    abstract::Histidine decarboxylases from Klebsiella planticola and Enterobacter aerogenes were purified to homogeneity and compared with the histidine decarboxylase from Morganella morganii. All three enzymes required pyridoxal 5'-phosphate as a coenzyme, showed optimal activity at pH 6.5, decarboxylated only histidine among the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.9.3963-3968.1987

    authors: Guirard BM,Snell EE

    更新日期:1987-09-01 00:00:00

  • Periplasmic structure of frozen-etched and negatively stained cells of Bacillus licheniformis as correlated with penicillinase formation.

    abstract::Bacillus licheniformis strain 749/C (constitutive for penicillinase formation) and uninduced cells of strain 749 (penicillinase-inducible) were examined after freezeetching. In the early stationary phase, strain 749/C organisms had clusters of vesicles (30 to 40 nm in diameter) on the outer surface of the plasma membr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.2.1002-1009.1969

    authors: Ghosh BK,Lampen JO,Remsen CC

    更新日期:1969-11-01 00:00:00

  • Genes coding for a new pathway of aerobic benzoate metabolism in Azoarcus evansii.

    abstract::A new pathway for aerobic benzoate oxidation has been postulated for Azoarcus evansii and for a Bacillus stearothermophilus-like strain. Benzoate is first transformed into benzoyl coenzyme A (benzoyl-CoA), which subsequently is oxidized to 3-hydroxyadipyl-CoA and then to 3-ketoadipyl-CoA; all intermediates are CoA thi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.22.6301-6315.2002

    authors: Gescher J,Zaar A,Mohamed M,Schägger H,Fuchs G

    更新日期:2002-11-01 00:00:00

  • Regulatory effect of hydrogen on nitrogenase activity of the blue-green alga (cyanobacterium) Nostoc muscorum.

    abstract::Preincubation of the blue-green alga (cyanobacterium) Nostoc muscorum under an atmosphere of argon plus acetylene in the light led to a greater than fourfold increase of light-induced hydrogen evolution and to a 50% increase of acetylene reduction, as compared to cells that had not been preconditioned. The basic and t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.141.3.1037-1040.1980

    authors: Scherer S,Kerfin W,Böger P

    更新日期:1980-03-01 00:00:00

  • Detachment characteristics and oxacillin resistance of Staphyloccocus aureus biofilm emboli in an in vitro catheter infection model.

    abstract::Catheter-related bloodstream infections due to Staphylococcus aureus are of increasing clinical importance. The pathophysiological steps leading to colonization and infection, however, are still incompletely defined. We observed growth and detachment of S. aureus biofilms in an in vitro catheter-infection model by usi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.14.4486-4491.2004

    authors: Fux CA,Wilson S,Stoodley P

    更新日期:2004-07-01 00:00:00

  • Divergence among genes encoding the elongation factor Tu of Yersinia Species.

    abstract::Elongation factor Tu (EF-Tu), encoded by tuf genes, carries aminoacyl-tRNA to the ribosome during protein synthesis. Duplicated tuf genes (tufA and tufB), which are commonly found in enterobacterial species, usually coevolve via gene conversion and are very similar to one another. However, sequence analysis of tuf gen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01067-08

    authors: Isabel S,Leblanc E,Boissinot M,Boudreau DK,Grondin M,Picard FJ,Martel EA,Parham NJ,Chain PS,Bader DE,Mulvey MR,Bryden L,Roy PH,Ouellette M,Bergeron MG

    更新日期:2008-11-01 00:00:00

  • DNA sequence and complementation analysis of a mutation in the rplX gene from Escherichia coli leading to loss of ribosomal protein L24.

    abstract::A mutation in Escherichia coli leads to the loss of ribosomal protein L24, severely impaired growth, and a temperature-sensitive phenotype. The mutation was shown to be in rplX, the gene for protein L24, and was due to the alteration of an AAA codon to a TAA stop codon at position 61 in rplX that resulted in a 20-amin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.163.3.890-894.1985

    authors: Nishi K,Dabbs ER,Schnier J

    更新日期:1985-09-01 00:00:00

  • Iron respiration-driven proton translocation in aerobic bacteria.

    abstract::Washed cell suspensions of Aquaspirillum magnetotacticum MS-1, A. itersonii E12639, Bacillus subtilis 6633, and Escherichia coli CSH27 translocated protons in response to the added oxidant O2 or NO3-, with triphenylmethylphosphonium bromide as the permeant ion. Iron respiration-driven proton translocation was observed...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.167.2.729-731.1986

    authors: Short KA,Blakemore RP

    更新日期:1986-08-01 00:00:00

  • IDENTITY OF THE PINK-PIGMENTED METHANOL-OXIDIZING BACTERIA AS VIBRIO EXTORQUENS.

    abstract::Stocks, Peter K. (Louisiana State University, Baton Rouge), and C. S. McCleskey. Identity of the pink-pigmented methanol-oxidizing bacteria as Vibrio extorquens. J. Bacteriol. 88:1065-1070. 1964.-Pink-pigmented bacteria isolated from enrichment cultures of methane oxidizers were found to possess similar morphological,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.4.1065-1070.1964

    authors: STOCKS PK,MCCLESKEY CS

    更新日期:1964-10-01 00:00:00

  • Genetic studies on ribose 5-phosphate isomerase mutants of Escherichia coli K-12.

    abstract::A gene for the constitutive ribosephosphate isomerase (rpiA) is highly cotransducible with serA at 56.2 min on the genetic linkage map of Escherichia coli K-12. Suppression of ribosephosphate isomerase A-negative mutants can occur by a regulator gene mutation permitting constitutive synthesis of the normally inducible...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.118.3.1183-1185.1974

    authors: Skinner AJ,Cooper RA

    更新日期:1974-06-01 00:00:00

  • Role of a sugar-lipid intermediate in colanic acid synthesis by Escherichia coli.

    abstract::Membrane fractions from a lon strain of Escherichia coli but not a wild-type strain catalyze the incorporation of fucose from guanosine 5'-diphosphate-fucose into a lipid and into polymeric material. Both incorporation reactions specifically require only uridine 5'-diphosphate (UDP)-glucose. The sugar lipid was shown ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.129.1.225-236.1977

    authors: Johnson JG,Wilson DB

    更新日期:1977-01-01 00:00:00

  • SIGNIFICANCE OF SALMONELLAE ISOLATED FROM APPARENTLY HEALTHY MICE.

    abstract::Morello, Josephine A. (Boston University School of Medicine, Boston, Mass.), Teresa A. DiGenio, and Edgar E. Baker. Significance of salmonellae isolated from apparently healthy mice. J. Bacteriol. 89:1460-1464. 1965.-Three species of Salmonella were isolated from three groups of apparently healthy laboratory mice. The...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.6.1460-1464.1965

    authors: MORELLO JA,DIGENIO TA,BAKER EE

    更新日期:1965-06-01 00:00:00

  • Population genetic analysis of Helicobacter pylori by multilocus enzyme electrophoresis: extensive allelic diversity and recombinational population structure.

    abstract::Genetic diversity and relationships in 74 Helicobacter pylori isolates recovered from patients assigned to distinct clinical categories were estimated by examination of allelic variation in six genes encoding metabolic housekeeping enzymes by multilocus enzyme electrophoresis. Seventy-three distinct allele profiles, r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.13.3934-3938.1996

    authors: Go MF,Kapur V,Graham DY,Musser JM

    更新日期:1996-07-01 00:00:00

  • Changes in composition, biosynthesis, and physical state of membrane lipids occurring upon aging of Mycoplasma hominis cultures.

    abstract::During the progression of Mycoplasma hominis cultures from the early logarithmic phase to the stationary phase of growth, the total phospholipid content of the cell membranes decreased. Measurement of the amount of the various phospholipids during the growth cycle showed that a decrease in the phosphatidylglycerol (PG...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.121.2.631-639.1975

    authors: Rottem S,Greenberg AS

    更新日期:1975-02-01 00:00:00

  • Role of ubiquinone in hydrogen-dependent electron transport in Rhizobium japonicum.

    abstract::Direct evidence for the involvement of ubiquinone in H2 oxidation by Rhizobium japonicum was demonstrated; H2 reduced ca. 80% of the extractable ubiquinone. The inhibitor 2-n-heptyl-4-hydroxyquinoline-N-oxide blocked electron transport at a site between ubiquinone and the cytochromes. The results showed that no cytoch...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.161.2.775-777.1985

    authors: O'Brian MR,Maier RJ

    更新日期:1985-02-01 00:00:00

  • High-level production of -galactosidase by Escherichia coli merodiploids.

    abstract::Two merodiploids of Escherichia coli that contain genes for the lac operon on both chromosome and episome were tested for production of lac enzymes after growth on various carbon sources. The specific activity of beta-galactosidase (and of thiogalactoside transacetylase) was about twice that from haploid cells when gr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.112.2.856-860.1972

    authors: Fowler AV

    更新日期:1972-11-01 00:00:00

  • Analysis of the activity and regulon of the two-component regulatory system composed by Cjj81176_1484 and Cjj81176_1483 of Campylobacter jejuni.

    abstract:UNLABELLED:Campylobacter jejuni is a leading cause of bacterial diarrheal disease and a frequent commensal of the intestinal tract in poultry and other animals. For optimal growth and colonization of hosts, C. jejuni employs two-component regulatory systems (TCSs) to monitor environmental conditions and promote proper ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02564-14

    authors: Luethy PM,Huynh S,Parker CT,Hendrixson DR

    更新日期:2015-05-01 00:00:00

  • Glycerol-induced unraveling of the tight helical conformation of Escherichia coli type 1 fimbriae.

    abstract::Glycerol was found to unravel the helical conformation of Escherichia coli type 1 fimbriae without appreciable depolymerization. The linearized fimbrial polymers have a diameter of 2 nm, react strongly with a monoclonal antibody directed at an inaccessible epitope on native fimbriae, and display greater mannose-bindin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.15.5145-5148.1992

    authors: Abraham SN,Land M,Ponniah S,Endres R,Hasty DL,Babu JP

    更新日期:1992-08-01 00:00:00